id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-296399-vvbjulm9	Brinkmann, Constantin	The glycoprotein of vesicular stomatitis virus promotes release of virus-like particles from tetherin-positive cells	2017-12-07		.txt	text/plain	7009	340	44	Vesicular stomatitis virus (VSV) release from infected cells is inhibited by the interferon (IFN)-inducible antiviral host cell factor tetherin (BST-2, CD317). Here, we show that the viral glycoprotein (VSV-G) antagonizes tetherin in transfected cells, although with reduced efficiency as compared to the HIV-1 Vpu protein. At 1 h post infection, the inoculum was removed and the cells were transfected with expression plasmids for VSV-N, -P and -L (see above) as well as the respective, plasmid-encoded VSV Ã anti-genome (the genome is preceded by a T7 promotor sequence and followed by a hepatitis delta virus ribozyme and a T7 terminator sequence for cytoplasmic production of negative-sensed viral genomes that are template for transcription and genome replication) using Lipofectamine2000 (ThermoFisher Scientific, Dreiech) as transfection reagent. In order to analyze tetherin antagonism by VSV proteins, we employed a previously reported virus-like particle (VLP) release assay, which measures release of HIV-Gag-based VLPs from transfected 293T cells and its blockade by tetherin [27, 32] .	./cache/cord-296399-vvbjulm9.txt	./txt/cord-296399-vvbjulm9.txt