id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-339522-jm2xpn1w	Sharma, Nidhi	Recombinase‐Based Isothermal Amplification of Nucleic Acids with Self‐Avoiding Molecular Recognition Systems (SAMRS)	2014-09-10		.txt	text/plain	3873	219	55	In contrast, with the primers containing SAMRS components, clean amplification products (amplicons) of the correct identity (as judged by length) were produced if the reaction mixture contained the target DNA (Figure 3 A, lane 2) . Real-time amplification with STD primers resulted in a nonspecific signal that does not depend on the amount of target template added to the reaction mixtures (Figure 4 B, left) . Once again, STD primers amplified unwanted products in the negative control (Figure 5 A) , giving a background signal that obscured the desired amplicons when lower concentrations of viral RNA template was used in the RT-RPA reaction. These experiments show that, at least for these three cases, adding SAMRS nucleotides to primers used in RPA isothermal amplification eliminates assay artifacts, allows for readout by using real-time fluorescence signal, and increases the robustness of the assay with respect to using different target sequences.	./cache/cord-339522-jm2xpn1w.txt	./txt/cord-339522-jm2xpn1w.txt