id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-011212-ovjdzyxv	Pan, Qing	Development and application of a novel ELISA for detecting antibodies against group I fowl adenoviruses	2019-12-14		.txt	text/plain	3884	223	47	Since 2015, outbreaks of hepatitis-hydropericardium syndrome (HPS) caused by a novel genotype of fowl adenovirus 4 (FAdV-4) infection have created serious economic losses in China. In one recent study, recombinant fiber-based indirect ELISA was used to detect serum samples from chickens experimentally inoculated with different FAdV-1 or FAdV-4 strains (Feichtner et al. A recombinant hexon-based single serum dilution ELISA was also developed to measure the hexon-specific antibodies against FAdV-4 in sera of chickens (Rajasekhar and Roy 2014) . In this study, we developed a group-specific and sensitive ELISA based on the novel genotype of FAdV-4 for detecting antibodies against twelve FAdV-I serotypes. The common ELISA developed in our study was applied to detect serum samples from SPF chickens inoculated with inactivated FAdV-1, FAdV-4, and FAdV-8a, and showed high sensitivity for all three FAdV hypervirulent serotypes. Furthermore, the ELISA showed higher sensitivity in detecting serum samples of HPS caused by the novel FAdV-4 genotype that has recently emerged in China.	./cache/cord-011212-ovjdzyxv.txt	./txt/cord-011212-ovjdzyxv.txt