id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-305653-f9vqn96o	Schmidt, Rebecca	Generation of therapeutic antisera for emerging viral infections	2018-10-05		.txt	text/plain	7902	377	46	Animal-origin purified IgG 27 and IgG fragment preparations are still routinely used as antivenoms, and even though immunization of horses with inactivated EBOV failed to yield effective therapeutic antisera, 28 equine hyperimmune sera produced using EBOV virus-like particles (VLPs) conferred protection against lethal challenge in rodents. To determine the importance of this aspect for the induction of functional antibodies, we compared adjuvanted EBOV VLPs with vector vaccines based on the replication-deficient modified vaccinia Ankara virus (MVA) that expresses either the GP protein of the Zaire EBOV Guéckédou strain alone or in combination with VP40 (unpublished data), and the replication-competent VSVΔG/EBOV-GP. Even though the neutralizing antibodies were again only detected after the first boost in the group immunized with adjuvanted EBOV VLPs, titers ultimately reached levels around 100 (Fig. 2b) , illustrating that de novo protein expression is not required for efficient induction of functional antibodies.	./cache/cord-305653-f9vqn96o.txt	./txt/cord-305653-f9vqn96o.txt