id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-316814-9fv9xrln	Li, Hong-Ye	Use of GFP to Investigate Expression of Plant-Derived Vaccines	2009		.txt	text/plain	2817	204	53	Agroinfiltration is a more recent technique that can be applied to investigate transient expression in plant cells by which an Agrobacterium liquid culture is infiltrated into intact plant leaves (7) . By simple infiltration of Agrobacterium cells carrying appropriate gene constructs into tobacco plants leaves, transient expression assays can be performed within 3 days without using expensive instruments or complicated procedures. Two days after agroinfiltration, expression and subcellular localization of the GFP fusion proteins in tobacco leaves can be determined by simple observation under fluorescence or confocal laser scanning microscopy. 7. Generate plasmid pCV12 (Fig. 1 ) or similar nuclear transformation vector for expression of a fusion protein consisting of the SARS-CoV S1 and GFP. Production of tobacco leaves transiently expressing a protein fusion consisting of the SARS-CoV S1 protein fused with the GFP was carried out using Agrobacterium-mediated transformation with plasmid pCV12.	./cache/cord-316814-9fv9xrln.txt	./txt/cord-316814-9fv9xrln.txt