id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-319842-4mnaicki	Jackson, William T	Subversion of Cellular Autophagosomal Machinery by RNA Viruses	2005-04-26		.txt	text/plain	6936	320	39	Infection of human cells with poliovirus induces the proliferation of double-membraned cytoplasmic vesicles whose surfaces are used as the sites of viral RNA replication and whose origin is unknown. Here, we explore the role of several constituents of autophagosome machinery in poliovirus-and rhinovirusinfected cells by monitoring: the presence of autophagosomal protein LC3 in virally induced vesicles, the acquisition of colocalization of LC3 and LAMP1 in virally infected cells, the viral induction of punctate structures that stain with monodansylcadaverine (MDC), and the effects of perturbing the autophagosomal pathway pharmacologically and via RNA interference on intracellular and extracellular virus yield. To determine whether the autophagosome-like membranes induced during poliovirus infection perform an antiviral function or facilitate viral replication, we tested the effect on poliovirus yield of pretreating H1-HeLa cells with either tamoxifen or rapamycin, known inducers of autophagy.	./cache/cord-319842-4mnaicki.txt	./txt/cord-319842-4mnaicki.txt