id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-279827-921kvrrz	Murata, Takayuki	Growth behavior of bovine herpesvirus-1 in permissive and semi-permissive cells	1999-06-11		.txt	text/plain	7150	368	65	MDBK or HmLu-1 cells in 35 mm dishes were incubated with 1.0× 10 5 , 1.0 ×10 4 or 1.0 × 10 3 plaque forming unit (pfu) of BHV-1 at a multiplicity of infection (moi) of 0.1, 0.01 or 0.001, respectively, at 4°C for 2 h to allow the virus to be adsorbed into the cells. MDBK or HmLu-1 cells in 60 mm dishes were infected with BHV-1/RSV/p32 at 4°C for 1 h, washed three times with ice-cold PBS and incubated at 37°C in the medium containing 400 mg/ml phosphonoacetic acid (PAA). Confluent monolayer cultures of MDBK cells or HmLu-1 cells were infected with BHV-1 at an moi of 5 and at 3, 6, 12, 24, and 48 h p.i., DNA was extracted as described in Section 2. The MDBK or HmLu-1 cells were infected with BHV-1/RSV/p32 at 4°C for 1 h, washed extensively with cold PBS and incubated with medium at 37°C for 0, 1, 2, and 5 h.	./cache/cord-279827-921kvrrz.txt	./txt/cord-279827-921kvrrz.txt