id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-267941-nrluar4e	Park, Eun-Mee	Production of Ebola virus-like particles in Drosophila melanogaster Schneider 2 cells	2018-08-23		.txt	text/plain	2162	127	55	In this study, we generated recombinant virus-like particles (VLPs) against family Filoviridae, genus Ebolavirus, species Zaire ebolavirus, strain Makona (EBOV) in Drosophila melanogaster Schneider 2 (S2) cells using the EBOV Makona. eVLPs were produced by sucrose gradient ultracentrifugation of S2 cells transfected with EBOV Makona genes, and production of VLPs was confirmed by immunoblot analysis. In a previous study, VP2/6 double-layered rotavirus-like particles containing Wa capsid protein were produced in S2 cells transformed with a bicistronic expression system consisting of encephalomyocarditis virus (EMCV)-derived internal ribosomal entry site (IRES) element (Lee et al., 2011) . As shown in Fig. 1A , we confirmed that Ebola viral proteins were efficiently expressed in EBOV Makona-transfected S2 cells. Collectively, our findings suggested that the eVLPs produced by the S2 cell system in this study may be useful for the development of efficient VLP-based vaccines against EBOV.	./cache/cord-267941-nrluar4e.txt	./txt/cord-267941-nrluar4e.txt