id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-007480-grndfx7b	Koopmans, M.	Seroepidemiology of Breda virus in cattle using ELISA	2002-11-13		.txt	text/plain	3354	176	55	Two direct blocking enzyme linked immunosorbent assays (ELISA) for the detection of antibodies to Breda virus in sera of cattle were compared. This "Breda virus" (BRV) is morphologically and antigenically different from known bovine viruses and causes diarrhea in gnotobiotic and colostrum-deprived calves (Woode et al., 1982) . In the first approach (consecutive method) a 1:400 dilution of the BRV2 antigen preparation, purified from calf feces was added to the coated wells in ELISA buffer 1 (PBS supplemented with 0.35 M NaC1, 1 mM EDTA pH 7.5 and 0.05% Tween 80 ). When using the consecutive method more animals were found negative for antibodies to BRV2 or had low blocking percentages (Table 1 ) . In Fig. 3 , the percentages of animals with antibodies to BRV ( >~ 20% blocking, corresponding to a serum titer of/> 30 ) are shown for different age groups (n--244).	./cache/cord-007480-grndfx7b.txt	./txt/cord-007480-grndfx7b.txt