id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-270082-byxd4o4m	Doheny, Kimberly Floy	Identification of essential components of the S. cerevisiae kinetochore	1993-05-21		.txt	text/plain	9918	556	54	Readthrough Assay and Secondary Screen of the ctf Collection When transcription from a strong promoter is initiated toward a CEN DNA sequence, the mitotic segregational function of the centromere is destroyed (Hill and Bloom, 1987) without disruption of its 180-220 bp nuclease protected region (Bloom et al., 1984; Hill and Bloom, 1987) , indicating that at least some of the kinetochore complex remains intact. To test the hypothesis that a CfN DNA-protein complex was responsible for the transcriptional block, we replaced the wild-type CENG sequence with a CEN6 sequence containing a single-nucleotide point mutation (CDEIII-15C) in the central element of the palindrome of CDEIII (CCG). Of 34 cff mutants screened (see Experimental Procedures for list), 7 were identified as putative kinetochore mutants because they produced an intermediate level of blue colony color between the levels of the CTF+ strains carrying the wild-type and mutant CEN reporters.	./cache/cord-270082-byxd4o4m.txt	./txt/cord-270082-byxd4o4m.txt