id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-000436-k1hwh640	Amidi, Maryam	Antigen-expressing immunostimulatory liposomes as a genetically programmable synthetic vaccine	2010-10-26		.txt	text/plain	5886	313	47	To show proof-of-concept for this artificial cell-based platform, a bacterial in vitro transcription and translation system together with a gene construct encoding the model antigen β-galactosidase were entrapped inside multilamellar liposomes. Vaccination studies in mice showed that such antigen-expressing immunostimulatory liposomes (AnExILs) elicited higher specific humoral immune responses against the produced antigen (β-galactosidase) than control vaccines (i.e. AnExILs without genetic input, liposomal β-galactosidase or pDNA encoding β-galactosidase). Here, we show that AnExILs expressing b-galactosidase are well tolerated after i.m. injection and were capable of inducing strong systemic immune responses, which were superior to that of liposomal DNA or protein vaccines encoding the same antigen. Characterization of AnExILs and liposomes loaded with b-galactosidase and/or pDNA Cell-free protein synthesis was used to transcribe and translate the lacZ gene encoding for E. AnExILs combine antigen-production, delivery and adjuvanticity in one system, making them more potent in inducing antibody responses compared to liposomal DNA vaccines as shown here.	./cache/cord-000436-k1hwh640.txt	./txt/cord-000436-k1hwh640.txt