id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-001111-qqmj4v0u	Liu, Chengyu	Strategies for Designing Transgenic DNA Constructs	2013-03-08		.txt	text/plain	8122	382	47	Finally, the strategies for conducting each of the major types of transgenic research are discussed, including gene overexpression, promoter characterization, cell-lineage tracing, mutant complementation, expression of double or multiple transgenes, siRNA knockdown, and conditional and inducible systems. Generating a typical transgenic construct involves assembling three basic DNA elements: (1) a promoter and/or enhancer which confers the desired spatial and temporal pattern of transgene expression; (2) the gene to be transcribed, which may or may not encode a protein; and (3) a transcription termination or polyadenylation signal sequence to stop transcription and enable 3 ¢ end processing. BACs can be used for driving expression of Cre and FLP recombinase genes in desired tissues, but as will be discussed in Subheading 4.6 , the commonly used BAC vectors already contain loxP sites, which can be problematic when crossbred to fl oxed mouse lines.	./cache/cord-001111-qqmj4v0u.txt	./txt/cord-001111-qqmj4v0u.txt