id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-268081-ytx6sf3x	Guionie, Olivier	An experimental study of the survival of turkey coronavirus at room temperature and +4°C	2013-04-22		.txt	text/plain	4051	195	55	As TCoV does not induce lesions in the embryo, virus replication in the jejunum and ileum of the embryos was detected 4 days post inoculation, using RNA extraction and a real-time reverse transcriptase-polymerase chain reaction based on the nucleocapsid gene. However, infectious virus was still detected after 20 days storage at the cooler temperatures, a finding that suggests prolonged survival of Fr TCoV and easier transmission between poultry farms in a cool environment are possible. The first duplicates harvested after different times of storage at the different temperatures were used to test for the presence of residual infectious virus in the sample by inoculating SPF embryonated turkey eggs as described above. However, when the same samples were inoculated into embryonated SPF turkey eggs, viral RNA was detected in the digestive tracts of the inoculated embryos after 0 days of storage (27.5 Ct), but not after 42 or 40 days ( 40 Ct) at room temperature or '48C, respectively.	./cache/cord-268081-ytx6sf3x.txt	./txt/cord-268081-ytx6sf3x.txt