id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-262733-icnkx1rx	Daneluz, Larissa O.	Efficiency and cell viability implications using tip type electroporation in zebrafish sperm cells	2020-07-13		.txt	text/plain	4961	254	46	Sperm kinetics parameters were assessed using Computer Assisted Semen Analysis (CASA) and cell integrity, reactive oxygen species (ROS), mitochondrial functionality and transfection rate were evaluated by flow cytometry. increased DNA uptake by sperm cells, some problems are associated with the conventional cuvette type electroporation, such as low transfection rate, mosaic gene expression, and negative effects on cell viability [9] . The objective of this study was to demonstrate the feasibility of tip type electroporation in Danio rerio sperm, allowing its further use in SMGT, showing a protocol that provide high transfection efficiency, with minimal sideeffects on sperm cells. Cell integrity, membrane fluidity, mitochondrial functionality, concentration of reactive oxygen species (ROS) and total of sperm-bound exogenous DNA were evaluated by flow cytometry (Attune® Acoustic Focusing Cytometer, Applied Biosystems, USA) as previously described [30] . Here, transfection rate of fish spermatozoa was positively affected for tip type electroporation, increasing the number of cells containing exogenous DNA.	./cache/cord-262733-icnkx1rx.txt	./txt/cord-262733-icnkx1rx.txt