id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-262762-mgegswvn	Callebaut, P.	Enzyme-linked immunosorbent assay for the detection of the coronavirus-like agent and its antibodies in pigs with porcine epidemic diarrhea	1982-09-30		.txt	text/plain	3605	179	50	Abstract An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of the coronavirus-like agent in feces of pigs naturally affected with porcine epidemic diarrhea (PED) or experimentally infected with the CV777 isolate. Control fecal samples, used to determine the limit between positive and negative absorbance values in ELISA, consisted of 25 non-diarrheal specimens obtained from conventional pigs of all ages. A total of 62 serum samples, to be examined for antibody by ELISA blocking, were collected from four CDCD piglets and seven conventional experimental fattening pigs prior to inoculation with CV777 and at various intervals between 7 and 81 days thereafter. To compare the sensitivity of EM and ELISA for the detection of CVLA, the specimens of fecal material and intestinal contents obtained from experimentally infected CDCD piglets and experimental fattening pigs as described in the section "Specimens from experimental pigs", were examined for coronavirus by EM.	./cache/cord-262762-mgegswvn.txt	./txt/cord-262762-mgegswvn.txt