id sid tid token lemma pos cord-103868-iwpiti2h 1 1 key key JJ cord-103868-iwpiti2h 1 2 : : : cord-103868-iwpiti2h 1 3 cord-103868-iwpiti2h cord-103868-iwpiti2h NN cord-103868-iwpiti2h 1 4 authors author NNS cord-103868-iwpiti2h 1 5 : : : cord-103868-iwpiti2h 1 6 Harrison Harrison NNP cord-103868-iwpiti2h 1 7 , , , cord-103868-iwpiti2h 1 8 Angela Angela NNP cord-103868-iwpiti2h 1 9 R. R. NNP cord-103868-iwpiti2h 1 10 ; ; : cord-103868-iwpiti2h 1 11 Moseley Moseley NNP cord-103868-iwpiti2h 1 12 , , , cord-103868-iwpiti2h 1 13 Gregory Gregory NNP cord-103868-iwpiti2h 1 14 W. W. NNP cord-103868-iwpiti2h 1 15 title title NN cord-103868-iwpiti2h 1 16 : : : cord-103868-iwpiti2h 1 17 The the DT cord-103868-iwpiti2h 1 18 Ebola Ebola NNP cord-103868-iwpiti2h 1 19 virus virus NN cord-103868-iwpiti2h 1 20 interferon interferon VBD cord-103868-iwpiti2h 1 21 antagonist antagonist NN cord-103868-iwpiti2h 2 1 VP24 VP24 NNP cord-103868-iwpiti2h 2 2 undergoes undergo VBZ cord-103868-iwpiti2h 2 3 active active JJ cord-103868-iwpiti2h 2 4 nucleocytoplasmic nucleocytoplasmic JJ cord-103868-iwpiti2h 2 5 trafficking trafficking NN cord-103868-iwpiti2h 2 6 date date NN cord-103868-iwpiti2h 3 1 : : : cord-103868-iwpiti2h 3 2 2020 2020 CD cord-103868-iwpiti2h 3 3 - - HYPH cord-103868-iwpiti2h 3 4 08 08 CD cord-103868-iwpiti2h 3 5 - - HYPH cord-103868-iwpiti2h 3 6 11 11 CD cord-103868-iwpiti2h 3 7 journal journal NN cord-103868-iwpiti2h 3 8 : : : cord-103868-iwpiti2h 4 1 bioRxiv biorxiv IN cord-103868-iwpiti2h 4 2 DOI DOI NNP cord-103868-iwpiti2h 4 3 : : : cord-103868-iwpiti2h 5 1 10.1101/2020.08.10.245563 10.1101/2020.08.10.245563 CD cord-103868-iwpiti2h 6 1 sha sha NNP cord-103868-iwpiti2h 6 2 : : : cord-103868-iwpiti2h 7 1 f70ebaae9cd9e020abf8ffa0f828558cb80c9b9d f70ebaae9cd9e020abf8ffa0f828558cb80c9b9d NNP cord-103868-iwpiti2h 7 2 doc_id doc_id NNP cord-103868-iwpiti2h 8 1 : : : cord-103868-iwpiti2h 8 2 103868 103868 CD cord-103868-iwpiti2h 8 3 cord_uid cord_uid NNS cord-103868-iwpiti2h 8 4 : : : cord-103868-iwpiti2h 8 5 iwpiti2h iwpiti2h . cord-103868-iwpiti2h 9 1 Viral viral JJ cord-103868-iwpiti2h 9 2 interferon interferon NN cord-103868-iwpiti2h 10 1 ( ( -LRB- cord-103868-iwpiti2h 10 2 IFN IFN NNP cord-103868-iwpiti2h 10 3 ) ) -RRB- cord-103868-iwpiti2h 10 4 antagonist antagonist NN cord-103868-iwpiti2h 10 5 proteins protein NNS cord-103868-iwpiti2h 10 6 mediate mediate VBP cord-103868-iwpiti2h 10 7 evasion evasion NN cord-103868-iwpiti2h 10 8 of of IN cord-103868-iwpiti2h 10 9 IFN IFN NNP cord-103868-iwpiti2h 10 10 - - HYPH cord-103868-iwpiti2h 10 11 mediated mediate VBN cord-103868-iwpiti2h 10 12 innate innate JJ cord-103868-iwpiti2h 10 13 immunity immunity NN cord-103868-iwpiti2h 10 14 and and CC cord-103868-iwpiti2h 10 15 are be VBP cord-103868-iwpiti2h 10 16 often often RB cord-103868-iwpiti2h 10 17 multifunctional multifunctional JJ cord-103868-iwpiti2h 10 18 , , , cord-103868-iwpiti2h 10 19 having have VBG cord-103868-iwpiti2h 10 20 distinct distinct JJ cord-103868-iwpiti2h 10 21 roles role NNS cord-103868-iwpiti2h 10 22 in in IN cord-103868-iwpiti2h 10 23 viral viral JJ cord-103868-iwpiti2h 10 24 replication replication NN cord-103868-iwpiti2h 10 25 processes process NNS cord-103868-iwpiti2h 10 26 . . . cord-103868-iwpiti2h 11 1 Functions function NNS cord-103868-iwpiti2h 11 2 of of IN cord-103868-iwpiti2h 11 3 the the DT cord-103868-iwpiti2h 11 4 Ebola Ebola NNP cord-103868-iwpiti2h 11 5 virus virus NN cord-103868-iwpiti2h 11 6 ( ( -LRB- cord-103868-iwpiti2h 11 7 EBOV EBOV NNP cord-103868-iwpiti2h 11 8 ) ) -RRB- cord-103868-iwpiti2h 12 1 IFN IFN NNP cord-103868-iwpiti2h 12 2 antagonist antagonist NN cord-103868-iwpiti2h 12 3 VP24 VP24 NNP cord-103868-iwpiti2h 12 4 include include VBP cord-103868-iwpiti2h 12 5 nucleocapsid nucleocapsid NNP cord-103868-iwpiti2h 12 6 assembly assembly NN cord-103868-iwpiti2h 12 7 during during IN cord-103868-iwpiti2h 12 8 cytoplasmic cytoplasmic JJ cord-103868-iwpiti2h 12 9 replication replication NN cord-103868-iwpiti2h 12 10 and and CC cord-103868-iwpiti2h 12 11 inhibition inhibition NN cord-103868-iwpiti2h 12 12 of of IN cord-103868-iwpiti2h 12 13 IFN IFN NNP cord-103868-iwpiti2h 12 14 - - HYPH cord-103868-iwpiti2h 12 15 activated activate VBN cord-103868-iwpiti2h 12 16 signalling signalling NN cord-103868-iwpiti2h 12 17 by by IN cord-103868-iwpiti2h 12 18 STAT1 stat1 NN cord-103868-iwpiti2h 12 19 . . . cord-103868-iwpiti2h 13 1 For for IN cord-103868-iwpiti2h 13 2 the the DT cord-103868-iwpiti2h 13 3 latter latter JJ cord-103868-iwpiti2h 13 4 , , , cord-103868-iwpiti2h 13 5 VP24 VP24 NNP cord-103868-iwpiti2h 13 6 prevents prevent VBZ cord-103868-iwpiti2h 13 7 STAT1 stat1 POS cord-103868-iwpiti2h 13 8 nuclear nuclear JJ cord-103868-iwpiti2h 13 9 import import NN cord-103868-iwpiti2h 13 10 via via IN cord-103868-iwpiti2h 13 11 competitive competitive JJ cord-103868-iwpiti2h 13 12 binding binding NN cord-103868-iwpiti2h 13 13 to to IN cord-103868-iwpiti2h 13 14 nuclear nuclear JJ cord-103868-iwpiti2h 13 15 import import NN cord-103868-iwpiti2h 13 16 receptors receptor NNS cord-103868-iwpiti2h 13 17 ( ( -LRB- cord-103868-iwpiti2h 13 18 karyopherins karyopherins NNP cord-103868-iwpiti2h 13 19 ) ) -RRB- cord-103868-iwpiti2h 13 20 . . . cord-103868-iwpiti2h 14 1 Many many JJ cord-103868-iwpiti2h 14 2 viral viral JJ cord-103868-iwpiti2h 14 3 proteins protein NNS cord-103868-iwpiti2h 14 4 , , , cord-103868-iwpiti2h 14 5 including include VBG cord-103868-iwpiti2h 14 6 proteins protein NNS cord-103868-iwpiti2h 14 7 from from IN cord-103868-iwpiti2h 14 8 viruses virus NNS cord-103868-iwpiti2h 14 9 with with IN cord-103868-iwpiti2h 14 10 cytoplasmic cytoplasmic JJ cord-103868-iwpiti2h 14 11 replication replication NN cord-103868-iwpiti2h 14 12 cycles cycle NNS cord-103868-iwpiti2h 14 13 , , , cord-103868-iwpiti2h 14 14 interact interact VBP cord-103868-iwpiti2h 14 15 with with IN cord-103868-iwpiti2h 14 16 the the DT cord-103868-iwpiti2h 14 17 trafficking traffic VBG cord-103868-iwpiti2h 14 18 machinery machinery NN cord-103868-iwpiti2h 14 19 to to TO cord-103868-iwpiti2h 14 20 undergo undergo VB cord-103868-iwpiti2h 14 21 nucleocytoplasmic nucleocytoplasmic JJ cord-103868-iwpiti2h 14 22 transport transport NN cord-103868-iwpiti2h 14 23 , , , cord-103868-iwpiti2h 14 24 with with IN cord-103868-iwpiti2h 14 25 key key JJ cord-103868-iwpiti2h 14 26 roles role NNS cord-103868-iwpiti2h 14 27 in in IN cord-103868-iwpiti2h 14 28 pathogenesis pathogenesis NN cord-103868-iwpiti2h 14 29 . . . cord-103868-iwpiti2h 15 1 Despite despite IN cord-103868-iwpiti2h 15 2 established establish VBN cord-103868-iwpiti2h 15 3 karyopherin karyopherin NNP cord-103868-iwpiti2h 15 4 interaction interaction NN cord-103868-iwpiti2h 15 5 , , , cord-103868-iwpiti2h 15 6 the the DT cord-103868-iwpiti2h 15 7 nuclear nuclear JJ cord-103868-iwpiti2h 15 8 trafficking trafficking NN cord-103868-iwpiti2h 15 9 profile profile NN cord-103868-iwpiti2h 15 10 of of IN cord-103868-iwpiti2h 15 11 VP24 VP24 NNP cord-103868-iwpiti2h 15 12 has have VBZ cord-103868-iwpiti2h 15 13 not not RB cord-103868-iwpiti2h 15 14 been be VBN cord-103868-iwpiti2h 15 15 investigated investigate VBN cord-103868-iwpiti2h 15 16 . . . cord-103868-iwpiti2h 16 1 We -PRON- PRP cord-103868-iwpiti2h 16 2 find find VBP cord-103868-iwpiti2h 16 3 that that IN cord-103868-iwpiti2h 16 4 VP24 VP24 NNP cord-103868-iwpiti2h 16 5 becomes become VBZ cord-103868-iwpiti2h 16 6 strongly strongly RB cord-103868-iwpiti2h 16 7 nuclear nuclear JJ cord-103868-iwpiti2h 16 8 following follow VBG cord-103868-iwpiti2h 16 9 overexpression overexpression NN cord-103868-iwpiti2h 16 10 of of IN cord-103868-iwpiti2h 16 11 karyopherin karyopherin NN cord-103868-iwpiti2h 16 12 or or CC cord-103868-iwpiti2h 16 13 inhibition inhibition NN cord-103868-iwpiti2h 16 14 of of IN cord-103868-iwpiti2h 16 15 nuclear nuclear JJ cord-103868-iwpiti2h 16 16 export export NN cord-103868-iwpiti2h 16 17 pathways pathway NNS cord-103868-iwpiti2h 16 18 . . . cord-103868-iwpiti2h 17 1 Molecular molecular JJ cord-103868-iwpiti2h 17 2 mapping mapping NN cord-103868-iwpiti2h 17 3 indicates indicate VBZ cord-103868-iwpiti2h 17 4 that that IN cord-103868-iwpiti2h 17 5 cytoplasmic cytoplasmic JJ cord-103868-iwpiti2h 17 6 localisation localisation NN cord-103868-iwpiti2h 17 7 of of IN cord-103868-iwpiti2h 17 8 VP24 VP24 NNP cord-103868-iwpiti2h 17 9 depends depend VBZ cord-103868-iwpiti2h 17 10 on on IN cord-103868-iwpiti2h 17 11 a a DT cord-103868-iwpiti2h 17 12 CRM1-dependent CRM1-dependent NNP cord-103868-iwpiti2h 17 13 nuclear nuclear JJ cord-103868-iwpiti2h 17 14 export export NN cord-103868-iwpiti2h 17 15 sequence sequence NN cord-103868-iwpiti2h 17 16 at at IN cord-103868-iwpiti2h 17 17 the the DT cord-103868-iwpiti2h 17 18 VP24 VP24 NNP cord-103868-iwpiti2h 17 19 C C NNP cord-103868-iwpiti2h 17 20 - - HYPH cord-103868-iwpiti2h 17 21 terminus terminus NN cord-103868-iwpiti2h 17 22 . . . cord-103868-iwpiti2h 18 1 Nuclear nuclear JJ cord-103868-iwpiti2h 18 2 export export NN cord-103868-iwpiti2h 18 3 is be VBZ cord-103868-iwpiti2h 18 4 not not RB cord-103868-iwpiti2h 18 5 required require VBN cord-103868-iwpiti2h 18 6 for for IN cord-103868-iwpiti2h 18 7 STAT1 stat1 NN cord-103868-iwpiti2h 18 8 antagonism antagonism NN cord-103868-iwpiti2h 18 9 , , , cord-103868-iwpiti2h 18 10 consistent consistent JJ cord-103868-iwpiti2h 18 11 with with IN cord-103868-iwpiti2h 18 12 competitive competitive JJ cord-103868-iwpiti2h 18 13 karyopherin karyopherin NN cord-103868-iwpiti2h 18 14 binding bind VBG cord-103868-iwpiti2h 18 15 being be VBG cord-103868-iwpiti2h 18 16 the the DT cord-103868-iwpiti2h 18 17 principal principal JJ cord-103868-iwpiti2h 18 18 antagonistic antagonistic JJ cord-103868-iwpiti2h 18 19 mechanism mechanism NN cord-103868-iwpiti2h 18 20 while while IN cord-103868-iwpiti2h 18 21 export export NN cord-103868-iwpiti2h 18 22 mediates mediate VBZ cord-103868-iwpiti2h 18 23 return return NN cord-103868-iwpiti2h 18 24 of of IN cord-103868-iwpiti2h 18 25 nuclear nuclear JJ cord-103868-iwpiti2h 18 26 VP24 VP24 NNP cord-103868-iwpiti2h 18 27 to to IN cord-103868-iwpiti2h 18 28 the the DT cord-103868-iwpiti2h 18 29 cytoplasm cytoplasm NN cord-103868-iwpiti2h 18 30 for for IN cord-103868-iwpiti2h 18 31 replication replication NN cord-103868-iwpiti2h 18 32 functions function NNS cord-103868-iwpiti2h 18 33 . . . cord-103868-iwpiti2h 19 1 Thus thus RB cord-103868-iwpiti2h 19 2 , , , cord-103868-iwpiti2h 19 3 nuclear nuclear JJ cord-103868-iwpiti2h 19 4 export export NN cord-103868-iwpiti2h 19 5 of of IN cord-103868-iwpiti2h 19 6 VP24 VP24 NNP cord-103868-iwpiti2h 19 7 might may MD cord-103868-iwpiti2h 19 8 provide provide VB cord-103868-iwpiti2h 19 9 novel novel JJ cord-103868-iwpiti2h 19 10 targets target NNS cord-103868-iwpiti2h 19 11 for for IN cord-103868-iwpiti2h 19 12 antiviral antiviral JJ cord-103868-iwpiti2h 19 13 approaches approach NNS cord-103868-iwpiti2h 19 14 . . . cord-103868-iwpiti2h 20 1 Importance Importance NNP cord-103868-iwpiti2h 20 2 Ebola Ebola NNP cord-103868-iwpiti2h 20 3 virus virus NN cord-103868-iwpiti2h 20 4 ( ( -LRB- cord-103868-iwpiti2h 20 5 EBOV EBOV NNP cord-103868-iwpiti2h 20 6 ) ) -RRB- cord-103868-iwpiti2h 20 7 is be VBZ cord-103868-iwpiti2h 20 8 the the DT cord-103868-iwpiti2h 20 9 causative causative JJ cord-103868-iwpiti2h 20 10 agent agent NN cord-103868-iwpiti2h 20 11 of of IN cord-103868-iwpiti2h 20 12 ongoing ongoing JJ cord-103868-iwpiti2h 20 13 outbreaks outbreak NNS cord-103868-iwpiti2h 20 14 of of IN cord-103868-iwpiti2h 20 15 severe severe JJ cord-103868-iwpiti2h 20 16 haemorrhagic haemorrhagic JJ cord-103868-iwpiti2h 20 17 fever fever NN cord-103868-iwpiti2h 20 18 with with IN cord-103868-iwpiti2h 20 19 case case NN cord-103868-iwpiti2h 20 20 - - HYPH cord-103868-iwpiti2h 20 21 fatality fatality NN cord-103868-iwpiti2h 20 22 rates rate NNS cord-103868-iwpiti2h 20 23 between between IN cord-103868-iwpiti2h 20 24 40 40 CD cord-103868-iwpiti2h 20 25 and and CC cord-103868-iwpiti2h 20 26 60 60 CD cord-103868-iwpiti2h 20 27 % % NN cord-103868-iwpiti2h 20 28 . . . cord-103868-iwpiti2h 21 1 Proteins protein NNS cord-103868-iwpiti2h 21 2 of of IN cord-103868-iwpiti2h 21 3 many many JJ cord-103868-iwpiti2h 21 4 viruses virus NNS cord-103868-iwpiti2h 21 5 with with IN cord-103868-iwpiti2h 21 6 cytoplasmic cytoplasmic JJ cord-103868-iwpiti2h 21 7 replication replication NN cord-103868-iwpiti2h 21 8 cycles cycle NNS cord-103868-iwpiti2h 21 9 similar similar JJ cord-103868-iwpiti2h 21 10 to to IN cord-103868-iwpiti2h 21 11 EBOV EBOV NNP cord-103868-iwpiti2h 21 12 interact interact VB cord-103868-iwpiti2h 21 13 with with IN cord-103868-iwpiti2h 21 14 the the DT cord-103868-iwpiti2h 21 15 nuclear nuclear JJ cord-103868-iwpiti2h 21 16 trafficking trafficking NN cord-103868-iwpiti2h 21 17 machinery machinery NN cord-103868-iwpiti2h 21 18 , , , cord-103868-iwpiti2h 21 19 resulting result VBG cord-103868-iwpiti2h 21 20 in in IN cord-103868-iwpiti2h 21 21 active active JJ cord-103868-iwpiti2h 21 22 nucleocytoplasmic nucleocytoplasmic NN cord-103868-iwpiti2h 21 23 shuttling shuttle VBG cord-103868-iwpiti2h 21 24 important important JJ cord-103868-iwpiti2h 21 25 to to IN cord-103868-iwpiti2h 21 26 immune immune JJ cord-103868-iwpiti2h 21 27 evasion evasion NN cord-103868-iwpiti2h 21 28 and and CC cord-103868-iwpiti2h 21 29 other other JJ cord-103868-iwpiti2h 21 30 intranuclear intranuclear NN cord-103868-iwpiti2h 21 31 functions function NNS cord-103868-iwpiti2h 21 32 . . . cord-103868-iwpiti2h 22 1 However however RB cord-103868-iwpiti2h 22 2 , , , cord-103868-iwpiti2h 22 3 exploitation exploitation NN cord-103868-iwpiti2h 22 4 of of IN cord-103868-iwpiti2h 22 5 host host NN cord-103868-iwpiti2h 22 6 trafficking traffic VBG cord-103868-iwpiti2h 22 7 machinery machinery NN cord-103868-iwpiti2h 22 8 for for IN cord-103868-iwpiti2h 22 9 nucleocytoplasmic nucleocytoplasmic JJ cord-103868-iwpiti2h 22 10 transport transport NN cord-103868-iwpiti2h 22 11 by by IN cord-103868-iwpiti2h 22 12 EBOV EBOV NNP cord-103868-iwpiti2h 22 13 has have VBZ cord-103868-iwpiti2h 22 14 not not RB cord-103868-iwpiti2h 22 15 been be VBN cord-103868-iwpiti2h 22 16 directly directly RB cord-103868-iwpiti2h 22 17 examined examine VBN cord-103868-iwpiti2h 22 18 . . . cord-103868-iwpiti2h 23 1 We -PRON- PRP cord-103868-iwpiti2h 23 2 find find VBP cord-103868-iwpiti2h 23 3 that that IN cord-103868-iwpiti2h 23 4 the the DT cord-103868-iwpiti2h 23 5 EBOV EBOV NNP cord-103868-iwpiti2h 23 6 protein protein NN cord-103868-iwpiti2h 23 7 VP24 VP24 NNP cord-103868-iwpiti2h 23 8 is be VBZ cord-103868-iwpiti2h 23 9 actively actively RB cord-103868-iwpiti2h 23 10 trafficked traffic VBN cord-103868-iwpiti2h 23 11 between between IN cord-103868-iwpiti2h 23 12 the the DT cord-103868-iwpiti2h 23 13 nucleus nucleus NN cord-103868-iwpiti2h 23 14 and and CC cord-103868-iwpiti2h 23 15 cytoplasm cytoplasm JJ cord-103868-iwpiti2h 23 16 , , , cord-103868-iwpiti2h 23 17 and and CC cord-103868-iwpiti2h 23 18 identify identify VB cord-103868-iwpiti2h 23 19 the the DT cord-103868-iwpiti2h 23 20 specific specific JJ cord-103868-iwpiti2h 23 21 pathways pathway NNS cord-103868-iwpiti2h 23 22 and and CC cord-103868-iwpiti2h 23 23 sequences sequence NNS cord-103868-iwpiti2h 23 24 involved involve VBN cord-103868-iwpiti2h 23 25 . . . cord-103868-iwpiti2h 24 1 The the DT cord-103868-iwpiti2h 24 2 data datum NNS cord-103868-iwpiti2h 24 3 indicate indicate VBP cord-103868-iwpiti2h 24 4 that that IN cord-103868-iwpiti2h 24 5 nucleocytoplasmic nucleocytoplasmic NNP cord-103868-iwpiti2h 24 6 trafficking trafficking NN cord-103868-iwpiti2h 24 7 is be VBZ cord-103868-iwpiti2h 24 8 important important JJ cord-103868-iwpiti2h 24 9 for for IN cord-103868-iwpiti2h 24 10 the the DT cord-103868-iwpiti2h 24 11 multifunctional multifunctional JJ cord-103868-iwpiti2h 24 12 nature nature NN cord-103868-iwpiti2h 24 13 of of IN cord-103868-iwpiti2h 24 14 VP24 VP24 NNP cord-103868-iwpiti2h 24 15 , , , cord-103868-iwpiti2h 24 16 which which WDT cord-103868-iwpiti2h 24 17 has have VBZ cord-103868-iwpiti2h 24 18 critical critical JJ cord-103868-iwpiti2h 24 19 roles role NNS cord-103868-iwpiti2h 24 20 in in IN cord-103868-iwpiti2h 24 21 immune immune JJ cord-103868-iwpiti2h 24 22 evasion evasion NN cord-103868-iwpiti2h 24 23 and and CC cord-103868-iwpiti2h 24 24 viral viral JJ cord-103868-iwpiti2h 24 25 replication replication NN cord-103868-iwpiti2h 24 26 , , , cord-103868-iwpiti2h 24 27 identifying identify VBG cord-103868-iwpiti2h 24 28 a a DT cord-103868-iwpiti2h 24 29 new new JJ cord-103868-iwpiti2h 24 30 mechanism mechanism NN cord-103868-iwpiti2h 24 31 in in IN cord-103868-iwpiti2h 24 32 infection infection NN cord-103868-iwpiti2h 24 33 by by IN cord-103868-iwpiti2h 24 34 this this DT cord-103868-iwpiti2h 24 35 highly highly RB cord-103868-iwpiti2h 24 36 lethal lethal JJ cord-103868-iwpiti2h 24 37 pathogen pathogen NN cord-103868-iwpiti2h 24 38 , , , cord-103868-iwpiti2h 24 39 and and CC cord-103868-iwpiti2h 24 40 potential potential JJ cord-103868-iwpiti2h 24 41 target target NN cord-103868-iwpiti2h 24 42 for for IN cord-103868-iwpiti2h 24 43 antivirals antiviral NNS cord-103868-iwpiti2h 24 44 . . . cord-103868-iwpiti2h 25 1 transport transport NN cord-103868-iwpiti2h 25 2 , , , cord-103868-iwpiti2h 25 3 with with IN cord-103868-iwpiti2h 25 4 key key JJ cord-103868-iwpiti2h 25 5 roles role NNS cord-103868-iwpiti2h 25 6 in in IN cord-103868-iwpiti2h 25 7 pathogenesis pathogenesis NN cord-103868-iwpiti2h 25 8 . . . cord-103868-iwpiti2h 26 1 Despite despite IN cord-103868-iwpiti2h 26 2 established establish VBN cord-103868-iwpiti2h 26 3 karyopherin karyopherin NNP cord-103868-iwpiti2h 26 4 interaction interaction NN cord-103868-iwpiti2h 26 5 , , , cord-103868-iwpiti2h 26 6 the the DT cord-103868-iwpiti2h 26 7 23 23 CD cord-103868-iwpiti2h 26 8 nuclear nuclear JJ cord-103868-iwpiti2h 26 9 trafficking trafficking NN cord-103868-iwpiti2h 26 10 profile profile NN cord-103868-iwpiti2h 26 11 of of IN cord-103868-iwpiti2h 26 12 VP24 VP24 NNP cord-103868-iwpiti2h 26 13 has have VBZ cord-103868-iwpiti2h 26 14 not not RB cord-103868-iwpiti2h 26 15 been be VBN cord-103868-iwpiti2h 26 16 investigated investigate VBN cord-103868-iwpiti2h 26 17 . . . cord-103868-iwpiti2h 27 1 We -PRON- PRP cord-103868-iwpiti2h 27 2 find find VBP cord-103868-iwpiti2h 27 3 that that IN cord-103868-iwpiti2h 27 4 VP24 VP24 NNP cord-103868-iwpiti2h 27 5 becomes become VBZ cord-103868-iwpiti2h 27 6 24 24 CD cord-103868-iwpiti2h 27 7 strongly strongly RB cord-103868-iwpiti2h 27 8 nuclear nuclear JJ cord-103868-iwpiti2h 27 9 following follow VBG cord-103868-iwpiti2h 27 10 overexpression overexpression NN cord-103868-iwpiti2h 27 11 of of IN cord-103868-iwpiti2h 27 12 karyopherin karyopherin NN cord-103868-iwpiti2h 27 13 or or CC cord-103868-iwpiti2h 27 14 inhibition inhibition NN cord-103868-iwpiti2h 27 15 of of IN cord-103868-iwpiti2h 27 16 nuclear nuclear JJ cord-103868-iwpiti2h 27 17 export export NN cord-103868-iwpiti2h 27 18 25 25 CD cord-103868-iwpiti2h 27 19 pathways pathway NNS cord-103868-iwpiti2h 27 20 . . . cord-103868-iwpiti2h 28 1 Molecular molecular JJ cord-103868-iwpiti2h 28 2 mapping mapping NN cord-103868-iwpiti2h 28 3 indicates indicate VBZ cord-103868-iwpiti2h 28 4 that that IN cord-103868-iwpiti2h 28 5 cytoplasmic cytoplasmic JJ cord-103868-iwpiti2h 28 6 localisation localisation NN cord-103868-iwpiti2h 28 7 of of IN cord-103868-iwpiti2h 28 8 VP24 VP24 NNP cord-103868-iwpiti2h 28 9 depends depend VBZ cord-103868-iwpiti2h 28 10 on on IN cord-103868-iwpiti2h 28 11 a a DT cord-103868-iwpiti2h 28 12 26 26 CD cord-103868-iwpiti2h 28 13 CRM1-dependent CRM1-dependent NNP cord-103868-iwpiti2h 28 14 nuclear nuclear JJ cord-103868-iwpiti2h 28 15 export export NN cord-103868-iwpiti2h 28 16 sequence sequence NN cord-103868-iwpiti2h 28 17 at at IN cord-103868-iwpiti2h 28 18 the the DT cord-103868-iwpiti2h 28 19 VP24 VP24 NNP cord-103868-iwpiti2h 28 20 C C NNP cord-103868-iwpiti2h 28 21 - - HYPH cord-103868-iwpiti2h 28 22 terminus terminus NN cord-103868-iwpiti2h 28 23 . . . cord-103868-iwpiti2h 29 1 Nuclear nuclear JJ cord-103868-iwpiti2h 29 2 export export NN cord-103868-iwpiti2h 29 3 is be VBZ cord-103868-iwpiti2h 29 4 not not RB cord-103868-iwpiti2h 29 5 27 27 CD cord-103868-iwpiti2h 29 6 required require VBN cord-103868-iwpiti2h 29 7 for for IN cord-103868-iwpiti2h 29 8 STAT1 stat1 NN cord-103868-iwpiti2h 29 9 antagonism antagonism NN cord-103868-iwpiti2h 29 10 , , , cord-103868-iwpiti2h 29 11 consistent consistent JJ cord-103868-iwpiti2h 29 12 with with IN cord-103868-iwpiti2h 29 13 competitive competitive JJ cord-103868-iwpiti2h 29 14 karyopherin karyopherin NN cord-103868-iwpiti2h 29 15 binding bind VBG cord-103868-iwpiti2h 29 16 being be VBG cord-103868-iwpiti2h 29 17 the the DT cord-103868-iwpiti2h 29 18 28 28 CD cord-103868-iwpiti2h 29 19 principal principal JJ cord-103868-iwpiti2h 29 20 antagonistic antagonistic JJ cord-103868-iwpiti2h 29 21 mechanism mechanism NN cord-103868-iwpiti2h 29 22 while while IN cord-103868-iwpiti2h 29 23 export export NN cord-103868-iwpiti2h 29 24 mediates mediate VBZ cord-103868-iwpiti2h 29 25 return return NN cord-103868-iwpiti2h 29 26 of of IN cord-103868-iwpiti2h 29 27 nuclear nuclear JJ cord-103868-iwpiti2h 29 28 VP24 VP24 NNP cord-103868-iwpiti2h 29 29 to to IN cord-103868-iwpiti2h 29 30 the the DT cord-103868-iwpiti2h 29 31 29 29 CD cord-103868-iwpiti2h 29 32 cytoplasm cytoplasm NN cord-103868-iwpiti2h 29 33 for for IN cord-103868-iwpiti2h 29 34 replication replication NN cord-103868-iwpiti2h 29 35 functions function NNS cord-103868-iwpiti2h 29 36 . . . cord-103868-iwpiti2h 30 1 Thus thus RB cord-103868-iwpiti2h 30 2 , , , cord-103868-iwpiti2h 30 3 nuclear nuclear JJ cord-103868-iwpiti2h 30 4 export export NN cord-103868-iwpiti2h 30 5 of of IN cord-103868-iwpiti2h 30 6 VP24 VP24 NNP cord-103868-iwpiti2h 30 7 might may MD cord-103868-iwpiti2h 30 8 provide provide VB cord-103868-iwpiti2h 30 9 novel novel JJ cord-103868-iwpiti2h 30 10 targets target NNS cord-103868-iwpiti2h 30 11 30 30 CD cord-103868-iwpiti2h 30 12 for for IN cord-103868-iwpiti2h 30 13 antiviral antiviral JJ cord-103868-iwpiti2h 30 14 approaches approach NNS cord-103868-iwpiti2h 30 15 . . . cord-103868-iwpiti2h 31 1 HEK293 HEK293 NNP cord-103868-iwpiti2h 31 2 T t NN cord-103868-iwpiti2h 31 3 cells cell NNS cord-103868-iwpiti2h 31 4 ( ( -LRB- cord-103868-iwpiti2h 31 5 Figure Figure NNP cord-103868-iwpiti2h 31 6 S1 S1 NNP cord-103868-iwpiti2h 31 7 ) ) -RRB- cord-103868-iwpiti2h 31 8 . . . cord-103868-iwpiti2h 32 1 Thus thus RB cord-103868-iwpiti2h 32 2 , , , cord-103868-iwpiti2h 32 3 GFP GFP NNP cord-103868-iwpiti2h 32 4 - - HYPH cord-103868-iwpiti2h 32 5 VP24 VP24 NNP cord-103868-iwpiti2h 32 6 can can MD cord-103868-iwpiti2h 32 7 localise localise VB cord-103868-iwpiti2h 32 8 into into IN cord-103868-iwpiti2h 32 9 the the DT cord-103868-iwpiti2h 32 10 nucleus nucleus NN cord-103868-iwpiti2h 32 11 in in IN cord-103868-iwpiti2h 32 12 complexes complex NNS cord-103868-iwpiti2h 32 13 with with IN cord-103868-iwpiti2h 32 14 149 149 CD cord-103868-iwpiti2h 32 15 K1 K1 NNP cord-103868-iwpiti2h 32 16 , , , cord-103868-iwpiti2h 32 17 indicating indicate VBG cord-103868-iwpiti2h 32 18 that that IN cord-103868-iwpiti2h 32 19 cytoplasmic cytoplasmic JJ cord-103868-iwpiti2h 32 20 localisation localisation NN cord-103868-iwpiti2h 32 21 , , , cord-103868-iwpiti2h 32 22 which which WDT cord-103868-iwpiti2h 32 23 is be VBZ cord-103868-iwpiti2h 32 24 required require VBN cord-103868-iwpiti2h 32 25 for for IN cord-103868-iwpiti2h 32 26 roles role NNS cord-103868-iwpiti2h 32 27 in in IN cord-103868-iwpiti2h 32 28 nucleocapsid nucleocapsid NNP cord-103868-iwpiti2h 32 29 150 150 CD cord-103868-iwpiti2h 32 30 assembly assembly NN cord-103868-iwpiti2h 32 31 / / SYM cord-103868-iwpiti2h 32 32 condensation condensation NN cord-103868-iwpiti2h 32 33 ( ( -LRB- cord-103868-iwpiti2h 32 34 4 4 CD cord-103868-iwpiti2h 32 35 , , , cord-103868-iwpiti2h 32 36 5 5 CD cord-103868-iwpiti2h 32 37 , , , cord-103868-iwpiti2h 32 38 7 7 CD cord-103868-iwpiti2h 32 39 ) ) -RRB- cord-103868-iwpiti2h 32 40 , , , cord-103868-iwpiti2h 32 41 derives derive VBZ cord-103868-iwpiti2h 32 42 from from IN cord-103868-iwpiti2h 32 43 active active JJ cord-103868-iwpiti2h 32 44 nuclear nuclear JJ cord-103868-iwpiti2h 32 45 export export NN cord-103868-iwpiti2h 32 46 . . . cord-103868-iwpiti2h 33 1 can can MD cord-103868-iwpiti2h 33 2 diffuse diffuse VB cord-103868-iwpiti2h 33 3 through through IN cord-103868-iwpiti2h 33 4 the the DT cord-103868-iwpiti2h 33 5 NPC NPC NNP cord-103868-iwpiti2h 33 6 and and CC cord-103868-iwpiti2h 33 7 lacks lack VBZ cord-103868-iwpiti2h 33 8 NLSs nls NNS cord-103868-iwpiti2h 33 9 or or CC cord-103868-iwpiti2h 33 10 NESs ness NN cord-103868-iwpiti2h 33 11 , , , cord-103868-iwpiti2h 33 12 was be VBD cord-103868-iwpiti2h 33 13 diffusely diffusely RB cord-103868-iwpiti2h 33 14 localised localised JJ cord-103868-iwpiti2h 33 15 between between IN cord-103868-iwpiti2h 33 16 the the DT cord-103868-iwpiti2h 33 17 dependent dependent JJ cord-103868-iwpiti2h 33 18 NESs ness NN cord-103868-iwpiti2h 33 19 ( ( -LRB- cord-103868-iwpiti2h 33 20 Figure figure NN cord-103868-iwpiti2h 33 21 2A 2a CD cord-103868-iwpiti2h 33 22 , , , cord-103868-iwpiti2h 33 23 Figure figure NN cord-103868-iwpiti2h 33 24 S2A s2a NN cord-103868-iwpiti2h 33 25 ) ) -RRB- cord-103868-iwpiti2h 33 26 . . . cord-103868-iwpiti2h 34 1 Importantly importantly RB cord-103868-iwpiti2h 34 2 , , , cord-103868-iwpiti2h 34 3 the the DT cord-103868-iwpiti2h 34 4 Fn Fn NNP cord-103868-iwpiti2h 34 5 / / SYM cord-103868-iwpiti2h 34 6 c c NN cord-103868-iwpiti2h 34 7 for for IN cord-103868-iwpiti2h 34 8 GFP GFP NNP cord-103868-iwpiti2h 35 1 -VP241 -VP241 : cord-103868-iwpiti2h 35 2 - - SYM cord-103868-iwpiti2h 35 3 251 251 CD cord-103868-iwpiti2h 35 4 in in IN cord-103868-iwpiti2h 35 5 LMB-169 lmb-169 RB cord-103868-iwpiti2h 35 6 treated treat VBN cord-103868-iwpiti2h 35 7 cells cell NNS cord-103868-iwpiti2h 35 8 was be VBD cord-103868-iwpiti2h 35 9 higher high JJR cord-103868-iwpiti2h 35 10 than than IN cord-103868-iwpiti2h 35 11 that that DT cord-103868-iwpiti2h 35 12 for for IN cord-103868-iwpiti2h 35 13 GFP GFP NNP cord-103868-iwpiti2h 35 14 alone alone RB cord-103868-iwpiti2h 36 1 ( ( -LRB- cord-103868-iwpiti2h 36 2 Figure Figure NNP cord-103868-iwpiti2h 36 3 2C 2C NNP cord-103868-iwpiti2h 36 4 ) ) -RRB- cord-103868-iwpiti2h 36 5 , , , cord-103868-iwpiti2h 36 6 indicative indicative JJ cord-103868-iwpiti2h 36 7 of of IN cord-103868-iwpiti2h 36 8 accumulation accumulation NN cord-103868-iwpiti2h 36 9 . . . cord-103868-iwpiti2h 37 1 Thus thus RB cord-103868-iwpiti2h 37 2 , , , cord-103868-iwpiti2h 37 3 170 170 CD cord-103868-iwpiti2h 37 4 VP24 VP24 NNP cord-103868-iwpiti2h 37 5 localisation localisation NN cord-103868-iwpiti2h 37 6 appears appear VBZ cord-103868-iwpiti2h 37 7 to to TO cord-103868-iwpiti2h 37 8 be be VB cord-103868-iwpiti2h 37 9 dynamic dynamic JJ cord-103868-iwpiti2h 37 10 , , , cord-103868-iwpiti2h 37 11 involving involve VBG cord-103868-iwpiti2h 37 12 nuclear nuclear JJ cord-103868-iwpiti2h 37 13 entry entry NN cord-103868-iwpiti2h 37 14 and and CC cord-103868-iwpiti2h 37 15 rapid rapid JJ cord-103868-iwpiti2h 37 16 nuclear nuclear JJ cord-103868-iwpiti2h 37 17 export export NN cord-103868-iwpiti2h 37 18 via via IN cord-103868-iwpiti2h 37 19 171 171 CD cord-103868-iwpiti2h 37 20 CRM1 CRM1 NNP cord-103868-iwpiti2h 37 21 interaction interaction NN cord-103868-iwpiti2h 37 22 . . . cord-103868-iwpiti2h 38 1 172 172 CD cord-103868-iwpiti2h 39 1 To to TO cord-103868-iwpiti2h 39 2 determine determine VB cord-103868-iwpiti2h 39 3 which which WDT cord-103868-iwpiti2h 39 4 of of IN cord-103868-iwpiti2h 39 5 the the DT cord-103868-iwpiti2h 39 6 predicted predict VBN cord-103868-iwpiti2h 39 7 NESs ness NN cord-103868-iwpiti2h 39 8 is be VBZ cord-103868-iwpiti2h 39 9 / / , cord-103868-iwpiti2h 39 10 are be VBP cord-103868-iwpiti2h 39 11 responsible responsible JJ cord-103868-iwpiti2h 39 12 for for IN cord-103868-iwpiti2h 39 13 nuclear nuclear JJ cord-103868-iwpiti2h 39 14 export export NN cord-103868-iwpiti2h 39 15 , , , cord-103868-iwpiti2h 39 16 we -PRON- PRP cord-103868-iwpiti2h 39 17 generated generate VBD cord-103868-iwpiti2h 39 18 175 175 CD cord-103868-iwpiti2h 39 19 constructs construct NNS cord-103868-iwpiti2h 39 20 to to TO cord-103868-iwpiti2h 39 21 express express VB cord-103868-iwpiti2h 39 22 truncated truncated JJ cord-103868-iwpiti2h 39 23 VP24 VP24 NNP cord-103868-iwpiti2h 39 24 proteins protein NNS cord-103868-iwpiti2h 39 25 comprising comprise VBG cord-103868-iwpiti2h 39 26 N n NN cord-103868-iwpiti2h 39 27 - - HYPH cord-103868-iwpiti2h 39 28 terminal terminal NN cord-103868-iwpiti2h 39 29 ( ( -LRB- cord-103868-iwpiti2h 39 30 VP241 VP241 NNP cord-103868-iwpiti2h 39 31 - - HYPH cord-103868-iwpiti2h 39 32 88 88 CD cord-103868-iwpiti2h 39 33 ) ) -RRB- cord-103868-iwpiti2h 39 34 , , , cord-103868-iwpiti2h 39 35 central central JJ cord-103868-iwpiti2h 39 36 176 176 CD cord-103868-iwpiti2h 39 37 ( ( -LRB- cord-103868-iwpiti2h 39 38 VP2489 vp2489 NN cord-103868-iwpiti2h 39 39 - - HYPH cord-103868-iwpiti2h 39 40 172 172 CD cord-103868-iwpiti2h 39 41 ) ) -RRB- cord-103868-iwpiti2h 39 42 and and CC cord-103868-iwpiti2h 39 43 C c NN cord-103868-iwpiti2h 39 44 - - HYPH cord-103868-iwpiti2h 39 45 terminal terminal NN cord-103868-iwpiti2h 39 46 ( ( -LRB- cord-103868-iwpiti2h 39 47 VP24173 vp24173 NN cord-103868-iwpiti2h 39 48 - - HYPH cord-103868-iwpiti2h 39 49 251 251 CD cord-103868-iwpiti2h 39 50 ) ) -RRB- cord-103868-iwpiti2h 39 51 portions portion NNS cord-103868-iwpiti2h 39 52 fused fuse VBN cord-103868-iwpiti2h 39 53 to to IN cord-103868-iwpiti2h 39 54 GFP GFP NNP cord-103868-iwpiti2h 39 55 ; ; : cord-103868-iwpiti2h 39 56 each each DT cord-103868-iwpiti2h 39 57 of of IN cord-103868-iwpiti2h 39 58 these these DT cord-103868-iwpiti2h 39 59 contained contain VBN cord-103868-iwpiti2h 39 60 one one CD cord-103868-iwpiti2h 39 61 177 177 CD cord-103868-iwpiti2h 39 62 or or CC cord-103868-iwpiti2h 39 63 more more JJR cord-103868-iwpiti2h 39 64 of of IN cord-103868-iwpiti2h 39 65 the the DT cord-103868-iwpiti2h 39 66 potential potential JJ cord-103868-iwpiti2h 39 67 NESs ness NN cord-103868-iwpiti2h 39 68 ( ( -LRB- cord-103868-iwpiti2h 39 69 Figure figure NN cord-103868-iwpiti2h 39 70 2A 2a CD cord-103868-iwpiti2h 39 71 ) ) -RRB- cord-103868-iwpiti2h 39 72 . . . cord-103868-iwpiti2h 40 1 The the DT cord-103868-iwpiti2h 40 2 truncated truncated JJ cord-103868-iwpiti2h 40 3 proteins protein NNS cord-103868-iwpiti2h 40 4 were be VBD cord-103868-iwpiti2h 40 5 designed design VBN cord-103868-iwpiti2h 40 6 to to TO cord-103868-iwpiti2h 40 7 be be VB cord-103868-iwpiti2h 40 8 of of IN cord-103868-iwpiti2h 40 9 178 178 CD cord-103868-iwpiti2h 40 10 similar similar JJ cord-103868-iwpiti2h 40 11 length length NN cord-103868-iwpiti2h 40 12 and and CC cord-103868-iwpiti2h 40 13 to to TO cord-103868-iwpiti2h 40 14 avoid avoid VB cord-103868-iwpiti2h 40 15 disruption disruption NN cord-103868-iwpiti2h 40 16 of of IN cord-103868-iwpiti2h 40 17 key key JJ cord-103868-iwpiti2h 40 18 structural structural JJ cord-103868-iwpiti2h 40 19 elements element NNS cord-103868-iwpiti2h 40 20 ( ( -LRB- cord-103868-iwpiti2h 40 21 e.g. e.g. IN cord-103868-iwpiti2h 40 22 alpha alpha NN cord-103868-iwpiti2h 40 23 helices helix NNS cord-103868-iwpiti2h 40 24 and and CC cord-103868-iwpiti2h 40 25 beta beta NN cord-103868-iwpiti2h 40 26 179 179 CD cord-103868-iwpiti2h 40 27 sheets sheet NNS cord-103868-iwpiti2h 40 28 ) ) -RRB- cord-103868-iwpiti2h 40 29 , , , cord-103868-iwpiti2h 40 30 based base VBN cord-103868-iwpiti2h 40 31 on on IN cord-103868-iwpiti2h 40 32 the the DT cord-103868-iwpiti2h 40 33 VP24 VP24 NNP cord-103868-iwpiti2h 40 34 crystal crystal NN cord-103868-iwpiti2h 40 35 structure structure NN cord-103868-iwpiti2h 40 36 ( ( -LRB- cord-103868-iwpiti2h 40 37 20 20 CD cord-103868-iwpiti2h 40 38 ) ) -RRB- cord-103868-iwpiti2h 40 39 . . . cord-103868-iwpiti2h 41 1 All all DT cord-103868-iwpiti2h 41 2 proteins protein NNS cord-103868-iwpiti2h 41 3 were be VBD cord-103868-iwpiti2h 41 4 predominantly predominantly RB cord-103868-iwpiti2h 41 5 cytoplasmic cytoplasmic JJ cord-103868-iwpiti2h 41 6 180 180 CD cord-103868-iwpiti2h 41 7 at at IN cord-103868-iwpiti2h 41 8 steady steady JJ cord-103868-iwpiti2h 41 9 state state NN cord-103868-iwpiti2h 41 10 ( ( -LRB- cord-103868-iwpiti2h 41 11 Figure figure NN cord-103868-iwpiti2h 41 12 2B 2b CD cord-103868-iwpiti2h 41 13 ) ) -RRB- cord-103868-iwpiti2h 41 14 . . . cord-103868-iwpiti2h 42 1 Localisation localisation NN cord-103868-iwpiti2h 42 2 of of IN cord-103868-iwpiti2h 42 3 the the DT cord-103868-iwpiti2h 42 4 N n CD cord-103868-iwpiti2h 42 5 - - HYPH cord-103868-iwpiti2h 42 6 terminal terminal NN cord-103868-iwpiti2h 42 7 fragment fragment NN cord-103868-iwpiti2h 42 8 was be VBD cord-103868-iwpiti2h 42 9 largely largely RB cord-103868-iwpiti2h 42 10 unaffected unaffected JJ cord-103868-iwpiti2h 42 11 by by IN cord-103868-iwpiti2h 42 12 181 181 CD cord-103868-iwpiti2h 42 13 LMB LMB NNP cord-103868-iwpiti2h 42 14 treatment treatment NN cord-103868-iwpiti2h 42 15 , , , cord-103868-iwpiti2h 42 16 and and CC cord-103868-iwpiti2h 42 17 LMB LMB NNP cord-103868-iwpiti2h 42 18 produced produce VBD cord-103868-iwpiti2h 42 19 only only RB cord-103868-iwpiti2h 42 20 a a DT cord-103868-iwpiti2h 42 21 small small JJ cord-103868-iwpiti2h 42 22 ( ( -LRB- cord-103868-iwpiti2h 42 23 ≤ ≤ NN cord-103868-iwpiti2h 42 24 1.4 1.4 CD cord-103868-iwpiti2h 42 25 fold fold JJ cord-103868-iwpiti2h 42 26 ) ) -RRB- cord-103868-iwpiti2h 42 27 increase increase VB cord-103868-iwpiti2h 42 28 for for IN cord-103868-iwpiti2h 42 29 the the DT cord-103868-iwpiti2h 42 30 Fn Fn NNP cord-103868-iwpiti2h 42 31 / / SYM cord-103868-iwpiti2h 42 32 c c NN cord-103868-iwpiti2h 42 33 of of IN cord-103868-iwpiti2h 42 34 the the DT cord-103868-iwpiti2h 42 35 central central JJ cord-103868-iwpiti2h 42 36 182 182 CD cord-103868-iwpiti2h 42 37 fragment fragment NN cord-103868-iwpiti2h 42 38 ( ( -LRB- cord-103868-iwpiti2h 42 39 Figure figure NN cord-103868-iwpiti2h 42 40 2B 2b CD cord-103868-iwpiti2h 42 41 , , , cord-103868-iwpiti2h 42 42 C C NNP cord-103868-iwpiti2h 42 43 ) ) -RRB- cord-103868-iwpiti2h 42 44 . . . cord-103868-iwpiti2h 43 1 In in IN cord-103868-iwpiti2h 43 2 contrast contrast NN cord-103868-iwpiti2h 43 3 , , , cord-103868-iwpiti2h 43 4 a a DT cord-103868-iwpiti2h 43 5 consistent consistent JJ cord-103868-iwpiti2h 43 6 and and CC cord-103868-iwpiti2h 43 7 substantial substantial JJ cord-103868-iwpiti2h 43 8 increase increase NN cord-103868-iwpiti2h 43 9 ( ( -LRB- cord-103868-iwpiti2h 43 10 > > NFP cord-103868-iwpiti2h 43 11 2 2 CD cord-103868-iwpiti2h 43 12 fold fold VB cord-103868-iwpiti2h 43 13 ) ) -RRB- cord-103868-iwpiti2h 43 14 in in IN cord-103868-iwpiti2h 43 15 the the DT cord-103868-iwpiti2h 43 16 Fn Fn NNP cord-103868-iwpiti2h 43 17 / / SYM cord-103868-iwpiti2h 43 18 c c NN cord-103868-iwpiti2h 43 19 183 183 CD cord-103868-iwpiti2h 43 20 for for IN cord-103868-iwpiti2h 43 21 the the DT cord-103868-iwpiti2h 43 22 C C NNP cord-103868-iwpiti2h 43 23 - - HYPH cord-103868-iwpiti2h 43 24 terminal terminal NN cord-103868-iwpiti2h 43 25 fragment fragment NN cord-103868-iwpiti2h 43 26 was be VBD cord-103868-iwpiti2h 43 27 observed observe VBN cord-103868-iwpiti2h 43 28 following follow VBG cord-103868-iwpiti2h 43 29 LMB LMB NNP cord-103868-iwpiti2h 43 30 treatment treatment NN cord-103868-iwpiti2h 43 31 . . . cord-103868-iwpiti2h 44 1 VP24173 vp24173 NN cord-103868-iwpiti2h 44 2 - - NNP cord-103868-iwpiti2h 44 3 251 251 NNP cord-103868-iwpiti2h 44 4 also also RB cord-103868-iwpiti2h 44 5 displayed display VBD cord-103868-iwpiti2h 44 6 184 184 CD cord-103868-iwpiti2h 44 7 a a DT cord-103868-iwpiti2h 44 8 consistently consistently RB cord-103868-iwpiti2h 44 9 reduced reduce VBN cord-103868-iwpiti2h 44 10 Fn Fn NNP cord-103868-iwpiti2h 44 11 / / SYM cord-103868-iwpiti2h 44 12 c c NN cord-103868-iwpiti2h 44 13 at at IN cord-103868-iwpiti2h 44 14 steady steady JJ cord-103868-iwpiti2h 44 15 state state NN cord-103868-iwpiti2h 44 16 compared compare VBN cord-103868-iwpiti2h 44 17 with with IN cord-103868-iwpiti2h 44 18 the the DT cord-103868-iwpiti2h 44 19 other other JJ cord-103868-iwpiti2h 44 20 truncated truncated JJ cord-103868-iwpiti2h 44 21 proteins protein NNS cord-103868-iwpiti2h 44 22 . . . cord-103868-iwpiti2h 45 1 Thus thus RB cord-103868-iwpiti2h 45 2 , , , cord-103868-iwpiti2h 45 3 it -PRON- PRP cord-103868-iwpiti2h 45 4 185 185 CD cord-103868-iwpiti2h 45 5 appeared appear VBD cord-103868-iwpiti2h 45 6 that that IN cord-103868-iwpiti2h 45 7 prominent prominent JJ cord-103868-iwpiti2h 45 8 discrete discrete JJ cord-103868-iwpiti2h 45 9 CRM1-dependent CRM1-dependent NNP cord-103868-iwpiti2h 45 10 NES NES NNP cord-103868-iwpiti2h 45 11 activity activity NN cord-103868-iwpiti2h 45 12 is be VBZ cord-103868-iwpiti2h 45 13 located locate VBN cord-103868-iwpiti2h 45 14 in in IN cord-103868-iwpiti2h 45 15 the the DT cord-103868-iwpiti2h 45 16 C C NNP cord-103868-iwpiti2h 45 17 - - HYPH cord-103868-iwpiti2h 45 18 terminal terminal NN cord-103868-iwpiti2h 45 19 186 186 CD cord-103868-iwpiti2h 45 20 region region NN cord-103868-iwpiti2h 45 21 of of IN cord-103868-iwpiti2h 45 22 VP24 VP24 NNP cord-103868-iwpiti2h 45 23 . . . cord-103868-iwpiti2h 46 1 187 187 CD cord-103868-iwpiti2h 46 2 9 9 CD cord-103868-iwpiti2h 47 1 Notably notably RB cord-103868-iwpiti2h 47 2 , , , cord-103868-iwpiti2h 47 3 only only RB cord-103868-iwpiti2h 47 4 full full JJ cord-103868-iwpiti2h 47 5 - - HYPH cord-103868-iwpiti2h 47 6 length length NN cord-103868-iwpiti2h 47 7 VP24 VP24 NNP cord-103868-iwpiti2h 47 8 displayed display VBD cord-103868-iwpiti2h 47 9 accumulation accumulation NN cord-103868-iwpiti2h 47 10 into into IN cord-103868-iwpiti2h 47 11 the the DT cord-103868-iwpiti2h 47 12 nucleus nucleus NN cord-103868-iwpiti2h 47 13 following follow VBG cord-103868-iwpiti2h 47 14 LMB LMB NNP cord-103868-iwpiti2h 47 15 189 189 CD cord-103868-iwpiti2h 47 16 treatment treatment NN cord-103868-iwpiti2h 47 17 , , , cord-103868-iwpiti2h 47 18 with with IN cord-103868-iwpiti2h 47 19 all all DT cord-103868-iwpiti2h 47 20 truncated truncate VBN cord-103868-iwpiti2h 47 21 proteins protein NNS cord-103868-iwpiti2h 47 22 remaining remain VBG cord-103868-iwpiti2h 47 23 significantly significantly RB cord-103868-iwpiti2h 47 24 less less RBR cord-103868-iwpiti2h 47 25 nuclear nuclear JJ cord-103868-iwpiti2h 47 26 than than IN cord-103868-iwpiti2h 47 27 GFP GFP NNP cord-103868-iwpiti2h 47 28 alone alone RB cord-103868-iwpiti2h 47 29 . . . cord-103868-iwpiti2h 48 1 This this DT cord-103868-iwpiti2h 48 2 190 190 CD cord-103868-iwpiti2h 48 3 suggests suggest VBZ cord-103868-iwpiti2h 48 4 that that IN cord-103868-iwpiti2h 48 5 the the DT cord-103868-iwpiti2h 48 6 full full JJ cord-103868-iwpiti2h 48 7 protein protein NN cord-103868-iwpiti2h 48 8 sequence sequence NN cord-103868-iwpiti2h 48 9 is be VBZ cord-103868-iwpiti2h 48 10 required require VBN cord-103868-iwpiti2h 48 11 for for IN cord-103868-iwpiti2h 48 12 efficient efficient JJ cord-103868-iwpiti2h 48 13 nuclear nuclear JJ cord-103868-iwpiti2h 48 14 accumulation accumulation NN cord-103868-iwpiti2h 48 15 , , , cord-103868-iwpiti2h 48 16 such such JJ cord-103868-iwpiti2h 48 17 that that IN cord-103868-iwpiti2h 48 18 191 191 CD cord-103868-iwpiti2h 48 19 truncations truncation NNS cord-103868-iwpiti2h 48 20 remove remove VBP cord-103868-iwpiti2h 48 21 key key JJ cord-103868-iwpiti2h 48 22 sequences sequence NNS cord-103868-iwpiti2h 48 23 or or CC cord-103868-iwpiti2h 48 24 otherwise otherwise RB cord-103868-iwpiti2h 48 25 impact impact VB cord-103868-iwpiti2h 48 26 conformation conformation NN cord-103868-iwpiti2h 48 27 to to TO cord-103868-iwpiti2h 48 28 affect affect VB cord-103868-iwpiti2h 48 29 important important JJ cord-103868-iwpiti2h 48 30 192 192 CD cord-103868-iwpiti2h 48 31 interactions interaction NNS cord-103868-iwpiti2h 48 32 . . . cord-103868-iwpiti2h 49 1 The the DT cord-103868-iwpiti2h 49 2 crystal crystal JJ cord-103868-iwpiti2h 49 3 structure structure NN cord-103868-iwpiti2h 49 4 of of IN cord-103868-iwpiti2h 49 5 VP24 VP24 NNP cord-103868-iwpiti2h 49 6 bound bind VBN cord-103868-iwpiti2h 49 7 to to IN cord-103868-iwpiti2h 49 8 K5 k5 NN cord-103868-iwpiti2h 49 9 indicates indicate VBZ cord-103868-iwpiti2h 49 10 that that IN cord-103868-iwpiti2h 49 11 three three CD cord-103868-iwpiti2h 49 12 regions region NNS cord-103868-iwpiti2h 49 13 contact contact VBP cord-103868-iwpiti2h 49 14 193 193 CD cord-103868-iwpiti2h 49 15 the the DT cord-103868-iwpiti2h 49 16 K K NNP cord-103868-iwpiti2h 49 17 ( ( -LRB- cord-103868-iwpiti2h 49 18 CL1 CL1 NNP cord-103868-iwpiti2h 49 19 and and CC cord-103868-iwpiti2h 49 20 CL2/3 CL2/3 NNP cord-103868-iwpiti2h 49 21 , , , cord-103868-iwpiti2h 49 22 separated separate VBN cord-103868-iwpiti2h 49 23 by by IN cord-103868-iwpiti2h 49 24 40 40 CD cord-103868-iwpiti2h 49 25 - - SYM cord-103868-iwpiti2h 49 26 60 60 CD cord-103868-iwpiti2h 49 27 residues residue NNS cord-103868-iwpiti2h 49 28 , , , cord-103868-iwpiti2h 49 29 Figure figure NN cord-103868-iwpiti2h 49 30 2A 2a NN cord-103868-iwpiti2h 49 31 induced induce VBN cord-103868-iwpiti2h 49 32 only only RB cord-103868-iwpiti2h 49 33 a a DT cord-103868-iwpiti2h 49 34 small small JJ cord-103868-iwpiti2h 49 35 increase increase NN cord-103868-iwpiti2h 49 36 in in IN cord-103868-iwpiti2h 49 37 Fn Fn NNP cord-103868-iwpiti2h 49 38 / / SYM cord-103868-iwpiti2h 49 39 c c NN cord-103868-iwpiti2h 49 40 for for IN cord-103868-iwpiti2h 49 41 GFP gfp NN cord-103868-iwpiti2h 49 42 - - HYPH cord-103868-iwpiti2h 49 43 UL44NLS ul44nls JJ cord-103868-iwpiti2h 49 44 - - HYPH cord-103868-iwpiti2h 49 45 VP2489 vp2489 NN cord-103868-iwpiti2h 49 46 - - HYPH cord-103868-iwpiti2h 49 47 172 172 CD cord-103868-iwpiti2h 49 48 ( ( -LRB- cord-103868-iwpiti2h 49 49 Figure figure NN cord-103868-iwpiti2h 49 50 3A 3a NN cord-103868-iwpiti2h 49 51 , , , cord-103868-iwpiti2h 49 52 B B NNP cord-103868-iwpiti2h 49 53 ) ) -RRB- cord-103868-iwpiti2h 49 54 , , , cord-103868-iwpiti2h 49 55 suggestive suggestive JJ cord-103868-iwpiti2h 49 56 209 209 CD cord-103868-iwpiti2h 49 57 of of IN cord-103868-iwpiti2h 49 58 cytoplasmic cytoplasmic JJ cord-103868-iwpiti2h 49 59 retention retention NN cord-103868-iwpiti2h 49 60 or or CC cord-103868-iwpiti2h 49 61 nuclear nuclear JJ cord-103868-iwpiti2h 49 62 export export NN cord-103868-iwpiti2h 49 63 mediated mediate VBN cord-103868-iwpiti2h 49 64 largely largely RB cord-103868-iwpiti2h 49 65 via via IN cord-103868-iwpiti2h 49 66 an an DT cord-103868-iwpiti2h 49 67 alternative alternative JJ cord-103868-iwpiti2h 49 68 mechanism mechanism NN cord-103868-iwpiti2h 49 69 to to IN cord-103868-iwpiti2h 49 70 210 210 CD cord-103868-iwpiti2h 49 71 CRM1-dependent CRM1-dependent NNP cord-103868-iwpiti2h 49 72 export export NN cord-103868-iwpiti2h 49 73 . . . cord-103868-iwpiti2h 50 1 However however RB cord-103868-iwpiti2h 50 2 , , , cord-103868-iwpiti2h 50 3 LMB LMB NNP cord-103868-iwpiti2h 50 4 induced induce VBD cord-103868-iwpiti2h 50 5 substantial substantial JJ cord-103868-iwpiti2h 50 6 nuclear nuclear JJ cord-103868-iwpiti2h 50 7 localisation localisation NN cord-103868-iwpiti2h 50 8 of of IN cord-103868-iwpiti2h 50 9 GFP-211 GFP-211 NNP cord-103868-iwpiti2h 50 10 UL44NLS ul44nl NNS cord-103868-iwpiti2h 50 11 - - HYPH cord-103868-iwpiti2h 50 12 VP24173 vp24173 NN cord-103868-iwpiti2h 50 13 - - HYPH cord-103868-iwpiti2h 50 14 251 251 NN cord-103868-iwpiti2h 50 15 ( ( -LRB- cord-103868-iwpiti2h 50 16 > > NFP cord-103868-iwpiti2h 50 17 4.6 4.6 CD cord-103868-iwpiti2h 50 18 fold fold VB cord-103868-iwpiti2h 50 19 increase increase NN cord-103868-iwpiti2h 50 20 in in IN cord-103868-iwpiti2h 50 21 Fn Fn NNP cord-103868-iwpiti2h 50 22 / / SYM cord-103868-iwpiti2h 50 23 c c NN cord-103868-iwpiti2h 50 24 ; ; : cord-103868-iwpiti2h 50 25 Figure figure NN cord-103868-iwpiti2h 50 26 3B 3b CD cord-103868-iwpiti2h 50 27 ) ) -RRB- cord-103868-iwpiti2h 50 28 that that WDT cord-103868-iwpiti2h 50 29 clearly clearly RB cord-103868-iwpiti2h 50 30 exceeded exceed VBD cord-103868-iwpiti2h 50 31 nuclear nuclear JJ cord-103868-iwpiti2h 50 32 212 212 CD cord-103868-iwpiti2h 50 33 localisation localisation NN cord-103868-iwpiti2h 50 34 of of IN cord-103868-iwpiti2h 50 35 GFP GFP NNP cord-103868-iwpiti2h 50 36 - - HYPH cord-103868-iwpiti2h 50 37 VP24173 vp24173 NN cord-103868-iwpiti2h 50 38 - - HYPH cord-103868-iwpiti2h 50 39 251 251 NNP cord-103868-iwpiti2h 50 40 ( ( -LRB- cord-103868-iwpiti2h 50 41 Figure figure NN cord-103868-iwpiti2h 50 42 2C 2c NN cord-103868-iwpiti2h 50 43 ) ) -RRB- cord-103868-iwpiti2h 50 44 , , , cord-103868-iwpiti2h 50 45 consistent consistent JJ cord-103868-iwpiti2h 50 46 with with IN cord-103868-iwpiti2h 50 47 a a DT cord-103868-iwpiti2h 50 48 classical classical JJ cord-103868-iwpiti2h 50 49 CRM1-dependent CRM1-dependent NNP cord-103868-iwpiti2h 50 50 NES NES NNP cord-103868-iwpiti2h 50 51 213 213 CD cord-103868-iwpiti2h 50 52 counteracting counteract VBG cord-103868-iwpiti2h 50 53 the the DT cord-103868-iwpiti2h 50 54 activity activity NN cord-103868-iwpiti2h 50 55 of of IN cord-103868-iwpiti2h 50 56 the the DT cord-103868-iwpiti2h 50 57 heterologous heterologous JJ cord-103868-iwpiti2h 50 58 UL44 UL44 NNP cord-103868-iwpiti2h 50 59 NLS NLS NNP cord-103868-iwpiti2h 50 60 . . . cord-103868-iwpiti2h 51 1 The the DT cord-103868-iwpiti2h 51 2 Fn Fn NNP cord-103868-iwpiti2h 51 3 / / SYM cord-103868-iwpiti2h 51 4 c c NN cord-103868-iwpiti2h 51 5 for for IN cord-103868-iwpiti2h 51 6 GFP GFP NNP cord-103868-iwpiti2h 51 7 - - HYPH cord-103868-iwpiti2h 51 8 VP2489 VP2489 NNP cord-103868-iwpiti2h 51 9 - - HYPH cord-103868-iwpiti2h 51 10 251 251 CD cord-103868-iwpiti2h 51 11 was be VBD cord-103868-iwpiti2h 51 12 214 214 CD cord-103868-iwpiti2h 51 13 also also RB cord-103868-iwpiti2h 51 14 markedly markedly RB cord-103868-iwpiti2h 51 15 increased increase VBN cord-103868-iwpiti2h 51 16 by by IN cord-103868-iwpiti2h 51 17 LMB LMB NNP cord-103868-iwpiti2h 51 18 treatment treatment NN cord-103868-iwpiti2h 51 19 but but CC cord-103868-iwpiti2h 51 20 did do VBD cord-103868-iwpiti2h 51 21 not not RB cord-103868-iwpiti2h 51 22 attain attain VB cord-103868-iwpiti2h 51 23 an an DT cord-103868-iwpiti2h 51 24 Fn Fn NNP cord-103868-iwpiti2h 51 25 / / SYM cord-103868-iwpiti2h 51 26 c c NN cord-103868-iwpiti2h 51 27 similar similar JJ cord-103868-iwpiti2h 51 28 to to IN cord-103868-iwpiti2h 51 29 that that DT cord-103868-iwpiti2h 51 30 of of IN cord-103868-iwpiti2h 51 31 full-215 full-215 NNP cord-103868-iwpiti2h 51 32 required require VBN cord-103868-iwpiti2h 51 33 for for IN cord-103868-iwpiti2h 51 34 efficient efficient JJ cord-103868-iwpiti2h 51 35 nuclear nuclear JJ cord-103868-iwpiti2h 51 36 localisation localisation NN cord-103868-iwpiti2h 51 37 . . . cord-103868-iwpiti2h 52 1 Nevertheless nevertheless RB cord-103868-iwpiti2h 52 2 , , , cord-103868-iwpiti2h 52 3 these these DT cord-103868-iwpiti2h 52 4 data datum NNS cord-103868-iwpiti2h 52 5 clearly clearly RB cord-103868-iwpiti2h 52 6 indicate indicate VBP cord-103868-iwpiti2h 52 7 that that IN cord-103868-iwpiti2h 52 8 VP24 VP24 NNP cord-103868-iwpiti2h 52 9 217 217 CD cord-103868-iwpiti2h 52 10 contains contain VBZ cord-103868-iwpiti2h 52 11 classical classical JJ cord-103868-iwpiti2h 52 12 CRM1-dependent CRM1-dependent NNP cord-103868-iwpiti2h 52 13 NES NES NNP cord-103868-iwpiti2h 52 14 activity activity NN cord-103868-iwpiti2h 52 15 and and CC cord-103868-iwpiti2h 52 16 that that IN cord-103868-iwpiti2h 52 17 the the DT cord-103868-iwpiti2h 52 18 principal principal JJ cord-103868-iwpiti2h 52 19 NES NES NNP cord-103868-iwpiti2h 52 20 is be VBZ cord-103868-iwpiti2h 52 21 within within IN cord-103868-iwpiti2h 52 22 VP24173 vp24173 NN cord-103868-iwpiti2h 52 23 - - HYPH cord-103868-iwpiti2h 52 24 218 218 CD cord-103868-iwpiti2h 52 25 251 251 CD cord-103868-iwpiti2h 52 26 . . . cord-103868-iwpiti2h 53 1 The the DT cord-103868-iwpiti2h 53 2 C c NN cord-103868-iwpiti2h 53 3 - - HYPH cord-103868-iwpiti2h 53 4 terminal terminal NN cord-103868-iwpiti2h 53 5 CRM1-dependent CRM1-dependent NNP cord-103868-iwpiti2h 53 6 NES NES NNP cord-103868-iwpiti2h 53 7 is be VBZ cord-103868-iwpiti2h 53 8 the the DT cord-103868-iwpiti2h 53 9 principal principal JJ cord-103868-iwpiti2h 53 10 sequence sequence NN cord-103868-iwpiti2h 53 11 mediating mediate VBG cord-103868-iwpiti2h 53 12 nuclear nuclear JJ cord-103868-iwpiti2h 53 13 export export NN cord-103868-iwpiti2h 53 14 of of IN cord-103868-iwpiti2h 53 15 221 221 CD cord-103868-iwpiti2h 53 16 VP24 VP24 NNP cord-103868-iwpiti2h 53 17 222 222 CD cord-103868-iwpiti2h 53 18 To to TO cord-103868-iwpiti2h 53 19 confirm confirm VB cord-103868-iwpiti2h 53 20 that that IN cord-103868-iwpiti2h 53 21 the the DT cord-103868-iwpiti2h 53 22 C C NNP cord-103868-iwpiti2h 53 23 - - HYPH cord-103868-iwpiti2h 53 24 terminal terminal NN cord-103868-iwpiti2h 53 25 NES NES NNP cord-103868-iwpiti2h 53 26 is be VBZ cord-103868-iwpiti2h 53 27 the the DT cord-103868-iwpiti2h 53 28 major major JJ cord-103868-iwpiti2h 53 29 sequence sequence NN cord-103868-iwpiti2h 53 30 driving drive VBG cord-103868-iwpiti2h 53 31 CRM1-dependent CRM1-dependent NNP cord-103868-iwpiti2h 53 32 export export NN cord-103868-iwpiti2h 53 33 of of IN cord-103868-iwpiti2h 53 34 223 223 CD cord-103868-iwpiti2h 53 35 VP24 VP24 NNP cord-103868-iwpiti2h 53 36 , , , cord-103868-iwpiti2h 53 37 we -PRON- PRP cord-103868-iwpiti2h 53 38 used use VBD cord-103868-iwpiti2h 53 39 site site NN cord-103868-iwpiti2h 53 40 - - HYPH cord-103868-iwpiti2h 53 41 directed direct VBN cord-103868-iwpiti2h 53 42 mutagenesis mutagenesis NN cord-103868-iwpiti2h 53 43 to to TO cord-103868-iwpiti2h 53 44 disable disable VB cord-103868-iwpiti2h 53 45 the the DT cord-103868-iwpiti2h 53 46 NES NES NNP cord-103868-iwpiti2h 53 47 motif motif NN cord-103868-iwpiti2h 53 48 . . . cord-103868-iwpiti2h 54 1 Analysis analysis NN cord-103868-iwpiti2h 54 2 of of IN cord-103868-iwpiti2h 54 3 the the DT cord-103868-iwpiti2h 54 4 VP24 VP24 NNP cord-103868-iwpiti2h 54 5 C-224 c-224 NN cord-103868-iwpiti2h 54 6 terminal terminal NN cord-103868-iwpiti2h 54 7 region region NN cord-103868-iwpiti2h 54 8 identified identify VBD cord-103868-iwpiti2h 54 9 residues residue NNS cord-103868-iwpiti2h 54 10 241 241 CD cord-103868-iwpiti2h 54 11 - - HYPH cord-103868-iwpiti2h 54 12 251 251 CD cord-103868-iwpiti2h 54 13 ( ( -LRB- cord-103868-iwpiti2h 54 14 comprising comprise VBG cord-103868-iwpiti2h 54 15 the the DT cord-103868-iwpiti2h 54 16 C C NNP cord-103868-iwpiti2h 54 17 - - HYPH cord-103868-iwpiti2h 54 18 terminal terminal NN cord-103868-iwpiti2h 54 19 11 11 CD cord-103868-iwpiti2h 54 20 residues residue NNS cord-103868-iwpiti2h 54 21 ) ) -RRB- cord-103868-iwpiti2h 54 22 as as IN cord-103868-iwpiti2h 54 23 225 225 CD cord-103868-iwpiti2h 54 24 containing contain VBG cord-103868-iwpiti2h 54 25 a a DT cord-103868-iwpiti2h 54 26 sequence sequence NN cord-103868-iwpiti2h 54 27 strongly strongly RB cord-103868-iwpiti2h 54 28 conforming conform VBG cord-103868-iwpiti2h 54 29 to to IN cord-103868-iwpiti2h 54 30 a a DT cord-103868-iwpiti2h 54 31 NES nes NN cord-103868-iwpiti2h 54 32 ( ( -LRB- cord-103868-iwpiti2h 54 33 To to TO cord-103868-iwpiti2h 54 34 further further RB cord-103868-iwpiti2h 54 35 examine examine VB cord-103868-iwpiti2h 54 36 effects effect NNS cord-103868-iwpiti2h 54 37 of of IN cord-103868-iwpiti2h 54 38 altered altered JJ cord-103868-iwpiti2h 54 39 VP24 VP24 NNP cord-103868-iwpiti2h 54 40 nuclear nuclear JJ cord-103868-iwpiti2h 54 41 trafficking trafficking NN cord-103868-iwpiti2h 54 42 on on IN cord-103868-iwpiti2h 54 43 STAT1 stat1 NN cord-103868-iwpiti2h 54 44 responses response NNS cord-103868-iwpiti2h 54 45 , , , cord-103868-iwpiti2h 54 46 we -PRON- PRP cord-103868-iwpiti2h 54 47 263 263 CD cord-103868-iwpiti2h 54 48 assessed assess VBD cord-103868-iwpiti2h 54 49 nuclear nuclear JJ cord-103868-iwpiti2h 54 50 import import NN cord-103868-iwpiti2h 54 51 of of IN cord-103868-iwpiti2h 54 52 STAT1 stat1 NN cord-103868-iwpiti2h 54 53 using use VBG cord-103868-iwpiti2h 54 54 CLSM CLSM NNP cord-103868-iwpiti2h 54 55 analysis analysis NN cord-103868-iwpiti2h 54 56 of of IN cord-103868-iwpiti2h 54 57 COS7 COS7 NNP cord-103868-iwpiti2h 54 58 cells cell NNS cord-103868-iwpiti2h 54 59 expressing express VBG cord-103868-iwpiti2h 54 60 GFP GFP NNP cord-103868-iwpiti2h 54 61 - - HYPH cord-103868-iwpiti2h 54 62 VP24 VP24 NNP cord-103868-iwpiti2h 54 63 264 264 CD cord-103868-iwpiti2h 54 64 and and CC cord-103868-iwpiti2h 54 65 immunostained immunostaine VBN cord-103868-iwpiti2h 54 66 for for IN cord-103868-iwpiti2h 54 67 STAT1 stat1 NN cord-103868-iwpiti2h 54 68 following follow VBG cord-103868-iwpiti2h 54 69 treatment treatment NN cord-103868-iwpiti2h 54 70 without without IN cord-103868-iwpiti2h 54 71 or or CC cord-103868-iwpiti2h 54 72 with with IN cord-103868-iwpiti2h 54 73 IFN- IFN- NNP cord-103868-iwpiti2h 54 74 and/or and/or CC cord-103868-iwpiti2h 54 75 LMB LMB NNP cord-103868-iwpiti2h 54 76 . . . cord-103868-iwpiti2h 55 1 In in IN cord-103868-iwpiti2h 55 2 265 265 CD cord-103868-iwpiti2h 55 3 agreement agreement NN cord-103868-iwpiti2h 55 4 with with IN cord-103868-iwpiti2h 55 5 results result NNS cord-103868-iwpiti2h 55 6 of of IN cord-103868-iwpiti2h 55 7 the the DT cord-103868-iwpiti2h 55 8 luciferase luciferase NN cord-103868-iwpiti2h 55 9 reporter reporter NN cord-103868-iwpiti2h 55 10 assays assay NNS cord-103868-iwpiti2h 55 11 , , , cord-103868-iwpiti2h 55 12 we -PRON- PRP cord-103868-iwpiti2h 55 13 observed observe VBD cord-103868-iwpiti2h 55 14 that that IN cord-103868-iwpiti2h 55 15 despite despite IN cord-103868-iwpiti2h 55 16 substantial substantial JJ cord-103868-iwpiti2h 55 17 266 266 CD cord-103868-iwpiti2h 55 18 re re NN cord-103868-iwpiti2h 55 19 - - NN cord-103868-iwpiti2h 55 20 localisation localisation NN cord-103868-iwpiti2h 55 21 of of IN cord-103868-iwpiti2h 55 22 GFP GFP NNP cord-103868-iwpiti2h 55 23 - - HYPH cord-103868-iwpiti2h 55 24 VP24 VP24 NNP cord-103868-iwpiti2h 55 25 to to IN cord-103868-iwpiti2h 55 26 the the DT cord-103868-iwpiti2h 55 27 nucleus nucleus NN cord-103868-iwpiti2h 55 28 in in IN cord-103868-iwpiti2h 55 29 LMB LMB NNP cord-103868-iwpiti2h 55 30 - - HYPH cord-103868-iwpiti2h 55 31 treated treat VBN cord-103868-iwpiti2h 55 32 cells cell NNS cord-103868-iwpiti2h 55 33 , , , cord-103868-iwpiti2h 55 34 IFN--dependent ifn--dependent XX cord-103868-iwpiti2h 55 35 STAT1 stat1 NN cord-103868-iwpiti2h 55 36 267 267 CD cord-103868-iwpiti2h 55 37 nuclear nuclear JJ cord-103868-iwpiti2h 55 38 localisation localisation NN cord-103868-iwpiti2h 55 39 remained remain VBD cord-103868-iwpiti2h 55 40 clearly clearly RB cord-103868-iwpiti2h 55 41 inhibited inhibit VBN cord-103868-iwpiti2h 55 42 ( ( -LRB- cord-103868-iwpiti2h 55 43 Figure figure NN cord-103868-iwpiti2h 55 44 S3 S3 NNP cord-103868-iwpiti2h 55 45 ) ) -RRB- cord-103868-iwpiti2h 55 46 . . . cord-103868-iwpiti2h 56 1 Together together RB cord-103868-iwpiti2h 56 2 , , , cord-103868-iwpiti2h 56 3 these these DT cord-103868-iwpiti2h 56 4 data datum NNS cord-103868-iwpiti2h 56 5 indicate indicate VBP cord-103868-iwpiti2h 56 6 that that IN cord-103868-iwpiti2h 56 7 268 268 CD cord-103868-iwpiti2h 56 8 nuclear nuclear JJ cord-103868-iwpiti2h 56 9 export export NN cord-103868-iwpiti2h 56 10 of of IN cord-103868-iwpiti2h 56 11 VP24 VP24 NNP cord-103868-iwpiti2h 56 12 is be VBZ cord-103868-iwpiti2h 56 13 not not RB cord-103868-iwpiti2h 56 14 required require VBN cord-103868-iwpiti2h 56 15 for for IN cord-103868-iwpiti2h 56 16 inhibition inhibition NN cord-103868-iwpiti2h 56 17 of of IN cord-103868-iwpiti2h 56 18 STAT1 stat1 NN cord-103868-iwpiti2h 56 19 responses response NNS cord-103868-iwpiti2h 56 20 , , , cord-103868-iwpiti2h 56 21 consistent consistent JJ cord-103868-iwpiti2h 56 22 with with IN cord-103868-iwpiti2h 56 23 K K NNP cord-103868-iwpiti2h 56 24 269 269 CD cord-103868-iwpiti2h 56 25 binding bind VBG cord-103868-iwpiti2h 56 26 representing represent VBG cord-103868-iwpiti2h 56 27 the the DT cord-103868-iwpiti2h 56 28 major major JJ cord-103868-iwpiti2h 56 29 antagonistic antagonistic JJ cord-103868-iwpiti2h 56 30 mechanism mechanism NN cord-103868-iwpiti2h 56 31 . . . cord-103868-iwpiti2h 57 1 Thus thus RB cord-103868-iwpiti2h 57 2 , , , cord-103868-iwpiti2h 57 3 it -PRON- PRP cord-103868-iwpiti2h 57 4 appears appear VBZ cord-103868-iwpiti2h 57 5 that that IN cord-103868-iwpiti2h 57 6 active active JJ cord-103868-iwpiti2h 57 7 In in IN cord-103868-iwpiti2h 57 8 this this DT cord-103868-iwpiti2h 57 9 study study NN cord-103868-iwpiti2h 57 10 we -PRON- PRP cord-103868-iwpiti2h 57 11 have have VBP cord-103868-iwpiti2h 57 12 shown show VBN cord-103868-iwpiti2h 57 13 that that IN cord-103868-iwpiti2h 57 14 EBOV EBOV NNP cord-103868-iwpiti2h 57 15 VP24 VP24 NNP cord-103868-iwpiti2h 57 16 undergoes undergo VBZ cord-103868-iwpiti2h 57 17 active active JJ cord-103868-iwpiti2h 57 18 trafficking trafficking NN cord-103868-iwpiti2h 57 19 between between IN cord-103868-iwpiti2h 57 20 the the DT cord-103868-iwpiti2h 57 21 nucleus nucleus NN cord-103868-iwpiti2h 57 22 275 275 CD cord-103868-iwpiti2h 57 23 and and CC cord-103868-iwpiti2h 57 24 cytoplasm cytoplasm VBP cord-103868-iwpiti2h 57 25 involving involve VBG cord-103868-iwpiti2h 57 26 CRM1-dependent CRM1-dependent NNP cord-103868-iwpiti2h 57 27 nuclear nuclear JJ cord-103868-iwpiti2h 57 28 export export NN cord-103868-iwpiti2h 57 29 via via IN cord-103868-iwpiti2h 57 30 a a DT cord-103868-iwpiti2h 57 31 NES NES NNP cord-103868-iwpiti2h 57 32 at at IN cord-103868-iwpiti2h 57 33 the the DT cord-103868-iwpiti2h 57 34 VP24 VP24 NNP cord-103868-iwpiti2h 57 35 C C NNP cord-103868-iwpiti2h 57 36 - - HYPH cord-103868-iwpiti2h 57 37 terminus terminus NN cord-103868-iwpiti2h 57 38 . . . cord-103868-iwpiti2h 58 1 276 276 CD cord-103868-iwpiti2h 59 1 The the DT cord-103868-iwpiti2h 59 2 acquisition acquisition NN cord-103868-iwpiti2h 59 3 of of IN cord-103868-iwpiti2h 59 4 active active JJ cord-103868-iwpiti2h 59 5 nuclear nuclear JJ cord-103868-iwpiti2h 59 6 trafficking trafficking NN cord-103868-iwpiti2h 59 7 sequences sequence NNS cord-103868-iwpiti2h 59 8 is be VBZ cord-103868-iwpiti2h 59 9 consistent consistent JJ cord-103868-iwpiti2h 59 10 with with IN cord-103868-iwpiti2h 59 11 a a DT cord-103868-iwpiti2h 59 12 requirement requirement NN cord-103868-iwpiti2h 59 13 for for IN cord-103868-iwpiti2h 59 14 277 277 CD cord-103868-iwpiti2h 59 15 highly highly RB cord-103868-iwpiti2h 59 16 regulated regulated JJ cord-103868-iwpiti2h 59 17 / / SYM cord-103868-iwpiti2h 59 18 dynamic dynamic JJ cord-103868-iwpiti2h 59 19 localisation localisation NN cord-103868-iwpiti2h 59 20 ; ; : cord-103868-iwpiti2h 59 21 furthermore furthermore RB cord-103868-iwpiti2h 59 22 , , , cord-103868-iwpiti2h 59 23 since since IN cord-103868-iwpiti2h 59 24 VP24 VP24 NNP cord-103868-iwpiti2h 59 25 is be VBZ cord-103868-iwpiti2h 59 26 reported report VBN cord-103868-iwpiti2h 59 27 to to IN cord-103868-iwpiti2h 59 28 oligomerise oligomerise NNP cord-103868-iwpiti2h 59 29 278 278 CD cord-103868-iwpiti2h 59 30 ( ( -LRB- cord-103868-iwpiti2h 59 31 potentially potentially RB cord-103868-iwpiti2h 59 32 as as IN cord-103868-iwpiti2h 59 33 tetramers tetramer NNS cord-103868-iwpiti2h 59 34 ) ) -RRB- cord-103868-iwpiti2h 59 35 ( ( -LRB- cord-103868-iwpiti2h 59 36 38 38 CD cord-103868-iwpiti2h 59 37 ) ) -RRB- cord-103868-iwpiti2h 59 38 , , , cord-103868-iwpiti2h 59 39 it -PRON- PRP cord-103868-iwpiti2h 59 40 is be VBZ cord-103868-iwpiti2h 59 41 likely likely JJ cord-103868-iwpiti2h 59 42 that that IN cord-103868-iwpiti2h 59 43 active active JJ cord-103868-iwpiti2h 59 44 nuclear nuclear JJ cord-103868-iwpiti2h 59 45 trafficking trafficking NN cord-103868-iwpiti2h 59 46 is be VBZ cord-103868-iwpiti2h 59 47 required require VBN cord-103868-iwpiti2h 59 48 for for IN cord-103868-iwpiti2h 59 49 transport transport NN cord-103868-iwpiti2h 59 50 279 279 CD cord-103868-iwpiti2h 59 51 of of IN cord-103868-iwpiti2h 59 52 VP24 VP24 NNP cord-103868-iwpiti2h 59 53 multimers multimer NNS cord-103868-iwpiti2h 59 54 . . . cord-103868-iwpiti2h 60 1 The the DT cord-103868-iwpiti2h 60 2 identified identify VBN cord-103868-iwpiti2h 60 3 NES NES NNP cord-103868-iwpiti2h 60 4 was be VBD cord-103868-iwpiti2h 60 5 not not RB cord-103868-iwpiti2h 60 6 resolved resolve VBN cord-103868-iwpiti2h 60 7 in in IN cord-103868-iwpiti2h 60 8 VP24 VP24 NNP cord-103868-iwpiti2h 60 9 crystal crystal NN cord-103868-iwpiti2h 60 10 structures structure NNS cord-103868-iwpiti2h 60 11 ( ( -LRB- cord-103868-iwpiti2h 60 12 20 20 CD cord-103868-iwpiti2h 60 13 , , , cord-103868-iwpiti2h 60 14 47 47 CD cord-103868-iwpiti2h 60 15 , , , cord-103868-iwpiti2h 60 16 280 280 CD cord-103868-iwpiti2h 60 17 48 48 CD cord-103868-iwpiti2h 60 18 ) ) -RRB- cord-103868-iwpiti2h 60 19 but but CC cord-103868-iwpiti2h 60 20 localisation localisation NN cord-103868-iwpiti2h 60 21 at at IN cord-103868-iwpiti2h 60 22 the the DT cord-103868-iwpiti2h 60 23 C C NNP cord-103868-iwpiti2h 60 24 - - HYPH cord-103868-iwpiti2h 60 25 terminal terminal NN cord-103868-iwpiti2h 60 26 end end NN cord-103868-iwpiti2h 60 27 would would MD cord-103868-iwpiti2h 60 28 be be VB cord-103868-iwpiti2h 60 29 consistent consistent JJ cord-103868-iwpiti2h 60 30 with with IN cord-103868-iwpiti2h 60 31 exposure exposure NN cord-103868-iwpiti2h 60 32 and and CC cord-103868-iwpiti2h 60 33 accessibility accessibility NN cord-103868-iwpiti2h 60 34 281 281 CD cord-103868-iwpiti2h 60 35 to to IN cord-103868-iwpiti2h 60 36 CRM1 CRM1 NNP cord-103868-iwpiti2h 60 37 ( ( -LRB- cord-103868-iwpiti2h 60 38 20 20 CD cord-103868-iwpiti2h 60 39 ) ) -RRB- cord-103868-iwpiti2h 60 40 , , , cord-103868-iwpiti2h 60 41 and and CC cord-103868-iwpiti2h 60 42 the the DT cord-103868-iwpiti2h 60 43 predominantly predominantly RB cord-103868-iwpiti2h 60 44 cytoplasmic cytoplasmic JJ cord-103868-iwpiti2h 60 45 localisation localisation NN cord-103868-iwpiti2h 60 46 of of IN cord-103868-iwpiti2h 60 47 GFP GFP NNP cord-103868-iwpiti2h 60 48 - - HYPH cord-103868-iwpiti2h 60 49 VP24 VP24 NNP cord-103868-iwpiti2h 60 50 in in IN cord-103868-iwpiti2h 60 51 resting rest VBG cord-103868-iwpiti2h 60 52 cells cell NNS cord-103868-iwpiti2h 60 53 282 282 CD cord-103868-iwpiti2h 60 54 suggests suggest VBZ cord-103868-iwpiti2h 60 55 that that IN cord-103868-iwpiti2h 60 56 the the DT cord-103868-iwpiti2h 60 57 NES NES NNP cord-103868-iwpiti2h 60 58 is be VBZ cord-103868-iwpiti2h 60 59 the the DT cord-103868-iwpiti2h 60 60 dominant dominant JJ cord-103868-iwpiti2h 60 61 trafficking trafficking NN cord-103868-iwpiti2h 60 62 signal signal NN cord-103868-iwpiti2h 60 63 at at IN cord-103868-iwpiti2h 60 64 steady steady JJ cord-103868-iwpiti2h 60 65 state state NN cord-103868-iwpiti2h 60 66 . . . cord-103868-iwpiti2h 61 1 Intriguingly intriguingly RB cord-103868-iwpiti2h 61 2 , , , cord-103868-iwpiti2h 61 3 previous previous JJ cord-103868-iwpiti2h 61 4 283 283 CD cord-103868-iwpiti2h 61 5 studies study NNS cord-103868-iwpiti2h 61 6 indicated indicate VBD cord-103868-iwpiti2h 61 7 that that IN cord-103868-iwpiti2h 61 8 a a DT cord-103868-iwpiti2h 61 9 mutated mutated JJ cord-103868-iwpiti2h 61 10 VP24 VP24 NNP cord-103868-iwpiti2h 61 11 protein protein NN cord-103868-iwpiti2h 61 12 defective defective JJ cord-103868-iwpiti2h 61 13 for for IN cord-103868-iwpiti2h 61 14 K-binding k-binding NN cord-103868-iwpiti2h 61 15 was be VBD cord-103868-iwpiti2h 61 16 more more RBR cord-103868-iwpiti2h 61 17 cytoplasmic cytoplasmic JJ cord-103868-iwpiti2h 61 18 284 284 CD cord-103868-iwpiti2h 61 19 than than IN cord-103868-iwpiti2h 61 20 WT WT NNP cord-103868-iwpiti2h 61 21 protein protein NN cord-103868-iwpiti2h 61 22 ( ( -LRB- cord-103868-iwpiti2h 61 23 49 49 CD cord-103868-iwpiti2h 61 24 ) ) -RRB- cord-103868-iwpiti2h 61 25 . . . cord-103868-iwpiti2h 62 1 This this DT cord-103868-iwpiti2h 62 2 would would MD cord-103868-iwpiti2h 62 3 be be VB cord-103868-iwpiti2h 62 4 consistent consistent JJ cord-103868-iwpiti2h 62 5 with with IN cord-103868-iwpiti2h 62 6 karyopherin karyopherin NNP cord-103868-iwpiti2h 62 7 binding bind VBG cord-103868-iwpiti2h 62 8 mediating mediate VBG cord-103868-iwpiti2h 62 9 import import NN cord-103868-iwpiti2h 62 10 ; ; : cord-103868-iwpiti2h 62 11 285 285 CD cord-103868-iwpiti2h 62 12 one one CD cord-103868-iwpiti2h 62 13 might may MD cord-103868-iwpiti2h 62 14 thus thus RB cord-103868-iwpiti2h 62 15 speculate speculate VB cord-103868-iwpiti2h 62 16 that that IN cord-103868-iwpiti2h 62 17 VP24 VP24 NNP cord-103868-iwpiti2h 62 18 would would MD cord-103868-iwpiti2h 62 19 require require VB cord-103868-iwpiti2h 62 20 export export NN cord-103868-iwpiti2h 62 21 mechanisms mechanism NNS cord-103868-iwpiti2h 62 22 to to TO cord-103868-iwpiti2h 62 23 enable enable VB cord-103868-iwpiti2h 62 24 cytoplasmic cytoplasmic JJ cord-103868-iwpiti2h 62 25 286 286 CD cord-103868-iwpiti2h 62 26 localisation localisation NN cord-103868-iwpiti2h 62 27 / / SYM cord-103868-iwpiti2h 62 28 functions function NNS cord-103868-iwpiti2h 62 29 . . . cord-103868-iwpiti2h 63 1 Our -PRON- PRP$ cord-103868-iwpiti2h 63 2 findings finding NNS cord-103868-iwpiti2h 63 3 are be VBP cord-103868-iwpiti2h 63 4 the the DT cord-103868-iwpiti2h 63 5 first first JJ cord-103868-iwpiti2h 63 6 to to TO cord-103868-iwpiti2h 63 7 confirm confirm VB cord-103868-iwpiti2h 63 8 this this DT cord-103868-iwpiti2h 63 9 is be VBZ cord-103868-iwpiti2h 63 10 the the DT cord-103868-iwpiti2h 63 11 case case NN cord-103868-iwpiti2h 63 12 . . . cord-103868-iwpiti2h 64 1 Notably notably RB cord-103868-iwpiti2h 64 2 , , , cord-103868-iwpiti2h 64 3 the the DT cord-103868-iwpiti2h 64 4 EBOV EBOV NNP cord-103868-iwpiti2h 64 5 287 287 CD cord-103868-iwpiti2h 64 6 matrix matrix NN cord-103868-iwpiti2h 64 7 protein protein NN cord-103868-iwpiti2h 64 8 VP40 VP40 NNP cord-103868-iwpiti2h 64 9 has have VBZ cord-103868-iwpiti2h 64 10 also also RB cord-103868-iwpiti2h 64 11 been be VBN cord-103868-iwpiti2h 64 12 reported report VBN cord-103868-iwpiti2h 64 13 to to TO cord-103868-iwpiti2h 64 14 localise localise VB cord-103868-iwpiti2h 64 15 to to IN cord-103868-iwpiti2h 64 16 the the DT cord-103868-iwpiti2h 64 17 nucleus nucleus NN cord-103868-iwpiti2h 64 18 in in IN cord-103868-iwpiti2h 64 19 infected infected JJ cord-103868-iwpiti2h 64 20 and and CC cord-103868-iwpiti2h 64 21 transfected transfecte VBN cord-103868-iwpiti2h 64 22 288 288 CD cord-103868-iwpiti2h 64 23 cells cell NNS cord-103868-iwpiti2h 64 24 ( ( -LRB- cord-103868-iwpiti2h 64 25 16 16 CD cord-103868-iwpiti2h 64 26 , , , cord-103868-iwpiti2h 64 27 50 50 CD cord-103868-iwpiti2h 64 28 ) ) -RRB- cord-103868-iwpiti2h 64 29 ; ; : cord-103868-iwpiti2h 64 30 however however RB cord-103868-iwpiti2h 64 31 , , , cord-103868-iwpiti2h 64 32 a a DT cord-103868-iwpiti2h 64 33 direct direct JJ cord-103868-iwpiti2h 64 34 role role NN cord-103868-iwpiti2h 64 35 for for IN cord-103868-iwpiti2h 64 36 active active JJ cord-103868-iwpiti2h 64 37 trafficking traffic VBG cord-103868-iwpiti2h 64 38 pathways pathway NNS cord-103868-iwpiti2h 64 39 to to TO cord-103868-iwpiti2h 64 40 regulate regulate VB cord-103868-iwpiti2h 64 41 localisation localisation NN cord-103868-iwpiti2h 64 42 , , , cord-103868-iwpiti2h 64 43 289 289 CD cord-103868-iwpiti2h 64 44 distinct distinct JJ cord-103868-iwpiti2h 64 45 from from IN cord-103868-iwpiti2h 64 46 mechanisms mechanism NNS cord-103868-iwpiti2h 64 47 such such JJ cord-103868-iwpiti2h 64 48 as as IN cord-103868-iwpiti2h 64 49 diffusion diffusion NN cord-103868-iwpiti2h 64 50 or or CC cord-103868-iwpiti2h 64 51 interaction interaction NN cord-103868-iwpiti2h 64 52 with with IN cord-103868-iwpiti2h 64 53 other other JJ cord-103868-iwpiti2h 64 54 host host NN cord-103868-iwpiti2h 64 55 factors factor NNS cord-103868-iwpiti2h 64 56 , , , cord-103868-iwpiti2h 64 57 has have VBZ cord-103868-iwpiti2h 64 58 not not RB cord-103868-iwpiti2h 64 59 been be VBN cord-103868-iwpiti2h 64 60 290 290 CD cord-103868-iwpiti2h 64 61 defined define VBN cord-103868-iwpiti2h 64 62 . . . cord-103868-iwpiti2h 65 1 Thus thus RB cord-103868-iwpiti2h 65 2 , , , cord-103868-iwpiti2h 65 3 our -PRON- PRP$ cord-103868-iwpiti2h 65 4 data data NN cord-103868-iwpiti2h 65 5 provides provide VBZ cord-103868-iwpiti2h 65 6 , , , cord-103868-iwpiti2h 65 7 to to IN cord-103868-iwpiti2h 65 8 our -PRON- PRP$ cord-103868-iwpiti2h 65 9 knowledge knowledge NN cord-103868-iwpiti2h 65 10 , , , cord-103868-iwpiti2h 65 11 the the DT cord-103868-iwpiti2h 65 12 first first JJ cord-103868-iwpiti2h 65 13 direct direct JJ cord-103868-iwpiti2h 65 14 demonstration demonstration NN cord-103868-iwpiti2h 65 15 of of IN cord-103868-iwpiti2h 65 16 a a DT cord-103868-iwpiti2h 65 17 filovirus filovirus NN cord-103868-iwpiti2h 65 18 291 291 CD cord-103868-iwpiti2h 65 19 protein protein NN cord-103868-iwpiti2h 65 20 exploiting exploit VBG cord-103868-iwpiti2h 65 21 specific specific JJ cord-103868-iwpiti2h 65 22 host host NN cord-103868-iwpiti2h 65 23 trafficking traffic VBG cord-103868-iwpiti2h 65 24 machinery machinery NN cord-103868-iwpiti2h 65 25 for for IN cord-103868-iwpiti2h 65 26 nucleocytoplasmic nucleocytoplasmic NNP cord-103868-iwpiti2h 65 27 transport transport NN cord-103868-iwpiti2h 65 28 , , , cord-103868-iwpiti2h 65 29 292 292 CD cord-103868-iwpiti2h 65 30 identifying identify VBG cord-103868-iwpiti2h 65 31 a a DT cord-103868-iwpiti2h 65 32 new new JJ cord-103868-iwpiti2h 65 33 mechanism mechanism NN cord-103868-iwpiti2h 65 34 in in IN cord-103868-iwpiti2h 65 35 infection infection NN cord-103868-iwpiti2h 65 36 by by IN cord-103868-iwpiti2h 65 37 these these DT cord-103868-iwpiti2h 65 38 highly highly RB cord-103868-iwpiti2h 65 39 lethal lethal JJ cord-103868-iwpiti2h 65 40 pathogens pathogen NNS cord-103868-iwpiti2h 65 41 . . . cord-103868-iwpiti2h 66 1 293 293 CD cord-103868-iwpiti2h 66 2 294 294 CD cord-103868-iwpiti2h 67 1 Although although IN cord-103868-iwpiti2h 67 2 the the DT cord-103868-iwpiti2h 67 3 nucleus nucleus NN cord-103868-iwpiti2h 67 4 is be VBZ cord-103868-iwpiti2h 67 5 not not RB cord-103868-iwpiti2h 67 6 directly directly RB cord-103868-iwpiti2h 67 7 involved involve VBN cord-103868-iwpiti2h 67 8 in in IN cord-103868-iwpiti2h 67 9 the the DT cord-103868-iwpiti2h 67 10 replication replication NN cord-103868-iwpiti2h 67 11 processes process NNS cord-103868-iwpiti2h 67 12 of of IN cord-103868-iwpiti2h 67 13 most most JJS cord-103868-iwpiti2h 67 14 RNA RNA NNP cord-103868-iwpiti2h 67 15 viruses virus NNS cord-103868-iwpiti2h 67 16 , , , cord-103868-iwpiti2h 67 17 295 295 CD cord-103868-iwpiti2h 67 18 proteins protein NNS cord-103868-iwpiti2h 67 19 of of IN cord-103868-iwpiti2h 67 20 a a DT cord-103868-iwpiti2h 67 21 number number NN cord-103868-iwpiti2h 67 22 of of IN cord-103868-iwpiti2h 67 23 these these DT cord-103868-iwpiti2h 67 24 viruses virus NNS cord-103868-iwpiti2h 67 25 are be VBP cord-103868-iwpiti2h 67 26 reported report VBN cord-103868-iwpiti2h 67 27 to to TO cord-103868-iwpiti2h 67 28 encode encode VB cord-103868-iwpiti2h 67 29 nuclear nuclear JJ cord-103868-iwpiti2h 67 30 trafficking trafficking NN cord-103868-iwpiti2h 67 31 sequences sequence NNS cord-103868-iwpiti2h 67 32 , , , cord-103868-iwpiti2h 67 33 296 296 CD cord-103868-iwpiti2h 67 34 indicative indicative NN cord-103868-iwpiti2h 67 35 of of IN cord-103868-iwpiti2h 67 36 a a DT cord-103868-iwpiti2h 67 37 requirement requirement NN cord-103868-iwpiti2h 67 38 for for IN cord-103868-iwpiti2h 67 39 dynamic dynamic JJ cord-103868-iwpiti2h 67 40 regulation regulation NN cord-103868-iwpiti2h 67 41 or or CC cord-103868-iwpiti2h 67 42 specific specific JJ cord-103868-iwpiti2h 67 43 accumulation accumulation NN cord-103868-iwpiti2h 67 44 in in IN cord-103868-iwpiti2h 67 45 particular particular JJ cord-103868-iwpiti2h 67 46 297 297 CD cord-103868-iwpiti2h 67 47 compartments compartment NNS cord-103868-iwpiti2h 67 48 . . . cord-103868-iwpiti2h 68 1 For for IN cord-103868-iwpiti2h 68 2 example example NN cord-103868-iwpiti2h 68 3 , , , cord-103868-iwpiti2h 68 4 the the DT cord-103868-iwpiti2h 68 5 RABV RABV NNP cord-103868-iwpiti2h 68 6 IFN IFN NNP cord-103868-iwpiti2h 68 7 antagonist antagonist NN cord-103868-iwpiti2h 68 8 P p NN cord-103868-iwpiti2h 68 9 protein protein NN cord-103868-iwpiti2h 68 10 encodes encode VBZ cord-103868-iwpiti2h 68 11 several several JJ cord-103868-iwpiti2h 68 12 NLSs nls NNS cord-103868-iwpiti2h 68 13 and and CC cord-103868-iwpiti2h 68 14 298 298 CD cord-103868-iwpiti2h 68 15 NESs ness NN cord-103868-iwpiti2h 68 16 ( ( -LRB- cord-103868-iwpiti2h 68 17 32 32 CD cord-103868-iwpiti2h 68 18 , , , cord-103868-iwpiti2h 68 19 43 43 CD cord-103868-iwpiti2h 68 20 , , , cord-103868-iwpiti2h 68 21 51 51 CD cord-103868-iwpiti2h 68 22 - - SYM cord-103868-iwpiti2h 68 23 53 53 CD cord-103868-iwpiti2h 68 24 ) ) -RRB- cord-103868-iwpiti2h 68 25 , , , cord-103868-iwpiti2h 68 26 with with IN cord-103868-iwpiti2h 68 27 regulatory regulatory JJ cord-103868-iwpiti2h 68 28 mechanisms mechanism NNS cord-103868-iwpiti2h 68 29 including include VBG cord-103868-iwpiti2h 68 30 co co NN cord-103868-iwpiti2h 68 31 - - NN cord-103868-iwpiti2h 68 32 localisation localisation NN cord-103868-iwpiti2h 68 33 or or CC cord-103868-iwpiti2h 68 34 overlap overlap NN cord-103868-iwpiti2h 68 35 of of IN cord-103868-iwpiti2h 68 36 the the DT cord-103868-iwpiti2h 68 37 299 299 CD cord-103868-iwpiti2h 68 38 sequences sequence NNS cord-103868-iwpiti2h 68 39 , , , cord-103868-iwpiti2h 68 40 enabling enable VBG cord-103868-iwpiti2h 68 41 co co NN cord-103868-iwpiti2h 68 42 - - NN cord-103868-iwpiti2h 68 43 regulation regulation NN cord-103868-iwpiti2h 68 44 by by IN cord-103868-iwpiti2h 68 45 mechanisms mechanism NNS cord-103868-iwpiti2h 68 46 including include VBG cord-103868-iwpiti2h 68 47 phosphorylation phosphorylation NN cord-103868-iwpiti2h 68 48 ( ( -LRB- cord-103868-iwpiti2h 68 49 51 51 CD cord-103868-iwpiti2h 68 50 - - SYM cord-103868-iwpiti2h 68 51 53 53 CD cord-103868-iwpiti2h 68 52 ) ) -RRB- cord-103868-iwpiti2h 68 53 . . . cord-103868-iwpiti2h 69 1 300 300 CD cord-103868-iwpiti2h 70 1 Although although IN cord-103868-iwpiti2h 70 2 our -PRON- PRP$ cord-103868-iwpiti2h 70 3 data datum NNS cord-103868-iwpiti2h 70 4 identify identify VBP cord-103868-iwpiti2h 70 5 the the DT cord-103868-iwpiti2h 70 6 C C NNP cord-103868-iwpiti2h 70 7 - - HYPH cord-103868-iwpiti2h 70 8 terminal terminal NN cord-103868-iwpiti2h 70 9 NES NES NNP cord-103868-iwpiti2h 70 10 as as IN cord-103868-iwpiti2h 70 11 a a DT cord-103868-iwpiti2h 70 12 principal principal JJ cord-103868-iwpiti2h 70 13 determinant determinant NN cord-103868-iwpiti2h 70 14 of of IN cord-103868-iwpiti2h 70 15 nucleocytoplasmic nucleocytoplasmic NNP cord-103868-iwpiti2h 70 16 301 301 CD cord-103868-iwpiti2h 70 17 localisation localisation NN cord-103868-iwpiti2h 70 18 of of IN cord-103868-iwpiti2h 70 19 full full JJ cord-103868-iwpiti2h 70 20 - - HYPH cord-103868-iwpiti2h 70 21 length length NN cord-103868-iwpiti2h 70 22 VP24 VP24 NNP cord-103868-iwpiti2h 70 23 , , , cord-103868-iwpiti2h 70 24 the the DT cord-103868-iwpiti2h 70 25 differential differential JJ cord-103868-iwpiti2h 70 26 localisation localisation NN cord-103868-iwpiti2h 70 27 and and CC cord-103868-iwpiti2h 70 28 LMB LMB NNP cord-103868-iwpiti2h 70 29 sensitivity sensitivity NN cord-103868-iwpiti2h 70 30 of of IN cord-103868-iwpiti2h 70 31 VP241 VP241 NNP cord-103868-iwpiti2h 70 32 - - SYM cord-103868-iwpiti2h 70 33 88 88 CD cord-103868-iwpiti2h 70 34 302 302 CD cord-103868-iwpiti2h 70 35 and and CC cord-103868-iwpiti2h 70 36 VP2489 vp2489 NN cord-103868-iwpiti2h 70 37 - - HYPH cord-103868-iwpiti2h 70 38 172 172 CD cord-103868-iwpiti2h 70 39 , , , cord-103868-iwpiti2h 70 40 and and CC cord-103868-iwpiti2h 70 41 the the DT cord-103868-iwpiti2h 70 42 finding finding NN cord-103868-iwpiti2h 70 43 that that DT cord-103868-iwpiti2h 70 44 VP2489 vp2489 NN cord-103868-iwpiti2h 70 45 - - HYPH cord-103868-iwpiti2h 70 46 251 251 NN cord-103868-iwpiti2h 70 47 does do VBZ cord-103868-iwpiti2h 70 48 not not RB cord-103868-iwpiti2h 70 49 recapitulate recapitulate VB cord-103868-iwpiti2h 70 50 nuclear nuclear JJ cord-103868-iwpiti2h 70 51 accumulation accumulation NN cord-103868-iwpiti2h 70 52 of of IN cord-103868-iwpiti2h 70 53 303 303 CD cord-103868-iwpiti2h 70 54 full full JJ cord-103868-iwpiti2h 70 55 - - HYPH cord-103868-iwpiti2h 70 56 length length NN cord-103868-iwpiti2h 70 57 VP24 VP24 NNP cord-103868-iwpiti2h 70 58 , , , cord-103868-iwpiti2h 70 59 suggest suggest VBP cord-103868-iwpiti2h 70 60 the the DT cord-103868-iwpiti2h 70 61 presence presence NN cord-103868-iwpiti2h 70 62 of of IN cord-103868-iwpiti2h 70 63 alternative alternative JJ cord-103868-iwpiti2h 70 64 regulatory regulatory JJ cord-103868-iwpiti2h 70 65 sequences sequence NNS cord-103868-iwpiti2h 70 66 / / SYM cord-103868-iwpiti2h 70 67 mechanisms mechanism NNS cord-103868-iwpiti2h 70 68 , , , cord-103868-iwpiti2h 70 69 304 304 CD cord-103868-iwpiti2h 70 70 potentially potentially RB cord-103868-iwpiti2h 70 71 exposed expose VBN cord-103868-iwpiti2h 70 72 by by IN cord-103868-iwpiti2h 70 73 truncation truncation NN cord-103868-iwpiti2h 70 74 . . . cord-103868-iwpiti2h 71 1 For for IN cord-103868-iwpiti2h 71 2 example example NN cord-103868-iwpiti2h 71 3 , , , cord-103868-iwpiti2h 71 4 VP24 VP24 NNP cord-103868-iwpiti2h 71 5 is be VBZ cord-103868-iwpiti2h 71 6 reported report VBN cord-103868-iwpiti2h 71 7 to to TO cord-103868-iwpiti2h 71 8 associate associate VB cord-103868-iwpiti2h 71 9 with with IN cord-103868-iwpiti2h 71 10 membranes membrane NNS cord-103868-iwpiti2h 71 11 305 305 CD cord-103868-iwpiti2h 71 12 ( ( -LRB- cord-103868-iwpiti2h 71 13 38 38 CD cord-103868-iwpiti2h 71 14 ) ) -RRB- cord-103868-iwpiti2h 71 15 , , , cord-103868-iwpiti2h 71 16 which which WDT cord-103868-iwpiti2h 71 17 might may MD cord-103868-iwpiti2h 71 18 result result VB cord-103868-iwpiti2h 71 19 in in IN cord-103868-iwpiti2h 71 20 tethering tethering NN cord-103868-iwpiti2h 71 21 within within IN cord-103868-iwpiti2h 71 22 the the DT cord-103868-iwpiti2h 71 23 cytoplasm cytoplasm NN cord-103868-iwpiti2h 71 24 under under IN cord-103868-iwpiti2h 71 25 certain certain JJ cord-103868-iwpiti2h 71 26 conditions condition NNS cord-103868-iwpiti2h 71 27 . . . cord-103868-iwpiti2h 72 1 306 306 LS cord-103868-iwpiti2h 72 2 Interestingly interestingly RB cord-103868-iwpiti2h 72 3 , , , cord-103868-iwpiti2h 72 4 a a DT cord-103868-iwpiti2h 72 5 recent recent JJ cord-103868-iwpiti2h 72 6 study study NN cord-103868-iwpiti2h 72 7 reported report VBD cord-103868-iwpiti2h 72 8 that that IN cord-103868-iwpiti2h 72 9 sumoylation sumoylation NN cord-103868-iwpiti2h 72 10 of of IN cord-103868-iwpiti2h 72 11 residue residue NN cord-103868-iwpiti2h 72 12 K14 K14 NNP cord-103868-iwpiti2h 72 13 of of IN cord-103868-iwpiti2h 72 14 VP24 VP24 NNP cord-103868-iwpiti2h 72 15 enhances enhance VBZ cord-103868-iwpiti2h 72 16 K K NNP cord-103868-iwpiti2h 72 17 307 307 CD cord-103868-iwpiti2h 72 18 binding binding NN cord-103868-iwpiti2h 72 19 and and CC cord-103868-iwpiti2h 72 20 IFN IFN NNP cord-103868-iwpiti2h 72 21 antagonistic antagonistic JJ cord-103868-iwpiti2h 72 22 function function NN cord-103868-iwpiti2h 72 23 ( ( -LRB- cord-103868-iwpiti2h 72 24 54 54 CD cord-103868-iwpiti2h 72 25 ) ) -RRB- cord-103868-iwpiti2h 72 26 . . . cord-103868-iwpiti2h 73 1 In in IN cord-103868-iwpiti2h 73 2 contrast contrast NN cord-103868-iwpiti2h 73 3 , , , cord-103868-iwpiti2h 73 4 ubiquitination ubiquitination NN cord-103868-iwpiti2h 73 5 , , , cord-103868-iwpiti2h 73 6 including include VBG cord-103868-iwpiti2h 73 7 at at IN cord-103868-iwpiti2h 73 8 residue residue NN cord-103868-iwpiti2h 73 9 308 308 CD cord-103868-iwpiti2h 73 10 K206 K206 NNP cord-103868-iwpiti2h 73 11 within within IN cord-103868-iwpiti2h 73 12 CL3 CL3 NNP cord-103868-iwpiti2h 73 13 ( ( -LRB- cord-103868-iwpiti2h 73 14 Figure Figure NNP cord-103868-iwpiti2h 73 15 2A 2a CD cord-103868-iwpiti2h 73 16 ) ) -RRB- cord-103868-iwpiti2h 73 17 , , , cord-103868-iwpiti2h 73 18 appears appear VBZ cord-103868-iwpiti2h 73 19 to to TO cord-103868-iwpiti2h 73 20 negatively negatively RB cord-103868-iwpiti2h 73 21 regulate regulate VB cord-103868-iwpiti2h 73 22 IFN IFN NNP cord-103868-iwpiti2h 73 23 antagonist antagonist NN cord-103868-iwpiti2h 73 24 activity activity NN cord-103868-iwpiti2h 73 25 ( ( -LRB- cord-103868-iwpiti2h 73 26 54 54 CD cord-103868-iwpiti2h 73 27 ) ) -RRB- cord-103868-iwpiti2h 73 28 . . . cord-103868-iwpiti2h 74 1 309 309 CD cord-103868-iwpiti2h 75 1 Intriguingly intriguingly RB cord-103868-iwpiti2h 75 2 , , , cord-103868-iwpiti2h 75 3 K14 K14 NNP cord-103868-iwpiti2h 75 4 is be VBZ cord-103868-iwpiti2h 75 5 distal distal JJ cord-103868-iwpiti2h 75 6 to to IN cord-103868-iwpiti2h 75 7 CL1 CL1 NNP cord-103868-iwpiti2h 75 8 - - HYPH cord-103868-iwpiti2h 75 9 3 3 NNP cord-103868-iwpiti2h 75 10 but but CC cord-103868-iwpiti2h 75 11 is be VBZ cord-103868-iwpiti2h 75 12 within within IN cord-103868-iwpiti2h 75 13 a a DT cord-103868-iwpiti2h 75 14 predicted predict VBN cord-103868-iwpiti2h 75 15 NES NES NNP cord-103868-iwpiti2h 75 16 motif motif NN cord-103868-iwpiti2h 75 17 ( ( -LRB- cord-103868-iwpiti2h 75 18 Figure figure NN cord-103868-iwpiti2h 75 19 2A 2a CD cord-103868-iwpiti2h 75 20 ) ) -RRB- cord-103868-iwpiti2h 75 21 . . . cord-103868-iwpiti2h 76 1 Whether whether IN cord-103868-iwpiti2h 76 2 mechanisms mechanism NNS cord-103868-iwpiti2h 76 3 will will MD cord-103868-iwpiti2h 76 4 be be VB cord-103868-iwpiti2h 76 5 of of IN cord-103868-iwpiti2h 76 6 interest interest NN cord-103868-iwpiti2h 76 7 in in IN cord-103868-iwpiti2h 76 8 defining define VBG cord-103868-iwpiti2h 76 9 the the DT cord-103868-iwpiti2h 76 10 processes process NNS cord-103868-iwpiti2h 76 11 controlling control VBG cord-103868-iwpiti2h 76 12 immune immune JJ cord-103868-iwpiti2h 76 13 evasion evasion NN cord-103868-iwpiti2h 76 14 and and CC cord-103868-iwpiti2h 76 15 312 312 CD cord-103868-iwpiti2h 76 16 replication replication NN cord-103868-iwpiti2h 76 17 by by IN cord-103868-iwpiti2h 76 18 EBOV EBOV NNP cord-103868-iwpiti2h 76 19 . . . cord-103868-iwpiti2h 77 1 313 313 CD cord-103868-iwpiti2h 78 1 While while IN cord-103868-iwpiti2h 78 2 some some DT cord-103868-iwpiti2h 78 3 viral viral JJ cord-103868-iwpiti2h 78 4 IFN IFN NNP cord-103868-iwpiti2h 78 5 antagonists antagonist NNS cord-103868-iwpiti2h 78 6 use use VBP cord-103868-iwpiti2h 78 7 NESs ness NN cord-103868-iwpiti2h 78 8 to to TO cord-103868-iwpiti2h 78 9 facilitate facilitate VB cord-103868-iwpiti2h 78 10 immune immune JJ cord-103868-iwpiti2h 78 11 evasion evasion NN cord-103868-iwpiti2h 78 12 , , , cord-103868-iwpiti2h 78 13 including include VBG cord-103868-iwpiti2h 78 14 through through IN cord-103868-iwpiti2h 78 15 315 315 CD cord-103868-iwpiti2h 78 16 mislocalisation mislocalisation NN cord-103868-iwpiti2h 78 17 of of IN cord-103868-iwpiti2h 78 18 associated associate VBN cord-103868-iwpiti2h 78 19 STATs STATs NNP cord-103868-iwpiti2h 78 20 ( ( -LRB- cord-103868-iwpiti2h 78 21 33 33 CD cord-103868-iwpiti2h 78 22 , , , cord-103868-iwpiti2h 78 23 34 34 CD cord-103868-iwpiti2h 78 24 ) ) -RRB- cord-103868-iwpiti2h 78 25 , , , cord-103868-iwpiti2h 78 26 VP24 VP24 NNP cord-103868-iwpiti2h 78 27 uses use VBZ cord-103868-iwpiti2h 78 28 a a DT cord-103868-iwpiti2h 78 29 mechanism mechanism NN cord-103868-iwpiti2h 78 30 of of IN cord-103868-iwpiti2h 78 31 competitive competitive JJ cord-103868-iwpiti2h 78 32 binding bind VBG cord-103868-iwpiti2h 78 33 316 316 CD cord-103868-iwpiti2h 78 34 to to IN cord-103868-iwpiti2h 78 35 Ks ks NN cord-103868-iwpiti2h 78 36 . . . cord-103868-iwpiti2h 79 1 Our -PRON- PRP$ cord-103868-iwpiti2h 79 2 finding finding NN cord-103868-iwpiti2h 79 3 that that WDT cord-103868-iwpiti2h 79 4 VP24 VP24 NNP cord-103868-iwpiti2h 79 5 nuclear nuclear JJ cord-103868-iwpiti2h 79 6 export export NN cord-103868-iwpiti2h 79 7 is be VBZ cord-103868-iwpiti2h 79 8 not not RB cord-103868-iwpiti2h 79 9 required require VBN cord-103868-iwpiti2h 79 10 for for IN cord-103868-iwpiti2h 79 11 STAT stat NN cord-103868-iwpiti2h 79 12 antagonism antagonism NN cord-103868-iwpiti2h 79 13 is be VBZ cord-103868-iwpiti2h 79 14 consistent consistent JJ cord-103868-iwpiti2h 79 15 317 317 CD cord-103868-iwpiti2h 79 16 with with IN cord-103868-iwpiti2h 79 17 this this DT cord-103868-iwpiti2h 79 18 , , , cord-103868-iwpiti2h 79 19 and and CC cord-103868-iwpiti2h 79 20 indicates indicate VBZ cord-103868-iwpiti2h 79 21 that that IN cord-103868-iwpiti2h 79 22 export export NN cord-103868-iwpiti2h 79 23 relates relate VBZ cord-103868-iwpiti2h 79 24 to to IN cord-103868-iwpiti2h 79 25 cytoplasmic cytoplasmic JJ cord-103868-iwpiti2h 79 26 roles role NNS cord-103868-iwpiti2h 79 27 including include VBG cord-103868-iwpiti2h 79 28 in in IN cord-103868-iwpiti2h 79 29 nucleocapsid nucleocapsid NNP cord-103868-iwpiti2h 79 30 318 318 CD cord-103868-iwpiti2h 79 31 assembly assembly NN cord-103868-iwpiti2h 79 32 and and CC cord-103868-iwpiti2h 79 33 transport transport NN cord-103868-iwpiti2h 79 34 ( ( -LRB- cord-103868-iwpiti2h 79 35 4 4 CD cord-103868-iwpiti2h 79 36 - - SYM cord-103868-iwpiti2h 79 37 14 14 CD cord-103868-iwpiti2h 79 38 ) ) -RRB- cord-103868-iwpiti2h 79 39 . . . cord-103868-iwpiti2h 80 1 The the DT cord-103868-iwpiti2h 80 2 requirement requirement NN cord-103868-iwpiti2h 80 3 for for IN cord-103868-iwpiti2h 80 4 efficient efficient JJ cord-103868-iwpiti2h 80 5 translocation translocation NN cord-103868-iwpiti2h 80 6 out out IN cord-103868-iwpiti2h 80 7 of of IN cord-103868-iwpiti2h 80 8 the the DT cord-103868-iwpiti2h 80 9 nucleus nucleus NN cord-103868-iwpiti2h 80 10 is be VBZ cord-103868-iwpiti2h 80 11 319 319 CD cord-103868-iwpiti2h 80 12 consistent consistent JJ cord-103868-iwpiti2h 80 13 with with IN cord-103868-iwpiti2h 80 14 interaction interaction NN cord-103868-iwpiti2h 80 15 of of IN cord-103868-iwpiti2h 80 16 VP24 VP24 NNP cord-103868-iwpiti2h 80 17 with with IN cord-103868-iwpiti2h 80 18 K K NNP cord-103868-iwpiti2h 80 19 ( ( -LRB- cord-103868-iwpiti2h 80 20 see see VB cord-103868-iwpiti2h 80 21 above above RB cord-103868-iwpiti2h 80 22 ) ) -RRB- cord-103868-iwpiti2h 80 23 , , , cord-103868-iwpiti2h 80 24 that that WDT cord-103868-iwpiti2h 80 25 underpins underpin VBZ cord-103868-iwpiti2h 80 26 distinct distinct JJ cord-103868-iwpiti2h 80 27 functions function NNS cord-103868-iwpiti2h 80 28 in in IN cord-103868-iwpiti2h 80 29 320 320 CD cord-103868-iwpiti2h 80 30 immune immune JJ cord-103868-iwpiti2h 80 31 evasion evasion NN cord-103868-iwpiti2h 80 32 . . . cord-103868-iwpiti2h 81 1 This this DT cord-103868-iwpiti2h 81 2 is be VBZ cord-103868-iwpiti2h 81 3 further further RB cord-103868-iwpiti2h 81 4 supported support VBN cord-103868-iwpiti2h 81 5 by by IN cord-103868-iwpiti2h 81 6 our -PRON- PRP$ cord-103868-iwpiti2h 81 7 finding finding NN cord-103868-iwpiti2h 81 8 that that IN cord-103868-iwpiti2h 81 9 the the DT cord-103868-iwpiti2h 81 10 C C NNP cord-103868-iwpiti2h 81 11 - - HYPH cord-103868-iwpiti2h 81 12 terminal terminal NN cord-103868-iwpiti2h 81 13 NES NES NNP cord-103868-iwpiti2h 81 14 motif motif NN cord-103868-iwpiti2h 81 15 is be VBZ cord-103868-iwpiti2h 81 16 321 321 CD cord-103868-iwpiti2h 81 17 conserved conserve VBN cord-103868-iwpiti2h 81 18 among among IN cord-103868-iwpiti2h 81 19 VP24 VP24 NNP cord-103868-iwpiti2h 81 20 of of IN cord-103868-iwpiti2h 81 21 several several JJ cord-103868-iwpiti2h 81 22 filovirus filovirus NN cord-103868-iwpiti2h 81 23 species specie NNS cord-103868-iwpiti2h 81 24 that that WDT cord-103868-iwpiti2h 81 25 have have VBP cord-103868-iwpiti2h 81 26 been be VBN cord-103868-iwpiti2h 81 27 shown show VBN cord-103868-iwpiti2h 81 28 to to TO cord-103868-iwpiti2h 81 29 bind bind VB cord-103868-iwpiti2h 81 30 to to IN cord-103868-iwpiti2h 81 31 Ks ks NN cord-103868-iwpiti2h 81 32 or or CC cord-103868-iwpiti2h 81 33 322 322 CD cord-103868-iwpiti2h 81 34 have have VBP cord-103868-iwpiti2h 81 35 conserved conserve VBN cord-103868-iwpiti2h 81 36 CL CL NNP cord-103868-iwpiti2h 81 37 sequences sequence NNS cord-103868-iwpiti2h 81 38 ( ( -LRB- cord-103868-iwpiti2h 81 39 20 20 CD cord-103868-iwpiti2h 81 40 , , , cord-103868-iwpiti2h 81 41 26 26 CD cord-103868-iwpiti2h 81 42 , , , cord-103868-iwpiti2h 81 43 45 45 CD cord-103868-iwpiti2h 81 44 ) ) -RRB- cord-103868-iwpiti2h 81 45 , , , cord-103868-iwpiti2h 81 46 but but CC cord-103868-iwpiti2h 81 47 not not RB cord-103868-iwpiti2h 81 48 in in IN cord-103868-iwpiti2h 81 49 MABV MABV NNP cord-103868-iwpiti2h 81 50 VP24 VP24 NNP cord-103868-iwpiti2h 81 51 ( ( -LRB- cord-103868-iwpiti2h 81 52 Figure figure NN cord-103868-iwpiti2h 81 53 4E 4e NN cord-103868-iwpiti2h 81 54 , , . cord-103868-iwpiti2h 82 1 Figure figure NN cord-103868-iwpiti2h 82 2 S2C s2c CD cord-103868-iwpiti2h 83 1 Thus thus RB cord-103868-iwpiti2h 83 2 , , , cord-103868-iwpiti2h 83 3 targeting target VBG cord-103868-iwpiti2h 83 4 VP24 VP24 NNP cord-103868-iwpiti2h 83 5 regulatory regulatory JJ cord-103868-iwpiti2h 83 6 mechanisms mechanism NNS cord-103868-iwpiti2h 83 7 , , , cord-103868-iwpiti2h 83 8 including include VBG cord-103868-iwpiti2h 83 9 its -PRON- PRP$ cord-103868-iwpiti2h 83 10 nuclear nuclear JJ cord-103868-iwpiti2h 83 11 export export NN cord-103868-iwpiti2h 83 12 , , , cord-103868-iwpiti2h 83 13 may may MD cord-103868-iwpiti2h 83 14 provide provide VB cord-103868-iwpiti2h 83 15 novel novel JJ cord-103868-iwpiti2h 83 16 353 353 CD cord-103868-iwpiti2h 83 17 targets target NNS cord-103868-iwpiti2h 83 18 for for IN cord-103868-iwpiti2h 83 19 anti anti JJ cord-103868-iwpiti2h 83 20 - - JJ cord-103868-iwpiti2h 83 21 EBOV ebov JJ cord-103868-iwpiti2h 83 22 drug drug NN cord-103868-iwpiti2h 83 23 design design NN cord-103868-iwpiti2h 83 24 . . . cord-103868-iwpiti2h 84 1 354 354 CD cord-103868-iwpiti2h 84 2 355 355 CD cord-103868-iwpiti2h 85 1 The the DT cord-103868-iwpiti2h 85 2 construct construct NN cord-103868-iwpiti2h 85 3 to to TO cord-103868-iwpiti2h 85 4 express express VB cord-103868-iwpiti2h 85 5 the the DT cord-103868-iwpiti2h 85 6 minimal minimal JJ cord-103868-iwpiti2h 85 7 NLS nls NN cord-103868-iwpiti2h 85 8 from from IN cord-103868-iwpiti2h 85 9 human human JJ cord-103868-iwpiti2h 85 10 cytomegalovirus cytomegalovirus NN cord-103868-iwpiti2h 85 11 UL44 UL44 NNP cord-103868-iwpiti2h 85 12 protein protein NN cord-103868-iwpiti2h 85 13 358 358 CD cord-103868-iwpiti2h 86 1 ( ( -LRB- cord-103868-iwpiti2h 86 2 residues residue NNS cord-103868-iwpiti2h 86 3 425 425 CD cord-103868-iwpiti2h 86 4 - - SYM cord-103868-iwpiti2h 86 5 433 433 CD cord-103868-iwpiti2h 86 6 ) ) -RRB- cord-103868-iwpiti2h 86 7 fused fuse VBN cord-103868-iwpiti2h 86 8 to to IN cord-103868-iwpiti2h 86 9 GFP GFP NNP cord-103868-iwpiti2h 86 10 was be VBD cord-103868-iwpiti2h 86 11 generated generate VBN cord-103868-iwpiti2h 86 12 by by IN cord-103868-iwpiti2h 86 13 subcloning subclone VBG cord-103868-iwpiti2h 86 14 from from IN cord-103868-iwpiti2h 86 15 pEPI pEPI NNP cord-103868-iwpiti2h 86 16 - - HYPH cord-103868-iwpiti2h 86 17 GFP gfp NN cord-103868-iwpiti2h 86 18 - - HYPH cord-103868-iwpiti2h 86 19 UL44425 UL44425 VBN cord-103868-iwpiti2h 86 20 - - HYPH cord-103868-iwpiti2h 86 21 433 433 CD cord-103868-iwpiti2h 86 22 ( ( -LRB- cord-103868-iwpiti2h 86 23 42 42 CD cord-103868-iwpiti2h 86 24 , , , cord-103868-iwpiti2h 86 25 359 359 CD cord-103868-iwpiti2h 86 26 43 43 CD cord-103868-iwpiti2h 86 27 ) ) -RRB- cord-103868-iwpiti2h 86 28 into into IN cord-103868-iwpiti2h 86 29 the the DT cord-103868-iwpiti2h 86 30 pEGFP pEGFP NNP cord-103868-iwpiti2h 86 31 - - HYPH cord-103868-iwpiti2h 86 32 C1 c1 NN cord-103868-iwpiti2h 86 33 vector vector NN cord-103868-iwpiti2h 86 34 C c NN cord-103868-iwpiti2h 86 35 - - HYPH cord-103868-iwpiti2h 86 36 terminal terminal NN cord-103868-iwpiti2h 86 37 to to IN cord-103868-iwpiti2h 86 38 GFP GFP NNP cord-103868-iwpiti2h 86 39 ( ( -LRB- cord-103868-iwpiti2h 86 40 Clontech Clontech NNP cord-103868-iwpiti2h 86 41 ) ) -RRB- cord-103868-iwpiti2h 86 42 . . . cord-103868-iwpiti2h 87 1 Constructs construct NNS cord-103868-iwpiti2h 87 2 to to TO cord-103868-iwpiti2h 87 3 express express VB cord-103868-iwpiti2h 87 4 full full JJ cord-103868-iwpiti2h 87 5 - - HYPH cord-103868-iwpiti2h 87 6 length length NN cord-103868-iwpiti2h 87 7 360 360 CD cord-103868-iwpiti2h 87 8 or or CC cord-103868-iwpiti2h 87 9 truncated truncate VBN cord-103868-iwpiti2h 87 10 EBOV EBOV NNP cord-103868-iwpiti2h 87 11 - - HYPH cord-103868-iwpiti2h 87 12 VP24 VP24 NNP cord-103868-iwpiti2h 87 13 protein protein NN cord-103868-iwpiti2h 87 14 fused fuse VBN cord-103868-iwpiti2h 87 15 to to IN cord-103868-iwpiti2h 87 16 GFP GFP NNP cord-103868-iwpiti2h 87 17 or or CC cord-103868-iwpiti2h 87 18 GFP GFP NNP cord-103868-iwpiti2h 87 19 - - HYPH cord-103868-iwpiti2h 87 20 UL44NLS ul44nl NNS cord-103868-iwpiti2h 87 21 were be VBD cord-103868-iwpiti2h 87 22 generated generate VBN cord-103868-iwpiti2h 87 23 by by IN cord-103868-iwpiti2h 87 24 PCR PCR NNP cord-103868-iwpiti2h 87 25 to to TO cord-103868-iwpiti2h 87 26 express express VB cord-103868-iwpiti2h 87 27 FLAG flag NN cord-103868-iwpiti2h 87 28 - - HYPH cord-103868-iwpiti2h 87 29 tagged tag VBN cord-103868-iwpiti2h 87 30 K1 K1 NNP cord-103868-iwpiti2h 87 31 was be VBD cord-103868-iwpiti2h 87 32 a a DT cord-103868-iwpiti2h 87 33 kind kind JJ cord-103868-iwpiti2h 87 34 gift gift NN cord-103868-iwpiti2h 87 35 from from IN cord-103868-iwpiti2h 87 36 C. C. NNP cord-103868-iwpiti2h 87 37 Basler Basler NNP cord-103868-iwpiti2h 87 38 ( ( -LRB- cord-103868-iwpiti2h 87 39 Georgia Georgia NNP cord-103868-iwpiti2h 87 40 State State NNP cord-103868-iwpiti2h 87 41 University University NNP cord-103868-iwpiti2h 87 42 ) ) -RRB- cord-103868-iwpiti2h 87 43 . . . cord-103868-iwpiti2h 88 1 Other other JJ cord-103868-iwpiti2h 88 2 366 366 CD cord-103868-iwpiti2h 88 3 constructs construct NNS cord-103868-iwpiti2h 88 4 have have VBP cord-103868-iwpiti2h 88 5 been be VBN cord-103868-iwpiti2h 88 6 described describe VBN cord-103868-iwpiti2h 88 7 elsewhere elsewhere RB cord-103868-iwpiti2h 88 8 ( ( -LRB- cord-103868-iwpiti2h 88 9 40 40 CD cord-103868-iwpiti2h 88 10 ) ) -RRB- cord-103868-iwpiti2h 88 11 . . . cord-103868-iwpiti2h 88 12 _SP cord-103868-iwpiti2h 89 1 Discovery discovery NN cord-103868-iwpiti2h 89 2 of of IN cord-103868-iwpiti2h 89 3 an an DT cord-103868-iwpiti2h 89 4 Ebolavirus Ebolavirus NNP cord-103868-iwpiti2h 89 5 - - HYPH cord-103868-iwpiti2h 89 6 Like Like NNP cord-103868-iwpiti2h 89 7 Filovirus Filovirus NNP cord-103868-iwpiti2h 89 8 446 446 CD cord-103868-iwpiti2h 89 9 in in IN cord-103868-iwpiti2h 89 10 Europe Europe NNP cord-103868-iwpiti2h 89 11 Filoviruses Filoviruses NNPS cord-103868-iwpiti2h 89 12 : : : cord-103868-iwpiti2h 89 13 Ecology Ecology NNP cord-103868-iwpiti2h 89 14 , , , cord-103868-iwpiti2h 89 15 Molecular Molecular NNP cord-103868-iwpiti2h 89 16 Biology Biology NNP cord-103868-iwpiti2h 89 17 , , , cord-103868-iwpiti2h 89 18 and and CC cord-103868-iwpiti2h 89 19 448 448 CD cord-103868-iwpiti2h 89 20 Evolution evolution NN cord-103868-iwpiti2h 89 21 Descriptive descriptive JJ cord-103868-iwpiti2h 89 22 Analysis Analysis NNP cord-103868-iwpiti2h 89 23 of of IN cord-103868-iwpiti2h 89 24 Ebola Ebola NNP cord-103868-iwpiti2h 89 25 Virus Virus NNP cord-103868-iwpiti2h 89 26 Proteins Proteins NNP cord-103868-iwpiti2h 89 27 Infection infection NN cord-103868-iwpiti2h 89 28 of of IN cord-103868-iwpiti2h 89 29 naive naive JJ cord-103868-iwpiti2h 89 30 target target NN cord-103868-iwpiti2h 89 31 cells cell NNS cord-103868-iwpiti2h 89 32 with with IN cord-103868-iwpiti2h 89 33 virus virus NN cord-103868-iwpiti2h 89 34 - - : cord-103868-iwpiti2h 89 35 like like JJ cord-103868-iwpiti2h 89 36 453 453 CD cord-103868-iwpiti2h 89 37 particles particle NNS cord-103868-iwpiti2h 89 38 : : : cord-103868-iwpiti2h 89 39 implications implication NNS cord-103868-iwpiti2h 89 40 for for IN cord-103868-iwpiti2h 89 41 the the DT cord-103868-iwpiti2h 89 42 function function NN cord-103868-iwpiti2h 89 43 of of IN cord-103868-iwpiti2h 89 44 ebola ebola NNP cord-103868-iwpiti2h 89 45 virus virus NN cord-103868-iwpiti2h 89 46 VP24 VP24 NNP cord-103868-iwpiti2h 89 47 Knockdown Knockdown NNP cord-103868-iwpiti2h 89 48 of of IN cord-103868-iwpiti2h 89 49 Ebola Ebola NNP cord-103868-iwpiti2h 89 50 virus virus NN cord-103868-iwpiti2h 89 51 VP24 VP24 NNP cord-103868-iwpiti2h 89 52 impairs impair VBZ cord-103868-iwpiti2h 89 53 viral viral JJ cord-103868-iwpiti2h 89 54 nucleocapsid nucleocapsid NNP cord-103868-iwpiti2h 89 55 assembly assembly NNP cord-103868-iwpiti2h 89 56 and and CC cord-103868-iwpiti2h 89 57 456 456 CD cord-103868-iwpiti2h 89 58 prevents prevent VBZ cord-103868-iwpiti2h 89 59 virus virus NN cord-103868-iwpiti2h 89 60 replication replication NN cord-103868-iwpiti2h 90 1 The the DT cord-103868-iwpiti2h 90 2 assembly assembly NN cord-103868-iwpiti2h 90 3 of of IN cord-103868-iwpiti2h 90 4 Ebola Ebola NNP cord-103868-iwpiti2h 90 5 virus virus NN cord-103868-iwpiti2h 90 6 nucleocapsid nucleocapsid NN cord-103868-iwpiti2h 90 7 458 458 CD cord-103868-iwpiti2h 90 8 requires require VBZ cord-103868-iwpiti2h 90 9 virion virion NN cord-103868-iwpiti2h 90 10 - - HYPH cord-103868-iwpiti2h 90 11 associated associate VBN cord-103868-iwpiti2h 90 12 proteins protein NNS cord-103868-iwpiti2h 90 13 35 35 CD cord-103868-iwpiti2h 90 14 and and CC cord-103868-iwpiti2h 90 15 24 24 CD cord-103868-iwpiti2h 90 16 and and CC cord-103868-iwpiti2h 90 17 posttranslational posttranslational JJ cord-103868-iwpiti2h 90 18 modification modification NN cord-103868-iwpiti2h 90 19 of of IN cord-103868-iwpiti2h 90 20 54 54 CD cord-103868-iwpiti2h 90 21 Contribution Contribution NNP cord-103868-iwpiti2h 90 22 of of IN cord-103868-iwpiti2h 90 23 ebola ebola NNP cord-103868-iwpiti2h 90 24 virus virus NN cord-103868-iwpiti2h 90 25 608 608 CD cord-103868-iwpiti2h 90 26 glycoprotein glycoprotein NN cord-103868-iwpiti2h 90 27 , , , cord-103868-iwpiti2h 90 28 nucleoprotein nucleoprotein NNP cord-103868-iwpiti2h 90 29 , , , cord-103868-iwpiti2h 90 30 and and CC cord-103868-iwpiti2h 90 31 VP24 VP24 NNP cord-103868-iwpiti2h 90 32 to to IN cord-103868-iwpiti2h 90 33 budding bud VBG cord-103868-iwpiti2h 90 34 of of IN cord-103868-iwpiti2h 90 35 VP40 VP40 NNP cord-103868-iwpiti2h 90 36 virus virus NN cord-103868-iwpiti2h 90 37 - - HYPH cord-103868-iwpiti2h 90 38 like like JJ cord-103868-iwpiti2h 90 39 particles particle NNS cord-103868-iwpiti2h 91 1 Both both DT cord-103868-iwpiti2h 91 2 matrix matrix NN cord-103868-iwpiti2h 91 3 proteins protein NNS cord-103868-iwpiti2h 91 4 of of IN cord-103868-iwpiti2h 91 5 Ebola Ebola NNP cord-103868-iwpiti2h 91 6 611 611 CD cord-103868-iwpiti2h 91 7 virus virus NN cord-103868-iwpiti2h 91 8 contribute contribute VBP cord-103868-iwpiti2h 91 9 to to IN cord-103868-iwpiti2h 91 10 the the DT cord-103868-iwpiti2h 91 11 regulation regulation NN cord-103868-iwpiti2h 91 12 of of IN cord-103868-iwpiti2h 91 13 viral viral JJ cord-103868-iwpiti2h 91 14 genome genome NN cord-103868-iwpiti2h 91 15 replication replication NN cord-103868-iwpiti2h 91 16 and and CC cord-103868-iwpiti2h 91 17 transcription transcription NN cord-103868-iwpiti2h 91 18 Ebola Ebola NNP cord-103868-iwpiti2h 91 19 virus virus NN cord-103868-iwpiti2h 91 20 614 614 CD cord-103868-iwpiti2h 92 1 ( ( -LRB- cord-103868-iwpiti2h 92 2 EBOV EBOV NNP cord-103868-iwpiti2h 92 3 ) ) -RRB- cord-103868-iwpiti2h 93 1 VP24 VP24 NNP cord-103868-iwpiti2h 93 2 inhibits inhibit VBZ cord-103868-iwpiti2h 93 3 transcription transcription NN cord-103868-iwpiti2h 93 4 and and CC cord-103868-iwpiti2h 93 5 replication replication NN cord-103868-iwpiti2h 93 6 of of IN cord-103868-iwpiti2h 93 7 the the DT cord-103868-iwpiti2h 93 8 EBOV EBOV NNP cord-103868-iwpiti2h 93 9 genome genome NN cord-103868-iwpiti2h 94 1 VP24 VP24 NNP cord-103868-iwpiti2h 94 2 Is be VBZ cord-103868-iwpiti2h 94 3 a a DT cord-103868-iwpiti2h 94 4 Molecular molecular JJ cord-103868-iwpiti2h 94 5 Determinant determinant NN cord-103868-iwpiti2h 94 6 of of IN cord-103868-iwpiti2h 94 7 Ebola Ebola NNP cord-103868-iwpiti2h 94 8 Virus Virus NNP cord-103868-iwpiti2h 94 9 Virulence Virulence NNP cord-103868-iwpiti2h 94 10 618 618 CD cord-103868-iwpiti2h 94 11 in in IN cord-103868-iwpiti2h 94 12 Guinea Guinea NNP cord-103868-iwpiti2h 94 13 Pigs Pigs NNP cord-103868-iwpiti2h 95 1 A a DT cord-103868-iwpiti2h 95 2 single single JJ cord-103868-iwpiti2h 95 3 phosphorodiamidate phosphorodiamidate JJ cord-103868-iwpiti2h 95 4 morpholino morpholino NNP cord-103868-iwpiti2h 95 5 621 621 CD cord-103868-iwpiti2h 95 6 oligomer oligomer NN cord-103868-iwpiti2h 95 7 targeting target VBG cord-103868-iwpiti2h 95 8 VP24 VP24 NNP cord-103868-iwpiti2h 96 1 protects protect VBZ cord-103868-iwpiti2h 96 2 rhesus rhesus NN cord-103868-iwpiti2h 96 3 monkeys monkey NNS cord-103868-iwpiti2h 96 4 against against IN cord-103868-iwpiti2h 96 5 lethal lethal JJ cord-103868-iwpiti2h 96 6 Ebola Ebola NNP cord-103868-iwpiti2h 96 7 virus virus NN cord-103868-iwpiti2h 96 8 infection infection NN cord-103868-iwpiti2h 96 9 Fn Fn NNP cord-103868-iwpiti2h 96 10 / / SYM cord-103868-iwpiti2h 96 11 c c NN cord-103868-iwpiti2h 96 12 ) ) -RRB- cord-103868-iwpiti2h 96 13 for for IN cord-103868-iwpiti2h 96 14 GFP GFP NNP cord-103868-iwpiti2h 97 1 ( ( -LRB- cord-103868-iwpiti2h 97 2 mean mean VB cord-103868-iwpiti2h 97 3 ± ± NNP cord-103868-iwpiti2h 97 4 SEM SEM NNP cord-103868-iwpiti2h 97 5 , , , cord-103868-iwpiti2h 97 6 n n NNP cord-103868-iwpiti2h 97 7   NNP cord-103868-iwpiti2h 97 8 52 52 CD cord-103868-iwpiti2h 97 9 637 637 CD cord-103868-iwpiti2h 97 10 cells cell NNS cord-103868-iwpiti2h 97 11 for for IN cord-103868-iwpiti2h 97 12 each each DT cord-103868-iwpiti2h 97 13 condition condition NN cord-103868-iwpiti2h 97 14 ; ; : cord-103868-iwpiti2h 97 15 results result NNS cord-103868-iwpiti2h 97 16 are be VBP cord-103868-iwpiti2h 97 17 from from IN cord-103868-iwpiti2h 97 18 a a DT cord-103868-iwpiti2h 97 19 single single JJ cord-103868-iwpiti2h 97 20 assay assay NN cord-103868-iwpiti2h 97 21 representative representative NN cord-103868-iwpiti2h 97 22 of of IN cord-103868-iwpiti2h 97 23 three three CD cord-103868-iwpiti2h 97 24 independent independent JJ cord-103868-iwpiti2h 97 25 638 638 CD cord-103868-iwpiti2h 97 26 assays assay NNS cord-103868-iwpiti2h 97 27 ) ) -RRB- cord-103868-iwpiti2h 97 28 . . . cord-103868-iwpiti2h 98 1 Statistical statistical JJ cord-103868-iwpiti2h 98 2 analysis analysis NN cord-103868-iwpiti2h 98 3 ( ( -LRB- cord-103868-iwpiti2h 98 4 Student student NN cord-103868-iwpiti2h 98 5 's 's POS cord-103868-iwpiti2h 98 6 t t NN cord-103868-iwpiti2h 98 7 - - HYPH cord-103868-iwpiti2h 98 8 test test NN cord-103868-iwpiti2h 98 9 ) ) -RRB- cord-103868-iwpiti2h 98 10 was be VBD cord-103868-iwpiti2h 98 11 performed perform VBN cord-103868-iwpiti2h 98 12 using use VBG cord-103868-iwpiti2h 98 13 GraphPad GraphPad NNP cord-103868-iwpiti2h 98 14 Prism Prism NNP cord-103868-iwpiti2h 98 15 software software NN cord-103868-iwpiti2h 98 16 A a NN cord-103868-iwpiti2h 98 17 ) ) -RRB- cord-103868-iwpiti2h 98 18 Schematic schematic NN cord-103868-iwpiti2h 98 19 of of IN cord-103868-iwpiti2h 98 20 full-642 full-642 JJ cord-103868-iwpiti2h 98 21 length length NN cord-103868-iwpiti2h 99 1 VP24 VP24 NNP cord-103868-iwpiti2h 99 2 and and CC cord-103868-iwpiti2h 99 3 truncated truncate VBD cord-103868-iwpiti2h 99 4 VP24 VP24 NNP cord-103868-iwpiti2h 99 5 proteins protein NNS cord-103868-iwpiti2h 99 6 generated generate VBN cord-103868-iwpiti2h 99 7 . . . cord-103868-iwpiti2h 100 1 Location location NN cord-103868-iwpiti2h 100 2 of of IN cord-103868-iwpiti2h 100 3 potential potential JJ cord-103868-iwpiti2h 100 4 NESs nes NNS cord-103868-iwpiti2h 100 5 are be VBP cord-103868-iwpiti2h 100 6 shown show VBN cord-103868-iwpiti2h 100 7 in in IN cord-103868-iwpiti2h 100 8 643 643 CD cord-103868-iwpiti2h 100 9 yellow yellow NN cord-103868-iwpiti2h 100 10 . . . cord-103868-iwpiti2h 101 1 Location location NN cord-103868-iwpiti2h 101 2 of of IN cord-103868-iwpiti2h 101 3 clusters cluster NNS cord-103868-iwpiti2h 101 4 ( ( -LRB- cord-103868-iwpiti2h 101 5 CL1 CL1 NNP cord-103868-iwpiti2h 101 6 - - HYPH cord-103868-iwpiti2h 101 7 3 3 NNP cord-103868-iwpiti2h 101 8 ) ) -RRB- cord-103868-iwpiti2h 101 9 of of IN cord-103868-iwpiti2h 101 10 residues residue NNS cord-103868-iwpiti2h 101 11 that that WDT cord-103868-iwpiti2h 101 12 interact interact VBP cord-103868-iwpiti2h 101 13 with with IN cord-103868-iwpiti2h 101 14 Ks ks NN cord-103868-iwpiti2h 101 15 in in IN cord-103868-iwpiti2h 101 16 the the DT cord-103868-iwpiti2h 101 17 VP24 VP24 NNP cord-103868-iwpiti2h 101 18 : : : cord-103868-iwpiti2h 101 19 Kα5 Kα5 NNP cord-103868-iwpiti2h 101 20 644 644 CD cord-103868-iwpiti2h 101 21 complex complex JJ cord-103868-iwpiti2h 101 22 crystal crystal NN cord-103868-iwpiti2h 101 23 structure structure NN cord-103868-iwpiti2h 101 24 ( ( -LRB- cord-103868-iwpiti2h 101 25 20 20 CD cord-103868-iwpiti2h 101 26 ) ) -RRB- cord-103868-iwpiti2h 101 27 are be VBP cord-103868-iwpiti2h 101 28 shown show VBN cord-103868-iwpiti2h 101 29 in in IN cord-103868-iwpiti2h 101 30 red red NN cord-103868-iwpiti2h 101 31 . . . cord-103868-iwpiti2h 102 1 Numbering numbering NN cord-103868-iwpiti2h 102 2 indicates indicate VBZ cord-103868-iwpiti2h 102 3 residue residue JJ cord-103868-iwpiti2h 102 4 positions position NNS cord-103868-iwpiti2h 102 5 in in IN cord-103868-iwpiti2h 102 6 full-645 full-645 JJ cord-103868-iwpiti2h 102 7 length length NN cord-103868-iwpiti2h 102 8 VP24 vp24 NN cord-103868-iwpiti2h 102 9 ; ; : cord-103868-iwpiti2h 102 10 sequences sequence NNS cord-103868-iwpiti2h 102 11 of of IN cord-103868-iwpiti2h 102 12 potential potential JJ cord-103868-iwpiti2h 102 13 NESs nes NNS cord-103868-iwpiti2h 102 14 are be VBP cord-103868-iwpiti2h 102 15 shown show VBN cord-103868-iwpiti2h 102 16 above above RB cord-103868-iwpiti2h 102 17 . . . cord-103868-iwpiti2h 103 1 ( ( -LRB- cord-103868-iwpiti2h 103 2 B b NN cord-103868-iwpiti2h 103 3 ) ) -RRB- cord-103868-iwpiti2h 103 4 COS7 COS7 NNP cord-103868-iwpiti2h 103 5 cells cell NNS cord-103868-iwpiti2h 104 1 Representative representative JJ cord-103868-iwpiti2h 104 2 images image NNS cord-103868-iwpiti2h 104 3 are be VBP cord-103868-iwpiti2h 104 4 shown show VBN cord-103868-iwpiti2h 104 5 . . . cord-103868-iwpiti2h 105 1 ( ( -LRB- cord-103868-iwpiti2h 105 2 C c NN cord-103868-iwpiti2h 105 3 ) ) -RRB- cord-103868-iwpiti2h 105 4 Images image NNS cord-103868-iwpiti2h 105 5 such such JJ cord-103868-iwpiti2h 105 6 648 648 CD cord-103868-iwpiti2h 105 7 as as IN cord-103868-iwpiti2h 105 8 those those DT cord-103868-iwpiti2h 105 9 shown show VBN cord-103868-iwpiti2h 105 10 in in IN cord-103868-iwpiti2h 105 11 B B NNP cord-103868-iwpiti2h 105 12 were be VBD cord-103868-iwpiti2h 105 13 analysed analyse VBN cord-103868-iwpiti2h 105 14 to to TO cord-103868-iwpiti2h 105 15 calculate calculate VB cord-103868-iwpiti2h 105 16 the the DT cord-103868-iwpiti2h 105 17 Fn Fn NNP cord-103868-iwpiti2h 105 18 / / SYM cord-103868-iwpiti2h 105 19 c c NN cord-103868-iwpiti2h 105 20 for for IN cord-103868-iwpiti2h 105 21 GFP GFP NNP cord-103868-iwpiti2h 105 22 ( ( -LRB- cord-103868-iwpiti2h 105 23 C C NNP cord-103868-iwpiti2h 106 1 ; ; : cord-103868-iwpiti2h 106 2 mean mean VB cord-103868-iwpiti2h 106 3 ± ± NNP cord-103868-iwpiti2h 106 4 SEM SEM NNP cord-103868-iwpiti2h 106 5 ; ; : cord-103868-iwpiti2h 106 6 n n NNP cord-103868-iwpiti2h 106 7   NNP cord-103868-iwpiti2h 106 8 31 31 CD cord-103868-iwpiti2h 106 9 cells cell NNS cord-103868-iwpiti2h 106 10 649 649 CD cord-103868-iwpiti2h 106 11 for for IN cord-103868-iwpiti2h 106 12 each each DT cord-103868-iwpiti2h 106 13 condition condition NN cord-103868-iwpiti2h 106 14 Statistical statistical JJ cord-103868-iwpiti2h 106 15 analysis analysis NN cord-103868-iwpiti2h 106 16 used use VBD cord-103868-iwpiti2h 106 17 Student Student NNP cord-103868-iwpiti2h 106 18 's 's POS cord-103868-iwpiti2h 106 19 t t NN cord-103868-iwpiti2h 106 20 - - HYPH cord-103868-iwpiti2h 106 21 test test NN cord-103868-iwpiti2h 106 22 . . . cord-103868-iwpiti2h 107 1 * * NFP cord-103868-iwpiti2h 107 2 * * NFP cord-103868-iwpiti2h 107 3 * * NFP cord-103868-iwpiti2h 107 4 * * NFP cord-103868-iwpiti2h 107 5 from from IN cord-103868-iwpiti2h 107 6 a a DT cord-103868-iwpiti2h 107 7 single single JJ cord-103868-iwpiti2h 107 8 assay assay NN cord-103868-iwpiti2h 107 9 representative representative NN cord-103868-iwpiti2h 107 10 of of IN cord-103868-iwpiti2h 107 11 two two CD cord-103868-iwpiti2h 107 12 independent independent JJ cord-103868-iwpiti2h 107 13 assays assay NNS cord-103868-iwpiti2h 107 14 ) ) -RRB- cord-103868-iwpiti2h 107 15 . . . cord-103868-iwpiti2h 108 1 Statistical statistical JJ cord-103868-iwpiti2h 108 2 analysis analysis NN cord-103868-iwpiti2h 108 3 used use VBD cord-103868-iwpiti2h 108 4 657 657 CD cord-103868-iwpiti2h 108 5 Student Student NNP cord-103868-iwpiti2h 108 6 's 's POS cord-103868-iwpiti2h 108 7 t t NN cord-103868-iwpiti2h 108 8 - - HYPH cord-103868-iwpiti2h 108 9 test test NN cord-103868-iwpiti2h 109 1 The the DT cord-103868-iwpiti2h 109 2 authors author NNS cord-103868-iwpiti2h 109 3 declare declare VBP cord-103868-iwpiti2h 109 4 that that IN cord-103868-iwpiti2h 109 5 they -PRON- PRP cord-103868-iwpiti2h 109 6 have have VBP cord-103868-iwpiti2h 109 7 no no DT cord-103868-iwpiti2h 109 8 conflicts conflict NNS cord-103868-iwpiti2h 109 9 of of IN cord-103868-iwpiti2h 109 10 interest interest NN cord-103868-iwpiti2h 109 11 with with IN cord-103868-iwpiti2h 109 12 the the DT cord-103868-iwpiti2h 109 13 contents content NNS cord-103868-iwpiti2h 109 14 of of IN cord-103868-iwpiti2h 109 15 this this DT cord-103868-iwpiti2h 109 16 article article NN cord-103868-iwpiti2h 109 17 . . . cord-103868-iwpiti2h 110 1 441 441 CD