id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-001090-qg2r691d	Twin, Jimmy	The Potential of Metatranscriptomics for Identifying Screening Targets for Bacterial Vaginosis	2013-09-27		.txt	text/plain	3944	201	44	BACKGROUND: The ribosomal RNA content of a sample collected from a woman with bacterial vaginosis (BV) was analysed to determine the active microbial community, and to identify potential targets for further screening. Prevotella amnii was chosen as an example target, being the most metabolically active species present in the single patient with BV, and was found to be detected at a high concentration by qPCR in 31% of cohort with BV, with an association with both oral and penile-vaginal sex. An amplicon-based metagenomic library was generated from the extracted DNA using the universal bacterial PCR primers 27F and 338R that target the V1-V2 hypervariable regions of the 16S rRNA gene as previously described [15] . Comparison of cDNA to DNA from Nugent 10 patient All of the predominant bacteria with .1% total abundance identified in the cDNA library were also detected using the 16S rRNA gene amplicon-based approach on extracted DNA.	./cache/cord-001090-qg2r691d.txt	./txt/cord-001090-qg2r691d.txt