id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-300640-9pvhaz8q	Parackova, Zuzana	Disharmonic Inflammatory Signatures in COVID-19: Augmented Neutrophils’ but Impaired Monocytes’ and Dendritic Cells’ Responsiveness	2020-09-29		.txt	text/plain	5975	344	47	We demonstrate the impaired functionality of COVID-19 dendritic cells and monocytes, particularly their low expression of maturation markers, increased PD-L1 levels, and their inability to upregulate phenotype upon stimulation. Ex vivo stimulation of peripheral whole blood with lipopolysaccharide (LPS) led to a rapid increase in surface degranulation markers CD11b and CD66b, and decrease in CD62L, a lectin involved in granulocyte trafficking, on both patient and healthy donor neutrophils ( Figure 1C and Figure S1C ). Only the COVID-19, but not the healthy neutrophils, were able to increase the production of IL-1β and TNFα upon ssRNA stimulation in comparison with untreated cells ( Figure 2B ) indicating a pro-inflammatory bias, possibly due to priming with SARS-CoV-2 or excessive cytokine/chemokine stimulation. In contrast to monocytes and DCs, COVID-19 neutrophils expressed significantly decreased levels of PD-L1 and their stimulation with ssRNA led to elevated production of proinflammatory cytokines.	./cache/cord-300640-9pvhaz8q.txt	./txt/cord-300640-9pvhaz8q.txt