id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-255465-sc1yzzsn	Krasteva, Gabriela	Caveolin-1 and -2 in airway epithelium: expression and in situ association as detected by FRET-CLSM	2006-08-11		.txt	text/plain	5663	307	52	METHODS: Western blotting, real-time quantitative PCR analysis of abraded tracheal epithelium and laser-assisted microdissection combined with subsequent mRNA analysis were used to examine the expression of cav-1 and cav-2, two major caveolar coat proteins, in rat tracheal epithelium. Immunoreactivities for cav-1 and for cav-2 were co-localized in the cell membrane of the basal cells and basolaterally in the ciliated epithelial cells of large airways of rat and human. To address the molecular composition of caveolae, we determined the molecular association of cav-1α and cav-2 in tracheal epithelial cells in tissue sections by double-labeling indirect immunofluorescence combined with confocal laser scanning microscopy (CLSM) and fluorescence resonance energy transfer (FRET) analysis. RT-PCR analysis of total mRNA isolated from rat lungs and abraded tracheal epithelial cells revealed expression of cav-1 and cav-2. Conventional indirect double-labeling immunofluorescence with subsequent FRET-CLSM analysis was conducted to determine whether cav-1 and cav-2 are in close apposition in airway epithelial cells in situ, thereby indicating an association of both proteins and formation of hetero-oligomers.	./cache/cord-255465-sc1yzzsn.txt	./txt/cord-255465-sc1yzzsn.txt