Carrel name: keyword-denv-cord Creating study carrel named keyword-denv-cord Initializing database file: cache/cord-002413-795wuqz5.json key: cord-002413-795wuqz5 authors: Balinsky, Corey A.; Schmeisser, Hana; Wells, Alexandra I.; Ganesan, Sundar; Jin, Tengchuan; Singh, Kavita; Zoon, Kathryn C. title: IRAV (FLJ11286), an Interferon-Stimulated Gene with Antiviral Activity against Dengue Virus, Interacts with MOV10 date: 2017-02-14 journal: J Virol DOI: 10.1128/jvi.01606-16 sha: doc_id: 2413 cord_uid: 795wuqz5 file: cache/cord-002085-e7xwb03g.json key: cord-002085-e7xwb03g authors: Yamashita, Akifumi; Sakamoto, Tetsuya; Sekizuka, Tsuyoshi; Kato, Kengo; Takasaki, Tomohiko; Kuroda, Makoto title: DGV: Dengue Genographic Viewer date: 2016-06-07 journal: Front Microbiol DOI: 10.3389/fmicb.2016.00875 sha: doc_id: 2085 cord_uid: e7xwb03g file: cache/cord-276364-zyw5aukk.json key: cord-276364-zyw5aukk authors: Wong, Ho Him; Sanyal, Sumana title: Manipulation of autophagy by (+) RNA viruses date: 2019-08-08 journal: Semin Cell Dev Biol DOI: 10.1016/j.semcdb.2019.07.013 sha: doc_id: 276364 cord_uid: zyw5aukk file: cache/cord-002426-5e1xn7kj.json key: cord-002426-5e1xn7kj authors: Falcón-Lezama, Jorge Abelardo; Santos-Luna, René; Román-Pérez, Susana; Martínez-Vega, Ruth Aralí; Herrera-Valdez, Marco Arieli; Kuri-Morales, Ángel Fernando; Adams, Ben; Kuri-Morales, Pablo Antonio; López-Cervantes, Malaquías; Ramos-Castañeda, José title: Analysis of spatial mobility in subjects from a Dengue endemic urban locality in Morelos State, Mexico date: 2017-02-22 journal: PLoS One DOI: 10.1371/journal.pone.0172313 sha: doc_id: 2426 cord_uid: 5e1xn7kj file: cache/cord-011012-5mev3otu.json key: cord-011012-5mev3otu authors: Rathore, Abhishek Singh; Sarker, Animesh; Gupta, Rinkoo Devi title: Production and immunogenicity of Fubc subunit protein redesigned from DENV envelope protein date: 2020-03-30 journal: Appl Microbiol Biotechnol DOI: 10.1007/s00253-020-10541-y sha: doc_id: 11012 cord_uid: 5mev3otu file: cache/cord-300648-ixiam7qr.json key: cord-300648-ixiam7qr authors: Zhu, Xun; He, Zhenjian; Yuan, Jie; Wen, Weitao; Huang, Xuan; Hu, Yiwen; Lin, Cuiji; Pan, Jing; Li, Ran; Deng, Haijing; Liao, Shaowei; Zhou, Rui; Wu, Jueheng; Li, Jun; Li, Mengfeng title: IFITM3‐containing exosome as a novel mediator for anti‐viral response in dengue virus infection date: 2014-08-30 journal: Cell Microbiol DOI: 10.1111/cmi.12339 sha: doc_id: 300648 cord_uid: ixiam7qr file: cache/cord-253480-qchrw337.json key: cord-253480-qchrw337 authors: Pu, Jieying; He, Li; Xie, Heping; Wu, Siyu; Li, Yuye; Zhang, Ping; Yang, Zhicong; Huang, Xi title: Antiviral activity of Carbenoxolone disodium against dengue virus infection date: 2016-12-23 journal: J Med Virol DOI: 10.1002/jmv.24571 sha: doc_id: 253480 cord_uid: qchrw337 file: cache/cord-033331-giku34r9.json key: cord-033331-giku34r9 authors: Manrique-Saide, Pablo; Dean, Natalie E.; Halloran, M. Elizabeth; Longini, Ira M.; Collins, Matthew H.; Waller, Lance A.; Gomez-Dantes, Hector; Lenhart, Audrey; Hladish, Thomas J.; Che-Mendoza, Azael; Kirstein, Oscar D.; Romer, Yamila; Correa-Morales, Fabian; Palacio-Vargas, Jorge; Mendez-Vales, Rosa; Pérez, Pilar Granja; Pavia-Ruz, Norma; Ayora-Talavera, Guadalupe; Vazquez-Prokopec, Gonzalo M. title: The TIRS trial: protocol for a cluster randomized controlled trial assessing the efficacy of preventive targeted indoor residual spraying to reduce Aedes-borne viral illnesses in Merida, Mexico date: 2020-10-08 journal: Trials DOI: 10.1186/s13063-020-04780-7 sha: doc_id: 33331 cord_uid: giku34r9 file: cache/cord-278260-3o91v72a.json key: cord-278260-3o91v72a authors: Halstead, Scott B; Katzelnick, Leah title: COVID 19 Vaccines: Should we fear ADE? date: 2020-08-12 journal: J Infect Dis DOI: 10.1093/infdis/jiaa518 sha: doc_id: 278260 cord_uid: 3o91v72a file: cache/cord-272459-w14finxf.json key: cord-272459-w14finxf authors: Heaton, Nicholas S.; Randall, Glenn title: Dengue Virus and Autophagy date: 2011-08-04 journal: Viruses DOI: 10.3390/v3081332 sha: doc_id: 272459 cord_uid: w14finxf file: cache/cord-002179-v8lpw4r7.json key: cord-002179-v8lpw4r7 authors: Viktorovskaya, Olga V.; Greco, Todd M.; Cristea, Ileana M.; Thompson, Sunnie R. title: Identification of RNA Binding Proteins Associated with Dengue Virus RNA in Infected Cells Reveals Temporally Distinct Host Factor Requirements date: 2016-08-24 journal: PLoS Negl Trop Dis DOI: 10.1371/journal.pntd.0004921 sha: doc_id: 2179 cord_uid: v8lpw4r7 file: cache/cord-011390-98xyie7s.json key: cord-011390-98xyie7s authors: Darcy, Andrew Waleluma; Kanda, Seiji; Dalipanda, Tenneth; Joshua, Cynthia; Shimono, Takaki; Lamaningao, Pheophet; Mishima, Nobuyuki; Nishiyama, Toshimasa title: Multiple arboviral infections during a DENV-2 outbreak in Solomon Islands date: 2020-05-15 journal: Trop Med Health DOI: 10.1186/s41182-020-00217-8 sha: doc_id: 11390 cord_uid: 98xyie7s file: cache/cord-270495-2u072mtp.json key: cord-270495-2u072mtp authors: Lokida, Dewi; Lukman, Nurhayati; Salim, Gustiani; Butar-butar, Deni Pepy; Kosasih, Herman; Wulan, Wahyu Nawang; Naysilla, Adhella Menur; Djajady, Yuanita; Sari, Rizki Amalia; Arlinda, Dona; Lau, Chuen-Yen; Karyana, Muhammad title: Diagnosis of COVID-19 in a Dengue-Endemic Area date: 2020-08-05 journal: Am J Trop Med Hyg DOI: 10.4269/ajtmh.20-0676 sha: doc_id: 270495 cord_uid: 2u072mtp file: cache/cord-256732-md1u51va.json key: cord-256732-md1u51va authors: Shrivastava, Gaurav; Valenzuela Leon, Paola Carolina; Calvo, Eric title: Inflammasome Fuels Dengue Severity date: 2020-09-10 journal: Front Cell Infect Microbiol DOI: 10.3389/fcimb.2020.00489 sha: doc_id: 256732 cord_uid: md1u51va file: cache/cord-002581-r7mskri0.json key: cord-002581-r7mskri0 authors: Magnani, Diogo M.; Silveira, Cassia G. T.; Rosen, Brandon C.; Ricciardi, Michael J.; Pedreño-Lopez, Núria; Gutman, Martin J.; Bailey, Varian K.; Maxwell, Helen S.; Domingues, Aline; Gonzalez-Nieto, Lucas; Avelino-Silva, Vivian I.; Trindade, Mateus; Nogueira, Juliana; Oliveira, Consuelo S.; Maestri, Alvino; Felix, Alvina Clara; Levi, José Eduardo; Nogueira, Mauricio L.; Martins, Mauricio A.; Martinez-Navio, José M.; Fuchs, Sebastian P.; Whitehead, Stephen S.; Burton, Dennis R.; Desrosiers, Ronald C.; Kallas, Esper G.; Watkins, David I. title: A human inferred germline antibody binds to an immunodominant epitope and neutralizes Zika virus date: 2017-06-12 journal: PLoS Negl Trop Dis DOI: 10.1371/journal.pntd.0005655 sha: doc_id: 2581 cord_uid: r7mskri0 file: cache/cord-273388-615acz0l.json key: cord-273388-615acz0l authors: He, Miao; Wang, Jingxing; Chen, Limin; Liu, Jing; Zeng, Peibin title: The Impact of Emerging Infectious Diseases on Chinese Blood Safety() date: 2016-11-04 journal: Transfus Med Rev DOI: 10.1016/j.tmrv.2016.10.002 sha: doc_id: 273388 cord_uid: 615acz0l file: cache/cord-274129-vaygaqe5.json key: cord-274129-vaygaqe5 authors: Cheng, Ming Soon; Lau, Suk Hiang; Chan, Kwai Peng; Toh, Chee-Seng; Chow, Vincent T. title: Impedimetric cell-based biosensor for real-time monitoring of cytopathic effects induced by dengue viruses date: 2015-08-15 journal: Biosens Bioelectron DOI: 10.1016/j.bios.2015.03.018 sha: doc_id: 274129 cord_uid: vaygaqe5 file: cache/cord-277547-2vim1wno.json key: cord-277547-2vim1wno authors: Zandi, Keivan; Teoh, Boon-Teong; Sam, Sing-Sin; Wong, Pooi-Fong; Mustafa, Mohd Rais; AbuBakar, Sazaly title: Antiviral activity of four types of bioflavonoid against dengue virus type-2 date: 2011-12-28 journal: Virol J DOI: 10.1186/1743-422x-8-560 sha: doc_id: 277547 cord_uid: 2vim1wno file: cache/cord-011968-abd891ej.json key: cord-011968-abd891ej authors: Lai, Yen-Chung; Chao, Chiao-Hsuan; Yeh, Trai-Ming title: Roles of Macrophage Migration Inhibitory Factor in Dengue Pathogenesis: From Pathogenic Factor to Therapeutic Target date: 2020-06-12 journal: Microorganisms DOI: 10.3390/microorganisms8060891 sha: doc_id: 11968 cord_uid: abd891ej file: cache/cord-025181-eg108wcd.json key: cord-025181-eg108wcd authors: Zheng, Zhihang; Li, Min; Liu, Zhihua; Jin, Xia; Sun, Jin title: Establishment of Murine Infection Models with Biological Clones of Dengue Viruses Derived from a Single Clinical Viral Isolate date: 2020-05-25 journal: Virol Sin DOI: 10.1007/s12250-020-00229-y sha: doc_id: 25181 cord_uid: eg108wcd file: cache/cord-282742-eyukbot7.json key: cord-282742-eyukbot7 authors: Diosa-Toro, Mayra; Urcuqui-Inchima, Silvio; Smit, Jolanda M. title: Arthropod-Borne Flaviviruses and RNA Interference: Seeking New Approaches for Antiviral Therapy date: 2013-02-20 journal: Adv Virus Res DOI: 10.1016/b978-0-12-408116-1.00004-5 sha: doc_id: 282742 cord_uid: eyukbot7 file: cache/cord-286255-ded5t1ai.json key: cord-286255-ded5t1ai authors: Tomashek, Kay M.; Lorenzi, Olga D.; Andújar-Pérez, Doris A.; Torres-Velásquez, Brenda C.; Hunsperger, Elizabeth A.; Munoz-Jordan, Jorge Luis; Perez-Padilla, Janice; Rivera, Aidsa; Gonzalez-Zeno, Gladys E.; Sharp, Tyler M.; Galloway, Renee L.; Glass Elrod, Mindy; Mathis, Demetrius L.; Oberste, M. Steven; Nix, W. Allan; Henderson, Elizabeth; McQuiston, Jennifer; Singleton, Joseph; Kato, Cecilia; García Gubern, Carlos; Santiago-Rivera, William; Cruz-Correa, Jesús; Muns-Sosa, Robert; Ortiz-Rivera, Juan D.; Jiménez, Gerson; Galarza, Ivonne E.; Horiuchi, Kalanthe; Margolis, Harold S.; Alvarado, Luisa I. title: Clinical and epidemiologic characteristics of dengue and other etiologic agents among patients with acute febrile illness, Puerto Rico, 2012–2015 date: 2017-09-13 journal: PLoS Negl Trop Dis DOI: 10.1371/journal.pntd.0005859 sha: doc_id: 286255 cord_uid: ded5t1ai file: cache/cord-297662-slmlhqnb.json key: cord-297662-slmlhqnb authors: Yap, Sally S. L.; Nguyen-Khuong, Terry; Rudd, Pauline M.; Alonso, Sylvie title: Dengue Virus Glycosylation: What Do We Know? date: 2017-07-25 journal: Front Microbiol DOI: 10.3389/fmicb.2017.01415 sha: doc_id: 297662 cord_uid: slmlhqnb file: cache/cord-318120-vfznyyz6.json key: cord-318120-vfznyyz6 authors: Dauner, Allison L.; Mitra, Indrani; Gilliland, Theron; Seales, Sajeewane; Pal, Subhamoy; Yang, Shih-Chun; Guevara, Carolina; Chen, Jiann-Hwa; Liu, Yung-Chuan; Kochel, Tadeusz J.; Wu, Shuenn-Jue L. title: Development of a pan-serotype reverse transcription loop-mediated isothermal amplification assay for the detection of dengue virus date: 2015-05-15 journal: Diagn Microbiol Infect Dis DOI: 10.1016/j.diagmicrobio.2015.05.004 sha: doc_id: 318120 cord_uid: vfznyyz6 file: cache/cord-318614-518giv0m.json key: cord-318614-518giv0m authors: Tsai, Jih-Jin; Liu, Wei-Liang; Lin, Ping-Chang; Huang, Bo-Yi; Tsai, Ching-Yi; Lee, Pei-Yu Alison; Tsai, Yun-Long; Chou, Pin-Hsing; Chung, Simon; Liu, Li-Teh; Chen, Chun-Hong title: A fully automated sample-to-answer PCR system for easy and sensitive detection of dengue virus in human serum and mosquitos date: 2019-07-10 journal: PLoS One DOI: 10.1371/journal.pone.0218139 sha: doc_id: 318614 cord_uid: 518giv0m file: cache/cord-276718-3lujp0oy.json key: cord-276718-3lujp0oy authors: Neeraja, M.; Lakshmi, V.; Lavanya, Vanjari; Priyanka, E.N.; Parida, M.M.; Dash, P.K.; Sharma, Shashi; Rao, P.V. Lakshmana; Reddy, Gopal title: Rapid detection and differentiation of dengue virus serotypes by NS1 specific reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay in patients presenting to a tertiary care hospital in Hyderabad, India date: 2014-10-24 journal: J Virol Methods DOI: 10.1016/j.jviromet.2014.10.005 sha: doc_id: 276718 cord_uid: 3lujp0oy file: cache/cord-326840-piv6h7oq.json key: cord-326840-piv6h7oq authors: Paemanee, Atchara; Hitakarun, Atitaya; Roytrakul, Sittiruk; Smith, Duncan R. title: Screening of melatonin, α-tocopherol, folic acid, acetyl-l-carnitine and resveratrol for anti-dengue 2 virus activity date: 2018-05-16 journal: BMC Res Notes DOI: 10.1186/s13104-018-3417-3 sha: doc_id: 326840 cord_uid: piv6h7oq file: cache/cord-285538-3ah05ijf.json key: cord-285538-3ah05ijf authors: Halstead, Scott B. title: Chapter 19 Pathogenic Exploitation of Fc Activity date: 2014-12-31 journal: Antibody Fc DOI: 10.1016/b978-0-12-394802-1.00019-4 sha: doc_id: 285538 cord_uid: 3ah05ijf file: cache/cord-011026-iapgkz0p.json key: cord-011026-iapgkz0p authors: El-Bitar, Alaa M. H.; Sarhan, Moustafa; Abdel-Rahman, Mohamed A.; Quintero-Hernandez, Veronica; Aoki-Utsubo, Chie; Moustafa, Mohsen A.; Possani, Lourival D.; Hotta, Hak title: Smp76, a Scorpine-Like Peptide Isolated from the Venom of the Scorpion Scorpio maurus palmatus, with a Potent Antiviral Activity Against Hepatitis C Virus and Dengue Virus date: 2019-07-06 journal: Int J Pept Res Ther DOI: 10.1007/s10989-019-09888-2 sha: doc_id: 11026 cord_uid: iapgkz0p file: cache/cord-320091-2lrqubdl.json key: cord-320091-2lrqubdl authors: Badawi, Alaa; Velummailum, Russanthy; Ryoo, Seung Gwan; Senthinathan, Arrani; Yaghoubi, Sahar; Vasileva, Denitsa; Ostermeier, Emma; Plishka, Mikayla; Soosaipillai, Marcel; Arora, Paul title: Prevalence of chronic comorbidities in dengue fever and West Nile virus: A systematic review and meta-analysis date: 2018-07-10 journal: PLoS One DOI: 10.1371/journal.pone.0200200 sha: doc_id: 320091 cord_uid: 2lrqubdl file: cache/cord-295191-xu26mvc3.json key: cord-295191-xu26mvc3 authors: Avirutnan, Panisadee; Mehlhop, Erin; Diamond, Michael S. title: Complement and its role in protection and pathogenesis of flavivirus infections date: 2008-12-30 journal: Vaccine DOI: 10.1016/j.vaccine.2008.11.061 sha: doc_id: 295191 cord_uid: xu26mvc3 file: cache/cord-290385-0smnl70i.json key: cord-290385-0smnl70i authors: Chan, Jasper F.W.; Choi, Garnet K.Y.; Yip, Cyril C.Y.; Cheng, Vincent C.C.; Yuen, Kwok-Yung title: Zika fever and congenital Zika syndrome: An unexpected emerging arboviral disease date: 2016-03-03 journal: J Infect DOI: 10.1016/j.jinf.2016.02.011 sha: doc_id: 290385 cord_uid: 0smnl70i file: cache/cord-317169-qlqavi4t.json key: cord-317169-qlqavi4t authors: Chiow, K.H.; Phoon, M.C.; Putti, Thomas; Tan, Benny K.H.; Chow, Vincent T. title: Evaluation of antiviral activities of Houttuynia cordata Thunb. extract, quercetin, quercetrin and cinanserin on murine coronavirus and dengue virus infection date: 2015-12-19 journal: Asian Pac J Trop Med DOI: 10.1016/j.apjtm.2015.12.002 sha: doc_id: 317169 cord_uid: qlqavi4t file: cache/cord-355489-tkvfneje.json key: cord-355489-tkvfneje authors: Mendez, Jairo A; Usme-Ciro, Jose A; Domingo, Cristina; Rey, Gloria J; Sanchez, Juan A; Tenorio, Antonio; Gallego-Gomez, Juan C title: Phylogenetic history demonstrates two different lineages of dengue type 1 virus in Colombia date: 2010-09-14 journal: Virol J DOI: 10.1186/1743-422x-7-226 sha: doc_id: 355489 cord_uid: tkvfneje file: cache/cord-328661-spxgox52.json key: cord-328661-spxgox52 authors: Yu, Jianhai; Li, Xujuan; He, Xiaoen; Liu, Xuling; Zhong, Zhicheng; Xie, Qian; Zhu, Li; Jia, Fengyun; Mao, Yingxue; Chen, Zongqiu; Wen, Ying; Ma, Danjuan; Yu, Linzhong; Zhang, Bao; Zhao, Wei; Xiao, Weiwei title: Epidemiological and Evolutionary Analysis of Dengue-1 Virus Detected in Guangdong during 2014: Recycling of Old and Formation of New Lineages date: 2019-08-05 journal: Am J Trop Med Hyg DOI: 10.4269/ajtmh.18-0951 sha: doc_id: 328661 cord_uid: spxgox52 file: cache/cord-294842-aesiff1f.json key: cord-294842-aesiff1f authors: Romero-Brey, Inés; Bartenschlager, Ralf title: Membranous Replication Factories Induced by Plus-Strand RNA Viruses date: 2014-07-22 journal: Viruses DOI: 10.3390/v6072826 sha: doc_id: 294842 cord_uid: aesiff1f file: cache/cord-330743-o11d0aa1.json key: cord-330743-o11d0aa1 authors: Yu, Xi; Zhang, Liming; Tong, Liangqin; Zhang, Nana; Wang, Han; Yang, Yun; Shi, Mingyu; Xiao, Xiaoping; Zhu, Yibin; Wang, Penghua; Ding, Qiang; Zhang, Linqi; Qin, Chengfeng; Cheng, Gong title: Broad-spectrum virucidal activity of bacterial secreted lipases against flaviviruses, SARS-CoV-2 and other enveloped viruses date: 2020-05-25 journal: bioRxiv DOI: 10.1101/2020.05.22.109900 sha: doc_id: 330743 cord_uid: o11d0aa1 file: cache/cord-306204-rkher4ly.json key: cord-306204-rkher4ly authors: Pong, Lian Yih; Yew, Peng Nian; Lee, Wai Leng; Lim, Yau Yan; Sharifah, Syed Hassan title: Anti-dengue virus serotype 2 activity of tannins from porcupine dates date: 2020-05-20 journal: Chin Med DOI: 10.1186/s13020-020-00329-7 sha: doc_id: 306204 cord_uid: rkher4ly file: cache/cord-345898-a6vt8kso.json key: cord-345898-a6vt8kso authors: Ren, Linzhu; Peng, Zhiyuan; Chen, Xinrong; Ouyang, Hongsheng title: Live Cell Reporter Systems for Positive-Sense Single Strand RNA Viruses date: 2016-01-04 journal: Appl Biochem Biotechnol DOI: 10.1007/s12010-015-1968-5 sha: doc_id: 345898 cord_uid: a6vt8kso file: cache/cord-353241-ityhcak7.json key: cord-353241-ityhcak7 authors: Zhu, Hanliang; Podesva, Pavel; Liu, Xiaocheng; Zhang, Haoqing; Teply, Tomas; Xu, Ying; Chang, Honglong; Qian, Airong; Lei, Yingfeng; Li, Yu; Niculescu, Andreea; Iliescu, Ciprian; Neuzil, Pavel title: IoT PCR for pandemic disease detection and its spread monitoring date: 2020-01-15 journal: Sens Actuators B Chem DOI: 10.1016/j.snb.2019.127098 sha: doc_id: 353241 cord_uid: ityhcak7 file: cache/cord-307219-okvvajms.json key: cord-307219-okvvajms authors: Lazzarini, Luca; Barzon, Luisa; Foglia, Felice; Manfrin, Vinicio; Pacenti, Monia; Pavan, Giacomina; Rassu, Mario; Capelli, Gioia; Montarsi, Fabrizio; Martini, Simone; Zanella, Francesca; Padovan, Maria Teresa; Russo, Francesca; Gobbi, Federico title: First autochthonous dengue outbreak in Italy, August 2020 date: 2020-09-10 journal: Euro Surveill DOI: 10.2807/1560-7917.es.2020.25.36.2001606 sha: doc_id: 307219 cord_uid: okvvajms file: cache/cord-342157-qjyooq68.json key: cord-342157-qjyooq68 authors: King, Chwan-Chuen; Chao, Day-Yu; Chien, Li-Jung; Chang, Gwong-Jen J; Lin, Ting-Hsiang; Wu, Yin-Chang; Huang, Jyh-Hsiung title: Comparative analysis of full genomic sequences among different genotypes of dengue virus type 3 date: 2008-05-21 journal: Virol J DOI: 10.1186/1743-422x-5-63 sha: doc_id: 342157 cord_uid: qjyooq68 file: cache/cord-355610-7xy4s483.json key: cord-355610-7xy4s483 authors: Hu, Dan; Zhu, Zhongyu; Li, Shun; Deng, Yongqiang; Wu, Yanling; Zhang, Nana; Puri, Vinita; Wang, Chunyu; Zou, Peng; Lei, Cheng; Tian, Xiaolong; Wang, Yulu; Zhao, Qi; Li, Wei; Prabakaran, Ponraj; Feng, Yang; Cardosa, Jane; Qin, Chengfeng; Zhou, Xiaohui; Dimitrov, Dimiter S.; Ying, Tianlei title: A broadly neutralizing germline-like human monoclonal antibody against dengue virus envelope domain III date: 2019-06-26 journal: PLoS Pathog DOI: 10.1371/journal.ppat.1007836 sha: doc_id: 355610 cord_uid: 7xy4s483 file: cache/cord-355906-yeaw9nr8.json key: cord-355906-yeaw9nr8 authors: Nedjadi, Taoufik; El-Kafrawy, Sherif; Sohrab, Sayed S.; Desprès, Philippe; Damanhouri, Ghazi; Azhar, Esam title: Tackling dengue fever: Current status and challenges date: 2015-12-09 journal: Virol J DOI: 10.1186/s12985-015-0444-8 sha: doc_id: 355906 cord_uid: yeaw9nr8 file: cache/cord-303818-z3js3mr4.json key: cord-303818-z3js3mr4 authors: Chen, Huixin; Parimelalagan, Mariya; Lai, Yee Ling; Lee, Kim Sung; Koay, Evelyn Siew-Chuan; Hapuarachchi, Hapuarachchige C.; Ng, Lee Ching; Ho, Phui San; Chu, Justin Jang Hann title: Development and Evaluation of a SYBR Green–Based Real-Time Multiplex RT-PCR Assay for Simultaneous Detection and Serotyping of Dengue and Chikungunya Viruses date: 2015-10-11 journal: J Mol Diagn DOI: 10.1016/j.jmoldx.2015.06.008 sha: doc_id: 303818 cord_uid: z3js3mr4 file: cache/cord-344020-8poerd09.json key: cord-344020-8poerd09 authors: Vermeulen, Tom D; Reimerink, Johan; Reusken, Chantal; Giron, Sandra; de Vries, Peter J title: Autochthonous dengue in two Dutch tourists visiting Département Var, southern France, July 2020 date: 2020-10-01 journal: Euro Surveill DOI: 10.2807/1560-7917.es.2020.25.39.2001670 sha: doc_id: 344020 cord_uid: 8poerd09 file: cache/cord-319174-pbqjg7hf.json key: cord-319174-pbqjg7hf authors: Song, Ke-Yu; Zhao, Hui; Jiang, Zhen-You; Li, Xiao-Feng; Deng, Yong-Qiang; Jiang, Tao; Zhu, Shun-Ya; Shi, Pei-Yong; Zhang, Bo; Zhang, Fu-Chun; Qin, E-De; Qin, Cheng-Feng title: A novel reporter system for neutralizing and enhancing antibody assay against dengue virus date: 2014-02-18 journal: BMC Microbiol DOI: 10.1186/1471-2180-14-44 sha: doc_id: 319174 cord_uid: pbqjg7hf file: cache/cord-282204-j1slaefb.json key: cord-282204-j1slaefb authors: Silva, José V.J.; Ludwig-Begall, Louisa F.; Oliveira-Filho, Edmilson F. de; Oliveira, Renato A.S.; Durães-Carvalho, Ricardo; Lopes, Thaísa R.R.; Silva, Daisy E.A.; Gil, Laura H.V.G. title: A scoping review of Chikungunya virus infection: epidemiology, clinical characteristics, viral co-circulation complications, and control date: 2018-12-31 journal: Acta Tropica DOI: 10.1016/j.actatropica.2018.09.003 sha: doc_id: 282204 cord_uid: j1slaefb file: cache/cord-273065-peqz7okh.json key: cord-273065-peqz7okh authors: Girard, Marc; Nelson, Christopher B.; Picot, Valentina; Gubler, Duane J. title: Arboviruses: A global public health threat date: 2020-04-24 journal: Vaccine DOI: 10.1016/j.vaccine.2020.04.011 sha: doc_id: 273065 cord_uid: peqz7okh file: cache/cord-355179-wmfwl2bh.json key: cord-355179-wmfwl2bh authors: Jung, Eunhye; Nam, Sangwoo; Oh, Hyeryeon; Jun, Sangmi; Ro, Hyun-Joo; Kim, Baek; Kim, Meehyein; Go, Yun Young title: Neutralization of Acidic Intracellular Vesicles by Niclosamide Inhibits Multiple Steps of the Dengue Virus Life Cycle In Vitro date: 2019-06-18 journal: Sci Rep DOI: 10.1038/s41598-019-45095-1 sha: doc_id: 355179 cord_uid: wmfwl2bh Reading metadata file and updating bibliogrpahics === updating bibliographic database Building study carrel named keyword-denv-cord === file2bib.sh === OMP: Error #34: System unable to allocate necessary resources for OMP thread: OMP: System error #11: Resource temporarily unavailable OMP: Hint Try decreasing the value of OMP_NUM_THREADS. /data-disk/reader-compute/reader-cord/bin/file2bib.sh: line 39: 20805 Aborted $FILE2BIB "$FILE" > "$OUTPUT" === file2bib.sh === OMP: Error #34: System unable to allocate necessary resources for OMP thread: OMP: System error #11: Resource temporarily unavailable OMP: Hint Try decreasing the value of OMP_NUM_THREADS. /data-disk/reader-compute/reader-cord/bin/file2bib.sh: line 39: 19513 Aborted $FILE2BIB "$FILE" > "$OUTPUT" === file2bib.sh === OMP: Error #34: System unable to allocate necessary resources for OMP thread: OMP: System error #11: Resource temporarily unavailable OMP: Hint Try decreasing the value of OMP_NUM_THREADS. /data-disk/reader-compute/reader-cord/bin/file2bib.sh: line 39: 19460 Aborted $FILE2BIB "$FILE" > "$OUTPUT" === file2bib.sh === OMP: Error #34: System unable to allocate necessary resources for OMP thread: OMP: System error #11: Resource temporarily unavailable OMP: Hint Try decreasing the value of OMP_NUM_THREADS. /data-disk/reader-compute/reader-cord/bin/file2bib.sh: line 39: 19598 Aborted $FILE2BIB "$FILE" > "$OUTPUT" === file2bib.sh === OMP: Error #34: System unable to allocate necessary resources for OMP thread: OMP: System error #11: Resource temporarily unavailable OMP: Hint Try decreasing the value of OMP_NUM_THREADS. /data-disk/reader-compute/reader-cord/bin/file2bib.sh: line 39: 23100 Aborted $FILE2BIB "$FILE" > "$OUTPUT" === file2bib.sh === OMP: Error #34: System unable to allocate necessary resources for OMP thread: OMP: System error #11: Resource temporarily unavailable OMP: Hint Try decreasing the value of OMP_NUM_THREADS. /data-disk/reader-compute/reader-cord/bin/file2bib.sh: line 39: 19801 Aborted $FILE2BIB "$FILE" > "$OUTPUT" === file2bib.sh === OMP: Error #34: System unable to allocate necessary resources for OMP thread: OMP: System error #11: Resource temporarily unavailable OMP: Hint Try decreasing the value of OMP_NUM_THREADS. /data-disk/reader-compute/reader-cord/bin/file2bib.sh: line 39: 20479 Aborted $FILE2BIB "$FILE" > "$OUTPUT" === file2bib.sh === OMP: Error #34: System unable to allocate necessary resources for OMP thread: OMP: System error #11: Resource temporarily unavailable OMP: Hint Try decreasing the value of OMP_NUM_THREADS. /data-disk/reader-compute/reader-cord/bin/file2bib.sh: line 39: 20535 Aborted $FILE2BIB "$FILE" > "$OUTPUT" === file2bib.sh === OMP: Error #34: System unable to allocate necessary resources for OMP thread: OMP: System error #11: Resource temporarily unavailable OMP: Hint Try decreasing the value of OMP_NUM_THREADS. /data-disk/reader-compute/reader-cord/bin/file2bib.sh: line 39: 20623 Aborted $FILE2BIB "$FILE" > "$OUTPUT" === file2bib.sh === OMP: Error #34: System unable to allocate necessary resources for OMP thread: OMP: System error #11: Resource temporarily unavailable OMP: Hint Try decreasing the value of OMP_NUM_THREADS. /data-disk/reader-compute/reader-cord/bin/file2bib.sh: line 39: 21212 Aborted $FILE2BIB "$FILE" > "$OUTPUT" === file2bib.sh === OMP: Error #34: System unable to allocate necessary resources for OMP thread: OMP: System error #11: Resource temporarily unavailable OMP: Hint Try decreasing the value of OMP_NUM_THREADS. /data-disk/reader-compute/reader-cord/bin/file2bib.sh: line 39: 22645 Aborted $FILE2BIB "$FILE" > "$OUTPUT" === file2bib.sh === OMP: Error #34: System unable to allocate necessary resources for OMP thread: OMP: System error #11: Resource temporarily unavailable OMP: Hint Try decreasing the value of OMP_NUM_THREADS. /data-disk/reader-compute/reader-cord/bin/file2bib.sh: line 39: 22649 Aborted $FILE2BIB "$FILE" > "$OUTPUT" === file2bib.sh === OMP: Error #34: System unable to allocate necessary resources for OMP thread: OMP: System error #11: Resource temporarily unavailable OMP: Hint Try decreasing the value of OMP_NUM_THREADS. /data-disk/reader-compute/reader-cord/bin/file2bib.sh: line 39: 21559 Aborted $FILE2BIB "$FILE" > "$OUTPUT" === file2bib.sh === OMP: Error #34: System unable to allocate necessary resources for OMP thread: OMP: System error #11: Resource temporarily unavailable OMP: Hint Try decreasing the value of OMP_NUM_THREADS. /data-disk/reader-compute/reader-cord/bin/file2bib.sh: line 39: 22515 Aborted $FILE2BIB "$FILE" > "$OUTPUT" === file2bib.sh === OMP: Error #34: System unable to allocate necessary resources for OMP thread: OMP: System error #11: Resource temporarily unavailable OMP: Hint Try decreasing the value of OMP_NUM_THREADS. /data-disk/reader-compute/reader-cord/bin/file2bib.sh: line 39: 21390 Aborted $FILE2BIB "$FILE" > "$OUTPUT" === file2bib.sh === OMP: Error #34: System unable to allocate necessary resources for OMP thread: OMP: System error #11: Resource temporarily unavailable OMP: Hint Try decreasing the value of OMP_NUM_THREADS. /data-disk/reader-compute/reader-cord/bin/file2bib.sh: line 39: 23138 Aborted $FILE2BIB "$FILE" > "$OUTPUT" === file2bib.sh === OMP: Error #34: System unable to allocate necessary resources for OMP thread: OMP: System error #11: Resource temporarily unavailable OMP: Hint Try decreasing the value of OMP_NUM_THREADS. /data-disk/reader-compute/reader-cord/bin/file2bib.sh: line 39: 21578 Aborted $FILE2BIB "$FILE" > "$OUTPUT" === file2bib.sh === id: cord-002085-e7xwb03g author: Yamashita, Akifumi title: DGV: Dengue Genographic Viewer date: 2016-06-07 pages: extension: .txt txt: ./txt/cord-002085-e7xwb03g.txt cache: ./cache/cord-002085-e7xwb03g.txt Content-Encoding UTF-8 Content-Type text/plain; charset=UTF-8 X-Parsed-By ['org.apache.tika.parser.DefaultParser', 'org.apache.tika.parser.csv.TextAndCSVParser'] X-TIKA:content_handler ToTextContentHandler X-TIKA:embedded_depth 0 X-TIKA:parse_time_millis 3 resourceName b'cord-002085-e7xwb03g.txt' === file2bib.sh === id: cord-270495-2u072mtp author: Lokida, Dewi title: Diagnosis of COVID-19 in a Dengue-Endemic Area date: 2020-08-05 pages: extension: .txt txt: ./txt/cord-270495-2u072mtp.txt cache: ./cache/cord-270495-2u072mtp.txt Content-Encoding UTF-8 Content-Type text/plain; charset=UTF-8 X-Parsed-By ['org.apache.tika.parser.DefaultParser', 'org.apache.tika.parser.csv.TextAndCSVParser'] X-TIKA:content_handler ToTextContentHandler X-TIKA:embedded_depth 0 X-TIKA:parse_time_millis 2 resourceName b'cord-270495-2u072mtp.txt' === file2bib.sh === id: cord-272459-w14finxf author: Heaton, Nicholas S. title: Dengue Virus and Autophagy date: 2011-08-04 pages: extension: .txt txt: ./txt/cord-272459-w14finxf.txt cache: ./cache/cord-272459-w14finxf.txt Content-Encoding UTF-8 Content-Type text/plain; charset=UTF-8 X-Parsed-By ['org.apache.tika.parser.DefaultParser', 'org.apache.tika.parser.csv.TextAndCSVParser'] X-TIKA:content_handler ToTextContentHandler X-TIKA:embedded_depth 0 X-TIKA:parse_time_millis 3 resourceName b'cord-272459-w14finxf.txt' === file2bib.sh === id: cord-278260-3o91v72a author: Halstead, Scott B title: COVID 19 Vaccines: Should we fear ADE? date: 2020-08-12 pages: extension: .txt txt: ./txt/cord-278260-3o91v72a.txt cache: ./cache/cord-278260-3o91v72a.txt Content-Encoding ISO-8859-1 Content-Type text/plain; charset=ISO-8859-1 X-Parsed-By ['org.apache.tika.parser.DefaultParser', 'org.apache.tika.parser.csv.TextAndCSVParser'] X-TIKA:content_handler ToTextContentHandler X-TIKA:embedded_depth 0 X-TIKA:parse_time_millis 2 resourceName b'cord-278260-3o91v72a.txt' === file2bib.sh === id: cord-307219-okvvajms author: Lazzarini, Luca title: First autochthonous dengue outbreak in Italy, August 2020 date: 2020-09-10 pages: extension: .txt txt: ./txt/cord-307219-okvvajms.txt cache: ./cache/cord-307219-okvvajms.txt Content-Encoding UTF-8 Content-Type text/plain; charset=UTF-8 X-Parsed-By ['org.apache.tika.parser.DefaultParser', 'org.apache.tika.parser.csv.TextAndCSVParser'] X-TIKA:content_handler ToTextContentHandler X-TIKA:embedded_depth 0 X-TIKA:parse_time_millis 3 resourceName b'cord-307219-okvvajms.txt' === file2bib.sh === id: cord-253480-qchrw337 author: Pu, Jieying title: Antiviral activity of Carbenoxolone disodium against dengue virus infection date: 2016-12-23 pages: extension: .txt txt: ./txt/cord-253480-qchrw337.txt cache: ./cache/cord-253480-qchrw337.txt Content-Encoding UTF-8 Content-Type text/plain; charset=UTF-8 X-Parsed-By ['org.apache.tika.parser.DefaultParser', 'org.apache.tika.parser.csv.TextAndCSVParser'] X-TIKA:content_handler ToTextContentHandler X-TIKA:embedded_depth 0 X-TIKA:parse_time_millis 3 resourceName b'cord-253480-qchrw337.txt' === file2bib.sh === id: cord-002426-5e1xn7kj author: Falcón-Lezama, Jorge Abelardo title: Analysis of spatial mobility in subjects from a Dengue endemic urban locality in Morelos State, Mexico date: 2017-02-22 pages: extension: .txt txt: ./txt/cord-002426-5e1xn7kj.txt cache: ./cache/cord-002426-5e1xn7kj.txt Content-Encoding UTF-8 Content-Type text/plain; charset=UTF-8 X-Parsed-By ['org.apache.tika.parser.DefaultParser', 'org.apache.tika.parser.csv.TextAndCSVParser'] X-TIKA:content_handler ToTextContentHandler X-TIKA:embedded_depth 0 X-TIKA:parse_time_millis 3 resourceName b'cord-002426-5e1xn7kj.txt' === file2bib.sh === id: cord-344020-8poerd09 author: Vermeulen, Tom D title: Autochthonous dengue in two Dutch tourists visiting Département Var, southern France, July 2020 date: 2020-10-01 pages: extension: .txt txt: ./txt/cord-344020-8poerd09.txt cache: ./cache/cord-344020-8poerd09.txt Content-Encoding UTF-8 Content-Type text/plain; charset=UTF-8 X-Parsed-By ['org.apache.tika.parser.DefaultParser', 'org.apache.tika.parser.csv.TextAndCSVParser'] X-TIKA:content_handler ToTextContentHandler X-TIKA:embedded_depth 0 X-TIKA:parse_time_millis 3 resourceName b'cord-344020-8poerd09.txt' === file2bib.sh === id: cord-277547-2vim1wno author: Zandi, Keivan title: Antiviral activity of four types of bioflavonoid against dengue virus type-2 date: 2011-12-28 pages: extension: .txt txt: ./txt/cord-277547-2vim1wno.txt cache: ./cache/cord-277547-2vim1wno.txt Content-Encoding UTF-8 Content-Type text/plain; charset=UTF-8 X-Parsed-By ['org.apache.tika.parser.DefaultParser', 'org.apache.tika.parser.csv.TextAndCSVParser'] X-TIKA:content_handler ToTextContentHandler X-TIKA:embedded_depth 0 X-TIKA:parse_time_millis 3 resourceName b'cord-277547-2vim1wno.txt' === file2bib.sh === id: cord-011390-98xyie7s author: Darcy, Andrew Waleluma title: Multiple arboviral infections during a DENV-2 outbreak in Solomon Islands date: 2020-05-15 pages: extension: .txt txt: ./txt/cord-011390-98xyie7s.txt cache: ./cache/cord-011390-98xyie7s.txt Content-Encoding UTF-8 Content-Type text/plain; charset=UTF-8 X-Parsed-By ['org.apache.tika.parser.DefaultParser', 'org.apache.tika.parser.csv.TextAndCSVParser'] X-TIKA:content_handler ToTextContentHandler X-TIKA:embedded_depth 0 X-TIKA:parse_time_millis 3 resourceName b'cord-011390-98xyie7s.txt' === file2bib.sh === id: cord-355489-tkvfneje author: Mendez, Jairo A title: Phylogenetic history demonstrates two different lineages of dengue type 1 virus in Colombia date: 2010-09-14 pages: extension: .txt txt: ./txt/cord-355489-tkvfneje.txt cache: ./cache/cord-355489-tkvfneje.txt Content-Encoding UTF-8 Content-Type text/plain; charset=UTF-8 X-Parsed-By ['org.apache.tika.parser.DefaultParser', 'org.apache.tika.parser.csv.TextAndCSVParser'] X-TIKA:content_handler ToTextContentHandler X-TIKA:embedded_depth 0 X-TIKA:parse_time_millis 3 resourceName b'cord-355489-tkvfneje.txt' === file2bib.sh === id: cord-317169-qlqavi4t author: Chiow, K.H. title: Evaluation of antiviral activities of Houttuynia cordata Thunb. extract, quercetin, quercetrin and cinanserin on murine coronavirus and dengue virus infection date: 2015-12-19 pages: extension: .txt txt: ./txt/cord-317169-qlqavi4t.txt cache: ./cache/cord-317169-qlqavi4t.txt Content-Encoding UTF-8 Content-Type text/plain; charset=UTF-8 X-Parsed-By ['org.apache.tika.parser.DefaultParser', 'org.apache.tika.parser.csv.TextAndCSVParser'] X-TIKA:content_handler ToTextContentHandler X-TIKA:embedded_depth 0 X-TIKA:parse_time_millis 4 resourceName b'cord-317169-qlqavi4t.txt' === file2bib.sh === id: cord-011026-iapgkz0p author: El-Bitar, Alaa M. H. title: Smp76, a Scorpine-Like Peptide Isolated from the Venom of the Scorpion Scorpio maurus palmatus, with a Potent Antiviral Activity Against Hepatitis C Virus and Dengue Virus date: 2019-07-06 pages: extension: .txt txt: ./txt/cord-011026-iapgkz0p.txt cache: ./cache/cord-011026-iapgkz0p.txt Content-Encoding UTF-8 Content-Type text/plain; charset=UTF-8 X-Parsed-By ['org.apache.tika.parser.DefaultParser', 'org.apache.tika.parser.csv.TextAndCSVParser'] X-TIKA:content_handler ToTextContentHandler X-TIKA:embedded_depth 0 X-TIKA:parse_time_millis 3 resourceName b'cord-011026-iapgkz0p.txt' === file2bib.sh === id: cord-318120-vfznyyz6 author: Dauner, Allison L. title: Development of a pan-serotype reverse transcription loop-mediated isothermal amplification assay for the detection of dengue virus date: 2015-05-15 pages: extension: .txt txt: ./txt/cord-318120-vfznyyz6.txt cache: ./cache/cord-318120-vfznyyz6.txt Content-Encoding UTF-8 Content-Type text/plain; charset=UTF-8 X-Parsed-By ['org.apache.tika.parser.DefaultParser', 'org.apache.tika.parser.csv.TextAndCSVParser'] X-TIKA:content_handler ToTextContentHandler X-TIKA:embedded_depth 0 X-TIKA:parse_time_millis 3 resourceName b'cord-318120-vfznyyz6.txt' === file2bib.sh === id: cord-282742-eyukbot7 author: Diosa-Toro, Mayra title: Arthropod-Borne Flaviviruses and RNA Interference: Seeking New Approaches for Antiviral Therapy date: 2013-02-20 pages: extension: .txt txt: ./txt/cord-282742-eyukbot7.txt cache: ./cache/cord-282742-eyukbot7.txt Content-Encoding ISO-8859-1 Content-Type text/plain; charset=ISO-8859-1 X-Parsed-By ['org.apache.tika.parser.DefaultParser', 'org.apache.tika.parser.csv.TextAndCSVParser'] X-TIKA:content_handler ToTextContentHandler X-TIKA:embedded_depth 0 X-TIKA:parse_time_millis 3 resourceName b'cord-282742-eyukbot7.txt' === file2bib.sh === id: cord-002413-795wuqz5 author: Balinsky, Corey A. title: IRAV (FLJ11286), an Interferon-Stimulated Gene with Antiviral Activity against Dengue Virus, Interacts with MOV10 date: 2017-02-14 pages: extension: .txt txt: ./txt/cord-002413-795wuqz5.txt cache: ./cache/cord-002413-795wuqz5.txt Content-Encoding UTF-8 Content-Type text/plain; charset=UTF-8 X-Parsed-By ['org.apache.tika.parser.DefaultParser', 'org.apache.tika.parser.csv.TextAndCSVParser'] X-TIKA:content_handler ToTextContentHandler X-TIKA:embedded_depth 0 X-TIKA:parse_time_millis 4 resourceName b'cord-002413-795wuqz5.txt' === file2bib.sh === id: cord-330743-o11d0aa1 author: Yu, Xi title: Broad-spectrum virucidal activity of bacterial secreted lipases against flaviviruses, SARS-CoV-2 and other enveloped viruses date: 2020-05-25 pages: extension: .txt txt: ./txt/cord-330743-o11d0aa1.txt cache: ./cache/cord-330743-o11d0aa1.txt Content-Encoding UTF-8 Content-Type text/plain; charset=UTF-8 X-Parsed-By ['org.apache.tika.parser.DefaultParser', 'org.apache.tika.parser.csv.TextAndCSVParser'] X-TIKA:content_handler ToTextContentHandler X-TIKA:embedded_depth 0 X-TIKA:parse_time_millis 4 resourceName b'cord-330743-o11d0aa1.txt' === file2bib.sh === id: cord-353241-ityhcak7 author: Zhu, Hanliang title: IoT PCR for pandemic disease detection and its spread monitoring date: 2020-01-15 pages: extension: .txt txt: ./txt/cord-353241-ityhcak7.txt cache: ./cache/cord-353241-ityhcak7.txt Content-Encoding UTF-8 Content-Type text/plain; charset=UTF-8 X-Parsed-By ['org.apache.tika.parser.DefaultParser', 'org.apache.tika.parser.csv.TextAndCSVParser'] X-TIKA:content_handler ToTextContentHandler X-TIKA:embedded_depth 0 X-TIKA:parse_time_millis 3 resourceName b'cord-353241-ityhcak7.txt' === file2bib.sh === id: cord-318614-518giv0m author: Tsai, Jih-Jin title: A fully automated sample-to-answer PCR system for easy and sensitive detection of dengue virus in human serum and mosquitos date: 2019-07-10 pages: extension: .txt txt: ./txt/cord-318614-518giv0m.txt cache: ./cache/cord-318614-518giv0m.txt Content-Encoding UTF-8 Content-Type text/plain; charset=UTF-8 X-Parsed-By ['org.apache.tika.parser.DefaultParser', 'org.apache.tika.parser.csv.TextAndCSVParser'] X-TIKA:content_handler ToTextContentHandler X-TIKA:embedded_depth 0 X-TIKA:parse_time_millis 3 resourceName b'cord-318614-518giv0m.txt' === file2bib.sh === id: cord-286255-ded5t1ai author: Tomashek, Kay M. title: Clinical and epidemiologic characteristics of dengue and other etiologic agents among patients with acute febrile illness, Puerto Rico, 2012–2015 date: 2017-09-13 pages: extension: .txt txt: ./txt/cord-286255-ded5t1ai.txt cache: ./cache/cord-286255-ded5t1ai.txt Content-Encoding UTF-8 Content-Type text/plain; charset=UTF-8 X-Parsed-By ['org.apache.tika.parser.DefaultParser', 'org.apache.tika.parser.csv.TextAndCSVParser'] X-TIKA:content_handler ToTextContentHandler X-TIKA:embedded_depth 0 X-TIKA:parse_time_millis 3 resourceName b'cord-286255-ded5t1ai.txt' === file2bib.sh === id: cord-276718-3lujp0oy author: Neeraja, M. title: Rapid detection and differentiation of dengue virus serotypes by NS1 specific reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay in patients presenting to a tertiary care hospital in Hyderabad, India date: 2014-10-24 pages: extension: .txt txt: ./txt/cord-276718-3lujp0oy.txt cache: ./cache/cord-276718-3lujp0oy.txt Content-Encoding UTF-8 Content-Type text/plain; charset=UTF-8 X-Parsed-By ['org.apache.tika.parser.DefaultParser', 'org.apache.tika.parser.csv.TextAndCSVParser'] X-TIKA:content_handler ToTextContentHandler X-TIKA:embedded_depth 0 X-TIKA:parse_time_millis 4 resourceName b'cord-276718-3lujp0oy.txt' === file2bib.sh === id: cord-306204-rkher4ly author: Pong, Lian Yih title: Anti-dengue virus serotype 2 activity of tannins from porcupine dates date: 2020-05-20 pages: extension: .txt txt: ./txt/cord-306204-rkher4ly.txt cache: ./cache/cord-306204-rkher4ly.txt Content-Encoding UTF-8 Content-Type text/plain; charset=UTF-8 X-Parsed-By ['org.apache.tika.parser.DefaultParser', 'org.apache.tika.parser.csv.TextAndCSVParser'] X-TIKA:content_handler ToTextContentHandler X-TIKA:embedded_depth 0 X-TIKA:parse_time_millis 3 resourceName b'cord-306204-rkher4ly.txt' === file2bib.sh === id: cord-355179-wmfwl2bh author: Jung, Eunhye title: Neutralization of Acidic Intracellular Vesicles by Niclosamide Inhibits Multiple Steps of the Dengue Virus Life Cycle In Vitro date: 2019-06-18 pages: extension: .txt txt: ./txt/cord-355179-wmfwl2bh.txt cache: ./cache/cord-355179-wmfwl2bh.txt Content-Encoding UTF-8 Content-Type text/plain; charset=UTF-8 X-Parsed-By ['org.apache.tika.parser.DefaultParser', 'org.apache.tika.parser.csv.TextAndCSVParser'] X-TIKA:content_handler ToTextContentHandler X-TIKA:embedded_depth 0 X-TIKA:parse_time_millis 3 resourceName b'cord-355179-wmfwl2bh.txt' === file2bib.sh === id: cord-328661-spxgox52 author: Yu, Jianhai title: Epidemiological and Evolutionary Analysis of Dengue-1 Virus Detected in Guangdong during 2014: Recycling of Old and Formation of New Lineages date: 2019-08-05 pages: extension: .txt txt: ./txt/cord-328661-spxgox52.txt cache: ./cache/cord-328661-spxgox52.txt Content-Encoding UTF-8 Content-Type text/plain; charset=UTF-8 X-Parsed-By ['org.apache.tika.parser.DefaultParser', 'org.apache.tika.parser.csv.TextAndCSVParser'] X-TIKA:content_handler ToTextContentHandler X-TIKA:embedded_depth 0 X-TIKA:parse_time_millis 4 resourceName b'cord-328661-spxgox52.txt' === file2bib.sh === id: cord-011968-abd891ej author: Lai, Yen-Chung title: Roles of Macrophage Migration Inhibitory Factor in Dengue Pathogenesis: From Pathogenic Factor to Therapeutic Target date: 2020-06-12 pages: extension: .txt txt: ./txt/cord-011968-abd891ej.txt cache: ./cache/cord-011968-abd891ej.txt Content-Encoding UTF-8 Content-Type text/plain; charset=UTF-8 X-Parsed-By ['org.apache.tika.parser.DefaultParser', 'org.apache.tika.parser.csv.TextAndCSVParser'] X-TIKA:content_handler ToTextContentHandler X-TIKA:embedded_depth 0 X-TIKA:parse_time_millis 3 resourceName b'cord-011968-abd891ej.txt' === file2bib.sh === id: cord-276364-zyw5aukk author: Wong, Ho Him title: Manipulation of autophagy by (+) RNA viruses date: 2019-08-08 pages: extension: .txt txt: ./txt/cord-276364-zyw5aukk.txt cache: ./cache/cord-276364-zyw5aukk.txt Content-Encoding UTF-8 Content-Type text/plain; charset=UTF-8 X-Parsed-By ['org.apache.tika.parser.DefaultParser', 'org.apache.tika.parser.csv.TextAndCSVParser'] X-TIKA:content_handler ToTextContentHandler X-TIKA:embedded_depth 0 X-TIKA:parse_time_millis 3 resourceName b'cord-276364-zyw5aukk.txt' === file2bib.sh === id: cord-342157-qjyooq68 author: King, Chwan-Chuen title: Comparative analysis of full genomic sequences among different genotypes of dengue virus type 3 date: 2008-05-21 pages: extension: .txt txt: ./txt/cord-342157-qjyooq68.txt cache: ./cache/cord-342157-qjyooq68.txt Content-Encoding UTF-8 Content-Type text/plain; charset=UTF-8 X-Parsed-By ['org.apache.tika.parser.DefaultParser', 'org.apache.tika.parser.csv.TextAndCSVParser'] X-TIKA:content_handler ToTextContentHandler X-TIKA:embedded_depth 0 X-TIKA:parse_time_millis 2 resourceName b'cord-342157-qjyooq68.txt' === file2bib.sh === id: cord-002179-v8lpw4r7 author: Viktorovskaya, Olga V. title: Identification of RNA Binding Proteins Associated with Dengue Virus RNA in Infected Cells Reveals Temporally Distinct Host Factor Requirements date: 2016-08-24 pages: extension: .txt txt: ./txt/cord-002179-v8lpw4r7.txt cache: ./cache/cord-002179-v8lpw4r7.txt Content-Encoding UTF-8 Content-Type text/plain; charset=UTF-8 X-Parsed-By ['org.apache.tika.parser.DefaultParser', 'org.apache.tika.parser.csv.TextAndCSVParser'] X-TIKA:content_handler ToTextContentHandler X-TIKA:embedded_depth 0 X-TIKA:parse_time_millis 3 resourceName b'cord-002179-v8lpw4r7.txt' === file2bib.sh === id: cord-345898-a6vt8kso author: Ren, Linzhu title: Live Cell Reporter Systems for Positive-Sense Single Strand RNA Viruses date: 2016-01-04 pages: extension: .txt txt: ./txt/cord-345898-a6vt8kso.txt cache: ./cache/cord-345898-a6vt8kso.txt Content-Encoding UTF-8 Content-Type text/plain; charset=UTF-8 X-Parsed-By ['org.apache.tika.parser.DefaultParser', 'org.apache.tika.parser.csv.TextAndCSVParser'] X-TIKA:content_handler ToTextContentHandler X-TIKA:embedded_depth 0 X-TIKA:parse_time_millis 5 resourceName b'cord-345898-a6vt8kso.txt' === file2bib.sh === id: cord-285538-3ah05ijf author: Halstead, Scott B. title: Chapter 19 Pathogenic Exploitation of Fc Activity date: 2014-12-31 pages: extension: .txt txt: ./txt/cord-285538-3ah05ijf.txt cache: ./cache/cord-285538-3ah05ijf.txt Content-Encoding UTF-8 Content-Type text/plain; charset=UTF-8 X-Parsed-By ['org.apache.tika.parser.DefaultParser', 'org.apache.tika.parser.csv.TextAndCSVParser'] X-TIKA:content_handler ToTextContentHandler X-TIKA:embedded_depth 0 X-TIKA:parse_time_millis 4 resourceName b'cord-285538-3ah05ijf.txt' === file2bib.sh === id: cord-256732-md1u51va author: Shrivastava, Gaurav title: Inflammasome Fuels Dengue Severity date: 2020-09-10 pages: extension: .txt txt: ./txt/cord-256732-md1u51va.txt cache: ./cache/cord-256732-md1u51va.txt Content-Encoding UTF-8 Content-Type text/plain; charset=UTF-8 X-Parsed-By ['org.apache.tika.parser.DefaultParser', 'org.apache.tika.parser.csv.TextAndCSVParser'] X-TIKA:content_handler ToTextContentHandler X-TIKA:embedded_depth 0 X-TIKA:parse_time_millis 3 resourceName b'cord-256732-md1u51va.txt' === file2bib.sh === id: cord-282204-j1slaefb author: Silva, José V.J. title: A scoping review of Chikungunya virus infection: epidemiology, clinical characteristics, viral co-circulation complications, and control date: 2018-12-31 pages: extension: .txt txt: ./txt/cord-282204-j1slaefb.txt cache: ./cache/cord-282204-j1slaefb.txt Content-Encoding UTF-8 Content-Type text/plain; charset=UTF-8 X-Parsed-By ['org.apache.tika.parser.DefaultParser', 'org.apache.tika.parser.csv.TextAndCSVParser'] X-TIKA:content_handler ToTextContentHandler X-TIKA:embedded_depth 0 X-TIKA:parse_time_millis 5 resourceName b'cord-282204-j1slaefb.txt' === file2bib.sh === id: cord-297662-slmlhqnb author: Yap, Sally S. L. title: Dengue Virus Glycosylation: What Do We Know? date: 2017-07-25 pages: extension: .txt txt: ./txt/cord-297662-slmlhqnb.txt cache: ./cache/cord-297662-slmlhqnb.txt Content-Encoding UTF-8 Content-Type text/plain; charset=UTF-8 X-Parsed-By ['org.apache.tika.parser.DefaultParser', 'org.apache.tika.parser.csv.TextAndCSVParser'] X-TIKA:content_handler ToTextContentHandler X-TIKA:embedded_depth 0 X-TIKA:parse_time_millis 4 resourceName b'cord-297662-slmlhqnb.txt' Que is empty; done keyword-denv-cord === reduce.pl bib === id = cord-002085-e7xwb03g author = Yamashita, Akifumi title = DGV: Dengue Genographic Viewer date = 2016-06-07 pages = extension = .txt mime = text/plain words = 2456 sentences = 130 flesch = 48 summary = We constructed a DENV database containing the serotype, genotype, year and country/region of collection by collecting all publically available DENV sequence information from the National Center for Biotechnology Information (NCBI) and assigning genotype information. DGV also assigns the serotype and genotype to a user-specified sequence by performing a homology search against the curated DENV database, and shows its homologous sequences with the geographical position and year of collection. The second database is the Dengue virus genotyping database 2 (Yamashita et al., 2013) , which provides a summary table containing the DENV serotype/genotype, year and country of collection and accession number. The Dengue Virus Resource 3 facilitates the retrieval of DENV sequences deposited in GenBank according to serotype, disease symptom, host, region/country, genome region, and collection and/or release data (Resch et al., 2009) . DGV provides a search engine for the assignment of the DENV serotype, genotype, and origin country according to the most homologous sequence on the basis of a blastn search against the DENV database. cache = ./cache/cord-002085-e7xwb03g.txt txt = ./txt/cord-002085-e7xwb03g.txt === reduce.pl bib === id = cord-002413-795wuqz5 author = Balinsky, Corey A. title = IRAV (FLJ11286), an Interferon-Stimulated Gene with Antiviral Activity against Dengue Virus, Interacts with MOV10 date = 2017-02-14 pages = extension = .txt mime = text/plain words = 5485 sentences = 278 flesch = 47 summary = title: IRAV (FLJ11286), an Interferon-Stimulated Gene with Antiviral Activity against Dengue Virus, Interacts with MOV10 IRAV is an RNA binding protein and localizes to cytoplasmic processing bodies (P bodies) in uninfected cells, where it interacts with the MOV10 RISC complex RNA helicase, suggesting a role for IRAV in the processing of viral RNA. FLJ11286, which we refer to here as IRAV (interferon-regulated antiviral gene) (also annotated as C19orf66, UPF0515, or RyDEN), encodes a protein 291 amino acids (aa) in length with a calculated molecular mass of 33.1 kDa. Analysis of published microarray data suggests that IRAV (FLJ11286) is upregulated in response to type I and type II IFNs (6, 20, (24) (25) (26) . IRAV also associates with the host RNA binding proteins UPF1 and HuR (ELAV1) and interacts with MOV10 (a RISC complex RNA helicase), suggesting a role for IRAV in processing or stability of RNA. cache = ./cache/cord-002413-795wuqz5.txt txt = ./txt/cord-002413-795wuqz5.txt === reduce.pl bib === id = cord-276364-zyw5aukk author = Wong, Ho Him title = Manipulation of autophagy by (+) RNA viruses date = 2019-08-08 pages = extension = .txt mime = text/plain words = 6884 sentences = 360 flesch = 33 summary = Over the past few decades, a growing body of research has defined the critical role of this pathway in facilitating infection by numerous +RNA RNA viruses, including poliovirus (PV) [7, 8] , Coxsackievirus B3 (CVB3) [9, 10] , CVB4 [11] , Enterovirus 71 (EV71) [12] , Human rhinovirus (HRV) [13] , Foot-and-mouth disease virus (FMDV) [14] , encephalomyocarditis virus (EMCV) [15] , Dengue virus (DENV) [16, 17] , Zika virus (ZIKV) [18, 19] , Hepatitis C virus (HCV) [20] , Mouse hepatitic virus (MHV), Newcastle disease virus (NDV) [21] , Severe and acute respiratory syndrome coronavirus (SARS-CoV) [22] , Chikungunya virus (ChikV) [23] , and Japanese encephalitis virus (JEV) [24] . Delineating the process of viral assembly from replication is technically challenging, especially since both processes would very likely Induces formation of autophagosome-like double-membrane liposomes [112] Summary of Interactions between proteins from positive strand RNA viruses and host autophagy machinery. cache = ./cache/cord-276364-zyw5aukk.txt txt = ./txt/cord-276364-zyw5aukk.txt === reduce.pl bib === id = cord-002426-5e1xn7kj author = Falcón-Lezama, Jorge Abelardo title = Analysis of spatial mobility in subjects from a Dengue endemic urban locality in Morelos State, Mexico date = 2017-02-22 pages = extension = .txt mime = text/plain words = 5944 sentences = 261 flesch = 49 summary = MATERIALS AND METHODS: We carried out a cohort-nested, case-control study with 126 individuals (42 cases, 42 intradomestic controls and 42 population controls) with the goal of describing human mobility patterns of recently Dengue virus-infected subjects, and comparing them with those of non-infected subjects living in an urban endemic locality. CONCLUSIONS: Results of this study show that human mobility in a small urban setting exceeded that considered by local health authority's administrative limits, and was different between recently infected and non-infected subjects living in the same household. These observations provide important insights about the role that human mobility may have in Dengue virus transmission and persistence across endemic geographic areas that need to be taken into account when planning preventive and control measures. Sample: 126 individuals (42 cases, 42 intradomestic controls and 42 population controls) with age older than 12, and residents in Axochiapan, Morelos State, México, were selected from the cohort "Peridomestic infection as determinant for Dengue virus transmission" [13] . cache = ./cache/cord-002426-5e1xn7kj.txt txt = ./txt/cord-002426-5e1xn7kj.txt === reduce.pl bib === === reduce.pl bib === id = cord-253480-qchrw337 author = Pu, Jieying title = Antiviral activity of Carbenoxolone disodium against dengue virus infection date = 2016-12-23 pages = extension = .txt mime = text/plain words = 5289 sentences = 287 flesch = 52 summary = Here, we found that the production of infectious DENV particles was significantly decreased by CBX treatment in DENV‐permissive cells, while the viral RNA and viral protein synthesis were not affected. Moreover, results from time-of-addition study showed that the inhibitory effect of CBX on DENV was exhibited by targeting the virus itself, not the host cells. In this study, we investigated whether CBX treatment inhibits dengue infection by measuring the production of progeny virions and viral RNA as well as viral protein expression. To determine whether CBX inhibits the production of infectious progeny DENV in other dengue-permissive cells, TCID 50 assay was performed to measure the virus titer in CBX-treated THP-1 and HUVEC cells, both of which are widely used in DENV studies [Halstead, 1988; Wu et al., 2000] . CBX treatment did not inhibit DENV-1 or DENV-2 RNA synthesis and protein expression in one replication cycle, but markedly reduced progeny virus The total RNA was isolated from the infected cells and analyzed by quantitative RT-PCR. cache = ./cache/cord-253480-qchrw337.txt txt = ./txt/cord-253480-qchrw337.txt === reduce.pl bib === === reduce.pl bib === === reduce.pl bib === id = cord-278260-3o91v72a author = Halstead, Scott B title = COVID 19 Vaccines: Should we fear ADE? date = 2020-08-12 pages = extension = .txt mime = text/plain words = 2331 sentences = 178 flesch = 44 summary = Within months large numbers of vaccinated children developed a severe breakthrough disease, called "atypical measles." [6] A similar outcome, "vaccine associated enhanced respiratory disease (VAERD)," was observed in infants, 4 -12 months of age, who were given formalininactivated respiratory syncytial virus (RSV) and a few months later infected by RSV. The biological behavior of some coronaviruses in non-human species together with evidence that human coronavirus antibodies enhanced infection of SARS or MERS CoVs in Fc receptor-bearing cells, in vitro, have led to speculations that ADE contributes to disease severity in humans. [11] It has been reported that high levels of SARS CoV-1 IgG antibodies circulated in severe SARS cases and that anti-S IgG neutralizing antibody (NAb) responses developed significantly faster after the onset of clinical symptoms in fatal compared with recovered cases leading some to attribute enhanced tissue damage to ADE. With others, we conclude that the differences in clinical, epidemiological and pathological features of SARS and DENV diseases suggest that iADE does not contribute to the severity of natural human coronavirus infections. cache = ./cache/cord-278260-3o91v72a.txt txt = ./txt/cord-278260-3o91v72a.txt === reduce.pl bib === id = cord-002179-v8lpw4r7 author = Viktorovskaya, Olga V. title = Identification of RNA Binding Proteins Associated with Dengue Virus RNA in Infected Cells Reveals Temporally Distinct Host Factor Requirements date = 2016-08-24 pages = extension = .txt mime = text/plain words = 8021 sentences = 418 flesch = 49 summary = title: Identification of RNA Binding Proteins Associated with Dengue Virus RNA in Infected Cells Reveals Temporally Distinct Host Factor Requirements Previously, we have described a high-throughput mass spectrometry method termed TUX-MS (thiouracil cross-linking mass spectrometry) to identify host factors that interact with viral RNA during a live infection in cell culture [15] . Development of a quantitative thiouridine cross-linking mass spectrometry (qTUX-MS) method for identification of proteins associated with the DENV RNA TUX-MS can be used to identify host factors by incorporating 4-thiouridine (4sU), a zero-distance cross-linker, into the viral RNA (vRNA) to enable cross-linking of proteins bound to vRNA during a live infection in cell culture [15] . Since some of hnRNPs are known to modulate cellular gene expression in response to dengue infection [60, 61] , we can not rule out that some of the observed effects on virus titers derive from their roles in regulating host mRNAs. Among the numerous qTUX-MS identified factors of interest, our study is the first to demonstrate the involvement of HMCES (or C3orf37) in viral infection. cache = ./cache/cord-002179-v8lpw4r7.txt txt = ./txt/cord-002179-v8lpw4r7.txt === reduce.pl bib === id = cord-272459-w14finxf author = Heaton, Nicholas S. title = Dengue Virus and Autophagy date = 2011-08-04 pages = extension = .txt mime = text/plain words = 3107 sentences = 182 flesch = 40 summary = Recently, it was reported that autophagy plays an indirect role in DENV replication by modulating cellular lipid metabolism. Relevant to DENV infection, a type of selective autophagy termed lipophagy was described, wherein autophagosomes can target cellular stores of lipids known as lipid droplets (LDs) to generate energy for the cell [38] . In subsequent work, the authors reproduced the published results that DENV induces and requires autophagy for robust viral replication [39] . These autophagosomes did not co-localize with markers of the viral replication complex, suggesting that they may play an indirect, non-structural role in DENV replication. Alternatively, DENV infection induces a selective autophagy that is preferentially targeted to lipid droplets, which leads to changes in cellular metabolism. More work, however, is required to show whether the proposed viral triggers of autophagy reproduce all cellular signals and phenotypes that accompany autophagy induction in DENV-infected cells. cache = ./cache/cord-272459-w14finxf.txt txt = ./txt/cord-272459-w14finxf.txt === reduce.pl bib === === reduce.pl bib === id = cord-011390-98xyie7s author = Darcy, Andrew Waleluma title = Multiple arboviral infections during a DENV-2 outbreak in Solomon Islands date = 2020-05-15 pages = extension = .txt mime = text/plain words = 4538 sentences = 236 flesch = 53 summary = An outbreak of dengue-like illness (DLI) that occurred in April 2016 prompted this study, which aimed to determine the population's immunity status and identify the arboviruses circulating in the country. The study objectives were to estimate the seroprevalence of arboviruses in the urban population of Solomon Islands while using qPCR to determine the circulating viruses from the clinical samples; furthermore, to characterize and compare the dengue virus isolated from our study to previous published isolates from the region using the phylogenetic tree analysis. The high positivity rate for anti-DENV IgG antibodies (77.9%) and the high DENV positivity rate detected in the qPCR analysis of the clinical samples (76.3%) were indicative of a high number of secondary infections in the 2016 outbreak. The phylogenetic tree analysis and the serotypespecific qPCR results indicated that there was a single DENV-3 serotype in circulation from 2012 to 2016 in Solomon Islands. cache = ./cache/cord-011390-98xyie7s.txt txt = ./txt/cord-011390-98xyie7s.txt === reduce.pl bib === id = cord-270495-2u072mtp author = Lokida, Dewi title = Diagnosis of COVID-19 in a Dengue-Endemic Area date = 2020-08-05 pages = extension = .txt mime = text/plain words = 1750 sentences = 110 flesch = 53 summary = When SARS-CoV-2 is negative and clinical indication is present (at least fever and thrombocytopenia), DENV NS1 antigen and/or IgM/IgG antibody testing may be performed. Clinicians from Singapore reported two COVID-19 cases that were misdiagnosed as dengue among patients who presented with clinical manifestations and hematology profiles, suggesting dengue infection and false-positive DENV IgM antibody using a rapid diagnostic test (RDT). COVID-19 cases were defined as inpatients who met the COVID-19 criteria based on a predetermined combination of symptoms, laboratory testing, imaging, and risk exposure at Tangerang District Hospital, Indonesia (see Supplemental Table 1 ), and had a positive nasopharyngeal or oropharyngeal real-time RT-PCR for SARS-CoV-2. None of the 42 subjects was positive for dengue NS1 or showed seroconversion or increasing DENV IgM and IgG index values, suggesting no acute DENV infection among these COVID-19 cases. The third patient did not recall having a fever before acute COVID-19 illness, suggesting asymptomatic or mild dengue, the most common presentation of DENV infection. cache = ./cache/cord-270495-2u072mtp.txt txt = ./txt/cord-270495-2u072mtp.txt === reduce.pl bib === id = cord-277547-2vim1wno author = Zandi, Keivan title = Antiviral activity of four types of bioflavonoid against dengue virus type-2 date = 2011-12-28 pages = extension = .txt mime = text/plain words = 4381 sentences = 247 flesch = 52 summary = In the present study, antiviral activity of four types of bioflavonoid against dengue virus type -2 (DENV-2) in Vero cell was evaluated. Daidzein showed a weak anti-dengue activity with IC(50 )= 142.6 μg mL(-1 )when the DENV-2 infected cells were treated after virus adsorption. Although there was no significant direct virucidal activity against DENV-2 by quercetin, continuous treatment of cells from 5 h before virus infection up to 4 days post-infection exhibited anti-dengue activity with IC 50 = 28.9 μg mL -1 (Figure 3a) . There was no significant change in the antiviral activity of daidzein when cells were treated continuously from 5 h before virus infection up to 4 days post infection comparing to its anti-dengue activity for postadsorption treatment (Figure 1 ). To investigate which of the many flavonoids could affect DENV infection, in the present study, we examined the potential effects of quercetin, naringin, hesperetin and daidzein on dengue virus infection of Vero cells. cache = ./cache/cord-277547-2vim1wno.txt txt = ./txt/cord-277547-2vim1wno.txt === reduce.pl bib === id = cord-256732-md1u51va author = Shrivastava, Gaurav title = Inflammasome Fuels Dengue Severity date = 2020-09-10 pages = extension = .txt mime = text/plain words = 8878 sentences = 474 flesch = 41 summary = Inflammasome plays a vital function in the host innate immune system by regulating the secretion of proinflammatory cytokines (IL-1β and IL-18) and subsequent induction of "pyroptosis, " a form of programmed cell death, activated by inflammatory caspases (Fink and Cookson, 2006; Lamkanfi and Dixit, 2014) . IL-1β secretion further enhances the production and release of IL-23 and IL-6 and the association of IL-18, IL-1β, and IL-23 stimulate Th17/γδ T cells to generate pro-inflammatory cytokines (GM-CSF, IL-17A, IL-17F, IL-22,) that create the stage for host adaptive immune responses during DENV infection (Wu et al., 2013a) . Another study demonstrated that DENV infects DC and induces NLRP3 inflammasome activation, triggering ROS production in mitochondria that leads to the discharge of mitochondrial DNA (mtDNA) into the cytosol. (D) DENV activates platelets and induces the secretion of EVs (DV-EXOs and DV-MVs), that results in the CLEC5A and TLR2 mediated release of proinflammatory cytokines and NETs formation in neutrophils contributing to vascular leakage during dengue infection. cache = ./cache/cord-256732-md1u51va.txt txt = ./txt/cord-256732-md1u51va.txt === reduce.pl bib === id = cord-318614-518giv0m author = Tsai, Jih-Jin title = A fully automated sample-to-answer PCR system for easy and sensitive detection of dengue virus in human serum and mosquitos date = 2019-07-10 pages = extension = .txt mime = text/plain words = 4825 sentences = 213 flesch = 49 summary = A pan-dengue virus (DENV) RT-iiPCR, targeting the 5' untranslated region, was validated previously on the semi-automated POCKIT combo system (involving separate devices for nucleic acid extraction and PCR amplification/detection) to offer performance comparable to a laboratory real-time PCR. CONCLUSIONS/SIGNIFICANCE: With performance comparable to a previously validated system, the fully-automated PCR system allows applications of the pan-DENV reagent as a useful tool near points of need to facilitate easy, fast and effective detection of dengue virus and help mitigate versatile public health challenges in the control and management of dengue disease. Testing with the pan-DENV RT-iiPCR, the analytical and clinical performance of the fully automatic POCKIT Central system was comparable to those of the semi-automatic POCKI combo system, which was validated previously to offer performances equivalent to the CDC DENV1-4 real-time RT-PCR for the detection of DENV in human serum [7, 24, 26] . cache = ./cache/cord-318614-518giv0m.txt txt = ./txt/cord-318614-518giv0m.txt === reduce.pl bib === id = cord-286255-ded5t1ai author = Tomashek, Kay M. title = Clinical and epidemiologic characteristics of dengue and other etiologic agents among patients with acute febrile illness, Puerto Rico, 2012–2015 date = 2017-09-13 pages = extension = .txt mime = text/plain words = 6132 sentences = 315 flesch = 53 summary = Blood and oro-nasopharyngeal specimens were tested by RT-PCR and immunodiagnostic methods for infection with dengue viruses (DENV) 1–4, chikungunya virus (CHIKV), influenza A and B viruses (FLU A/B), 12 other respiratory viruses (ORV), enterovirus, Leptospira spp., and Burkholderia pseudomallei. Clinical predictors of laboratory-positive dengue compared to all other AFI etiologies were determined by age and day post-illness onset (DPO) at presentation. By enrolling febrile patients at clinical presentation, we identified unbiased predictors of laboratory-positive dengue as compared to other common causes of AFI. Among 6,349 participants who presented early (<3 DPO) in the clinical course, leukopenia, thrombocytopenia, headache, eye pain, nausea, and dizziness were significant positive predictors of laboratory-positive dengue as compared to all other AFI cases across all age groups ( Table 5 ). Of note, 6% of participants !65 years old had dengue as a cause of AFI, a finding comparable to a Puerto Rico study in which 5% of 17,666 laboratory-positive dengue cases detected by surveillance were !65 years old [36] . cache = ./cache/cord-286255-ded5t1ai.txt txt = ./txt/cord-286255-ded5t1ai.txt === reduce.pl bib === === reduce.pl bib === === reduce.pl bib === id = cord-318120-vfznyyz6 author = Dauner, Allison L. title = Development of a pan-serotype reverse transcription loop-mediated isothermal amplification assay for the detection of dengue virus date = 2015-05-15 pages = extension = .txt mime = text/plain words = 5265 sentences = 288 flesch = 48 summary = title: Development of a pan-serotype reverse transcription loop-mediated isothermal amplification assay for the detection of dengue virus To facilitate needed diagnosis, we developed and optimized a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay that detects all 4 serotypes of dengue virus (DENV). Development of simple and rapid assay to detect viral RNA of tick-borne encephalitis virus by reverse transcription-loop-mediated isothermal amplification A real-time reverse transcription loop-mediated isothermal amplification assay for the rapid detection of yellow fever virus Rapid detection and differentiation of dengue virus serotypes by NS1 specific reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay in patients presenting to a tertiary care hospital in Rapid detection and differentiation of dengue virus serotypes by a real-time reverse transcription-loopmediated isothermal amplification assay Development and evaluation of reverse transcription-loop-mediated isothermal amplification assay for rapid and real-time detection of Japanese encephalitis virus cache = ./cache/cord-318120-vfznyyz6.txt txt = ./txt/cord-318120-vfznyyz6.txt === reduce.pl bib === === reduce.pl bib === id = cord-297662-slmlhqnb author = Yap, Sally S. L. title = Dengue Virus Glycosylation: What Do We Know? date = 2017-07-25 pages = extension = .txt mime = text/plain words = 11116 sentences = 526 flesch = 48 summary = In this review, we seek to provide a comprehensive summary of the current knowledge on protein glycosylation in DENV, and its role in virus biogenesis, host cell receptor interaction and disease pathogenesis. Since high mannose binding DC-SIGN interacts only with N67 glycans on the viral surface (Pokidysheva et al., 2006) and N153-glycan is dispensable for virus production in mosquito and mammalian cells (Bryant et al., 2007) , this suggests that N153 glycans may serve a distinct function from N67 glycans in DEN pathogenesis possibly via interaction with an unknown fucose binder or act as a viral glycan shield. Finally, N153 deglycosylated (N153 − ) DENV mutant displayed reduced infectivity (10-fold lower) in both mammalian and mosquito cells compared to WT, possibly due to impaired virus entry process (Lee et al., 1997; Hacker et al., 2009) , whereby loss of the N153-glycan affected the conformational stability of E proteins and led to premature exposure of the fusion peptide (Yoshii et al., 2013) . N-linked glycosylation of dengue virus NS1 protein modulates secretion, cell-surface expression, hexamer stability, and interactions with human complement cache = ./cache/cord-297662-slmlhqnb.txt txt = ./txt/cord-297662-slmlhqnb.txt === reduce.pl bib === id = cord-011968-abd891ej author = Lai, Yen-Chung title = Roles of Macrophage Migration Inhibitory Factor in Dengue Pathogenesis: From Pathogenic Factor to Therapeutic Target date = 2020-06-12 pages = extension = .txt mime = text/plain words = 5705 sentences = 268 flesch = 38 summary = Macrophage migration inhibitory factor (MIF) is a pleiotropic proinflammatory cytokine that mediates diverse immune responses, and the serum level of MIF positively correlates with disease severity in patients with dengue. In another study, it was demonstrated that live DENV2-induced endoplasmic reticulum (ER) stress is required for autophagy activation, viral replication and pathogenesis in HuH-7 and A549 cells [57] . In our study, since UV-inactivated viral particles could not induce MIF secretion or expression, it is possible that DENV infection triggered RNA sensing or pattern recognition receptor (PRR) activation, which was followed by the release of preformed MIF from the cytosol through the vesicle trafficking secretory pathway. Macrophage migration inhibitory factor induced by dengue virus infection increases vascular permeability Minocycline suppresses dengue virus replication by down-regulation of macrophage migration inhibitory factor-induced autophagy Dengue virus nonstructural protein 1 induces vascular leakage through macrophage migration inhibitory factor and autophagy cache = ./cache/cord-011968-abd891ej.txt txt = ./txt/cord-011968-abd891ej.txt === reduce.pl bib === === reduce.pl bib === id = cord-282742-eyukbot7 author = Diosa-Toro, Mayra title = Arthropod-Borne Flaviviruses and RNA Interference: Seeking New Approaches for Antiviral Therapy date = 2013-02-20 pages = extension = .txt mime = text/plain words = 6493 sentences = 364 flesch = 48 summary = Geiss, Pierson, and Diamond (2005) observed that siRNAs targeting the C gene had no effect on virus replication when transfected into cells 10 h after WNV infection using lipid-based reagents. In addition, no significant reduction in viral protein or RNA levels was seen in WNV replicon-expressing cells transfected with siRNAs targeting the NS3 gene using lipid-based reagents. Also, a recent report showed that siRNA toward the TNF-a gene reduced cytokine response in DENV-infected DCs, highlighting the potential of targeted RNAi-based approaches to simultaneously decrease viral replication and the detrimental host immune response (Subramanya et al., 2010) . In addition, it has been shown that WNV (Chotkowski et al., 2008) and DENV (Mukherjee & Hanley, 2010) infection (Mukherjee & Hanley, 2010) of Drosophila cell lines induce functional virus-specific siRNAs that promote a protective RNAi response. So far we have described the antiviral effect of the RNAi mechanism induced by exogenous delivery of siRNA or precursors, and how cellular miRNA can target sequences artificially introduced within the genome of flaviviruses. cache = ./cache/cord-282742-eyukbot7.txt txt = ./txt/cord-282742-eyukbot7.txt === reduce.pl bib === id = cord-276718-3lujp0oy author = Neeraja, M. title = Rapid detection and differentiation of dengue virus serotypes by NS1 specific reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay in patients presenting to a tertiary care hospital in Hyderabad, India date = 2014-10-24 pages = extension = .txt mime = text/plain words = 6142 sentences = 296 flesch = 54 summary = title: Rapid detection and differentiation of dengue virus serotypes by NS1 specific reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay in patients presenting to a tertiary care hospital in Hyderabad, India In the present study, the standardization and validation of a one step, four tube reverse transcription loop-mediated isothermal amplification assay (RT-LAMP) for rapid detection and serotyping of the DENV targeting NS1 gene using the Genie® II flourometer was carried out. In the present study, the RT-LAMP assay was developed for the detection and serotyping of DENV infection targeting the serotype specific regions of the NS1 gene using a real-time flourometer (Genie ® II from Optigene, U.K.). The performance of the RT-LAMP assay was validated by testing the samples simultaneously by the CDC real time PCR that is most sensitive and specific method for detection and differentiation of the DENV (CDC Dengue). Rapid detection and differentiation of dengue virus serotypes by a real-time reverse transcription-loop-mediated isothermal amplification assay cache = ./cache/cord-276718-3lujp0oy.txt txt = ./txt/cord-276718-3lujp0oy.txt === reduce.pl bib === id = cord-285538-3ah05ijf author = Halstead, Scott B. title = Chapter 19 Pathogenic Exploitation of Fc Activity date = 2014-12-31 pages = extension = .txt mime = text/plain words = 7637 sentences = 426 flesch = 39 summary = 3, 4 While follow-up research in the 1960s suggested that this phenomenon resulted from the stabilization of MVEV by antibodies, 5 a different explanation emerged when sequential infections in humans with dengue viruses (DENVs) were shown to produce severe disease (dengue hemorrhagic fever, DHF). Unexpectedly, the incubation of Ross River virus (RRV) with diluted RRV antiserum resulted in enhanced infection in mouse macrophage cell lines and in primary human monocytes/macrophages via innate immune suppression involving reduced production of reactive nitrogen radicals via NOS2 and a downregulation of TNF-α and IFN-β production through abolished interferon regulatory factor 1 (IRF-1) and nuclear factor-κB gene expression. 43 Note has been made above of the possible role of different Fcγ receptors in mediating ADE in macrophages in leishmania mouse models and the interesting observation suggesting that ligation of FcγRIIB may affect the fate of large immune complexes during the immune clearance phase of dengue infections. cache = ./cache/cord-285538-3ah05ijf.txt txt = ./txt/cord-285538-3ah05ijf.txt === reduce.pl bib === id = cord-011026-iapgkz0p author = El-Bitar, Alaa M. H. title = Smp76, a Scorpine-Like Peptide Isolated from the Venom of the Scorpion Scorpio maurus palmatus, with a Potent Antiviral Activity Against Hepatitis C Virus and Dengue Virus date = 2019-07-06 pages = extension = .txt mime = text/plain words = 5247 sentences = 296 flesch = 53 summary = title: Smp76, a Scorpine-Like Peptide Isolated from the Venom of the Scorpion Scorpio maurus palmatus, with a Potent Antiviral Activity Against Hepatitis C Virus and Dengue Virus Smp76 antiviral activity was evaluated using a cell culture technique utilizing Huh7it-1, Vero/SLAM, HCV (JFH1, genotype 2a) and DENV (Trinidad 1751, type 2). For dengue virus infectivity, serially diluted venom fractions and Smp76 were mixed with fixed amount of DENV and incubated for 2 h at 37 °C. The above results suggest that the Smp76 directly affects HCV particles and/or host cells in the culture medium to inhibit the viral infection and does not have an antiviral effect in the cells. To determine whether the antiviral activity of Smp76 peptide (previously described) was specific to HCV and DENV, a Schematic of infection assay. The exact mechanism by which Smp76 exerts its antiviral activity against HCV and DENV to inhibit infecting their target cells need further studies. cache = ./cache/cord-011026-iapgkz0p.txt txt = ./txt/cord-011026-iapgkz0p.txt === reduce.pl bib === id = cord-317169-qlqavi4t author = Chiow, K.H. title = Evaluation of antiviral activities of Houttuynia cordata Thunb. extract, quercetin, quercetrin and cinanserin on murine coronavirus and dengue virus infection date = 2015-12-19 pages = extension = .txt mime = text/plain words = 4302 sentences = 225 flesch = 50 summary = (Saururaceae) and three of its constituent flavonoids (quercetin, quercitrin and rutin) against murine coronavirus and dengue virus (DENV). cordata and flavonoids were assessed using virus neutralization tests against mouse hepatitis virus (MHV) and DENV type 2 (DENV-2). Certain flavonoids exhibited comparatively weaker antiviral activity, notably quercetin which could inhibit both MHV and DENV-2. This was followed by quercitrin which could inhibit DENV-2 but not MHV, whereas rutin did not exert any inhibitory effect on either virus. cordata contribute to the superior antiviral efficacy of the EA fraction which lacked cytotoxicity in vitro and acute toxicity in vivo. cordata exhibited anti-MHV activity at a MIC of 0.98 mg/mL without any apparent cytotoxic effects on CCL9.1 cells. cordata and its flavonoid component, quercetin, could inhibit both MHV and DENV-2 in vitro. Houttuynia cordata extracts and constituents inhibit the infectivity of dengue virus type 2 in vitro cache = ./cache/cord-317169-qlqavi4t.txt txt = ./txt/cord-317169-qlqavi4t.txt === reduce.pl bib === === reduce.pl bib === === reduce.pl bib === id = cord-355489-tkvfneje author = Mendez, Jairo A title = Phylogenetic history demonstrates two different lineages of dengue type 1 virus in Colombia date = 2010-09-14 pages = extension = .txt mime = text/plain words = 4615 sentences = 235 flesch = 48 summary = Yet, the phylogenetic relationships between strains isolated along the covered period of time suggests that viral strains detected in some years, although belonging to the same genotype V, have different recent origins corresponding to multiple re-introduction events of viral strains that were circulating in neighbor countries. Due to the importance of DENV in public health, the particular goals of this research were to reconstruct the phylogenetic history of DENV-1 and to date the phylogenetic tree using isolation time as calibration points to establish date of introduction of virus and rate evolution patterns of virus in Colombia. Previously reported genotypes were represented in the tree and placed most of the Colombian isolates nesting in the genotype V clade (America, Africa) and were closely related to Argentina, Brazil and Paraguay virus strains. cache = ./cache/cord-355489-tkvfneje.txt txt = ./txt/cord-355489-tkvfneje.txt === reduce.pl bib === === reduce.pl bib === === reduce.pl bib === id = cord-328661-spxgox52 author = Yu, Jianhai title = Epidemiological and Evolutionary Analysis of Dengue-1 Virus Detected in Guangdong during 2014: Recycling of Old and Formation of New Lineages date = 2019-08-05 pages = extension = .txt mime = text/plain words = 5834 sentences = 314 flesch = 50 summary = The lack of sufficient epidemiological data and evidence on the local mosquito-borne DENV emphasizes the importance of studying the molecular evolutionary features and establishing a well-established phylogenetic tree for dengue prevention and control in Guangdong. Since 1990, however, DENV1 has been mainly isolated from the infected cases, and its continued existence in Guangdong Province indicated that endemic infectious agents of dengue may be circulating locally. With the epidemiological data since 2005 supplied by the Guangdong Provincial CDC, we studied phylogenetics, molecular characteristics, and epidemiology to strengthen the foundational research of DENV1 for the prevention of large-scale dengue epidemics, providing preventive and control measures of DF with important evidence. Based on representative strains of the E gene in lineages of the 2014 outbreak, as well as the molecular evolution database, we analyzed molecular characterization and possibility of local circulation for DENV1 since 2005 in Guangdong. cache = ./cache/cord-328661-spxgox52.txt txt = ./txt/cord-328661-spxgox52.txt === reduce.pl bib === id = cord-330743-o11d0aa1 author = Yu, Xi title = Broad-spectrum virucidal activity of bacterial secreted lipases against flaviviruses, SARS-CoV-2 and other enveloped viruses date = 2020-05-25 pages = extension = .txt mime = text/plain words = 4470 sentences = 245 flesch = 54 summary = Herein, we identified 2 secreted bacterial lipases from a Chromobacterium bacterium, named Chromobacterium antiviral effector-1 (CbAE-1) and CbAE-2, with a broad-spectrum virucidal activity against dengue virus (DENV), Zika virus (ZIKV), severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), human immunodeficiency virus (HIV) and herpes simplex virus (HSV). Incubation of the culture supernatant but not the bacterial lysates resulted in significant suppression of DENV ( Figure 1B ) and ZIKV ( Figure 1C ) infectivity in Vero cells, indicating that an extracellular effector(s) secreted by Csp_BJ was responsible for viral inhibition. DENV, ZIKV, HSV-1 and SARS-CoV-2 virus stocks were diluted to 50 plaque-forming units (pfu) per ml and incubated untreated or with a serial dilution of the CbAEs in five-fold steps at 37°C for 1 hr before being added onto Vero cell monolayers for 2 hr of infection. cache = ./cache/cord-330743-o11d0aa1.txt txt = ./txt/cord-330743-o11d0aa1.txt === reduce.pl bib === id = cord-306204-rkher4ly author = Pong, Lian Yih title = Anti-dengue virus serotype 2 activity of tannins from porcupine dates date = 2020-05-20 pages = extension = .txt mime = text/plain words = 6060 sentences = 318 flesch = 54 summary = Vero cells grown in 24-well plates were infected with 30-50 FFU of DENV-2 in the presence or absence of BDTF, PDTF, methanol crude extracts, tannic acids of FT and ST at maximum non-toxic concentration (MNTC) of 100 µg/ mL, 50 µg/mL, 200 µg/mL, 50 µg/mL and 25 µg/mL respectively for 1 h at 4 °C. To study whether tannins in the porcupine dates are bioactive against dengue infection, we have isolated the tannin fractions from MBD and MPD for Besides that, both BDTF and PDTF demonstrated similar and higher virucidal activity against DENV-2 when compared to their methanol crude extracts. " indicates not significant Fig. 9 Effect of pre-treatment with porcupine dates extracts and tannic acids on DENV-2 infection in Vero cells. The antiviral activity ascribed to the interaction between the extracts and host cells was also investigated; pre-treating the cells with BDTF prevented the DENV-2 infection by at least 75% and similar result was observed for FT tannic acid. cache = ./cache/cord-306204-rkher4ly.txt txt = ./txt/cord-306204-rkher4ly.txt === reduce.pl bib === id = cord-345898-a6vt8kso author = Ren, Linzhu title = Live Cell Reporter Systems for Positive-Sense Single Strand RNA Viruses date = 2016-01-04 pages = extension = .txt mime = text/plain words = 6000 sentences = 289 flesch = 38 summary = There are three types of cell-based reporter systems that express certain reporter protein for positive-sense single strand RNA virus infections. An RVP is a type of virus-like particle (VLP) composed of viral structural proteins and a self-replicating replicon RNA containing a reporter gene [17, 63] . However, when the cells were infected with the specific virus, the viral RdRp was provided by the virus and the defective replicon was activated, resulting in high-level expression of the reporter gene, which could be easily examined [44] . However, when the cells were infected with the specific virus, the viral RdRp was provided by the virus and the defective replicon was activated, resulting in high-level expression of the reporter gene, which could be easily examined [44] . Development of Dengue type-2 virus replicons expressing GFP reporter gene in study of viral RNA replication cache = ./cache/cord-345898-a6vt8kso.txt txt = ./txt/cord-345898-a6vt8kso.txt === reduce.pl bib === id = cord-307219-okvvajms author = Lazzarini, Luca title = First autochthonous dengue outbreak in Italy, August 2020 date = 2020-09-10 pages = extension = .txt mime = text/plain words = 1887 sentences = 94 flesch = 54 summary = In August 2020, during the coronavirus disease (COVID-19) pandemic, five locally acquired cases of dengue virus type 1 were detected in a family cluster in Vicenza Province, North-East Italy where Aedes albopictus mosquitoes are endemic. In August 2020, during the coronavirus disease (COVID-19) pandemic, five locally acquired cases of dengue virus type 1 were detected in a family cluster in Vicenza Province, North-East Italy where Aedes albopictus mosquitoes are endemic. She was investigated for West Nile virus, Usutu virus, dengue virus (DENV), chikungunya virus (CHIKV) and Zika virus (ZIKV) by molecular and serology testing, because she reported that a family member (Case 1) had had similar symptoms after a recent travel in Indonesia. The surveillance, active between June and November, aims to increase the detection rate of DENV and CHIKV (since 2015, also ZIKV) infection in travellers from endemic areas and to promptly identify potential autochthonous cases. cache = ./cache/cord-307219-okvvajms.txt txt = ./txt/cord-307219-okvvajms.txt === reduce.pl bib === id = cord-353241-ityhcak7 author = Zhu, Hanliang title = IoT PCR for pandemic disease detection and its spread monitoring date = 2020-01-15 pages = extension = .txt mime = text/plain words = 4300 sentences = 208 flesch = 54 summary = Considerable effort has been invested in the development of portable, user-friendly, and cost-effective systems for point-of-care (POC) diagnostics, which could also create an Internet of Things (IoT) for healthcare via a global network. Connecting the easy to use and cost-effective POC devices providing the DENV diagnoses via a mobile network would create an Internet of Things (IoT) [15] for healthcare [16, 17] , an essential tool to tackle any infectious disease outbreak. Prior to testing on an IoT PCR device, we verified the master mix performance and its values of critical threshold (C T ) and the melting temperature (T M ) using a commercial real-time PCR system (Supplementary Section A) beginning with a hot start at 95°C for 30 s followed by 40 cycles of PCR amplification consisting of DNA denaturation at 95°C for 8 s, primer annealing at 60°C for 30 s, and DNA sequence elongation at 72°C for 10 s, then followed by melting curve analysis (MCA) from 72°C to 95°C. cache = ./cache/cord-353241-ityhcak7.txt txt = ./txt/cord-353241-ityhcak7.txt === reduce.pl bib === === reduce.pl bib === id = cord-342157-qjyooq68 author = King, Chwan-Chuen title = Comparative analysis of full genomic sequences among different genotypes of dengue virus type 3 date = 2008-05-21 pages = extension = .txt mime = text/plain words = 5860 sentences = 277 flesch = 48 summary = In our study, complete genomic sequencing of DENV-3 strains collected from different geographical locations and isolation years were determined and the sequence diversity as well as selection pressure sites in the DENV genome other than within the E gene were also analyzed. RESULTS: Using maximum likelihood and Bayesian approaches, our phylogenetic analysis revealed that the Taiwan's indigenous DENV-3 isolated from 1994 and 1998 dengue/DHF epidemics and one 1999 sporadic case were of the three different genotypes – I, II, and III, each associated with DENV-3 circulating in Indonesia, Thailand and Sri Lanka, respectively. Compared to the prototype strain H87, several unique amino acid substitutions that serve as unique signature sites for each genotype were found within the full genomic sequences of the selected DENV-3 isolates from Taiwan or other countries and are listed by the order of the gene in Table 3 . cache = ./cache/cord-342157-qjyooq68.txt txt = ./txt/cord-342157-qjyooq68.txt === reduce.pl bib === id = cord-344020-8poerd09 author = Vermeulen, Tom D title = Autochthonous dengue in two Dutch tourists visiting Département Var, southern France, July 2020 date = 2020-10-01 pages = extension = .txt mime = text/plain words = 1827 sentences = 110 flesch = 54 summary = We report dengue virus (DENV) infection in two Dutch tourists who visited Département Var, southern France, in July and August 2020. We report dengue virus (DENV) infection in two Dutch tourists who visited Département Var, southern France, in July and August 2020. As some autochthonous dengue cases have occurred in Europe in recent years, awareness among physicians and public health experts about possible intermittent presence of DENV in southern Europe is important to minimise delay in diagnosis and treatment. As some autochthonous dengue cases have occurred in Europe in recent years, awareness among physicians and public health experts about possible intermittent presence of DENV in southern Europe is important to minimise delay in diagnosis and treatment. On August 27, upon confirmation of the serological results, Patient 1 was reported by the RIVM to the French authorities through the Early Warning and Response System of the European Union as an autochthonous DENV infection probably acquired in France with cross-border implication. cache = ./cache/cord-344020-8poerd09.txt txt = ./txt/cord-344020-8poerd09.txt === reduce.pl bib === === reduce.pl bib === === reduce.pl bib === === reduce.pl bib === id = cord-282204-j1slaefb author = Silva, José V.J. title = A scoping review of Chikungunya virus infection: epidemiology, clinical characteristics, viral co-circulation complications, and control date = 2018-12-31 pages = extension = .txt mime = text/plain words = 8010 sentences = 464 flesch = 43 summary = de; Oliveira, Renato A.S.; Durães-Carvalho, Ricardo; Lopes, Thaísa R.R.; Silva, Daisy E.A.; Gil, Laura H.V.G. title: A scoping review of Chikungunya virus infection: epidemiology, clinical characteristics, viral co-circulation complications, and control Laboratory tests for specific diagnosis of CHIKV infection are based on virus isolation, viral RNA detection and serology (Johnson et al., 2016) . Anti-CHIKV candidates that have been already tested in humans and/or animals include inactivated-, attenuated-, virus like particle-(VLP), DNA-and chimeric vaccines (Eckels et al., 1970; Levitt et al., 1986; Muthumani et al., 2008; Wang et al., 2008; Tiwari et al., 2009; Sharma et al., 2012 Akahata et al., 2010 Plante et al., 2011; Wang et al., 2011; Gorchakov et al., 2012; Brandler et al., 2013; Chang et al., 2014; García-Arriaza et al., 2014; Tretyakova et al., 2014; van den Doel et al., 2014; Erasmus et al., 2017) . cache = ./cache/cord-282204-j1slaefb.txt txt = ./txt/cord-282204-j1slaefb.txt === reduce.pl bib === === reduce.pl bib === id = cord-355179-wmfwl2bh author = Jung, Eunhye title = Neutralization of Acidic Intracellular Vesicles by Niclosamide Inhibits Multiple Steps of the Dengue Virus Life Cycle In Vitro date = 2019-06-18 pages = extension = .txt mime = text/plain words = 5305 sentences = 271 flesch = 44 summary = Overall, the data indicate that the antiviral activity of niclosamide during the early stage of the DENV life cycle correlates with the neutralization profile of the low-pH compartments, suggesting that blocking endosomal acidification results in the inhibition of viral genome replication and polyprotein processing, which further impedes viral protein expression and virus production. In this study, we found that neutralization of low-pH intracellular compartments by niclosamide not only inhibited the early stage of the DENV viral life cycle, such as viral RNA replication, independent of the entry step but also the late stage, specifically, the maturation of virus particles into infectious virions. Indeed, niclosamide treatment www.nature.com/scientificreports www.nature.com/scientificreports/ during the first 6 h of infection reduced DENV replication in BHK-21 cells harbouring dengue replicons to a level comparable to that of ribavirin, suggesting that the drug affects viral RNA replication and/or translation independent of its effect on entry, membrane fusion and genome release. cache = ./cache/cord-355179-wmfwl2bh.txt txt = ./txt/cord-355179-wmfwl2bh.txt ===== Reducing email addresses cord-270495-2u072mtp cord-328661-spxgox52 Creating transaction Updating adr table ===== Reducing keywords cord-002413-795wuqz5 cord-002085-e7xwb03g cord-276364-zyw5aukk cord-002426-5e1xn7kj cord-300648-ixiam7qr cord-253480-qchrw337 cord-011012-5mev3otu cord-278260-3o91v72a cord-033331-giku34r9 cord-002179-v8lpw4r7 cord-272459-w14finxf cord-273388-615acz0l cord-011390-98xyie7s cord-270495-2u072mtp cord-277547-2vim1wno cord-286255-ded5t1ai cord-256732-md1u51va cord-318614-518giv0m cord-274129-vaygaqe5 cord-002581-r7mskri0 cord-318120-vfznyyz6 cord-297662-slmlhqnb cord-011968-abd891ej cord-326840-piv6h7oq cord-282742-eyukbot7 cord-025181-eg108wcd cord-276718-3lujp0oy cord-011026-iapgkz0p cord-285538-3ah05ijf cord-317169-qlqavi4t cord-320091-2lrqubdl cord-355489-tkvfneje cord-295191-xu26mvc3 cord-290385-0smnl70i cord-294842-aesiff1f cord-328661-spxgox52 cord-330743-o11d0aa1 cord-306204-rkher4ly cord-345898-a6vt8kso cord-353241-ityhcak7 cord-307219-okvvajms cord-355610-7xy4s483 cord-342157-qjyooq68 cord-355906-yeaw9nr8 cord-303818-z3js3mr4 cord-344020-8poerd09 cord-319174-pbqjg7hf cord-282204-j1slaefb cord-273065-peqz7okh cord-355179-wmfwl2bh Creating transaction Updating wrd table ===== Reducing urls cord-002085-e7xwb03g cord-002413-795wuqz5 cord-011012-5mev3otu cord-274129-vaygaqe5 cord-253480-qchrw337 cord-033331-giku34r9 cord-318120-vfznyyz6 cord-326840-piv6h7oq cord-276718-3lujp0oy cord-328661-spxgox52 cord-353241-ityhcak7 cord-355610-7xy4s483 cord-355179-wmfwl2bh cord-303818-z3js3mr4 cord-273065-peqz7okh Creating transaction Updating url table ===== Reducing named entities cord-002413-795wuqz5 cord-002085-e7xwb03g cord-002426-5e1xn7kj cord-011012-5mev3otu cord-276364-zyw5aukk cord-300648-ixiam7qr cord-253480-qchrw337 cord-033331-giku34r9 cord-278260-3o91v72a cord-002179-v8lpw4r7 cord-272459-w14finxf cord-273388-615acz0l cord-011390-98xyie7s cord-270495-2u072mtp cord-256732-md1u51va cord-277547-2vim1wno cord-318614-518giv0m cord-286255-ded5t1ai cord-274129-vaygaqe5 cord-002581-r7mskri0 cord-318120-vfznyyz6 cord-326840-piv6h7oq cord-011968-abd891ej cord-297662-slmlhqnb cord-025181-eg108wcd cord-282742-eyukbot7 cord-276718-3lujp0oy cord-285538-3ah05ijf cord-011026-iapgkz0p cord-320091-2lrqubdl cord-317169-qlqavi4t cord-295191-xu26mvc3 cord-355489-tkvfneje cord-290385-0smnl70i cord-294842-aesiff1f cord-328661-spxgox52 cord-330743-o11d0aa1 cord-306204-rkher4ly cord-307219-okvvajms cord-345898-a6vt8kso cord-355610-7xy4s483 cord-353241-ityhcak7 cord-355906-yeaw9nr8 cord-303818-z3js3mr4 cord-342157-qjyooq68 cord-319174-pbqjg7hf cord-282204-j1slaefb cord-273065-peqz7okh cord-344020-8poerd09 cord-355179-wmfwl2bh Creating transaction Updating ent table ===== Reducing parts of speech cord-002085-e7xwb03g cord-002413-795wuqz5 cord-276364-zyw5aukk cord-278260-3o91v72a cord-253480-qchrw337 cord-272459-w14finxf cord-002426-5e1xn7kj cord-270495-2u072mtp cord-011390-98xyie7s cord-011012-5mev3otu cord-277547-2vim1wno cord-300648-ixiam7qr cord-273388-615acz0l cord-318614-518giv0m cord-002179-v8lpw4r7 cord-033331-giku34r9 cord-274129-vaygaqe5 cord-256732-md1u51va cord-286255-ded5t1ai cord-002581-r7mskri0 cord-326840-piv6h7oq cord-318120-vfznyyz6 cord-011968-abd891ej cord-025181-eg108wcd cord-282742-eyukbot7 cord-276718-3lujp0oy cord-285538-3ah05ijf cord-011026-iapgkz0p cord-317169-qlqavi4t cord-297662-slmlhqnb cord-320091-2lrqubdl cord-295191-xu26mvc3 cord-355489-tkvfneje cord-328661-spxgox52 cord-330743-o11d0aa1 cord-307219-okvvajms cord-353241-ityhcak7 cord-344020-8poerd09 cord-290385-0smnl70i cord-345898-a6vt8kso cord-306204-rkher4ly cord-303818-z3js3mr4 cord-342157-qjyooq68 cord-355610-7xy4s483 cord-319174-pbqjg7hf cord-294842-aesiff1f cord-355906-yeaw9nr8 cord-273065-peqz7okh cord-355179-wmfwl2bh cord-282204-j1slaefb Creating transaction Updating pos table Building ./etc/reader.txt cord-294842-aesiff1f cord-290385-0smnl70i cord-282204-j1slaefb cord-256732-md1u51va cord-355906-yeaw9nr8 cord-297662-slmlhqnb number of items: 50 sum of words: 176,096 average size in words: 5,336 average readability score: 47 nouns: virus; dengue; infection; cells; protein; cell; replication; study; viruses; fever; disease; cases; proteins; assay; control; time; infections; activity; analysis; antibody; host; studies; patients; vaccine; antibodies; response; data; membrane; blood; mice; autophagy; detection; gene; transmission; type; role; results; system; samples; mosquito; expression; activation; treatment; diseases; serotypes; serotype; production; sequence; effect; ° verbs: using; shows; induced; infected; based; reported; including; identified; detect; suggesting; follows; found; associates; binding; performing; increases; observed; containing; mediated; develop; indicated; inhibit; required; provide; demonstrate; neutralizing; determine; reduce; compared; described; results; causes; targeting; enhancing; treated; tested; led; occur; incubated; borne; involving; collecting; expressed; derived; revealed; added; isolate; obtain; evaluated; generated adjectives: viral; human; clinical; antiviral; specific; severe; different; immune; high; non; anti; positive; infectious; cellular; acute; dependent; molecular; infected; first; single; several; important; real; structural; recent; similar; significant; new; global; major; endemic; primary; effective; low; hemorrhagic; like; rapid; potential; early; complex; many; large; negative; multiple; possible; small; higher; available; various; flavivirus adverbs: also; however; well; respectively; previously; highly; significantly; therefore; furthermore; ns3; recently; currently; together; moreover; subsequently; often; encephalitis; directly; still; even; prior; especially; fully; interestingly; first; briefly; mainly; finally; usually; approximately; early; particularly; less; importantly; similarly; additionally; primarily; least; specifically; likely; indeed; commonly; rather; probably; clinically; widely; notably; efficiently; relatively; rapidly pronouns: we; it; its; their; our; they; i; them; he; her; she; his; us; itself; your; ifitm3; you; one; themselves; cbae-1; him; smp76; il-1β; ‫ﺍ‬; tv005; serotype-; pla2g16; nsp1; ns3/4a; epa)registered; cxcr7 proper nouns: DENV; RNA; PCR; Fig; ZIKV; DENV-2; RT; Zika; C; CHIKV; Aedes; MIF; WNV; China; Dengue; IFN; West; HCV; Nile; Vero; CBX; SARS; LAMP; IRAV; ER; DHF; II; IFITM3; Table; ADE; IgG; MS; Guangdong; T; IgM; Chikungunya; Fubc; m366.6; Health; ELISA; DF; Brazil; USA; Virus; Asia; siRNA; PBS; M; Ae; IL-10 keywords: denv; virus; rna; dengue; pcr; denv-2; aedes; zikv; infection; hcv; wnv; sars; zika; replication; protein; lamp; ifn; chikv; cell; brazil; autophagy; ade; yucatan; west; vero; vaccine; uprt; tirs; taiwan; system; study; solomon; smp76; rico; puerto; pockit; pll; pfu; pdtf; p1f12; ns1; nlrp3; nile; niclosamide; mov10; moi; mif; mhv; membrane; m366.6 one topic; one dimension: virus file(s): https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5309953/ titles(s): IRAV (FLJ11286), an Interferon-Stimulated Gene with Antiviral Activity against Dengue Virus, Interacts with MOV10 three topics; one dimension: virus; dengue; dengue file(s): https://doi.org/10.3390/v6072826, https://www.sciencedirect.com/science/article/pii/S016344531600061X, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7542575/ titles(s): Membranous Replication Factories Induced by Plus-Strand RNA Viruses | Zika fever and congenital Zika syndrome: An unexpected emerging arboviral disease | The TIRS trial: protocol for a cluster randomized controlled trial assessing the efficacy of preventive targeted indoor residual spraying to reduce Aedes-borne viral illnesses in Merida, Mexico five topics; three dimensions: virus denv dengue; dengue denv virus; virus cells denv; protein dengue denv; antiviral activity cells file(s): https://www.ncbi.nlm.nih.gov/pubmed/25455901/, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7542575/, https://www.ncbi.nlm.nih.gov/pubmed/33014899/, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7223326/, https://doi.org/10.1101/2020.05.22.109900 titles(s): Rapid detection and differentiation of dengue virus serotypes by NS1 specific reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay in patients presenting to a tertiary care hospital in Hyderabad, India | The TIRS trial: protocol for a cluster randomized controlled trial assessing the efficacy of preventive targeted indoor residual spraying to reduce Aedes-borne viral illnesses in Merida, Mexico | Inflammasome Fuels Dengue Severity | Production and immunogenicity of Fubc subunit protein redesigned from DENV envelope protein | Broad-spectrum virucidal activity of bacterial secreted lipases against flaviviruses, SARS-CoV-2 and other enveloped viruses Type: cord title: keyword-denv-cord date: 2021-05-24 time: 23:22 username: emorgan patron: Eric Morgan email: emorgan@nd.edu input: keywords:denv ==== make-pages.sh htm files ==== make-pages.sh complex files ==== make-pages.sh named enities ==== making bibliographics id: cord-295191-xu26mvc3 author: Avirutnan, Panisadee title: Complement and its role in protection and pathogenesis of flavivirus infections date: 2008-12-30 words: nan sentences: nan pages: flesch: nan cache: txt: summary: abstract: The complement system is a family of serum and cell surface proteins that recognize pathogen-associated molecular patterns, altered-self ligands, and immune complexes. Activation of the complement cascade triggers several antiviral functions including pathogen opsonization and/or lysis, and priming of adaptive immune responses. In this review, we will examine the role of complement activation in protection and/or pathogenesis against infection by Flaviviruses, with an emphasis on experiments with West Nile and Dengue viruses. url: https://www.sciencedirect.com/science/article/pii/S0264410X08016083 doi: 10.1016/j.vaccine.2008.11.061 id: cord-320091-2lrqubdl author: Badawi, Alaa title: Prevalence of chronic comorbidities in dengue fever and West Nile virus: A systematic review and meta-analysis date: 2018-07-10 words: nan sentences: nan pages: flesch: nan cache: txt: summary: abstract: BACKGROUND: Flavivirus diseases such as dengue fever (DENV), West Nile virus (WNV), Zika and yellow fever represent a substantial global public health concern. Preexisting chronic conditions such as cardiovascular diseases, diabetes, obesity, and asthma were thought to predict risk of progression to severe infections. OBJECTIVE: We aimed to quantify the frequency of chronic comorbidities in flavivirus diseases to provide an estimate for their prevalence in severe and non-severe infections and examine whether chronic diseases contribute to the increased risk of severe viral expression. METHODS: We conducted a comprehensive search in PubMed, Ovid MEDLINE(R), Embase and Embase Classic and grey literature databases to identify studies reporting prevalence estimates of comorbidities in flavivirus diseases. Study quality was assessed with the risk of bias tool. Age-adjusted odds ratios (ORs) were estimated for severe infection in the presence of chronic comorbidities. RESULTS: We identified 65 studies as eligible for inclusion for DENV (47 studies) and WNV (18 studies). Obesity and overweight (i.e., BMI> 25 kg/m(2), prevalence: 24.5%, 95% CI: 18.6–31.6%), hypertension (17.1%, 13.3–21.8%) and diabetes (13.3%, 9.3–18.8%) were the most prevalent comorbidities in DENV. However, hypertension (45.0%, 39.1–51.0%), diabetes (24.7%, 20.2–29.8%) and heart diseases (25.6%, 19.5–32.7%) were the most prevalent in WNV. ORs of severe flavivirus diseases were about 2 to 4 in infected patients with comorbidities such as diabetes, hypertension and heart diseases. The small number of studies in JEV, YFV and Zika did not permit estimating the prevalence of comorbidities in these infections. CONCLUSION: Higher prevalence of chronic comorbidities was found in severe cases of flavivirus diseases compared to non-severe cases. Findings of the present study may guide public health practitioners and clinicians to evaluate infection severity based on the presence of comorbidity, a critical public health measure that may avert severe disease outcome given the current dearth of clear prevention practices for some flavivirus diseases. url: https://doi.org/10.1371/journal.pone.0200200 doi: 10.1371/journal.pone.0200200 id: cord-002413-795wuqz5 author: Balinsky, Corey A. title: IRAV (FLJ11286), an Interferon-Stimulated Gene with Antiviral Activity against Dengue Virus, Interacts with MOV10 date: 2017-02-14 words: 5485.0 sentences: 278.0 pages: flesch: 47.0 cache: ./cache/cord-002413-795wuqz5.txt txt: ./txt/cord-002413-795wuqz5.txt summary: title: IRAV (FLJ11286), an Interferon-Stimulated Gene with Antiviral Activity against Dengue Virus, Interacts with MOV10 IRAV is an RNA binding protein and localizes to cytoplasmic processing bodies (P bodies) in uninfected cells, where it interacts with the MOV10 RISC complex RNA helicase, suggesting a role for IRAV in the processing of viral RNA. FLJ11286, which we refer to here as IRAV (interferon-regulated antiviral gene) (also annotated as C19orf66, UPF0515, or RyDEN), encodes a protein 291 amino acids (aa) in length with a calculated molecular mass of 33.1 kDa. Analysis of published microarray data suggests that IRAV (FLJ11286) is upregulated in response to type I and type II IFNs (6, 20, (24) (25) (26) . IRAV also associates with the host RNA binding proteins UPF1 and HuR (ELAV1) and interacts with MOV10 (a RISC complex RNA helicase), suggesting a role for IRAV in processing or stability of RNA. abstract: Dengue virus (DENV) is a member of the genus Flavivirus and can cause severe febrile illness. Here, we show that FLJ11286, which we refer to as IRAV, is induced by DENV in an interferon-dependent manner, displays antiviral activity against DENV, and localizes to the DENV replication complex. IRAV is an RNA binding protein and localizes to cytoplasmic processing bodies (P bodies) in uninfected cells, where it interacts with the MOV10 RISC complex RNA helicase, suggesting a role for IRAV in the processing of viral RNA. After DENV infection, IRAV, along with MOV10 and Xrn1, localizes to the DENV replication complex and associates with DENV proteins. Depletion of IRAV or MOV10 results in an increase in viral RNA. These data serve to characterize an interferon-stimulated gene with antiviral activity against DENV, as well as to propose a mechanism of activity involving the processing of viral RNA. IMPORTANCE Dengue virus, a member of the family Flaviviridae, can result in a life-threatening illness and has a significant impact on global health. Dengue virus has been shown to be particularly sensitive to the effects of type I interferon; however, little is known about the mechanisms by which interferon-stimulated genes function to inhibit viral replication. A better understanding of the interferon-mediated antiviral response to dengue virus may aid in the development of novel therapeutics. Here, we examine the influence of the interferon-stimulated gene IRAV (FLJ11286) on dengue virus replication. We show that IRAV associates with P bodies in uninfected cells and with the dengue virus replication complex after infection. IRAV also interacts with MOV10, depletion of which is associated with increased viral replication. Our results provide insight into a newly identified antiviral gene, as well as broadening our understanding of the innate immune response to dengue virus infection. url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5309953/ doi: 10.1128/jvi.01606-16 id: cord-290385-0smnl70i author: Chan, Jasper F.W. title: Zika fever and congenital Zika syndrome: An unexpected emerging arboviral disease date: 2016-03-03 words: nan sentences: nan pages: flesch: nan cache: txt: summary: abstract: Unlike its mosquito-borne relatives, such as dengue, West Nile, and Japanese encephalitis viruses, which can cause severe human diseases, Zika virus (ZIKV) has emerged from obscurity by its association with a suspected “congenital Zika syndrome”, while causing asymptomatic or mild exanthematous febrile infections which are dengue- or rubella-like in infected individuals. Despite having been discovered in Uganda for almost 60 years, <20 human cases were reported before 2007. The massive epidemics in the Pacific islands associated with the ZIKV Asian lineage in 2007 and 2013 were followed by explosive outbreaks in Latin America in 2015. Although increased mosquito breeding associated with the El Niño effect superimposed on global warming is suspected, genetic changes in its RNA virus genome may have led to better adaptation to mosquitoes, other animal reservoirs, and human. We reviewed the epidemiology, clinical manifestation, virology, pathogenesis, laboratory diagnosis, management, and prevention of this emerging infection. Laboratory diagnosis can be confounded by cross-reactivity with other circulating flaviviruses. Besides mosquito bite and transplacental transmission, the risk of other potential routes of transmission by transfusion, transplantation, sexual activity, breastfeeding, respiratory droplet, and animal bite is discussed. Epidemic control requires adequate clearance of mosquito breeding grounds, personal protection against mosquito bite, and hopefully a safe and effective vaccine. url: https://www.sciencedirect.com/science/article/pii/S016344531600061X doi: 10.1016/j.jinf.2016.02.011 id: cord-303818-z3js3mr4 author: Chen, Huixin title: Development and Evaluation of a SYBR Green–Based Real-Time Multiplex RT-PCR Assay for Simultaneous Detection and Serotyping of Dengue and Chikungunya Viruses date: 2015-10-11 words: nan sentences: nan pages: flesch: nan cache: txt: summary: abstract: Chikungunya virus (CHIKV) and dengue virus (DENV) have emerged as the two most important arbovirus diseases of global health significance. Similar clinical manifestations, transmission vectors, geographical distribution, and seasonal correlation often result in misdiagnosis of chikungunya infections as dengue cases and vice versa. In this study, we developed a rapid and accurate laboratory confirmative method to simultaneously detect, quantify, and differentiate DENV serotypes 1, 2, 3, and 4 and CHIKV. This SYBR Green I–based one-step multiplex real-time RT-PCR assay is highly sensitive and specific for CHIKV and DENV. Melting temperature analysis of PCR amplicons was used to serotype DENV and to differentiate from CHIKV. The detection limit of the assay was 20, 10, 50, 5, and 10 RNA copies/reaction for DENV-1, DENV-2, DENV-3, DENV-4, and CHIKV, respectively. Our assay did not cross-react with a panel of viruses that included other flaviviruses, alphaviruses, influenza viruses, human enteroviruses, and human coronaviruses. The feasibility of using this assay for clinical diagnosis was evaluated in DENV- and CHIKV-positive patient sera. Accordingly, the assay sensitivity for DENV-1, DENV-2, DENV-3, DENV-4, and CHIKV was 89.66%, 96.67%, 96.67%, 94.12%, and 95.74%, respectively, with 100% specificity. These findings confirmed the potential of our assay to be used as a rapid test for simultaneous detection and serotyping of DENV and CHIKV in clinical samples. url: https://www.sciencedirect.com/science/article/pii/S1525157815001567 doi: 10.1016/j.jmoldx.2015.06.008 id: cord-274129-vaygaqe5 author: Cheng, Ming Soon title: Impedimetric cell-based biosensor for real-time monitoring of cytopathic effects induced by dengue viruses date: 2015-08-15 words: nan sentences: nan pages: flesch: nan cache: txt: summary: abstract: We describe an impedimetric cell-based biosensor constructed from poly-l-lysine (PLL)-modified screen-printed carbon electrode for real-time monitoring of dengue virus (DENV) infection of surface-immobilized baby hamster kidney (BHK-21) fibroblast cells. Cytopathic effects (CPE) induced by DENV-2 New Guinea C strain (including degenerative morphological changes, detachment, membrane degradation and death of host cells), were reflected by drastic decrease in impedance signal response detected as early as ~30 hours post-infection (hpi). In contrast, distinct CPE by conventional microscopy was evident only at ~72 hpi at the corresponding multiplicity of infection (MOI) of 10. A parameter that describes the kinetics of cytopathogenesis, CIT(50), which refers to the time taken for 50% reduction in impedance signal response, revealed an inverse linear relationship with virus titer and MOI. CIT(50) values were also delayed by 31.5 h for each order of magnitude decrease in MOI. Therefore, based on the analysis of CIT(50), the virus titer of a given sample can be determined from the measured impedance signal response. Furthermore, consistent impedance results were also obtained with clinical isolates of the four DENV serotypes verified by RT-PCR and cycle sequencing. This impedimetric cell-based biosensor represents a label-free and continuous approach for the dynamic measurement of cellular responses toward DENV infection, and for detecting the presence of infectious viral particles. url: https://doi.org/10.1016/j.bios.2015.03.018 doi: 10.1016/j.bios.2015.03.018 id: cord-317169-qlqavi4t author: Chiow, K.H. title: Evaluation of antiviral activities of Houttuynia cordata Thunb. extract, quercetin, quercetrin and cinanserin on murine coronavirus and dengue virus infection date: 2015-12-19 words: 4302.0 sentences: 225.0 pages: flesch: 50.0 cache: ./cache/cord-317169-qlqavi4t.txt txt: ./txt/cord-317169-qlqavi4t.txt summary: (Saururaceae) and three of its constituent flavonoids (quercetin, quercitrin and rutin) against murine coronavirus and dengue virus (DENV). cordata and flavonoids were assessed using virus neutralization tests against mouse hepatitis virus (MHV) and DENV type 2 (DENV-2). Certain flavonoids exhibited comparatively weaker antiviral activity, notably quercetin which could inhibit both MHV and DENV-2. This was followed by quercitrin which could inhibit DENV-2 but not MHV, whereas rutin did not exert any inhibitory effect on either virus. cordata contribute to the superior antiviral efficacy of the EA fraction which lacked cytotoxicity in vitro and acute toxicity in vivo. cordata exhibited anti-MHV activity at a MIC of 0.98 mg/mL without any apparent cytotoxic effects on CCL9.1 cells. cordata and its flavonoid component, quercetin, could inhibit both MHV and DENV-2 in vitro. Houttuynia cordata extracts and constituents inhibit the infectivity of dengue virus type 2 in vitro abstract: OBJECTIVE: To evaluate the in vitro activities of the ethyl acetate (EA) fraction of Houttuynia cordata (H. cordata) Thunb. (Saururaceae) and three of its constituent flavonoids (quercetin, quercitrin and rutin) against murine coronavirus and dengue virus (DENV). METHODS: The antiviral activities of various concentrations of the EA fraction of H. cordata and flavonoids were assessed using virus neutralization tests against mouse hepatitis virus (MHV) and DENV type 2 (DENV-2). Cinanserin hydrochloride was also tested against MHV. The EA fraction of H. cordata was tested for acute oral toxicity in C57BL/6 mice. RESULTS: The EA fraction of H. cordata inhibited viral infectivity up to 6 d. Cinanserin hydrochloride was able to inhibit MHV for only 2 d. The 50% inhibitory concentrations (IC(50)) of the EA fraction of H. cordata added before the viral adsorption stage were 0.98 μg/mL for MHV and 7.50 μg/mL for DENV-2 with absence of cytotoxicity. The mice fed with the EA fraction up to 2 000 mg/kg did not induce any signs of acute toxicity, with normal histological features of major organs. Certain flavonoids exhibited comparatively weaker antiviral activity, notably quercetin which could inhibit both MHV and DENV-2. This was followed by quercitrin which could inhibit DENV-2 but not MHV, whereas rutin did not exert any inhibitory effect on either virus. When quercetin was combined with quercitrin, enhancement of anti-DENV-2 activity and reduced cytotoxicity were observed. However, the synergistic efficacy of the flavonoid combination was still less than that of the EA fraction. CONCLUSIONS: The compounds in H. cordata contribute to the superior antiviral efficacy of the EA fraction which lacked cytotoxicity in vitro and acute toxicity in vivo. H. cordata has much potential for the development of antiviral agents against coronavirus and dengue infections. url: https://www.ncbi.nlm.nih.gov/pubmed/26851778/ doi: 10.1016/j.apjtm.2015.12.002 id: cord-011390-98xyie7s author: Darcy, Andrew Waleluma title: Multiple arboviral infections during a DENV-2 outbreak in Solomon Islands date: 2020-05-15 words: 4538.0 sentences: 236.0 pages: flesch: 53.0 cache: ./cache/cord-011390-98xyie7s.txt txt: ./txt/cord-011390-98xyie7s.txt summary: An outbreak of dengue-like illness (DLI) that occurred in April 2016 prompted this study, which aimed to determine the population''s immunity status and identify the arboviruses circulating in the country. The study objectives were to estimate the seroprevalence of arboviruses in the urban population of Solomon Islands while using qPCR to determine the circulating viruses from the clinical samples; furthermore, to characterize and compare the dengue virus isolated from our study to previous published isolates from the region using the phylogenetic tree analysis. The high positivity rate for anti-DENV IgG antibodies (77.9%) and the high DENV positivity rate detected in the qPCR analysis of the clinical samples (76.3%) were indicative of a high number of secondary infections in the 2016 outbreak. The phylogenetic tree analysis and the serotypespecific qPCR results indicated that there was a single DENV-3 serotype in circulation from 2012 to 2016 in Solomon Islands. abstract: BACKGROUND: Solomon Islands, a country made up of tropical islands, has suffered cyclic dengue fever (DF) outbreaks in the past three decades. An outbreak of dengue-like illness (DLI) that occurred in April 2016 prompted this study, which aimed to determine the population’s immunity status and identify the arboviruses circulating in the country. METHODS: A household survey, involving 188 participants in two urban areas (Honiara and Gizo), and a parallel hospital-based clinical survey were conducted in April 2016. The latter was repeated in December after a surge in DLI cases. Arbovirus IgG ELISA were performed on the household blood samples to determine the prevalence of arboviruses in the community, while qPCR testing of the clinical samples was used to identify the circulating arboviruses. Dengue virus (DENV)-positive samples were further characterized by amplifying and sequencing the envelope gene. RESULTS: The overall prevalence rates of DENV, Zika virus, and chikungunya virus were 83.4%, 7.6%, and 0.9%, respectively. The qPCR positivity rates of the clinical samples collected in April 2016 were as follows: DENV 39.6%, Zika virus 16.7%, and chikungunya virus 6.3%, which increased to 74%, 48%, and 20% respectively in December 2016. The displacement of the circulating serotype-3, genotype-1, with DENV serotype 2, genotype cosmopolitan was responsible for the outbreak in 2016. CONCLUSIONS: A DENV outbreak in Solomon Islands was caused by the introduction of a single serotype. The high prevalence of DENV provided transient cross-protection, which prevented the introduction of a new serotype from the hyperendemic region for at least 3 years. The severe outcomes seen in the recent outbreak probably resulted from changes in the causative viruses and the effects of population immunity and changes in the outbreak pattern. Solomon Islands needs to step up surveillance to include molecular tools, increase regional communication, and perform timely interventions. url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7225641/ doi: 10.1186/s41182-020-00217-8 id: cord-318120-vfznyyz6 author: Dauner, Allison L. title: Development of a pan-serotype reverse transcription loop-mediated isothermal amplification assay for the detection of dengue virus date: 2015-05-15 words: 5265.0 sentences: 288.0 pages: flesch: 48.0 cache: ./cache/cord-318120-vfznyyz6.txt txt: ./txt/cord-318120-vfznyyz6.txt summary: title: Development of a pan-serotype reverse transcription loop-mediated isothermal amplification assay for the detection of dengue virus To facilitate needed diagnosis, we developed and optimized a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay that detects all 4 serotypes of dengue virus (DENV). Development of simple and rapid assay to detect viral RNA of tick-borne encephalitis virus by reverse transcription-loop-mediated isothermal amplification A real-time reverse transcription loop-mediated isothermal amplification assay for the rapid detection of yellow fever virus Rapid detection and differentiation of dengue virus serotypes by NS1 specific reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay in patients presenting to a tertiary care hospital in Rapid detection and differentiation of dengue virus serotypes by a real-time reverse transcription-loopmediated isothermal amplification assay Development and evaluation of reverse transcription-loop-mediated isothermal amplification assay for rapid and real-time detection of Japanese encephalitis virus abstract: During dengue outbreaks, acute diagnosis at the patient's point of need followed by appropriate supportive therapy reduces morbidity and mortality. To facilitate needed diagnosis, we developed and optimized a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay that detects all 4 serotypes of dengue virus (DENV). We used a quencher to reduce nonspecific amplification. The assay does not require expensive thermocyclers, utilizing a simple water bath to maintain the reaction at 63 °C. Results can be visualized using UV fluorescence, handheld readers, or lateral flow immunochromatographic tests. We report a sensitivity of 86.3% (95% confidence interval [CI], 72.7–94.8%) and specificity of 93.0% (95% CI, 83.0–98.1%) using a panel of clinical specimens characterized by DENV quantitative reverse transcription–polymerase chain reaction. This pan-serotype DENV RT-LAMP can be adapted to field-expedient formats where it can provide actionable diagnosis near the patient's point of need. url: https://www.sciencedirect.com/science/article/pii/S0732889315001571 doi: 10.1016/j.diagmicrobio.2015.05.004 id: cord-282742-eyukbot7 author: Diosa-Toro, Mayra title: Arthropod-Borne Flaviviruses and RNA Interference: Seeking New Approaches for Antiviral Therapy date: 2013-02-20 words: 6493.0 sentences: 364.0 pages: flesch: 48.0 cache: ./cache/cord-282742-eyukbot7.txt txt: ./txt/cord-282742-eyukbot7.txt summary: Geiss, Pierson, and Diamond (2005) observed that siRNAs targeting the C gene had no effect on virus replication when transfected into cells 10 h after WNV infection using lipid-based reagents. In addition, no significant reduction in viral protein or RNA levels was seen in WNV replicon-expressing cells transfected with siRNAs targeting the NS3 gene using lipid-based reagents. Also, a recent report showed that siRNA toward the TNF-a gene reduced cytokine response in DENV-infected DCs, highlighting the potential of targeted RNAi-based approaches to simultaneously decrease viral replication and the detrimental host immune response (Subramanya et al., 2010) . In addition, it has been shown that WNV (Chotkowski et al., 2008) and DENV (Mukherjee & Hanley, 2010) infection (Mukherjee & Hanley, 2010) of Drosophila cell lines induce functional virus-specific siRNAs that promote a protective RNAi response. So far we have described the antiviral effect of the RNAi mechanism induced by exogenous delivery of siRNA or precursors, and how cellular miRNA can target sequences artificially introduced within the genome of flaviviruses. abstract: Flaviviruses are the most prevalent arthropod-borne viruses worldwide, and nearly half of the 70 Flavivirus members identified are human pathogens. Despite the huge clinical impact of flaviviruses, there is no specific human antiviral therapy available to treat infection with any of the flaviviruses. Therefore, there is a continued search for novel therapies, and this review describes the current knowledge on the usage of RNA interference (RNAi) in combating flavivirus infections. RNAi is a process of sequence-specific gene silencing triggered by double-stranded RNA. Antiviral RNAi strategies against arthropod-borne flaviviruses have been reported and although several hurdles must be overcome to employ this technology in clinical applications, they potentially represent a new therapeutic tool. url: https://www.sciencedirect.com/science/article/pii/B9780124081161000045 doi: 10.1016/b978-0-12-408116-1.00004-5 id: cord-011026-iapgkz0p author: El-Bitar, Alaa M. H. title: Smp76, a Scorpine-Like Peptide Isolated from the Venom of the Scorpion Scorpio maurus palmatus, with a Potent Antiviral Activity Against Hepatitis C Virus and Dengue Virus date: 2019-07-06 words: 5247.0 sentences: 296.0 pages: flesch: 53.0 cache: ./cache/cord-011026-iapgkz0p.txt txt: ./txt/cord-011026-iapgkz0p.txt summary: title: Smp76, a Scorpine-Like Peptide Isolated from the Venom of the Scorpion Scorpio maurus palmatus, with a Potent Antiviral Activity Against Hepatitis C Virus and Dengue Virus Smp76 antiviral activity was evaluated using a cell culture technique utilizing Huh7it-1, Vero/SLAM, HCV (JFH1, genotype 2a) and DENV (Trinidad 1751, type 2). For dengue virus infectivity, serially diluted venom fractions and Smp76 were mixed with fixed amount of DENV and incubated for 2 h at 37 °C. The above results suggest that the Smp76 directly affects HCV particles and/or host cells in the culture medium to inhibit the viral infection and does not have an antiviral effect in the cells. To determine whether the antiviral activity of Smp76 peptide (previously described) was specific to HCV and DENV, a Schematic of infection assay. The exact mechanism by which Smp76 exerts its antiviral activity against HCV and DENV to inhibit infecting their target cells need further studies. abstract: Growing global viral infections have been a serious public health problem in recent years. This current situation emphasizes the importance of developing more therapeutic antiviral compounds. Hepatitis C virus (HCV) and dengue virus (DENV) belong to the Flaviviridae family and are an increasing global health threat. Our previous study reported that the crude venom of Scorpio maurus palmatus possessed anti-HCV and anti-DENV activities in vitro. We report here the characterization of a natural antiviral peptide (scorpion-like peptide Smp76) that prevents HCV and DENV infection. Smp76 was purified from S. m. palmatus venom and contains 76 amino acids with six residues of cysteine. Smp76 antiviral activity was evaluated using a cell culture technique utilizing Huh7it-1, Vero/SLAM, HCV (JFH1, genotype 2a) and DENV (Trinidad 1751, type 2). A potential antiviral activity of Smp76 was detected in culture cells with an approximate IC(50) of 0.01 μg/ml. Moreover, Smp76 prevents HCV infection and suppresses secondary infection, by inactivating extra-cellular infectious particles without affecting viral replication. Interestingly, Smp76 is neither toxic nor hemolytic in vitro at a concentration 1000-fold higher than that required for antiviral activity. Conclusively, this report highlights novel anti-HCV and anti-DENV activities of Smp76, which may lay the foundation for developing a new therapeutic intervention against these flaviviruses. url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7223391/ doi: 10.1007/s10989-019-09888-2 id: cord-002426-5e1xn7kj author: Falcón-Lezama, Jorge Abelardo title: Analysis of spatial mobility in subjects from a Dengue endemic urban locality in Morelos State, Mexico date: 2017-02-22 words: 5944.0 sentences: 261.0 pages: flesch: 49.0 cache: ./cache/cord-002426-5e1xn7kj.txt txt: ./txt/cord-002426-5e1xn7kj.txt summary: MATERIALS AND METHODS: We carried out a cohort-nested, case-control study with 126 individuals (42 cases, 42 intradomestic controls and 42 population controls) with the goal of describing human mobility patterns of recently Dengue virus-infected subjects, and comparing them with those of non-infected subjects living in an urban endemic locality. CONCLUSIONS: Results of this study show that human mobility in a small urban setting exceeded that considered by local health authority''s administrative limits, and was different between recently infected and non-infected subjects living in the same household. These observations provide important insights about the role that human mobility may have in Dengue virus transmission and persistence across endemic geographic areas that need to be taken into account when planning preventive and control measures. Sample: 126 individuals (42 cases, 42 intradomestic controls and 42 population controls) with age older than 12, and residents in Axochiapan, Morelos State, México, were selected from the cohort "Peridomestic infection as determinant for Dengue virus transmission" [13] . abstract: INTRODUCTION: Mathematical models and field data suggest that human mobility is an important driver for Dengue virus transmission. Nonetheless little is known on this matter due the lack of instruments for precise mobility quantification and study design difficulties. MATERIALS AND METHODS: We carried out a cohort-nested, case-control study with 126 individuals (42 cases, 42 intradomestic controls and 42 population controls) with the goal of describing human mobility patterns of recently Dengue virus-infected subjects, and comparing them with those of non-infected subjects living in an urban endemic locality. Mobility was quantified using a GPS-data logger registering waypoints at 60-second intervals for a minimum of 15 natural days. RESULTS: Although absolute displacement was highly biased towards the intradomestic and peridomestic areas, occasional displacements exceeding a 100-Km radius from the center of the studied locality were recorded for all three study groups and individual displacements were recorded traveling across six states from central Mexico. Additionally, cases had a larger number of visits out of the municipality´s administrative limits when compared to intradomestic controls (cases: 10.4 versus intradomestic controls: 2.9, p = 0.0282). We were able to identify extradomestic places within and out of the locality that were independently visited by apparently non-related infected subjects, consistent with houses, working and leisure places. CONCLUSIONS: Results of this study show that human mobility in a small urban setting exceeded that considered by local health authority’s administrative limits, and was different between recently infected and non-infected subjects living in the same household. These observations provide important insights about the role that human mobility may have in Dengue virus transmission and persistence across endemic geographic areas that need to be taken into account when planning preventive and control measures. Finally, these results are a valuable reference when setting the parameters for future mathematical modeling studies. url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5321279/ doi: 10.1371/journal.pone.0172313 id: cord-273065-peqz7okh author: Girard, Marc title: Arboviruses: A global public health threat date: 2020-04-24 words: nan sentences: nan pages: flesch: nan cache: txt: summary: abstract: A conference on «ARBOVIRUSES, A GLOBAL PUBLIC HEALTH THREAT» was organized on June 20–22, 2018 at the Merieux Foundation Conference Center in Veyrier du Lac, France, to review and raise awareness to the global public health threat of epidemic arboviruses, and to advance the discussion on the control and prevention of arboviral diseases. The presentations by scientists and public health officials from Asia, the Americas, Europe and Africa strengthened the notion that arboviral diseases of both humans and domestic animals are progressively becoming dominant public health problems in the world. The repeated occurrence of recent deadly epidemics strongly reinforces the call for action against these viral diseases, and the need for developing effective vaccines, drugs, vector control tools and strong prevention programs. url: https://www.sciencedirect.com/science/article/pii/S0264410X20304709 doi: 10.1016/j.vaccine.2020.04.011 id: cord-278260-3o91v72a author: Halstead, Scott B title: COVID 19 Vaccines: Should we fear ADE? date: 2020-08-12 words: 2331.0 sentences: 178.0 pages: flesch: 44.0 cache: ./cache/cord-278260-3o91v72a.txt txt: ./txt/cord-278260-3o91v72a.txt summary: Within months large numbers of vaccinated children developed a severe breakthrough disease, called "atypical measles." [6] A similar outcome, "vaccine associated enhanced respiratory disease (VAERD)," was observed in infants, 4 -12 months of age, who were given formalininactivated respiratory syncytial virus (RSV) and a few months later infected by RSV. The biological behavior of some coronaviruses in non-human species together with evidence that human coronavirus antibodies enhanced infection of SARS or MERS CoVs in Fc receptor-bearing cells, in vitro, have led to speculations that ADE contributes to disease severity in humans. [11] It has been reported that high levels of SARS CoV-1 IgG antibodies circulated in severe SARS cases and that anti-S IgG neutralizing antibody (NAb) responses developed significantly faster after the onset of clinical symptoms in fatal compared with recovered cases leading some to attribute enhanced tissue damage to ADE. With others, we conclude that the differences in clinical, epidemiological and pathological features of SARS and DENV diseases suggest that iADE does not contribute to the severity of natural human coronavirus infections. abstract: Might COVID 19 vaccines sensitize humans to antibody dependent enhanced (ADE) breakthrough infections? This outcome is unlikely because coronavirus diseases in humans lack the clinical, epidemiological, biological or pathological attributes of ADE disease exemplified by the dengue viruses (DENV). In contrast to DENV, SARS and MERS CoVs predominantly infect respiratory epithelium, not macrophages. Severe disease centers on older persons with pre-existing conditions and not young infants or individuals with previous coronavirus infections. Live virus challenge of animals given SARS or MERS vaccines has resulted in vaccine hypersensitivity reactions (VAH), similar to those in humans given inactivated measles or respiratory syncytial virus vaccines. Safe and effective COVID 19 vaccines must avoid VAH. url: https://www.ncbi.nlm.nih.gov/pubmed/32785649/ doi: 10.1093/infdis/jiaa518 id: cord-285538-3ah05ijf author: Halstead, Scott B. title: Chapter 19 Pathogenic Exploitation of Fc Activity date: 2014-12-31 words: 7637.0 sentences: 426.0 pages: flesch: 39.0 cache: ./cache/cord-285538-3ah05ijf.txt txt: ./txt/cord-285538-3ah05ijf.txt summary: 3, 4 While follow-up research in the 1960s suggested that this phenomenon resulted from the stabilization of MVEV by antibodies, 5 a different explanation emerged when sequential infections in humans with dengue viruses (DENVs) were shown to produce severe disease (dengue hemorrhagic fever, DHF). Unexpectedly, the incubation of Ross River virus (RRV) with diluted RRV antiserum resulted in enhanced infection in mouse macrophage cell lines and in primary human monocytes/macrophages via innate immune suppression involving reduced production of reactive nitrogen radicals via NOS2 and a downregulation of TNF-α and IFN-β production through abolished interferon regulatory factor 1 (IRF-1) and nuclear factor-κB gene expression. 43 Note has been made above of the possible role of different Fcγ receptors in mediating ADE in macrophages in leishmania mouse models and the interesting observation suggesting that ligation of FcγRIIB may affect the fate of large immune complexes during the immune clearance phase of dengue infections. abstract: While the benefits of antibody responses are widely known, pathogens are also able to exploit antibodies to facilitate cell entry and potentially alter the cellular response via interactions with Fc receptors. This phenomenon, known as antibody-dependent enhancement (ADE) of disease, is a factor in numerous human and veterinary diseases. It is thought to result from innate cellular responses to Fcγ receptor-facilitated entry of infectious microbial immune complexes, and paradoxically results in increased production of pathogenic organisms. ADE has been described in vitro in numerous settings, but the strongest data regarding the in vivo impact of this mechanism on human disease come from human disease and animal models of dengue and leishmanial infections. This chapter reviews the literature of ADE in relation to the innate immune responses to Fcγ receptor ligation by infectious IgG immune complexes and discusses the research frontiers regarding this harmful antibody activity. url: https://api.elsevier.com/content/article/pii/B9780123948021000194 doi: 10.1016/b978-0-12-394802-1.00019-4 id: cord-273388-615acz0l author: He, Miao title: The Impact of Emerging Infectious Diseases on Chinese Blood Safety() date: 2016-11-04 words: nan sentences: nan pages: flesch: nan cache: txt: summary: abstract: Emerging infectious diseases (EIDs) have always been one of the major threats to public health. Although the implementation of mandatory testing for 4 classical transfusion-transmitted infectious—human immunodeficiency virus, hepatitis B virus, hepatitis C virus, and syphilis—has reduced the transfusion risk of these pathogens, the potential threat of various EID agents and their constantly evolving variants to blood safety in China is not fully understood. This review presents 9 representative EID agents that are autochthonous and epidemic nationally or regionally in China. The epidemiologic status and distribution of these EID agents among donors and/or healthy populations are summarized. The potential risks of these EID agents to blood safety are discussed. The review also explores strategies to strengthen hemovigilance systems and studies to further evaluate the impact of EID agents on blood safety. url: https://www.ncbi.nlm.nih.gov/pubmed/27923518/ doi: 10.1016/j.tmrv.2016.10.002 id: cord-272459-w14finxf author: Heaton, Nicholas S. title: Dengue Virus and Autophagy date: 2011-08-04 words: 3107.0 sentences: 182.0 pages: flesch: 40.0 cache: ./cache/cord-272459-w14finxf.txt txt: ./txt/cord-272459-w14finxf.txt summary: Recently, it was reported that autophagy plays an indirect role in DENV replication by modulating cellular lipid metabolism. Relevant to DENV infection, a type of selective autophagy termed lipophagy was described, wherein autophagosomes can target cellular stores of lipids known as lipid droplets (LDs) to generate energy for the cell [38] . In subsequent work, the authors reproduced the published results that DENV induces and requires autophagy for robust viral replication [39] . These autophagosomes did not co-localize with markers of the viral replication complex, suggesting that they may play an indirect, non-structural role in DENV replication. Alternatively, DENV infection induces a selective autophagy that is preferentially targeted to lipid droplets, which leads to changes in cellular metabolism. More work, however, is required to show whether the proposed viral triggers of autophagy reproduce all cellular signals and phenotypes that accompany autophagy induction in DENV-infected cells. abstract: Several independent groups have published that autophagy is required for optimal RNA replication of dengue virus (DENV). Initially, it was postulated that autophagosomes might play a structural role in replication complex formation. However, cryo-EM tomography of DENV replication complexes showed that DENV replicates on endoplasmic reticulum (ER) cisternae invaginations and not on classical autophagosomes. Recently, it was reported that autophagy plays an indirect role in DENV replication by modulating cellular lipid metabolism. DENV-induced autophagosomes deplete cellular triglycerides that are stored in lipid droplets, leading to increased β-oxidation and energy production. This is the first example of a virus triggering autophagy to modulate cellular physiology. In this review, we summarize these data and discuss new questions and implications for autophagy during DENV replication. url: https://www.ncbi.nlm.nih.gov/pubmed/21994782/ doi: 10.3390/v3081332 id: cord-355610-7xy4s483 author: Hu, Dan title: A broadly neutralizing germline-like human monoclonal antibody against dengue virus envelope domain III date: 2019-06-26 words: nan sentences: nan pages: flesch: nan cache: txt: summary: abstract: Dengue is the most widespread vector-borne viral disease caused by dengue virus (DENV) for which there are no safe, effective drugs approved for clinical use. Here, by using sequential antigen panning of a yeast antibody library derived from healthy donors against the DENV envelop protein domain III (DIII) combined with depletion by an entry defective DIII mutant, we identified a cross-reactive human monoclonal antibody (mAb), m366.6, which bound with high affinity to DENV DIII from all four DENV serotypes. Immunogenetic analysis indicated that m366.6 is a germline-like mAb with very few somatic mutations from the closest VH and Vλ germline genes. Importantly, we demonstrated that it potently neutralized DENV both in vitro and in the mouse models of DENV infection without detectable antibody-dependent enhancement (ADE) effect. The epitope of m366.6 was mapped to the highly conserved regions on DIII, which may guide the design of effective dengue vaccine immunogens. Furthermore, as the first germline-like mAb derived from a naïve antibody library that could neutralize all four DENV serotypes, the m366.6 can be a tool for exploring mechanisms of DENV infection, and is a promising therapeutic candidate. url: https://www.ncbi.nlm.nih.gov/pubmed/31242272/ doi: 10.1371/journal.ppat.1007836 id: cord-355179-wmfwl2bh author: Jung, Eunhye title: Neutralization of Acidic Intracellular Vesicles by Niclosamide Inhibits Multiple Steps of the Dengue Virus Life Cycle In Vitro date: 2019-06-18 words: 5305.0 sentences: 271.0 pages: flesch: 44.0 cache: ./cache/cord-355179-wmfwl2bh.txt txt: ./txt/cord-355179-wmfwl2bh.txt summary: Overall, the data indicate that the antiviral activity of niclosamide during the early stage of the DENV life cycle correlates with the neutralization profile of the low-pH compartments, suggesting that blocking endosomal acidification results in the inhibition of viral genome replication and polyprotein processing, which further impedes viral protein expression and virus production. In this study, we found that neutralization of low-pH intracellular compartments by niclosamide not only inhibited the early stage of the DENV viral life cycle, such as viral RNA replication, independent of the entry step but also the late stage, specifically, the maturation of virus particles into infectious virions. Indeed, niclosamide treatment www.nature.com/scientificreports www.nature.com/scientificreports/ during the first 6 h of infection reduced DENV replication in BHK-21 cells harbouring dengue replicons to a level comparable to that of ribavirin, suggesting that the drug affects viral RNA replication and/or translation independent of its effect on entry, membrane fusion and genome release. abstract: Dengue fever is one of the most important mosquito-borne viral infections in large parts of tropical and subtropical countries and is a significant public health concern and socioeconomic burden. There is an urgent need to develop antivirals that can effectively reduce dengue virus (DENV) replication and decrease viral load. Niclosamide, an antiparasitic drug approved for human use, has been recently identified as an effective antiviral agent against a number of pH-dependent viruses, including flaviviruses. Here, we reveal that neutralization of low-pH intracellular compartments by niclosamide affects multiple steps of the DENV infectious cycle. Specifically, niclosamide-induced endosomal neutralization not only prevents viral RNA replication but also affects the maturation of DENV particles, rendering them non-infectious. We found that niclosamide-induced endosomal neutralization prevented E glycoprotein conformational changes on the virion surface of flaviviruses, resulting in the release of non-infectious immature virus particles with uncleaved pr peptide from host cells. Collectively, our findings support the potential application of niclosamide as an antiviral agent against flavivirus infection and highlight a previously uncharacterized mechanism of action of the drug. url: https://www.ncbi.nlm.nih.gov/pubmed/31213630/ doi: 10.1038/s41598-019-45095-1 id: cord-342157-qjyooq68 author: King, Chwan-Chuen title: Comparative analysis of full genomic sequences among different genotypes of dengue virus type 3 date: 2008-05-21 words: 5860.0 sentences: 277.0 pages: flesch: 48.0 cache: ./cache/cord-342157-qjyooq68.txt txt: ./txt/cord-342157-qjyooq68.txt summary: In our study, complete genomic sequencing of DENV-3 strains collected from different geographical locations and isolation years were determined and the sequence diversity as well as selection pressure sites in the DENV genome other than within the E gene were also analyzed. RESULTS: Using maximum likelihood and Bayesian approaches, our phylogenetic analysis revealed that the Taiwan''s indigenous DENV-3 isolated from 1994 and 1998 dengue/DHF epidemics and one 1999 sporadic case were of the three different genotypes – I, II, and III, each associated with DENV-3 circulating in Indonesia, Thailand and Sri Lanka, respectively. Compared to the prototype strain H87, several unique amino acid substitutions that serve as unique signature sites for each genotype were found within the full genomic sequences of the selected DENV-3 isolates from Taiwan or other countries and are listed by the order of the gene in Table 3 . abstract: BACKGROUND: Although the previous study demonstrated the envelope protein of dengue viruses is under purifying selection pressure, little is known about the genetic differences of full-length viral genomes of DENV-3. In our study, complete genomic sequencing of DENV-3 strains collected from different geographical locations and isolation years were determined and the sequence diversity as well as selection pressure sites in the DENV genome other than within the E gene were also analyzed. RESULTS: Using maximum likelihood and Bayesian approaches, our phylogenetic analysis revealed that the Taiwan's indigenous DENV-3 isolated from 1994 and 1998 dengue/DHF epidemics and one 1999 sporadic case were of the three different genotypes – I, II, and III, each associated with DENV-3 circulating in Indonesia, Thailand and Sri Lanka, respectively. Sequence diversity and selection pressure of different genomic regions among DENV-3 different genotypes was further examined to understand the global DENV-3 evolution. The highest nucleotide sequence diversity among the fully sequenced DENV-3 strains was found in the nonstructural protein 2A (mean ± SD: 5.84 ± 0.54) and envelope protein gene regions (mean ± SD: 5.04 ± 0.32). Further analysis found that positive selection pressure of DENV-3 may occur in the non-structural protein 1 gene region and the positive selection site was detected at position 178 of the NS1 gene. CONCLUSION: Our study confirmed that the envelope protein is under purifying selection pressure although it presented higher sequence diversity. The detection of positive selection pressure in the non-structural protein along genotype II indicated that DENV-3 originated from Southeast Asia needs to monitor the emergence of DENV strains with epidemic potential for better epidemic prevention and vaccine development. url: https://www.ncbi.nlm.nih.gov/pubmed/18495043/ doi: 10.1186/1743-422x-5-63 id: cord-011968-abd891ej author: Lai, Yen-Chung title: Roles of Macrophage Migration Inhibitory Factor in Dengue Pathogenesis: From Pathogenic Factor to Therapeutic Target date: 2020-06-12 words: 5705.0 sentences: 268.0 pages: flesch: 38.0 cache: ./cache/cord-011968-abd891ej.txt txt: ./txt/cord-011968-abd891ej.txt summary: Macrophage migration inhibitory factor (MIF) is a pleiotropic proinflammatory cytokine that mediates diverse immune responses, and the serum level of MIF positively correlates with disease severity in patients with dengue. In another study, it was demonstrated that live DENV2-induced endoplasmic reticulum (ER) stress is required for autophagy activation, viral replication and pathogenesis in HuH-7 and A549 cells [57] . In our study, since UV-inactivated viral particles could not induce MIF secretion or expression, it is possible that DENV infection triggered RNA sensing or pattern recognition receptor (PRR) activation, which was followed by the release of preformed MIF from the cytosol through the vesicle trafficking secretory pathway. Macrophage migration inhibitory factor induced by dengue virus infection increases vascular permeability Minocycline suppresses dengue virus replication by down-regulation of macrophage migration inhibitory factor-induced autophagy Dengue virus nonstructural protein 1 induces vascular leakage through macrophage migration inhibitory factor and autophagy abstract: Dengue virus (DENV) infection is the most prevalent mosquito-borne viral infection and can lead to severe dengue hemorrhagic fever (DHF) and even life-threatening dengue shock syndrome (DSS). Although the cytokine storm has been revealed as a critical factor in dengue disease, the limited understanding of dengue immunopathogenesis hinders the development of effective treatments. Macrophage migration inhibitory factor (MIF) is a pleiotropic proinflammatory cytokine that mediates diverse immune responses, and the serum level of MIF positively correlates with disease severity in patients with dengue. MIF is involved in DENV replication and many pathological changes, such as vascular leakage, during DENV infection. In this paper, the pathogenic roles of MIF and the regulation of MIF secretion during DENV infection are reviewed. Furthermore, whether MIF is a potential therapeutic target against DENV infection is also discussed. url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7356240/ doi: 10.3390/microorganisms8060891 id: cord-307219-okvvajms author: Lazzarini, Luca title: First autochthonous dengue outbreak in Italy, August 2020 date: 2020-09-10 words: 1887.0 sentences: 94.0 pages: flesch: 54.0 cache: ./cache/cord-307219-okvvajms.txt txt: ./txt/cord-307219-okvvajms.txt summary: In August 2020, during the coronavirus disease (COVID-19) pandemic, five locally acquired cases of dengue virus type 1 were detected in a family cluster in Vicenza Province, North-East Italy where Aedes albopictus mosquitoes are endemic. In August 2020, during the coronavirus disease (COVID-19) pandemic, five locally acquired cases of dengue virus type 1 were detected in a family cluster in Vicenza Province, North-East Italy where Aedes albopictus mosquitoes are endemic. She was investigated for West Nile virus, Usutu virus, dengue virus (DENV), chikungunya virus (CHIKV) and Zika virus (ZIKV) by molecular and serology testing, because she reported that a family member (Case 1) had had similar symptoms after a recent travel in Indonesia. The surveillance, active between June and November, aims to increase the detection rate of DENV and CHIKV (since 2015, also ZIKV) infection in travellers from endemic areas and to promptly identify potential autochthonous cases. abstract: In August 2020, during the coronavirus disease (COVID-19) pandemic, five locally acquired cases of dengue virus type 1 were detected in a family cluster in Vicenza Province, North-East Italy where Aedes albopictus mosquitoes are endemic. The primary case was an importation from West Sumatra, Indonesia. This is the first outbreak of autochthonous dengue reported in Italy. During the COVID-19 pandemic, screening of febrile travelers from endemic countries is crucial in areas where competent vectors are present. url: https://doi.org/10.2807/1560-7917.es.2020.25.36.2001606 doi: 10.2807/1560-7917.es.2020.25.36.2001606 id: cord-270495-2u072mtp author: Lokida, Dewi title: Diagnosis of COVID-19 in a Dengue-Endemic Area date: 2020-08-05 words: 1750.0 sentences: 110.0 pages: flesch: 53.0 cache: ./cache/cord-270495-2u072mtp.txt txt: ./txt/cord-270495-2u072mtp.txt summary: When SARS-CoV-2 is negative and clinical indication is present (at least fever and thrombocytopenia), DENV NS1 antigen and/or IgM/IgG antibody testing may be performed. Clinicians from Singapore reported two COVID-19 cases that were misdiagnosed as dengue among patients who presented with clinical manifestations and hematology profiles, suggesting dengue infection and false-positive DENV IgM antibody using a rapid diagnostic test (RDT). COVID-19 cases were defined as inpatients who met the COVID-19 criteria based on a predetermined combination of symptoms, laboratory testing, imaging, and risk exposure at Tangerang District Hospital, Indonesia (see Supplemental Table 1 ), and had a positive nasopharyngeal or oropharyngeal real-time RT-PCR for SARS-CoV-2. None of the 42 subjects was positive for dengue NS1 or showed seroconversion or increasing DENV IgM and IgG index values, suggesting no acute DENV infection among these COVID-19 cases. The third patient did not recall having a fever before acute COVID-19 illness, suggesting asymptomatic or mild dengue, the most common presentation of DENV infection. abstract: Emergence of SARS-CoV-2 in dengue virus (DENV)–endemic areas complicates the diagnosis of both infections. COVID-19 cases may be misdiagnosed as dengue, particularly when relying on DENV IgM, which can remain positive months after infection. To estimate the extent of this problem, we evaluated sera from 42 confirmed COVID-19 patients for evidence of DENV infection. No cases of SARS-CoV-2 and DENV coinfection were identified. However, recent DENV infection, indicated by the presence of DENV IgM and/or high level of IgG antibodies, was found in seven patients. Dengue virus IgM and/or high IgG titer should not exclude COVID-19. SARS-CoV-2 reverse transcription polymerase chain reaction (RT-PCR) testing is appropriate when dengue nonstructural protein 1 (NS1) or RT-PCR is negative. Given the possibility of coinfection, testing for both DENV and SARS-CoV-2 is merited in the setting of the current pandemic. url: https://www.ncbi.nlm.nih.gov/pubmed/32762798/ doi: 10.4269/ajtmh.20-0676 id: cord-002581-r7mskri0 author: Magnani, Diogo M. title: A human inferred germline antibody binds to an immunodominant epitope and neutralizes Zika virus date: 2017-06-12 words: nan sentences: nan pages: flesch: nan cache: txt: summary: abstract: The isolation of neutralizing monoclonal antibodies (nmAbs) against the Zika virus (ZIKV) might lead to novel preventative strategies for infections in at-risk individuals, primarily pregnant women. Here we describe the characterization of human mAbs from the plasmablasts of an acutely infected patient. One of the 18 mAbs had the unusual feature of binding to and neutralizing ZIKV despite not appearing to have been diversified by affinity maturation. This mAb neutralized ZIKV (Neut(50) ~ 2 μg/ml) but did not react with any of the four dengue virus serotypes. Except for the expected junctional diversity created by the joining of the V-(D)-J genes, there was no deviation from immunoglobulin germline genes. This is a rare example of a human mAb with neutralizing activity in the absence of detectable somatic hypermutation. Importantly, binding of this mAb to ZIKV was specifically inhibited by human plasma from ZIKV-exposed individuals, suggesting that it may be of value in a diagnostic setting. url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5481143/ doi: 10.1371/journal.pntd.0005655 id: cord-033331-giku34r9 author: Manrique-Saide, Pablo title: The TIRS trial: protocol for a cluster randomized controlled trial assessing the efficacy of preventive targeted indoor residual spraying to reduce Aedes-borne viral illnesses in Merida, Mexico date: 2020-10-08 words: nan sentences: nan pages: flesch: nan cache: txt: summary: abstract: BACKGROUND: Current urban vector control strategies have failed to contain dengue epidemics and to prevent the global expansion of Aedes-borne viruses (ABVs: dengue, chikungunya, Zika). Part of the challenge in sustaining effective ABV control emerges from the paucity of evidence regarding the epidemiological impact of any Aedes control method. A strategy for which there is limited epidemiological evidence is targeted indoor residual spraying (TIRS). TIRS is a modification of classic malaria indoor residual spraying that accounts for Aedes aegypti resting behavior by applying residual insecticides on exposed lower sections of walls (< 1.5 m), under furniture, and on dark surfaces. METHODS/DESIGN: We are pursuing a two-arm, parallel, unblinded, cluster randomized controlled trial to quantify the overall efficacy of TIRS in reducing the burden of laboratory-confirmed ABV clinical disease (primary endpoint). The trial will be conducted in the city of Merida, Yucatan State, Mexico (population ~ 1million), where we will prospectively follow 4600 children aged 2–15 years at enrollment, distributed in 50 clusters of 5 × 5 city blocks each. Clusters will be randomly allocated (n = 25 per arm) using covariate-constrained randomization. A “fried egg” design will be followed, in which all blocks of the 5 × 5 cluster receive the intervention, but all sampling to evaluate the epidemiological and entomological endpoints will occur in the “yolk,” the center 3 × 3 city blocks of each cluster. TIRS will be implemented as a preventive application (~ 1–2 months prior to the beginning of the ABV season). Active monitoring for symptomatic ABV illness will occur through weekly household visits and enhanced surveillance. Annual sero-surveys will be performed after each transmission season and entomological evaluations of Ae. aegypti indoor abundance and ABV infection rates monthly during the period of active surveillance. Epidemiological and entomological evaluation will continue for up to three transmission seasons. DISCUSSION: The findings from this study will provide robust epidemiological evidence of the efficacy of TIRS in reducing ABV illness and infection. If efficacious, TIRS could drive a paradigm shift in Aedes control by considering Ae. aegypti behavior to guide residual insecticide applications and changing deployment to preemptive control (rather than in response to symptomatic cases), two major enhancements to existing practice. TRIAL REGISTRATION: ClinicalTrials.gov NCT04343521. Registered on 13 April 2020. The protocol also complies with the WHO International Clinical Trials Registry Platform (ICTRP) (Additional file 1). PRIMARY SPONSOR: National Institutes of Health, National Institute of Allergy and Infectious Diseases (NIH/NIAID). url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7542575/ doi: 10.1186/s13063-020-04780-7 id: cord-355489-tkvfneje author: Mendez, Jairo A title: Phylogenetic history demonstrates two different lineages of dengue type 1 virus in Colombia date: 2010-09-14 words: 4615.0 sentences: 235.0 pages: flesch: 48.0 cache: ./cache/cord-355489-tkvfneje.txt txt: ./txt/cord-355489-tkvfneje.txt summary: Yet, the phylogenetic relationships between strains isolated along the covered period of time suggests that viral strains detected in some years, although belonging to the same genotype V, have different recent origins corresponding to multiple re-introduction events of viral strains that were circulating in neighbor countries. Due to the importance of DENV in public health, the particular goals of this research were to reconstruct the phylogenetic history of DENV-1 and to date the phylogenetic tree using isolation time as calibration points to establish date of introduction of virus and rate evolution patterns of virus in Colombia. Previously reported genotypes were represented in the tree and placed most of the Colombian isolates nesting in the genotype V clade (America, Africa) and were closely related to Argentina, Brazil and Paraguay virus strains. abstract: BACKGROUND: Dengue Fever is one of the most important viral re-emergent diseases affecting about 50 million people around the world especially in tropical and sub-tropical countries. In Colombia, the virus was first detected in the earliest 70's when the disease became a major public health concern. Since then, all four serotypes of the virus have been reported. Although most of the huge outbreaks reported in this country have involved dengue virus serotype 1 (DENV-1), there are not studies about its origin, genetic diversity and distribution. RESULTS: We used 224 bp corresponding to the carboxyl terminus of envelope (E) gene from 74 Colombian isolates in order to reconstruct phylogenetic relationships and to estimate time divergences. Analyzed DENV-1 Colombian isolates belonged to the formerly defined genotype V. Only one virus isolate was clasified in the genotype I, likely representing a sole introduction that did not spread. The oldest strains were closely related to those detected for the first time in America in 1977 from the Caribbean and were detected for two years until their disappearance about six years later. Around 1987, a split up generated 2 lineages that have been evolving separately, although not major aminoacid changes in the analyzed region were found. CONCLUSION: DENV-1 has been circulating since 1978 in Colombia. Yet, the phylogenetic relationships between strains isolated along the covered period of time suggests that viral strains detected in some years, although belonging to the same genotype V, have different recent origins corresponding to multiple re-introduction events of viral strains that were circulating in neighbor countries. Viral strains used in the present study did not form a monophyletic group, which is evidence of a polyphyletic origin. We report the rapid spread patterns and high evolution rate of the different DENV-1 lineages. url: https://doi.org/10.1186/1743-422x-7-226 doi: 10.1186/1743-422x-7-226 id: cord-355906-yeaw9nr8 author: Nedjadi, Taoufik title: Tackling dengue fever: Current status and challenges date: 2015-12-09 words: nan sentences: nan pages: flesch: nan cache: txt: summary: abstract: According to recent statistics, 96 million apparent dengue infections were estimated worldwide in 2010. This figure is by far greater than the WHO prediction which indicates the rapid spread of this disease posing a growing threat to the economy and a major challenge to clinicians and health care services across the globe particularly in the affected areas. This article aims at bringing to light the current epidemiological and clinical status of the dengue fever. The relationship between genetic mutations, single nucleotide polymorphism (SNP) and the pathophysiology of disease progression will be put into perspective. It will also highlight the recent advances in dengue vaccine development. Thus far, a significant progress has been made in unraveling the risk factors and understanding the molecular pathogenesis associated with the disease. However, further insights in molecular features of the disease and the development of animal models will enormously help improving the therapeutic interventions and potentially contribute to finding new preventive measures for population at risk. url: https://www.ncbi.nlm.nih.gov/pubmed/26645066/ doi: 10.1186/s12985-015-0444-8 id: cord-276718-3lujp0oy author: Neeraja, M. title: Rapid detection and differentiation of dengue virus serotypes by NS1 specific reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay in patients presenting to a tertiary care hospital in Hyderabad, India date: 2014-10-24 words: 6142.0 sentences: 296.0 pages: flesch: 54.0 cache: ./cache/cord-276718-3lujp0oy.txt txt: ./txt/cord-276718-3lujp0oy.txt summary: title: Rapid detection and differentiation of dengue virus serotypes by NS1 specific reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay in patients presenting to a tertiary care hospital in Hyderabad, India In the present study, the standardization and validation of a one step, four tube reverse transcription loop-mediated isothermal amplification assay (RT-LAMP) for rapid detection and serotyping of the DENV targeting NS1 gene using the Genie® II flourometer was carried out. In the present study, the RT-LAMP assay was developed for the detection and serotyping of DENV infection targeting the serotype specific regions of the NS1 gene using a real-time flourometer (Genie ® II from Optigene, U.K.). The performance of the RT-LAMP assay was validated by testing the samples simultaneously by the CDC real time PCR that is most sensitive and specific method for detection and differentiation of the DENV (CDC Dengue). Rapid detection and differentiation of dengue virus serotypes by a real-time reverse transcription-loop-mediated isothermal amplification assay abstract: Early and rapid detection of dengue virus (DENV) infection during the acute phase of illness is crucial for proper patient management and prevention of the spread of the infection. In the present study, the standardization and validation of a one step, four tube reverse transcription loop-mediated isothermal amplification assay (RT-LAMP) for rapid detection and serotyping of the DENV targeting NS1 gene using the Genie® II flourometer was carried out. The performance of the RT-LAMP was compared to RT-PCR, CDC 1-4 Real time PCR and the NS1 antigen ELISA, IgM and IgG anti DENV antibodies. Acute DENV infection was confirmed in 250/300 patients suspected clinically of DENV infection. RT- LAMP and CDC 1-4 Real time PCR assay was positive in 148/250 patients, while 92/250 patients were positive for anti- Dengue IgM and IgG antibodies. The RT-LAMP assay and the CDC real-time RT-PCR assay showed high concordance (k = 1.0). The detection rate of acute DENV infection improved to 96% (240/250) when the results of RT-LAMP were combined with NS1 Ag, IgM and IgG ELISA. The RT-LAMP had a detection limit of 100 copies for DEN-1 and DEN-2, 10 copies for DEN-3 and DEN-4 compared to 1000 copies for DEN-1 and DEN-2, 100 copies for DEN-3 and DEN-4 by the conventional RT-PCR. The assay showed 100% specificity. The RT-LAMP assay developed in this study has potential use for early clinical diagnosis, serotyping and surveillance of DENV infection in endemic countries such as India. url: https://www.ncbi.nlm.nih.gov/pubmed/25455901/ doi: 10.1016/j.jviromet.2014.10.005 id: cord-326840-piv6h7oq author: Paemanee, Atchara title: Screening of melatonin, α-tocopherol, folic acid, acetyl-l-carnitine and resveratrol for anti-dengue 2 virus activity date: 2018-05-16 words: nan sentences: nan pages: flesch: nan cache: txt: summary: abstract: OBJECTIVE: Infections with the mosquito transmitted dengue virus (DENV) are a significant public health burden in many parts of the world. Despite the introduction of a commercial vaccine in some parts of the world, the majority of the populations at risk of infection remain unprotected against this disease, and there is currently no treatment for DENV infection. Natural compounds offer the prospect of cheap and sustainable therapeutics to reduce the disease burden during infection, and thus potentially alleviate the risk of more severe disease. This study evaluated the potential anti-DENV 2 activity of five natural compounds namely melatonin, α-tocopherol, folic acid, acetyl-l-carnitine and resveratrol in two different cell lines. RESULTS: Screening of the compounds showed that one compound (acetyl-l-carnitine) showed no effect on DENV infection, three compounds (melatonin, α-tocopherol and folic acid) slightly increased levels of infection, while the 5th compound, resveratrol, showed some limited anti-DENV activity, with resveratrol reducing virus output with an EC(50) of less than 25 μM. These results suggest that some commonly taken natural compounds may have beneficial effects on DENV infection, but that others may potentially add to the disease burden. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13104-018-3417-3) contains supplementary material, which is available to authorized users. url: https://www.ncbi.nlm.nih.gov/pubmed/29769094/ doi: 10.1186/s13104-018-3417-3 id: cord-306204-rkher4ly author: Pong, Lian Yih title: Anti-dengue virus serotype 2 activity of tannins from porcupine dates date: 2020-05-20 words: 6060.0 sentences: 318.0 pages: flesch: 54.0 cache: ./cache/cord-306204-rkher4ly.txt txt: ./txt/cord-306204-rkher4ly.txt summary: Vero cells grown in 24-well plates were infected with 30-50 FFU of DENV-2 in the presence or absence of BDTF, PDTF, methanol crude extracts, tannic acids of FT and ST at maximum non-toxic concentration (MNTC) of 100 µg/ mL, 50 µg/mL, 200 µg/mL, 50 µg/mL and 25 µg/mL respectively for 1 h at 4 °C. To study whether tannins in the porcupine dates are bioactive against dengue infection, we have isolated the tannin fractions from MBD and MPD for Besides that, both BDTF and PDTF demonstrated similar and higher virucidal activity against DENV-2 when compared to their methanol crude extracts. " indicates not significant Fig. 9 Effect of pre-treatment with porcupine dates extracts and tannic acids on DENV-2 infection in Vero cells. The antiviral activity ascribed to the interaction between the extracts and host cells was also investigated; pre-treating the cells with BDTF prevented the DENV-2 infection by at least 75% and similar result was observed for FT tannic acid. abstract: BACKGROUND: Dengue fever is currently endemic in tropical and subtropical countries worldwide and effective drug against DENV infection is still unavailable. Porcupine dates, which are traditionally used to treat dengue fever, might contain potential anti-dengue compounds. Two porcupine dates, black date (BD) and powdery date (PD) from Himalayan porcupine (Hystrix brachyura), were investigated for their antiviral activities against DENV-2 in vitro. METHODS: The methanol crude extracts (MBD and MPD) were prepared from the raw material of porcupine dates. The tannin-rich fractions (BDTF and PDTF) were isolated from their methanol crude extracts using column chromatography. The presence of tannins in BDTF and PDTF extracts was determined by fourier-transform infrared spectroscopy (FTIR) and nuclear magnetic resonance (NMR) analyses. The cytotoxicity and anti-DENV-2 activities including virus yield inhibition, virucidal, virus attachment and pre-treatment assays of the extracts were examined in Vero cells. RESULTS: Our findings revealed that all the extracts of porcupine dates exhibited antiviral activity against DENV-2 in Vero cells. The IC(50) of BDTF and PDTF were 25 µg/mL and 11 µg/mL respectively, while their methanol crude extracts demonstrated lower antiviral efficacy (IC(50) ≈ 101–107 µg/mL). BDTF and PDTF also exerted a similar higher virucidal effect (IC(50) of 11 µg/mL) than methanol crude extracts (IC(50) ≈ 52–66 µg/mL). Furthermore, all the extracts inhibited the attachment of DENV-2 by at least 80%. Pre-treatments of cells with BDTF and PDTF markedly prevented DENV-2 infection when compared to methanol crude extracts. CONCLUSION: This study suggests that porcupine dates possess antiviral properties against DENV-2, which is attributed to its tannin compounds. url: https://www.ncbi.nlm.nih.gov/pubmed/32467721/ doi: 10.1186/s13020-020-00329-7 id: cord-253480-qchrw337 author: Pu, Jieying title: Antiviral activity of Carbenoxolone disodium against dengue virus infection date: 2016-12-23 words: 5289.0 sentences: 287.0 pages: flesch: 52.0 cache: ./cache/cord-253480-qchrw337.txt txt: ./txt/cord-253480-qchrw337.txt summary: Here, we found that the production of infectious DENV particles was significantly decreased by CBX treatment in DENV‐permissive cells, while the viral RNA and viral protein synthesis were not affected. Moreover, results from time-of-addition study showed that the inhibitory effect of CBX on DENV was exhibited by targeting the virus itself, not the host cells. In this study, we investigated whether CBX treatment inhibits dengue infection by measuring the production of progeny virions and viral RNA as well as viral protein expression. To determine whether CBX inhibits the production of infectious progeny DENV in other dengue-permissive cells, TCID 50 assay was performed to measure the virus titer in CBX-treated THP-1 and HUVEC cells, both of which are widely used in DENV studies [Halstead, 1988; Wu et al., 2000] . CBX treatment did not inhibit DENV-1 or DENV-2 RNA synthesis and protein expression in one replication cycle, but markedly reduced progeny virus The total RNA was isolated from the infected cells and analyzed by quantitative RT-PCR. abstract: As one of the most important mosquito‐borne viral diseases, dengue infection is now becoming a global concern due to its rapid spread and rise in incidence. Currently, there is no approved vaccine or effective antiviral drug for dengue virus (DENV) infection. Glycyrrhetinic acid (GNa) and its related derivatives have been reported to inhibit a broad spectrum of viruses. However, it is unknown whether Carbenoxolone disodium (CBX), one of the GNa derivatives, affects DENV infection. Here, we found that the production of infectious DENV particles was significantly decreased by CBX treatment in DENV‐permissive cells, while the viral RNA and viral protein synthesis were not affected. Moreover, results from time‐of‐addition study showed that the inhibitory effect of CBX on DENV was exhibited by targeting the virus itself, not the host cells. Directly incubating DENV with CBX resulted in a remarkable reduction of virus titer and virus infectivity. Furthermore, DENV RNA from progeny virions in the supernatants was significantly decreased by CBX treatment in a dose‐dependent manner. Taken together, these data indicate that the antiviral activity of CBX against DENV may be mainly due to a virucidal effect exerted by the compound itself. Our work, for the first time, demonstrates that CBX has antiviral activity against DENV infection, providing useful information for development of potential therapeutic interventions against dengue. J. Med. Virol. 89:571–581, 2017. © 2016 Wiley Periodicals, Inc. url: https://www.ncbi.nlm.nih.gov/pubmed/27155198/ doi: 10.1002/jmv.24571 id: cord-011012-5mev3otu author: Rathore, Abhishek Singh title: Production and immunogenicity of Fubc subunit protein redesigned from DENV envelope protein date: 2020-03-30 words: nan sentences: nan pages: flesch: nan cache: txt: summary: abstract: Dengue virus (DENV) is a vector-borne human pathogen that usually causes dengue fever; however, sometime it leads to deadly complications such as dengue with warning signs (DWS+) and severe dengue (SD). Several studies have shown that fusion (Fu) and bc loop of DENV envelope domain II are highly conserved and consist some of the most dominant antigenic epitopes. Therefore, in this study, Fu and bc loops were joined together to develop a short recombinant protein as an alternative of whole DENV envelope protein, and its immunogenic potential as fusion peptide was estimated. For de novo designing of the antigen, Fu and bc peptides were linked with an optimised linker so that the three dimensional conformation was maintained as it is in DENV envelope protein. The redesigned Fubc protein was expressed in E. coli and purified. Subsequently, structural integrity of the purified protein was verified by CD spectroscopy. To characterise immune responses against recombinant Fubc protein, BALB/c mice were subcutaneously injected with emulsified antigen preparation. It was observed by ELISA that Fubc fusion protein elicited higher serum IgG antibody response either in the presence or in absence of Freund’s adjuvant in comparison to the immune response of Fu and bc peptides separately. Furthermore, the binding of Fubc protein with mice antisera was validated by SPR analysis. These results suggest that Fu and bc epitope-based recombinant fusion protein could be a potential candidate towards the development of the effective subunit vaccine against DENV. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00253-020-10541-y) contains supplementary material, which is available to authorized users. url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7223326/ doi: 10.1007/s00253-020-10541-y id: cord-345898-a6vt8kso author: Ren, Linzhu title: Live Cell Reporter Systems for Positive-Sense Single Strand RNA Viruses date: 2016-01-04 words: 6000.0 sentences: 289.0 pages: flesch: 38.0 cache: ./cache/cord-345898-a6vt8kso.txt txt: ./txt/cord-345898-a6vt8kso.txt summary: There are three types of cell-based reporter systems that express certain reporter protein for positive-sense single strand RNA virus infections. An RVP is a type of virus-like particle (VLP) composed of viral structural proteins and a self-replicating replicon RNA containing a reporter gene [17, 63] . However, when the cells were infected with the specific virus, the viral RdRp was provided by the virus and the defective replicon was activated, resulting in high-level expression of the reporter gene, which could be easily examined [44] . However, when the cells were infected with the specific virus, the viral RdRp was provided by the virus and the defective replicon was activated, resulting in high-level expression of the reporter gene, which could be easily examined [44] . Development of Dengue type-2 virus replicons expressing GFP reporter gene in study of viral RNA replication abstract: Cell-based reporter systems have facilitated studies of viral replication and pathogenesis, virus detection, and drug susceptibility testing. There are three types of cell-based reporter systems that express certain reporter protein for positive-sense single strand RNA virus infections. The first type is classical reporter system, which relies on recombinant virus, reporter virus particle, or subgenomic replicon. During infection with the recombinant virus or reporter virus particle, the reporter protein is expressed and can be detected in real time in a dose-dependent manner. Using subgenomic replicon, which are genetically engineered viral RNA molecules that are capable of replication but incapable of producing virions, the translation and replication of the replicon could be tracked by the accumulation of reporter protein. The second type of reporter system involves genetically engineered cells bearing virus-specific protease cleavage sequences, which can sense the incoming viral protease. The third type is based on viral replicase, which can report the specific virus infection via detection of the incoming viral replicase. This review specifically focuses on the major technical breakthroughs in the design of cell-based reporter systems and the application of these systems to the further understanding and control of viruses over the past few decades. url: https://doi.org/10.1007/s12010-015-1968-5 doi: 10.1007/s12010-015-1968-5 id: cord-294842-aesiff1f author: Romero-Brey, Inés title: Membranous Replication Factories Induced by Plus-Strand RNA Viruses date: 2014-07-22 words: nan sentences: nan pages: flesch: nan cache: txt: summary: abstract: In this review, we summarize the current knowledge about the membranous replication factories of members of plus-strand (+) RNA viruses. We discuss primarily the architecture of these complex membrane rearrangements, because this topic emerged in the last few years as electron tomography has become more widely available. A general denominator is that two “morphotypes” of membrane alterations can be found that are exemplified by flaviviruses and hepaciviruses: membrane invaginations towards the lumen of the endoplasmatic reticulum (ER) and double membrane vesicles, representing extrusions also originating from the ER, respectively. We hypothesize that either morphotype might reflect common pathways and principles that are used by these viruses to form their membranous replication compartments. url: https://doi.org/10.3390/v6072826 doi: 10.3390/v6072826 id: cord-256732-md1u51va author: Shrivastava, Gaurav title: Inflammasome Fuels Dengue Severity date: 2020-09-10 words: 8878.0 sentences: 474.0 pages: flesch: 41.0 cache: ./cache/cord-256732-md1u51va.txt txt: ./txt/cord-256732-md1u51va.txt summary: Inflammasome plays a vital function in the host innate immune system by regulating the secretion of proinflammatory cytokines (IL-1β and IL-18) and subsequent induction of "pyroptosis, " a form of programmed cell death, activated by inflammatory caspases (Fink and Cookson, 2006; Lamkanfi and Dixit, 2014) . IL-1β secretion further enhances the production and release of IL-23 and IL-6 and the association of IL-18, IL-1β, and IL-23 stimulate Th17/γδ T cells to generate pro-inflammatory cytokines (GM-CSF, IL-17A, IL-17F, IL-22,) that create the stage for host adaptive immune responses during DENV infection (Wu et al., 2013a) . Another study demonstrated that DENV infects DC and induces NLRP3 inflammasome activation, triggering ROS production in mitochondria that leads to the discharge of mitochondrial DNA (mtDNA) into the cytosol. (D) DENV activates platelets and induces the secretion of EVs (DV-EXOs and DV-MVs), that results in the CLEC5A and TLR2 mediated release of proinflammatory cytokines and NETs formation in neutrophils contributing to vascular leakage during dengue infection. abstract: Dengue is an acute febrile disease triggered by dengue virus. Dengue is the widespread and rapidly transmitted mosquito-borne viral disease of humans. Diverse symptoms and diseases due to Dengue virus (DENV) infection ranges from dengue fever, dengue hemorrhagic fever (life-threatening) and dengue shock syndrome characterized by shock, endothelial dysfunction and vascular leakage. Several studies have linked the severity of dengue with the induction of inflammasome. DENV activates the NLRP3-specific inflammasome in DENV infected human patients, mice; specifically, mouse bone marrow derived macrophages (BMDMs), dendritic cells, endothelial cells, human peripheral blood mononuclear cells (PBMCs), keratinocytes, monocyte-differentiated macrophages (THP-1), and platelets. Dengue virus mediated inflammasome initiates the maturation of IL-1β and IL-18, which are critical for dengue pathology and inflammatory response. Several studies have reported the molecular mechanism through which (host and viral factors) dengue induces inflammasome, unravels the possible mechanisms of DENV pathogenesis and sets up the stage for the advancement of DENV therapeutics. In this perspective article, we discuss the potential implications and our understanding of inflammasome mechanisms of dengue virus and highlight research areas that have potential to inhibit the pathogenesis of viral diseases, specifically for dengue. url: https://www.ncbi.nlm.nih.gov/pubmed/33014899/ doi: 10.3389/fcimb.2020.00489 id: cord-282204-j1slaefb author: Silva, José V.J. title: A scoping review of Chikungunya virus infection: epidemiology, clinical characteristics, viral co-circulation complications, and control date: 2018-12-31 words: 8010.0 sentences: 464.0 pages: flesch: 43.0 cache: ./cache/cord-282204-j1slaefb.txt txt: ./txt/cord-282204-j1slaefb.txt summary: de; Oliveira, Renato A.S.; Durães-Carvalho, Ricardo; Lopes, Thaísa R.R.; Silva, Daisy E.A.; Gil, Laura H.V.G. title: A scoping review of Chikungunya virus infection: epidemiology, clinical characteristics, viral co-circulation complications, and control Laboratory tests for specific diagnosis of CHIKV infection are based on virus isolation, viral RNA detection and serology (Johnson et al., 2016) . Anti-CHIKV candidates that have been already tested in humans and/or animals include inactivated-, attenuated-, virus like particle-(VLP), DNA-and chimeric vaccines (Eckels et al., 1970; Levitt et al., 1986; Muthumani et al., 2008; Wang et al., 2008; Tiwari et al., 2009; Sharma et al., 2012 Akahata et al., 2010 Plante et al., 2011; Wang et al., 2011; Gorchakov et al., 2012; Brandler et al., 2013; Chang et al., 2014; García-Arriaza et al., 2014; Tretyakova et al., 2014; van den Doel et al., 2014; Erasmus et al., 2017) . abstract: Abstract Chikungunya fever is a mosquito-borne viral illness characterized by a sudden onset of fever associated with joint pains. It was first described in the 1950s during a Chikungunya virus (CHIKV) outbreak in southern Tanzania and has since (re-) emerged and spread to several other geographical areas, reaching large populations and causing massive epidemics. In recent years, CHIKV has gained considerable attention due to its quick spread to the Caribbean and then in the Americas, with many cases reported between 2014 and 2017. CHIKV has further garnered attention due to the clinical diagnostic difficulties when Zika (ZIKV) and dengue (DENV) viruses are simultaneously present. In this review, topical CHIKV-related issues, such as epidemiology and transmission, are examined. The different manifestations of infection (acute, chronic and atypical) are described and a particular focus is placed upon the diagnostic handling in the case of ZIKV and DENV co-circulating. Natural and synthetic compounds under evaluation for treatment of chikungunya disease, including drugs already licensed for other purposes, are also discussed. Finally, previous and current vaccine strategies, as well as the control of the CHIKV transmission through an integrated vector management, are reviewed in some detail. url: https://doi.org/10.1016/j.actatropica.2018.09.003 doi: 10.1016/j.actatropica.2018.09.003 id: cord-319174-pbqjg7hf author: Song, Ke-Yu title: A novel reporter system for neutralizing and enhancing antibody assay against dengue virus date: 2014-02-18 words: nan sentences: nan pages: flesch: nan cache: txt: summary: abstract: BACKGROUND: Dengue virus (DENV) still poses a global public health threat, and no vaccine or antiviral therapy is currently available. Antibody plays distinct roles in controlling DENV infections. Neutralizing antibody is protective against DENV infection, whereas sub-neutralizing concentration of antibody can increase DENV infection, termed antibody-dependent enhancement (ADE). Plaque-based assay represents the most widely accepted method measuring neutralizing or enhancing antibodies. RESULTS: In this study, a novel reporter virus-based system was developed for measuring neutralization and ADE activity. A stable Renilla luciferase reporter DENV (Luc-DENV) that can produce robust luciferase signals in BHK-21 and K562 cells were used to establish the assay and validated against traditional plaque-based assay. Luciferase value analysis using various known DENV-specific monoclonal antibodies showed good repeatability and a well linear correlation with conventional plaque-based assays. The newly developed assay was finally validated with clinical samples from infected animals and individuals. CONCLUSIONS: This reporter virus-based assay for neutralizing and enhancing antibody evaluation is rapid, lower cost, and high throughput, and will be helpful for laboratory detection and epidemiological investigation for DENV antibodies. url: https://doi.org/10.1186/1471-2180-14-44 doi: 10.1186/1471-2180-14-44 id: cord-286255-ded5t1ai author: Tomashek, Kay M. title: Clinical and epidemiologic characteristics of dengue and other etiologic agents among patients with acute febrile illness, Puerto Rico, 2012–2015 date: 2017-09-13 words: 6132.0 sentences: 315.0 pages: flesch: 53.0 cache: ./cache/cord-286255-ded5t1ai.txt txt: ./txt/cord-286255-ded5t1ai.txt summary: Blood and oro-nasopharyngeal specimens were tested by RT-PCR and immunodiagnostic methods for infection with dengue viruses (DENV) 1–4, chikungunya virus (CHIKV), influenza A and B viruses (FLU A/B), 12 other respiratory viruses (ORV), enterovirus, Leptospira spp., and Burkholderia pseudomallei. Clinical predictors of laboratory-positive dengue compared to all other AFI etiologies were determined by age and day post-illness onset (DPO) at presentation. By enrolling febrile patients at clinical presentation, we identified unbiased predictors of laboratory-positive dengue as compared to other common causes of AFI. Among 6,349 participants who presented early (<3 DPO) in the clinical course, leukopenia, thrombocytopenia, headache, eye pain, nausea, and dizziness were significant positive predictors of laboratory-positive dengue as compared to all other AFI cases across all age groups ( Table 5 ). Of note, 6% of participants !65 years old had dengue as a cause of AFI, a finding comparable to a Puerto Rico study in which 5% of 17,666 laboratory-positive dengue cases detected by surveillance were !65 years old [36] . abstract: Identifying etiologies of acute febrile illnesses (AFI) is challenging due to non-specific presentation and limited availability of diagnostics. Prospective AFI studies provide a methodology to describe the syndrome by age and etiology, findings that can be used to develop case definitions and multiplexed diagnostics to optimize management. We conducted a 3-year prospective AFI study in Puerto Rico. Patients with fever ≤7 days were offered enrollment, and clinical data and specimens were collected at enrollment and upon discharge or follow-up. Blood and oro-nasopharyngeal specimens were tested by RT-PCR and immunodiagnostic methods for infection with dengue viruses (DENV) 1–4, chikungunya virus (CHIKV), influenza A and B viruses (FLU A/B), 12 other respiratory viruses (ORV), enterovirus, Leptospira spp., and Burkholderia pseudomallei. Clinical presentation and laboratory findings of participants infected with DENV were compared to those infected with CHIKV, FLU A/B, and ORV. Clinical predictors of laboratory-positive dengue compared to all other AFI etiologies were determined by age and day post-illness onset (DPO) at presentation. Of 8,996 participants enrolled from May 7, 2012 through May 6, 2015, more than half (54.8%, 4,930) had a pathogen detected. Pathogens most frequently detected were CHIKV (1,635, 18.2%), FLU A/B (1,074, 11.9%), DENV 1–4 (970, 10.8%), and ORV (904, 10.3%). Participants with DENV infection presented later and a higher proportion were hospitalized than those with other diagnoses (46.7% versus 27.3% with ORV, 18.8% with FLU A/B, and 11.2% with CHIKV). Predictors of dengue in participants presenting <3 DPO included leukopenia, thrombocytopenia, headache, eye pain, nausea, and dizziness, while negative predictors were irritability and rhinorrhea. Predictors of dengue in participants presenting 3–5 DPO were leukopenia, thrombocytopenia, facial/neck erythema, nausea, eye pain, signs of poor circulation, and diarrhea; presence of rhinorrhea, cough, and red conjunctiva predicted non-dengue AFI. By enrolling febrile patients at clinical presentation, we identified unbiased predictors of laboratory-positive dengue as compared to other common causes of AFI. These findings can be used to assist in early identification of dengue patients, as well as direct anticipatory guidance and timely initiation of correct clinical management. url: https://www.ncbi.nlm.nih.gov/pubmed/28902845/ doi: 10.1371/journal.pntd.0005859 id: cord-318614-518giv0m author: Tsai, Jih-Jin title: A fully automated sample-to-answer PCR system for easy and sensitive detection of dengue virus in human serum and mosquitos date: 2019-07-10 words: 4825.0 sentences: 213.0 pages: flesch: 49.0 cache: ./cache/cord-318614-518giv0m.txt txt: ./txt/cord-318614-518giv0m.txt summary: A pan-dengue virus (DENV) RT-iiPCR, targeting the 5'' untranslated region, was validated previously on the semi-automated POCKIT combo system (involving separate devices for nucleic acid extraction and PCR amplification/detection) to offer performance comparable to a laboratory real-time PCR. CONCLUSIONS/SIGNIFICANCE: With performance comparable to a previously validated system, the fully-automated PCR system allows applications of the pan-DENV reagent as a useful tool near points of need to facilitate easy, fast and effective detection of dengue virus and help mitigate versatile public health challenges in the control and management of dengue disease. Testing with the pan-DENV RT-iiPCR, the analytical and clinical performance of the fully automatic POCKIT Central system was comparable to those of the semi-automatic POCKI combo system, which was validated previously to offer performances equivalent to the CDC DENV1-4 real-time RT-PCR for the detection of DENV in human serum [7, 24, 26] . abstract: BACKGROUND: The insulated isothermal PCR (iiPCR) technology enables consistent PCR amplification and detection in a simple heating device. A pan-dengue virus (DENV) RT-iiPCR, targeting the 5’ untranslated region, was validated previously on the semi-automated POCKIT combo system (involving separate devices for nucleic acid extraction and PCR amplification/detection) to offer performance comparable to a laboratory real-time PCR. Working on the same technologies, a compact automated sample-in-answer-out system (POCKIT Central Nucleic Acid Analyser) has been available commercially for iiPCR, minimizing human error risks and allowing easy molecular bio-detection near points of need. Here, we evaluated the analytical and clinical performance of the pan-DENV RT-iiPCR on the fully automated system by comparison to those on the semi-automated system. METHODOLOGY/PRINCIPAL FINDINGS: Testing sera containing serial diluted DENV-1, -2, -3, or -4 cell culture stock, the pan-DENV RT-iiPCR system had similar 100% detection endpoints on the two systems; i.e. at 1, 10, 1 and 10 PFU/ml, respectively, on the fully automated system, and at 10, 1, 10 and 10 PFU/ml, respectively, on the semi-automated system. Furthermore, both fully automated and semi-automated PCR system can detect all four DENV serotypes in mosquitos. Clinical performance of the reagent on the two systems was evaluated by testing 60 human serum samples. Both systems detected the same 40 samples (ten DENV-1, -2, -3, and -4 positive each) and did not detect the other 20; 100% agreement (κ = 1) was found between the two systems. CONCLUSIONS/SIGNIFICANCE: With performance comparable to a previously validated system, the fully-automated PCR system allows applications of the pan-DENV reagent as a useful tool near points of need to facilitate easy, fast and effective detection of dengue virus and help mitigate versatile public health challenges in the control and management of dengue disease. url: https://doi.org/10.1371/journal.pone.0218139 doi: 10.1371/journal.pone.0218139 id: cord-344020-8poerd09 author: Vermeulen, Tom D title: Autochthonous dengue in two Dutch tourists visiting Département Var, southern France, July 2020 date: 2020-10-01 words: 1827.0 sentences: 110.0 pages: flesch: 54.0 cache: ./cache/cord-344020-8poerd09.txt txt: ./txt/cord-344020-8poerd09.txt summary: We report dengue virus (DENV) infection in two Dutch tourists who visited Département Var, southern France, in July and August 2020. We report dengue virus (DENV) infection in two Dutch tourists who visited Département Var, southern France, in July and August 2020. As some autochthonous dengue cases have occurred in Europe in recent years, awareness among physicians and public health experts about possible intermittent presence of DENV in southern Europe is important to minimise delay in diagnosis and treatment. As some autochthonous dengue cases have occurred in Europe in recent years, awareness among physicians and public health experts about possible intermittent presence of DENV in southern Europe is important to minimise delay in diagnosis and treatment. On August 27, upon confirmation of the serological results, Patient 1 was reported by the RIVM to the French authorities through the Early Warning and Response System of the European Union as an autochthonous DENV infection probably acquired in France with cross-border implication. abstract: We report dengue virus (DENV) infection in two Dutch tourists who visited Département Var, southern France, in July and August 2020. As some autochthonous dengue cases have occurred in Europe in recent years, awareness among physicians and public health experts about possible intermittent presence of DENV in southern Europe is important to minimise delay in diagnosis and treatment. Quick diagnosis can lead to timely action to contain the spread of vector-borne diseases and minimise transmission. url: https://www.ncbi.nlm.nih.gov/pubmed/33006305/ doi: 10.2807/1560-7917.es.2020.25.39.2001670 id: cord-002179-v8lpw4r7 author: Viktorovskaya, Olga V. title: Identification of RNA Binding Proteins Associated with Dengue Virus RNA in Infected Cells Reveals Temporally Distinct Host Factor Requirements date: 2016-08-24 words: 8021.0 sentences: 418.0 pages: flesch: 49.0 cache: ./cache/cord-002179-v8lpw4r7.txt txt: ./txt/cord-002179-v8lpw4r7.txt summary: title: Identification of RNA Binding Proteins Associated with Dengue Virus RNA in Infected Cells Reveals Temporally Distinct Host Factor Requirements Previously, we have described a high-throughput mass spectrometry method termed TUX-MS (thiouracil cross-linking mass spectrometry) to identify host factors that interact with viral RNA during a live infection in cell culture [15] . Development of a quantitative thiouridine cross-linking mass spectrometry (qTUX-MS) method for identification of proteins associated with the DENV RNA TUX-MS can be used to identify host factors by incorporating 4-thiouridine (4sU), a zero-distance cross-linker, into the viral RNA (vRNA) to enable cross-linking of proteins bound to vRNA during a live infection in cell culture [15] . Since some of hnRNPs are known to modulate cellular gene expression in response to dengue infection [60, 61] , we can not rule out that some of the observed effects on virus titers derive from their roles in regulating host mRNAs. Among the numerous qTUX-MS identified factors of interest, our study is the first to demonstrate the involvement of HMCES (or C3orf37) in viral infection. abstract: BACKGROUND: There are currently no vaccines or antivirals available for dengue virus infection, which can cause dengue hemorrhagic fever and death. A better understanding of the host pathogen interaction is required to develop effective therapies to treat DENV. In particular, very little is known about how cellular RNA binding proteins interact with viral RNAs. RNAs within cells are not naked; rather they are coated with proteins that affect localization, stability, translation and (for viruses) replication. METHODOLOGY/PRINCIPAL FINDINGS: Seventy-nine novel RNA binding proteins for dengue virus (DENV) were identified by cross-linking proteins to dengue viral RNA during a live infection in human cells. These cellular proteins were specific and distinct from those previously identified for poliovirus, suggesting a specialized role for these factors in DENV amplification. Knockdown of these proteins demonstrated their function as viral host factors, with evidence for some factors acting early, while others late in infection. Their requirement by DENV for efficient amplification is likely specific, since protein knockdown did not impair the cell fitness for viral amplification of an unrelated virus. The protein abundances of these host factors were not significantly altered during DENV infection, suggesting their interaction with DENV RNA was due to specific recruitment mechanisms. However, at the global proteome level, DENV altered the abundances of proteins in particular classes, including transporter proteins, which were down regulated, and proteins in the ubiquitin proteasome pathway, which were up regulated. CONCLUSIONS/SIGNIFICANCE: The method for identification of host factors described here is robust and broadly applicable to all RNA viruses, providing an avenue to determine the conserved or distinct mechanisms through which diverse viruses manage the viral RNA within cells. This study significantly increases the number of cellular factors known to interact with DENV and reveals how DENV modulates and usurps cellular proteins for efficient amplification. url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4996428/ doi: 10.1371/journal.pntd.0004921 id: cord-276364-zyw5aukk author: Wong, Ho Him title: Manipulation of autophagy by (+) RNA viruses date: 2019-08-08 words: 6884.0 sentences: 360.0 pages: flesch: 33.0 cache: ./cache/cord-276364-zyw5aukk.txt txt: ./txt/cord-276364-zyw5aukk.txt summary: Over the past few decades, a growing body of research has defined the critical role of this pathway in facilitating infection by numerous +RNA RNA viruses, including poliovirus (PV) [7, 8] , Coxsackievirus B3 (CVB3) [9, 10] , CVB4 [11] , Enterovirus 71 (EV71) [12] , Human rhinovirus (HRV) [13] , Foot-and-mouth disease virus (FMDV) [14] , encephalomyocarditis virus (EMCV) [15] , Dengue virus (DENV) [16, 17] , Zika virus (ZIKV) [18, 19] , Hepatitis C virus (HCV) [20] , Mouse hepatitic virus (MHV), Newcastle disease virus (NDV) [21] , Severe and acute respiratory syndrome coronavirus (SARS-CoV) [22] , Chikungunya virus (ChikV) [23] , and Japanese encephalitis virus (JEV) [24] . Delineating the process of viral assembly from replication is technically challenging, especially since both processes would very likely Induces formation of autophagosome-like double-membrane liposomes [112] Summary of Interactions between proteins from positive strand RNA viruses and host autophagy machinery. abstract: Autophagy is an evolutionarily conserved process central to host metabolism. Among its major functions are conservation of energy during starvation, recycling organelles, and turnover of long-lived proteins. Besides, autophagy plays a critical role in removing intracellular pathogens and very likely represents a primordial intrinsic cellular defence mechanism. More recent findings indicate that it has not only retained its ability to degrade intracellular pathogens, but also functions to augment and fine tune antiviral immune responses. Interestingly, viruses have also co-evolved strategies to manipulate this pathway and use it to their advantage. Particularly intriguing is infection-dependent activation of autophagy with positive stranded (+)RNA virus infections, which benefit from the pathway without succumbing to lysosomal degradation. In this review we summarise recent data on viral manipulation of autophagy, with a particular emphasis on +RNA viruses and highlight key unanswered questions in the field that we believe merit further attention. url: https://www.sciencedirect.com/science/article/pii/S1084952118302222 doi: 10.1016/j.semcdb.2019.07.013 id: cord-002085-e7xwb03g author: Yamashita, Akifumi title: DGV: Dengue Genographic Viewer date: 2016-06-07 words: 2456.0 sentences: 130.0 pages: flesch: 48.0 cache: ./cache/cord-002085-e7xwb03g.txt txt: ./txt/cord-002085-e7xwb03g.txt summary: We constructed a DENV database containing the serotype, genotype, year and country/region of collection by collecting all publically available DENV sequence information from the National Center for Biotechnology Information (NCBI) and assigning genotype information. DGV also assigns the serotype and genotype to a user-specified sequence by performing a homology search against the curated DENV database, and shows its homologous sequences with the geographical position and year of collection. The second database is the Dengue virus genotyping database 2 (Yamashita et al., 2013) , which provides a summary table containing the DENV serotype/genotype, year and country of collection and accession number. The Dengue Virus Resource 3 facilitates the retrieval of DENV sequences deposited in GenBank according to serotype, disease symptom, host, region/country, genome region, and collection and/or release data (Resch et al., 2009) . DGV provides a search engine for the assignment of the DENV serotype, genotype, and origin country according to the most homologous sequence on the basis of a blastn search against the DENV database. abstract: Dengue viruses (DENVs) and their vectors are widely distributed throughout the tropical and subtropical regions of the world. An autochthonous case of DENV was reported in Tokyo, Japan, in 2014, for the first time in 70 years. A comprehensive database of DENV sequences containing both serotype and genotype data and epidemiological data is crucial to trace DENV outbreak isolates and promptly respond to outbreaks. We constructed a DENV database containing the serotype, genotype, year and country/region of collection by collecting all publically available DENV sequence information from the National Center for Biotechnology Information (NCBI) and assigning genotype information. We also implemented the web service Dengue Genographic Viewer (DGV), which shows the geographical distribution of each DENV genotype in a user-specified time span. DGV also assigns the serotype and genotype to a user-specified sequence by performing a homology search against the curated DENV database, and shows its homologous sequences with the geographical position and year of collection. DGV also shows the distribution of DENV-infected entrants to Japan by plotting epidemiological data from the Infectious Agents Surveillance Report (IASR), Japan. This overview of the DENV genotype distribution may aid in planning for the control of DENV infections. DGV is freely available online at: (https://gph.niid.go.jp/geograph/dengue/content/genomemap). url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4894901/ doi: 10.3389/fmicb.2016.00875 id: cord-297662-slmlhqnb author: Yap, Sally S. L. title: Dengue Virus Glycosylation: What Do We Know? date: 2017-07-25 words: 11116.0 sentences: 526.0 pages: flesch: 48.0 cache: ./cache/cord-297662-slmlhqnb.txt txt: ./txt/cord-297662-slmlhqnb.txt summary: In this review, we seek to provide a comprehensive summary of the current knowledge on protein glycosylation in DENV, and its role in virus biogenesis, host cell receptor interaction and disease pathogenesis. Since high mannose binding DC-SIGN interacts only with N67 glycans on the viral surface (Pokidysheva et al., 2006) and N153-glycan is dispensable for virus production in mosquito and mammalian cells (Bryant et al., 2007) , this suggests that N153 glycans may serve a distinct function from N67 glycans in DEN pathogenesis possibly via interaction with an unknown fucose binder or act as a viral glycan shield. Finally, N153 deglycosylated (N153 − ) DENV mutant displayed reduced infectivity (10-fold lower) in both mammalian and mosquito cells compared to WT, possibly due to impaired virus entry process (Lee et al., 1997; Hacker et al., 2009) , whereby loss of the N153-glycan affected the conformational stability of E proteins and led to premature exposure of the fusion peptide (Yoshii et al., 2013) . N-linked glycosylation of dengue virus NS1 protein modulates secretion, cell-surface expression, hexamer stability, and interactions with human complement abstract: In many infectious diseases caused by either viruses or bacteria, pathogen glycoproteins play important roles during the infection cycle, ranging from entry to successful intracellular replication and host immune evasion. Dengue is no exception. Dengue virus glycoproteins, envelope protein (E) and non-structural protein 1 (NS1) are two popular sub-unit vaccine candidates. E protein on the virion surface is the major target of neutralizing antibodies. NS1 which is secreted during DENV infection has been shown to induce a variety of host responses through its binding to several host factors. However, despite their critical role in disease and protection, the glycosylated variants of these two proteins and their biological importance have remained understudied. In this review, we seek to provide a comprehensive summary of the current knowledge on protein glycosylation in DENV, and its role in virus biogenesis, host cell receptor interaction and disease pathogenesis. url: https://doi.org/10.3389/fmicb.2017.01415 doi: 10.3389/fmicb.2017.01415 id: cord-328661-spxgox52 author: Yu, Jianhai title: Epidemiological and Evolutionary Analysis of Dengue-1 Virus Detected in Guangdong during 2014: Recycling of Old and Formation of New Lineages date: 2019-08-05 words: 5834.0 sentences: 314.0 pages: flesch: 50.0 cache: ./cache/cord-328661-spxgox52.txt txt: ./txt/cord-328661-spxgox52.txt summary: The lack of sufficient epidemiological data and evidence on the local mosquito-borne DENV emphasizes the importance of studying the molecular evolutionary features and establishing a well-established phylogenetic tree for dengue prevention and control in Guangdong. Since 1990, however, DENV1 has been mainly isolated from the infected cases, and its continued existence in Guangdong Province indicated that endemic infectious agents of dengue may be circulating locally. With the epidemiological data since 2005 supplied by the Guangdong Provincial CDC, we studied phylogenetics, molecular characteristics, and epidemiology to strengthen the foundational research of DENV1 for the prevention of large-scale dengue epidemics, providing preventive and control measures of DF with important evidence. Based on representative strains of the E gene in lineages of the 2014 outbreak, as well as the molecular evolution database, we analyzed molecular characterization and possibility of local circulation for DENV1 since 2005 in Guangdong. abstract: The incidence of dengue is increasing in Guangdong, China, with the largest outbreak to date in 2014. Widespread awareness of epidemiological and molecular characteristics of the dengue virus (DENV) is required. In 2014, we isolated the virus from patients and sequenced its genome. The sequences of DENV isolated from Guangdong and other countries screened since 2005 were studied to establish molecular evolutionary databases along with epidemiological data to explore its epidemiological, phylogenetic, and molecular characteristics. Causes underlying the occurrence of the dengue epidemic included importation and localization of the virus. The number of indigenous cases significantly exceeded that of imported cases. Dengue virus 1 is the most important serotype and caused the long-term epidemic locally. Based on the data available since 2005, DENV1 was divided into three genotypes (I, IV, and V). Only genotypes I and V were detected in 2014. In 2014, an epidemic involving old lineages of DENV1 genotype V occurred after 2 years of silence. The genotype was previously detected from 2009 to 2011. Genotype I, which caused recent epidemics, demonstrated a continuation of new lineages, and a predictive pattern of molecular evolution since 2005 among the four lineages was present. The DENV isolated from Guangdong was closely related to those causing large-scale epidemics in neighboring countries, suggesting the possibility of its import from these countries. The lack of sufficient epidemiological data and evidence on the local mosquito-borne DENV emphasizes the importance of studying the molecular evolutionary features and establishing a well-established phylogenetic tree for dengue prevention and control in Guangdong. url: https://www.ncbi.nlm.nih.gov/pubmed/31392945/ doi: 10.4269/ajtmh.18-0951 id: cord-330743-o11d0aa1 author: Yu, Xi title: Broad-spectrum virucidal activity of bacterial secreted lipases against flaviviruses, SARS-CoV-2 and other enveloped viruses date: 2020-05-25 words: 4470.0 sentences: 245.0 pages: flesch: 54.0 cache: ./cache/cord-330743-o11d0aa1.txt txt: ./txt/cord-330743-o11d0aa1.txt summary: Herein, we identified 2 secreted bacterial lipases from a Chromobacterium bacterium, named Chromobacterium antiviral effector-1 (CbAE-1) and CbAE-2, with a broad-spectrum virucidal activity against dengue virus (DENV), Zika virus (ZIKV), severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), human immunodeficiency virus (HIV) and herpes simplex virus (HSV). Incubation of the culture supernatant but not the bacterial lysates resulted in significant suppression of DENV ( Figure 1B ) and ZIKV ( Figure 1C ) infectivity in Vero cells, indicating that an extracellular effector(s) secreted by Csp_BJ was responsible for viral inhibition. DENV, ZIKV, HSV-1 and SARS-CoV-2 virus stocks were diluted to 50 plaque-forming units (pfu) per ml and incubated untreated or with a serial dilution of the CbAEs in five-fold steps at 37°C for 1 hr before being added onto Vero cell monolayers for 2 hr of infection. abstract: Viruses are the major aetiological agents of acute and chronic severe human diseases that place a tremendous burden on global public health and economy; however, for most viruses, effective prophylactics and therapeutics are lacking, in particular, broad-spectrum antiviral agents. Herein, we identified 2 secreted bacterial lipases from a Chromobacterium bacterium, named Chromobacterium antiviral effector-1 (CbAE-1) and CbAE-2, with a broad-spectrum virucidal activity against dengue virus (DENV), Zika virus (ZIKV), severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), human immunodeficiency virus (HIV) and herpes simplex virus (HSV). The CbAEs potently blocked viral infection in the extracellular milieu through their lipase activity. Mechanistic studies showed that this lipase activity directly disrupted the viral envelope structure, thus inactivating infectivity. A mutation of CbAE-1 in its lipase motif fully abrogated the virucidal ability. Furthermore, CbAE-2 presented low toxicity in vivo and in vitro, highlighting its potential as a broad-spectrum antiviral drug. url: https://doi.org/10.1101/2020.05.22.109900 doi: 10.1101/2020.05.22.109900 id: cord-277547-2vim1wno author: Zandi, Keivan title: Antiviral activity of four types of bioflavonoid against dengue virus type-2 date: 2011-12-28 words: 4381.0 sentences: 247.0 pages: flesch: 52.0 cache: ./cache/cord-277547-2vim1wno.txt txt: ./txt/cord-277547-2vim1wno.txt summary: In the present study, antiviral activity of four types of bioflavonoid against dengue virus type -2 (DENV-2) in Vero cell was evaluated. Daidzein showed a weak anti-dengue activity with IC(50 )= 142.6 μg mL(-1 )when the DENV-2 infected cells were treated after virus adsorption. Although there was no significant direct virucidal activity against DENV-2 by quercetin, continuous treatment of cells from 5 h before virus infection up to 4 days post-infection exhibited anti-dengue activity with IC 50 = 28.9 μg mL -1 (Figure 3a) . There was no significant change in the antiviral activity of daidzein when cells were treated continuously from 5 h before virus infection up to 4 days post infection comparing to its anti-dengue activity for postadsorption treatment (Figure 1 ). To investigate which of the many flavonoids could affect DENV infection, in the present study, we examined the potential effects of quercetin, naringin, hesperetin and daidzein on dengue virus infection of Vero cells. abstract: BACKGROUND: Dengue is a major mosquito-borne disease currently with no effective antiviral or vaccine available. Effort to find antivirals for it has focused on bioflavonoids, a plant-derived polyphenolic compounds with many potential health benefits. In the present study, antiviral activity of four types of bioflavonoid against dengue virus type -2 (DENV-2) in Vero cell was evaluated. Anti-dengue activity of these compounds was determined at different stages of DENV-2 infection and replication cycle. DENV replication was measured by Foci Forming Unit Reduction Assay (FFURA) and quantitative RT-PCR. Selectivity Index value (SI) was determined as the ratio of cytotoxic concentration 50 (CC(50)) to inhibitory concentration 50 (IC(50)) for each compound. RESULTS: The half maximal inhibitory concentration (IC(50)) of quercetin against dengue virus was 35.7 μg mL(-1 )when it was used after virus adsorption to the cells. The IC(50 )decreased to 28.9 μg mL(-1 )when the cells were treated continuously for 5 h before virus infection and up to 4 days post-infection. The SI values for quercetin were 7.07 and 8.74 μg mL(-1), respectively, the highest compared to all bioflavonoids studied. Naringin only exhibited anti-adsorption effects against DENV-2 with IC(50 )= 168.2 μg mL(-1 )and its related SI was 1.3. Daidzein showed a weak anti-dengue activity with IC(50 )= 142.6 μg mL(-1 )when the DENV-2 infected cells were treated after virus adsorption. The SI value for this compound was 1.03. Hesperetin did not exhibit any antiviral activity against DENV-2. The findings obtained from Foci Forming Unit Reduction Assay (FFURA) were corroborated by findings of the qRT-PCR assays. Quercetin and daidzein (50 μg mL(-1)) reduced DENV-2 RNA levels by 67% and 25%, respectively. There was no significant inhibition of DENV-2 RNA levels with naringin and hesperetin. CONCLUSION: Results from the study suggest that only quercetin demonstrated significant anti-DENV-2 inhibitory activities. Other bioflavonoids, including daidzein, naringin and hesperetin showed minimal to no significant inhibition of DENV-2 virus replication. These findings, together with those previously reported suggest that select group of bioflavonoids including quercetin and fisetin, exhibited significant inhibitory activities against dengue virus. This group of flavonoids, flavonol, could be investigated further to discover the common mechanisms of inhibition of dengue virus replication. url: https://www.ncbi.nlm.nih.gov/pubmed/22201648/ doi: 10.1186/1743-422x-8-560 id: cord-025181-eg108wcd author: Zheng, Zhihang title: Establishment of Murine Infection Models with Biological Clones of Dengue Viruses Derived from a Single Clinical Viral Isolate date: 2020-05-25 words: nan sentences: nan pages: flesch: nan cache: txt: summary: abstract: Dengue virus (DENV) is a single-stranded RNA virus transmitted by mosquitoes in tropical and subtropical regions. It causes dengue fever, dengue hemorrhagic fever and dengue shock syndrome in patients. Each year, 390 million people are estimated to be infected by four serotypes of dengue virus, creating a great burden on global public health and local economy. So far, no antiviral drug is available for dengue disease, and the newly licensed vaccine is far from satisfactory. One large obstacle for dengue vaccine and drug development is the lack of suitable small animal models. Although some DENV infection models have been developed, only a small number of viral strains can infect immunodeficient mice. In this study, with biologically cloned viruses from a single clinical isolate, we have established two mouse models of DENV infection, one is severe lethal infection in immunocompromised mice, and the other resembles self-limited disease manifestations in Balb/c mice with transient blockage of type I IFN responses. This study not only offers new small animal models of dengue viral infection, but also provides new viral variants for further investigations on dengue viral pathogenesis. url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7246292/ doi: 10.1007/s12250-020-00229-y id: cord-353241-ityhcak7 author: Zhu, Hanliang title: IoT PCR for pandemic disease detection and its spread monitoring date: 2020-01-15 words: 4300.0 sentences: 208.0 pages: flesch: 54.0 cache: ./cache/cord-353241-ityhcak7.txt txt: ./txt/cord-353241-ityhcak7.txt summary: Considerable effort has been invested in the development of portable, user-friendly, and cost-effective systems for point-of-care (POC) diagnostics, which could also create an Internet of Things (IoT) for healthcare via a global network. Connecting the easy to use and cost-effective POC devices providing the DENV diagnoses via a mobile network would create an Internet of Things (IoT) [15] for healthcare [16, 17] , an essential tool to tackle any infectious disease outbreak. Prior to testing on an IoT PCR device, we verified the master mix performance and its values of critical threshold (C T ) and the melting temperature (T M ) using a commercial real-time PCR system (Supplementary Section A) beginning with a hot start at 95°C for 30 s followed by 40 cycles of PCR amplification consisting of DNA denaturation at 95°C for 8 s, primer annealing at 60°C for 30 s, and DNA sequence elongation at 72°C for 10 s, then followed by melting curve analysis (MCA) from 72°C to 95°C. abstract: During infectious disease outbreaks, the centers for disease control need to monitor particular areas. Considerable effort has been invested in the development of portable, user-friendly, and cost-effective systems for point-of-care (POC) diagnostics, which could also create an Internet of Things (IoT) for healthcare via a global network. However, at present IoT based on a functional POC instrument is not available. Here we show a fast, user-friendly, and affordable IoT system based on a miniaturized polymerase chain reaction device. We demonstrated the system’s capability by amplification of complementary deoxyribonucleic acid (cDNA) of the dengue fever virus. The resulting data were then automatically uploaded via a Bluetooth interface to an Android-based smartphone and then wirelessly sent to a global network, instantly making the test results available anywhere in the world. The IoT system presented here could become an essential tool for healthcare centers to tackle infectious disease outbreaks identified either by DNA or ribonucleic acid. url: https://www.sciencedirect.com/science/article/pii/S0925400519312973 doi: 10.1016/j.snb.2019.127098 id: cord-300648-ixiam7qr author: Zhu, Xun title: IFITM3‐containing exosome as a novel mediator for anti‐viral response in dengue virus infection date: 2014-08-30 words: nan sentences: nan pages: flesch: nan cache: txt: summary: abstract: Interferon‐inducible transmembrane proteins 1, 2 and 3 (IFITM1, IFITM2 and IFITM3) have recently been identified as potent antiviral effectors that function to suppress the entry of a broad range of enveloped viruses and modulate cellular tropism independent of viral receptor expression. However, the antiviral effect and mechanisms of IFITMs in response to viral infections remain incompletely understood and characterized. In this work, we focused our investigation on the function of the extracellular IFITM3 protein. In cell models of DENV‐2 infection, we found that IFITM3 contributed to both the baseline and interferon‐induced inhibition of DENV entry. Most importantly, our study for the first time demonstrated the presence of IFITM‐containing exosome in the extracellular environment, and identified an ability of cellular exosome to intercellularly deliver IFITM3 and thus transmit its antiviral effect from infected to non‐infected cells. Thus, our findings provide new insights in the basic mechanisms underlying the actions of IFITM3, which might lead to future development of exosome‐mediated anti‐viral strategies using IFITM3 as a therapeutic agent. Conceivably, variations in the basal and inducible levels of IFITMs, as well as in intracellular and extracellular levels of IFITMs, might predict the severity of dengue virus infections among individuals or across species. url: https://www.ncbi.nlm.nih.gov/pubmed/25131332/ doi: 10.1111/cmi.12339 ==== make-pages.sh questions [ERIC WAS HERE] ==== make-pages.sh search /data-disk/reader-compute/reader-cord/bin/make-pages.sh: line 77: /data-disk/reader-compute/reader-cord/tmp/search.htm: No such file or directory Traceback (most recent call last): File "/data-disk/reader-compute/reader-cord/bin/tsv2htm-search.py", line 51, in with open( TEMPLATE, 'r' ) as handle : htm = handle.read() FileNotFoundError: [Errno 2] No such file or directory: '/data-disk/reader-compute/reader-cord/tmp/search.htm' ==== make-pages.sh topic modeling corpus Zipping study carrel