id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-279152-yh7x7w2q	Ndungo, Esther	A Single Residue in Ebola Virus Receptor NPC1 Influences Cellular Host Range in Reptiles	2016-03-30		.txt	text/plain	5230	243	49	Here, we investigated the role of the intracellular filovirus receptor, Niemann-Pick C1 (NPC1) as a molecular determinant of Ebola virus (EBOV) host range at the cellular level. Analysis of panels of viper-human NPC1 chimeras and point mutants allowed us to identify a single amino acid residue in NPC1, at position 503, that bidirectionally influenced both its binding to EBOV GP and its viral receptor activity in cells. We also found that NPC1 could influence the cellular host range of filoviruses-human NPC1 conferred susceptibility to filovirus entry and infection when expressed in the nonpermissive reptilian cell line VH-2, derived from a Russell's viper (Daboia russellii) (22) . These findings provide additional evidence that NPC1-encoded residue 503 influences the cellular host range of EBOV at the level of virus-receptor recognition and raise the possibility that sequence differences at this position influence the susceptibility of reptiles to filovirus infection in nature.	./cache/cord-279152-yh7x7w2q.txt	./txt/cord-279152-yh7x7w2q.txt