id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-262660-t1ndfn2l	Hass, Kenneth N.	Integrated Micropillar Polydimethylsiloxane Accurate CRISPR Detection System for Viral DNA Sensing	2020-10-13		.txt	text/plain	5219	317	54	This miniaturized and fully packed IMPACT chip demonstrates sensitive and accurate DNA detection within 120 min and paves ways to the next-generation point-of-care diagnostics, responding to emerging and deadly pathogen outbreaks. On the solid surface, reporter probes do not require a quencher since they are only measured in the liquid phase after degradation; thus, the fluorescent signal will be largely reduced without the target DNA present in the assay. 19, 20 Here, we present a fully enclosed Integrated Micropillar Polydimethylsiloxane Accurate CRISPR deTection (IMPACT) system for nucleic acid target detection on the solid-surface of polydimethylsiloxane (PDMS) utilizing CRISPR-Cas12a. Leveraging the high activity of CRISPR-Cas12a enzyme and the ability of micropillars to bind more reporter probes, we successfully detected a double-stranded DNA target without background issues. The extended surface provided by high-aspect ratio micropillars significantly increases the reporter probe binding capacity.	./cache/cord-262660-t1ndfn2l.txt	./txt/cord-262660-t1ndfn2l.txt