id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-295633-vkjcheaz	Hao, Xin‐yan	The characteristics of hDPP4 transgenic mice subjected to aerosol MERS coronavirus infection via an animal nose‐only exposure device	2019-10-30		.txt	text/plain	5108	255	50	After infection, we analyzed the mouse characteristics of weight loss, survival, viral replication, tissue pathology, viral antigen distribution, and cytokine and chemokine profiles, which provide additional data to investigate the pathogenesis of MERS-CoV-induced disease and evaluate relevant therapeutics and vaccines. Instillation control DMEM suspension To analyze clinical signs, weight loss, and survival On days 3, 5, 7, and 9 postinfection, three animals randomly selected from each group underwent necropsy to obtain tissue specimens for assessing viral distribution, associated histopathology, and cytokine levels using quantitative reverse transcription-PCR (qRT-PCR), hematoxylin and eosin (H&E) staining, immunohistochemistry (IHC), and enzyme-linked immunosorbent assay (ELISA). *P < .05, **P < .01, ***P < .001, and ****P < .0001 Mice in the instillation group died acutely or were euthanized when they researched 25% weight loss; these mice had a 0% survival rate by day 5, so no tissue lesion results were available on days 7 and 9 mice infected with MERS-CoV via the aerosol inhalation or intranasal instillation route, but no obvious lesions were found in other tissues.	./cache/cord-295633-vkjcheaz.txt	./txt/cord-295633-vkjcheaz.txt