id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-335591-r0x8yaqj	Ohnishi, Kazuo	Establishment and Characterization of Monoclonal Antibodies Against SARS Coronavirus	2007-11-28		.txt	text/plain	3126	242	67	The hybridomas produce monoclonal antibodies that recognize viral component molecules, including the spike protein (S) and the nucleocapsid protein (N), enabling the immunological detection of SARS-CoV by immunofluorescence staining, immunoblot, or an antigen-capture ELISA system. Based on clinical experience, several options have been considered in the quest to develop the capacity to accurately diagnose SARS-CoV infection, including molecular biology techniques and serological tests such as antigen-capture ELISA assay and immunofluorescence assay to detect virus-infected cells in respiratory swabs (3-7) . These mAbs enable the general immunological detection of SARS-CoV by methods such as immunofluorescent staining, immunoblotting, and immunohistology, in addition to the construction of a highly sensitive antigen-capture sandwich ELISA (6). The UV-inactivated purified SARS-CoV samples (see Note 1), which are serially diluted with 1% OVA/PBS-Tween, are added to the wells and incubated for 1 h at room temperature	./cache/cord-335591-r0x8yaqj.txt	./txt/cord-335591-r0x8yaqj.txt