id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-012461-v8d91fdo	Marnissi, Boutheina	Generation of ssDNA aptamers as diagnostic tool for Newcastle avian virus	2020-08-13		.txt	text/plain	6108	304	54	Top aptameric sequences were used to develop a sandwich enzymatic linked aptamer assay (ELAA) for rapid and sensitive detection of NDV in farm samples. Following optimization of the sandwich ELAA method, the results demonstrated that both selected aptamers Apt_NDV01 and Apt_NDV03 with dissociation constant values of 31 nM and 78.1 nM, respectively, showed the highest specificity and affinity for NDV detection. The output of the SELEX was amplified by symmetric (S1 Fig in S1 File) and asymmetric PCR, and the final PCR products were incubated with the virus immobilized on a nitrocellulose membrane, using an immune-blot test to monitor the enrichment of target-binding aptamers. The specificity of the five selected aptamers, Apt_NDV01-05, against NDV was evaluated by testing their affinities against various avian viruses besides NDV-LaSota vaccine strain, including H120-IBV, IBDV-Gomboro, 1133 reovirus, avian influenza-H9N2, and a naïve library which was used as a negative control.	./cache/cord-012461-v8d91fdo.txt	./txt/cord-012461-v8d91fdo.txt