id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-322529-3xn5v54s	Rodák, L.	Verification of Sensitivity and Specificity of Group A Rotavirus Detection in Piglets Faeces with Monoclonal Blocking ELISA Methods	2004-06-30		.txt	text/plain	3215	196	48	title: Verification of Sensitivity and Specificity of Group A Rotavirus Detection in Piglets Faeces with Monoclonal Blocking ELISA Methods Selected competitive blocking ELISA (CB‐ELISA) and electron microscopy (EM) were used for examination of 194 field faecal samples of piglets affected with diarrhoea. The sensitivity and specificity of the CB‐ELISA was verified both by inclusion of control samples containing transmissible gastroenteritis virus (TGEV) and porcine epidemic diarrhoea virus (PEDV) in each analysis and by comparative examination of samples with the commercial ELISA kit. Sensitivity comparison of three variants of the blocking ELISA method of rotavirus A detection were performed by box titrations in microtitre plate wells pre-coated with binding antibodies. Sensitivity of the CB-ELISA method and DAS-ELISA kit was compared by examination of faecal sample of experimentally infected piglet twofold diluted 2· to 1024·. By examination of positive faecal sample twofold diluted 2· to 1024·, at least 10 times higher sensitivity of CB-ELISA method was demonstrated (Table 2) .	./cache/cord-322529-3xn5v54s.txt	./txt/cord-322529-3xn5v54s.txt