id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-007353-qg2pb884	Lavi, Ehud	Polarity of processes with Golgi apparatus in a subpopulation of type I astrocytes	1994-06-06		.txt	text/plain	6266	321	46	In order to further investigate which type of astrocytes contain GA in processes we conducted the present study using primary cultures of rat astrocytes and organelle specific antibodies against the GA and the rough endoplasmic reticulum (RER). In a previous ultrastructural, immunocytochemical study from this laboratory, using affinity-purified polyclonal sera specific for the GA, elements of the organelle were detected in peripheral segments of some astrocytic processes in sections of rat brains [68] . Over 95% of the cultured cells stained with the monoclonal antibody against GFAP [43] , an astrocyte specific intermediate filament protein. Staining of MG-160 in processes in rat astrocyte enriched cultures was detected by immunohistochemistry and immunofluorescence with both polyclonal and monoclonal antibodies against MG-160. To confirm that cells which expressed MG-160 in processes were astrocytes, double-labeling immunofluorescence with anti-MG-160 and astrocytic-specific intermediate filament marker (GFAP) antibodies were performed.	./cache/cord-007353-qg2pb884.txt	./txt/cord-007353-qg2pb884.txt