id author title date pages extension mime words sentences flesch summary cache txt cord-009784-kxa68zbc Bolton, Jessica S. Comparison of ELISA with electro-chemiluminescence technology for the qualitative and quantitative assessment of serological responses to vaccination 2020-04-17 .txt text/plain 6166 286 47 The PfCSP-FL protein is comprised of 26 Tyr -127 Asp linked to 207 Pro -383 Ser [4] ; "Repeat" is a Keywords: Serology, Vaccine, Antigen, Multiplex, Antigenic competition, ELISA, Electro-chemiluminescence 32-mer peptide representing the central Repeat region (NANP 8 ); C-term is a recombinant protein representing the C-terminal fragment (AA 207-383); Pf16 is an epitope within the C-terminus that has been used as a functional marker when evaluating anti-CSP antibodies induced by vaccination [4, 7, 8] . To establish a multiplex assay using an ECLIA platform, several parameters (i.e., antigen coating concentration, antigenic competition between closely related antigens, sample dilutions) were optimized and the performance of the assay determined in regards to specificity, linearity, and throughput. ./cache/cord-009784-kxa68zbc.txt ./txt/cord-009784-kxa68zbc.txt