id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-337220-yv7qdvzi	Demeke, Addis	Biosensor and molecular-based methods for the detection of human coronaviruses: A review	2020-09-08		.txt	text/plain	2215	128	39	This assay involves simultaneous 130 reverse transcription and isothermal amplification using loop-mediated amplification (RT-131 LAMP) for RNA, followed by Cas12 detection of predefined coronavirus sequences, after which 132 cleavage of a reporter molecule confirms detection of the E and N genes of SARS-CoV-2. Rapid lateral flow-based assays for anti-COVID-19 antibodies (IgM and IgG) are under 147 development which will play an important role in the epidemiological investigation of the 148 disease [9] . Therefore, the convalescent plasma has been used as 155 therapy for the treatment of critically ill COVID-19 patients [26, 27] The biosensor was developed by using a spike protein of SARS-CoV-2 immobilized onto the 237 FET graphene sheet (a two-dimensional sheet of hexagonal oriented carbon atom) with 1-pyrene 238 butyric acid N-hydroxy succinimide ester (PBASE) (Figure 1) . Rapid and visual detection of 2019 novel coronavirus (SARS-CoV-2) by a reverse transcription 473 loop-mediated isothermal amplification assay	./cache/cord-337220-yv7qdvzi.txt	./txt/cord-337220-yv7qdvzi.txt