id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-011794-ejoufvvj	Binder, Florian	Isolation and characterization of new Puumala orthohantavirus strains from Germany	2020-04-23		.txt	text/plain	5972	309	51	Additionally, glycoprotein precursor (GPC)-derived virus-like particles of a German PUUV sequence allowed the generation of monoclonal antibodies that allowed the reliable detection of the isolated PUUV strain in the immunofluorescence assay. Finally, the reactivity of the isolates was determined with novel monoclonal antibodies raised against PUUV GPC VLPs. Bank voles were trapped in spring 2019 in the PUUV endemic region around Osnabrück following a standard snap trapping protocol [25, 26] . Dissection on site and inoculation of VeroE6 and bank vole MGN-2-R cells with homogenized lung samples resulted after three blind passages in four potential isolates that were detected by a novel PUUV RT-qPCR (Table S1 , Fig. 1) . Reactivity of novel PUUV GPC-specific monoclonal antibodies with hantavirus-infected VeroE6 cells in immunofluorescence assay (IFA). In conclusion, the PUUV isolate described here replicates in a bank vole cell line and its N and GPC proteins can be detected by specific monoclonal antibodies.	./cache/cord-011794-ejoufvvj.txt	./txt/cord-011794-ejoufvvj.txt