id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-255390-dvp0luxe	Jones, R. D	Capture ELISA and flow cytometry methods for toxicologic assessment following immunization and cyclophosphamide challenges in beagles	2000-04-10		.txt	text/plain	4834	237	39	Abstract The purpose of this subacute 22-day study was to evaluate methods for canine circulating immunoglobulins (IgM, IgG, and IgE) and select Band T-lymphocyte populations (CD4-helpers, CD8-suppressors, pan-T and pan-B) for immunotoxicity testing using an organ system (concordance) approach. The flow cytometry method was acceptable for measuring select canine lymphocyte populations and detecting the expected decrease in B cells due to cyclophosphamide treatment. The purpose of this study was to evaluate immunoglobulins (IgM, IgG, IgE) by ELISA and lymphocytes (CD4-helpers, CD8-suppressors, pan-T CD5 and pan-B CD21) by flow cytometry for detecting adverse effects in dogs administered positive control challenges to the immune system. The next day following each immunization, serum samples were taken for analysis of IgG, IgM and IgE, along with clinical chemistry, hematology, urinalysis, and flow cytometry on day 8, to evaluate possible influences of repeated immunization.	./cache/cord-255390-dvp0luxe.txt	./txt/cord-255390-dvp0luxe.txt