id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-007497-nn5l5rai	Garcia-Sanchez, J.	Survey of rotavirus infection in a dairy herd: comparison between polycrylamide gel electrophoresis and two commercial tests	2002-11-13		.txt	text/plain	3578	165	54	Faecal samples taken from 15 cows before and after calving as well as faeces taken from their calves daily from birth to two weeks of life were tested for rotavirus by polyacrylamide gel electrophoresis (PAGE) and compared with an ELISA and a latex agglutination commercial test. Various methods have been developed for rapid detection of rotaviruses in faecal samples; these include electron microscopy (considered for a long time as the reference method), immune electron microscopy, counter immuno-electrophoresis, complement fixation, fluorescent antibody tests, different immunoassays using antibodies labelled with radioisotopes or enzymes (ELISA), latex agglutination and polyacrylamide gel electrophoresis (PAGE). All the faeces samples (120 from cows and 240 from calves) were tested for the presence of rotaviruses by polyacrylamide gel electrophoresis (PAGE), a commercial ELISA kit and a commercial latex agglutination kit. Another three discrepancies were detected in which the samples were positive by PAGE and negative by both ELISA and latex agglutination and all of them corresponded to two calves with a discontinuous shedding pattern (calf no.	./cache/cord-007497-nn5l5rai.txt	./txt/cord-007497-nn5l5rai.txt