id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-326223-q6e60nf8	Gembardt, Florian	Organ-specific distribution of ACE2 mRNA and correlating peptidase activity in rodents	2005-02-16		.txt	text/plain	3948	238	54	Biochemical analysis revealed that angiotensin-converting enzyme related carboxy-peptidase (ACE2) cleaves angiotensin (Ang) II to Ang-(1–7), a heptapeptide identified as an endogenous ligand for the G protein-coupled receptor Mas. No data are currently available that systematically describe ACE2 distribution and activity in rodents. Therefore, we analyzed the ACE2 expression in different tissues of mice and rats on mRNA (RNase protection assay) and protein levels (immunohistochemistry, ACE2 activity, western blot). Although ACE2 mRNA in both investigated species showed the highest expression in the ileum, the mouse organ exceeded rat ACE2, as also demonstrated in the kidney and colon. Using a commercial polyclonal antibody in western blot for the quantification of protein levels in mouse and rat tissues (Fig. 4) a pattern completely different from RNA expression and ACE2 activity was found.	./cache/cord-326223-q6e60nf8.txt	./txt/cord-326223-q6e60nf8.txt