id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-342629-mzi0krja	Wu, Q.	An Activated GOPS‐poly‐L‐Lysine‐ Coated Glass Surface for the Immobilization of 60mer Oligonucleotides	2005-11-16		.txt	text/plain	2086	118	49	To explore a method for enhancing the immobilization and hybridization efficiency of oligonucleotides on DNA microarrays, conventional protocols of poly‐L‐lysine coating were modified by means of surface chemistry, namely, the slides were prepared by the covalently coupling of poly‐L‐lysine to a glycidoxy‐modified glass surface. The modified slides were then used to print microarrays for the detection of the SARS coronavirus by means of 60mer oligonucleotide probes. With the development of the DNA microarray technology, many reports on the modification of glass surfaces and the immobilization of oligonucleotides onto solid supports by covalent linkage have appeared [8±12]. In this paper, the process of slide surface chemistry based on conventional protocols for poly-L-lysine coating was improved [6, 13] in order to immobilize 60mer oligonucleotides by deposition technology. Oligo microarrays printed on an activated GOPS-PLL slide could undergo hybridization stripping cycles for at least three cycles. Preparation of SARS 60mer oligonucleotide probes for microarray printing	./cache/cord-342629-mzi0krja.txt	./txt/cord-342629-mzi0krja.txt