id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-031565-mos619wp	Troedsson, Christofer	Quantification of copepod gut content by differential length amplification quantitative PCR (dla-qPCR)	2009-02-01		.txt	text/plain	4215	211	47	Recently, molecular based methods have demonstrated that DNA from prey species can be used to identify zooplankton gut contents, and further, quantitative gut content estimates by quantitative PCR (qPCR) assays targeted to the 18S rRNA gene have been used to estimate feeding rates in appendicularians and copepods. The assay utilizes multiple primer sets that amplify different sized fragments of the prey 18S rRNA gene and, based on the differential amplification of these fragments, the degree of digestion is estimated and corrected for. Recently, our group developed a PCR based assay for detection of prey content in the gut of the calanoid copepod Calanus finmarchicus (Nejstgaard et al. We refer to this method as ''differential length amplification quantitative polymerase chain reaction'' (dla-qPCR), and by amplifying different sized fragments of the same prey target gene extracted from copepod guts, we hypothesize that it would be possible to generate a digestion profile of a target prey species consumed by a copepod.	./cache/cord-031565-mos619wp.txt	./txt/cord-031565-mos619wp.txt