id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-294342-7ycgd0h7	Markosyan, Ruben M.	Induction of Cell-Cell Fusion by Ebola Virus Glycoprotein: Low pH Is Not a Trigger	2016-01-05		.txt	text/plain	11740	548	56	On the question of low pH, we found that activation of cathepsins by acidity is the sole cause for augmentation of fusion: if EBOV GP on the cell surface is artificially cleaved by thermolysin in the presence of cathepsin inhibitors, the extent of fusion is independent of pH. We used thermolysin-treated effector cells to maximize cleavage of EBOV GP and found that adding CPZ either 30, 45, or 60 min after reneutralization did not induce any dye spread above that already observed, strongly indicating that a negligible percentage of cells were hemifused, but not fused, at any given time. The extent of fusion was greater when the inhibitor was not added (control): this indicates that delivery to the cell surface of EBOV GP cleaved by endosomal cathepsin (subsequent to thermolysin treatment) significantly contributes to fusion.	./cache/cord-294342-7ycgd0h7.txt	./txt/cord-294342-7ycgd0h7.txt