id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-282968-kjvvoveq	Qu, Renjun	Selection of reference genes for the quantitative real-time PCR normalization of gene expression in Isatis indigotica fortune	2019-03-25		.txt	text/plain	6267	315	47	indigotica, and evaluated their expression stabilities in different tissues (root, stem, leaf, and petiole) and leaves exposed to three abiotic treatments (low-nitrogen, ABA, and MeJA) using geNorm, NormFinder, BestKeeper, and comprehensive RefFind algorithms. TIP41 as the optimal reference gene for low-nitrogen stress and MeJA treatment, while ACT had the highest ranking for ABA treatment and CYP was the most suitable for different tissues. Excel-based tools, such as geNorm [34] , NormFinder [35] and BestKeeper [36] , have been developed to select the most suitable reference genes from a set of biological samples under investigation to be used in an expression stability analysis. indigotica (SRR1051997) to determine appropriate reference genes for qRT-PCR normalization in different plant tissues, and under low-nitrogen stress and exposure to hormonal stimuli (ABA and MeJA). The results were normalized using the selected stable reference genes (singly or in combination) and the unstable genes in sample sets across treatment with a N, b different tissues, c ABA, and d MeJA.	./cache/cord-282968-kjvvoveq.txt	./txt/cord-282968-kjvvoveq.txt