id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-276338-wpo1eyeg	Hesse, Amke	In toxic demyelination oligodendroglial cell death occurs early and is FAS independent	2009-10-22		.txt	text/plain	5481	288	48	During the initial phase of the cuprizone diet (days 6 and 10), but not during later stages (day 21), activated caspase 3 can be detected in apoptotic oligodendrocytes, suggesting that caspase 3-independent pathways trigger oligodendroglial cell death in later stages. In line with the notion of continuous oligodendroglial cell death, we found a significant decrease in the number of Nogo-A-positive mature oligodendrocytes in mice fed with cuprizone after 10 (540 ± 187 cells/mm 2 ) and 21 days (17 ± 15 cells/mm 2 ) compared to untreated controls (1797 ± 404 cells/mm 2 ) (Fig. 2b) . The number of apoptotic cells, Nogo-A-positive oligodendrocytes, activated caspase 3-expressing oligodendrocytes, macrophages and astrocytes, as well as the extent of demyelination, was determined on days 4, 6 and 10 and in mice fed with a cuprizone-free diet (controls). In the cuprizone model, apoptotic oligodendrocytes express activated caspase 3, and this is associated with a significant increase of FAS mRNA in the corpus callosum.	./cache/cord-276338-wpo1eyeg.txt	./txt/cord-276338-wpo1eyeg.txt