id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-322748-a5131tv9	Yates, Mary K.	Flex-nucleoside analogues – Novel therapeutics against filoviruses	2017-06-15		.txt	text/plain	1492	78	53	Most recently GS-5734, a monophosphoramidate prodrug adenosine analogue which targets EBOV RNA-dependent RNA polymerase (RdRp), exhibited very potent activity against both EBOV and MARV, 17, 18 further demonstrating the potential for finding effective nucleoside inhibitors of filoviruses. After the successful synthesis of the three Flex-analogues 1, 2, and 3, the compounds were screened against a panel of filoviruses including EBOV, MARV, and SUDV, as well as other hemorrhagic fever viruses such as Lassa and Rift Valley Fever. The second series of assays utilized Huh7 cells infected with recombinant reporter EBOV, Lassa, and Rift Valley Fever viruses. Within this study we found that both compounds 1 and 3 exhibited antiviral activity against a recombinant reporter EBOV in Huh7 cells, though surprisingly the McGuigan prodrug was 10-fold less potent (EC 50 = 2.2 ± 0.3 lM and 27.2 ± 2.2 lM respectively).	./cache/cord-322748-a5131tv9.txt	./txt/cord-322748-a5131tv9.txt