id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-277566-j3ehiwn9	Verheije, Monique H.	Mouse Hepatitis Coronavirus RNA Replication Depends on GBF1-Mediated ARF1 Activation	2008-06-13		.txt	text/plain	8918	449	49	Here, we study the involvement of the secretory pathway in mouse hepatitis coronavirus (MHV) replication by using the drug brefeldin A (BFA), which is known to interfere with ER-Golgi membrane traffic by inhibiting the activation of ADP-ribosylation factor (ARF) small GTPases. Therefore, LR7 cells infected with MHV-A59 were treated with BFA for 30 minutes starting 5.5 h p.i. They were subsequently fixed and processed for immunofluorescence using antibodies both against nsp8, which served as a protein marker for the MHV replication sites [50, 51] , and against the viral structural protein M, known to reside in the Golgi [52] . In complete agreement with the luciferase expression data shown above, this result demonstrates that BFA inhibits, but does not completely block, the formation of RCs. To study the effects of BFA on the DMVs at an ultrastructural level, MHV-infected LR7 cells were fixed at 6 h p.i. and embedded in Epon resin in order to be analyzed by electron microscopy.	./cache/cord-277566-j3ehiwn9.txt	./txt/cord-277566-j3ehiwn9.txt