id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-011840-neowfhwg	Liu, Weixiao	Development of a sensitive monoclonal antibody-based sandwich ELISA to detect Vip3Aa in genetically modified crops	2020-03-05		.txt	text/plain	3920	232	52	title: Development of a sensitive monoclonal antibody-based sandwich ELISA to detect Vip3Aa in genetically modified crops OBJECTIVES: To develop a sensitive monoclonal antibody-based sandwich enzyme-linked immunosorbent assay (ELISA) to detect Vip3Aa in genetically modified (GM) crops and their products. A sensitive monoclonal antibody-based sandwich ELISA was developed to detect Vip3Aa in GM crops and their products. These antibodies were then employed to develop a sandwich ELISA for sensitive, direct and convenient measurement of the Vip3Aa concentration in GM crops and their products. To the best of our knowledge, this is the first quantitative monoclonal antibody-based ELISA method reported for the sensitive detection of Vip3Aa proteins. In this study, we describe a sensitive monoclonal antibody-based ELISA method for the detection of Vip3Aa in GM crops and their products. Development of monoclonal antibodies against Cry1Ab protein from Bacillus thuringiensis and their application in an ELISA for detection of transgenic Bt-maize	./cache/cord-011840-neowfhwg.txt	./txt/cord-011840-neowfhwg.txt