id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-002935-jq1xumrh	Postnikova, Elena	Testing therapeutics in cell-based assays: Factors that influence the apparent potency of drugs	2018-03-22		.txt	text/plain	6226	315	56	Here, we describe variable conditions tested during the development of our cell-based drug screen assays designed to identify compounds with anti-Ebola virus activity using established cell lines and human primary cells. The effect of multiple assay readouts and variable assay conditions, including virus input, time of infection, and the cell passage number, were compared, and the impact on the effective concentration for 50% and/ or 90% inhibition (EC(50), EC(90)) was evaluated using the FDA-approved compound, toremifene citrate. The CELIA was compared to the fluorescent assay (detected by regular plate reader or the HCI system) by testing the efficacy of toremifene citrate against EBOV infection in Huh 7 cells with a range of MOIs (0.1, 0.3, 1, and 3 ) at three different time points (24, 48 and 72 hpi) . The established CELIA was used to compare anti-EBOV activity of toremifene citrate in 3 different cell types, Vero E6, Huh 7 and MDMs using an MOI of 0.5 and a time point of 48 h (Fig 7) .	./cache/cord-002935-jq1xumrh.txt	./txt/cord-002935-jq1xumrh.txt