id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-332881-mkm4ygh6	Kirchhoff, Jana	Three viruses of the bovine respiratory disease complex apply different strategies to initiate infection	2014-02-18		.txt	text/plain	6369	339	49	We investigated the susceptibility of bovine airway epithelial cells (BAEC) to infection by the three major viruses associated with the BRDC: bovine respiratory syncytial virus (BRSV), bovine herpesvirus type 1 (BHV-1) and bovine parainfluenza virus type 3 (BPIV3). For this purpose, two culture systems for well-differentiated BAEC were used: the air-liquid interface (ALI) system, where filter-grown BAEC differentiate into a pseudostratified respiratory epithelium and precision-cut lung slices (PCLS) where BAEC are maintained in the original tissue organisation. We have reported recently that well-differentiated respiratory epithelial cells are susceptible to infection by BPIV3 whereas they are rather resistant to infection by BRSV [7] . BPIV3 efficiently infected the airway epithelial cells using sialic acids on the apical surface as a receptor determinant for virus entry from the apical side. When BHV-1-GFP was applied to the apical side of the well-differentiated airway epithelial cells, infected cells were detected only occasionally.	./cache/cord-332881-mkm4ygh6.txt	./txt/cord-332881-mkm4ygh6.txt