id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-102206-mb0qcd0b	Seymour, Elif	Configurable Digital Virus Counter on Robust Universal DNA Chips	2020-10-22		.txt	text/plain	5898	284	52	Here, we demonstrate real-time multiplexed virus detection by applying DNA-directed antibody immobilization technique to a single-particle interferometric reflectance imaging sensor (SP-IRIS). We also show that this method can be modified to produce a single-step, homogeneous assay format by mixing the antibody-DNA conjugates with the virus sample in solution phase prior to flowing in the microfluidic cartridge, eliminating the antibody immobilization step. 16 SP-IRIS has been utilized for detecting viruses in complex media using antibody microarrays by imaging chips both dry (dried after sample incubation and wash steps) and in liquid using disposable microfluidic cartridges. To demonstrate the feasibility of using the homogeneous assay in combination with the passive flow cartridge and to compare its performance to the directly immobilized antibody assay, an SP-IRIS chip was printed with anti-EBOV mAb, A' probe, and a negative DNA sequence, and washed as described previously.	./cache/cord-102206-mb0qcd0b.txt	./txt/cord-102206-mb0qcd0b.txt