id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-300489-gzcb6uqw	Martinez, Mitzi L.	Detection of feline infectious peritonitis virus infection in cell cultures and peripheral blood mononuclear leukocytes of experimentally infected cats using a biotinylated cDNA probe	1993-03-31		.txt	text/plain	3704	203	52	title: Detection of feline infectious peritonitis virus infection in cell cultures and peripheral blood mononuclear leukocytes of experimentally infected cats using a biotinylated cDNA probe Abstract A dot blot hybridization assay, using a biotinylated cDNA probe, was able to detect feline infectious peritonitis virus (FIPV) RNA in Felis catus whole fetus (fcwf-4) cells infected with the FIPV isolates DF2, 79-1146, UCDI, and UCD2. In an in vivo study, the probe was used to detect FIPV RNA in peripheral blood mononuclear leukocytes (PBML) isolated at various post-infection days (PID) from cats experimentally infected with the FIP-producing coronavirus isolate FIPV-79-1146 or FIPV-DF2. In this study, we describe a dot blot hybridization procedure, using a biotinylated recombinant cDNA probe complementary to a major portion of the N protein gene, to detect FIPV in feline cell cultures and PBML isolated from cats infected experimentally with the virulent FIP virus isolate 79-1146 or DF2.	./cache/cord-300489-gzcb6uqw.txt	./txt/cord-300489-gzcb6uqw.txt