id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-007261-b5fgb9wf	Murakami, Kazuya	The Transmembrane Region of Microsomal Cytochrome P450 Identified as the Endoplasmic Reticulum Retention Signal(1)	1994-07-17		.txt	text/plain	6977	324	55	Another chimeric protein construct in which carboxyesterase Sec was connected to the C-terminal region of rat UDP-glucuronosyltransferase (UDP-GT), that contained a double-lysin ER retention motif, was also located in the ER, as determined from the endo H sensitivity and immunofluorescence staining. As a control, other types of fusion proteins were constructed in which the C-terminal portion of UDPglucronosyltransferase, which contains the double-lysine motif for the ER retention, was attached to carboxyesterase Sec. The fusion proteins were expressed in COS cells, and the subcellular localization of the fusion proteins was determined. To determine whether P450s are retained permanently in the ER or recycled between the ER and post-ER compartments through the "export and retrieval" process, we chose a lysosomal enzyme, cathepsin D, as a reporter, which was fused to the N-terminus of P450 at the DNA level and the fusion protein was expressed in COS cells.	./cache/cord-007261-b5fgb9wf.txt	./txt/cord-007261-b5fgb9wf.txt