id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-001406-huz0tpmi	Kersting, Sebastian	Multiplex isothermal solid-phase recombinase polymerase amplification for the specific and fast DNA-based detection of three bacterial pathogens	2014-02-18		.txt	text/plain	4671	255	41	title: Multiplex isothermal solid-phase recombinase polymerase amplification for the specific and fast DNA-based detection of three bacterial pathogens The isothermal RPA was applied to amplify specific target sequences from the pathogens Neisseria gonorrhoeae, Salmonella enterica and methicillin-resistant Staphylococcus aureus (MRSA) using genomic DNA. A possible alternative to the PCR are isothermal nucleic acid amplification techniques which are carried out at a single temperature throughout the entire reaction using different mechanisms e.g. the strand-displacement activity of certain polymerases or the addition of further proteins used in the natural replication processes [4] . To our knowledge this highly multiplex pathogen detection is the first combination of isothermal RPA and microarray technology and offers new possibilities for the development of point-of-care testing devices for nucleic acids. (i) The excess of reverse primer in the reaction and the subsequent polymerase elongation leads to a preferred amplification of a single strand antisense DNA strand which can hybridize to the target specific probe structure on the surface.	./cache/cord-001406-huz0tpmi.txt	./txt/cord-001406-huz0tpmi.txt