id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-272576-ez731lif	Wada, Yoshiko	Directional and reoccurring sequence change in zoonotic RNA virus genomes visualized by time-series word count	2016-11-03		.txt	text/plain	5641	273	50	To face the world-wide threats caused by zoonotic RNA viruses, which suddenly cause serious outbreaks by invasion from nonhuman hosts and mutate rapidly, we must understand the molecular evolutionary changes in their genome sequences extensively by innovating various technologies, including those used in big data analyses, e.g., large-scale word count. The present time-series analysis on influenza A genomes showed that the 20-mers exhibiting the most evident directional changes observed in common for three different subtypes corresponded to several influenza A siRNAs, which were experimentally validated. The present study focused on time-series changes, but not on data variations caused largely by random mutations and sequencing uncertainty, and therefore, average characteristics of strains isolated in a similar period were analyzed, summing up the sequences isolated in one month and calculating an averaged mononucleotide composition for each month (Fig. 1b) .	./cache/cord-272576-ez731lif.txt	./txt/cord-272576-ez731lif.txt