id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-000049-rl7sdzd7	Lee, David	Detection of genetically modified organisms (GMOs) using isothermal amplification of target DNA sequences	2009-02-02		.txt	text/plain	2899	141	55	Here we have applied the loop-mediated isothermal amplification (LAMP) method to amplify GMO-related DNA sequences, 'internal' commonly-used motifs for controlling transgene expression and event-specific (plant-transgene) junctions. Moreover, the sensitivity and ability to amplify targets, even with a high background of DNA, here demonstrated, highlights the advantages of this isothermal amplification when applied for GMO detection. Here we assess the LAMP protocol for the detection of GMOs using primers that target event-specific sequences for transgenic MS8 and RF3 oilseed rape (Brassica napus L.) and generic GMO sequences such as the cauliflower mosaic virus 35S promoter (P-35S) and the promoter and terminator for the nopaline synthase gene (P-nos and T-nos, respectively) from Agrobacterium spp. LAMP sensitivity was assessed by the detection of ten RoundUp Ready™ GMO targets in a background of 100 ng of genomic oilseed rape DNA (Figure 3 ).	./cache/cord-000049-rl7sdzd7.txt	./txt/cord-000049-rl7sdzd7.txt