id sid tid token lemma pos cord-022774-wasdp2gh 1 1 key key JJ cord-022774-wasdp2gh 2 1 : : : cord-022774-wasdp2gh 2 2 cord-022774-wasdp2gh cord-022774-wasdp2gh NNP cord-022774-wasdp2gh 2 3 authors author NNS cord-022774-wasdp2gh 2 4 : : : cord-022774-wasdp2gh 2 5 Morré Morré NNP cord-022774-wasdp2gh 2 6 , , , cord-022774-wasdp2gh 2 7 D. D. NNP cord-022774-wasdp2gh 2 8 James James NNP cord-022774-wasdp2gh 2 9 ; ; : cord-022774-wasdp2gh 2 10 Minnifield Minnifield NNP cord-022774-wasdp2gh 2 11 , , , cord-022774-wasdp2gh 2 12 Nita Nita NNP cord-022774-wasdp2gh 2 13 ; ; : cord-022774-wasdp2gh 2 14 Paulik Paulik NNP cord-022774-wasdp2gh 2 15 , , , cord-022774-wasdp2gh 2 16 Mark Mark NNP cord-022774-wasdp2gh 2 17 title title NN cord-022774-wasdp2gh 2 18 : : : cord-022774-wasdp2gh 2 19 Identification identification NN cord-022774-wasdp2gh 2 20 of of IN cord-022774-wasdp2gh 2 21 the the DT cord-022774-wasdp2gh 2 22 16 16 CD cord-022774-wasdp2gh 2 23 ° ° NN cord-022774-wasdp2gh 2 24 C C NNP cord-022774-wasdp2gh 2 25 compartment compartment NN cord-022774-wasdp2gh 2 26 of of IN cord-022774-wasdp2gh 2 27 the the DT cord-022774-wasdp2gh 2 28 endoplasmic endoplasmic NN cord-022774-wasdp2gh 2 29 reticulum reticulum NN cord-022774-wasdp2gh 2 30 in in IN cord-022774-wasdp2gh 2 31 rat rat NN cord-022774-wasdp2gh 2 32 liver liver NN cord-022774-wasdp2gh 2 33 and and CC cord-022774-wasdp2gh 2 34 cultured cultured JJ cord-022774-wasdp2gh 2 35 hamster hamster NN cord-022774-wasdp2gh 2 36 kidney kidney NN cord-022774-wasdp2gh 2 37 cells cell NNS cord-022774-wasdp2gh 2 38 date date NN cord-022774-wasdp2gh 2 39 : : : cord-022774-wasdp2gh 2 40 2012 2012 CD cord-022774-wasdp2gh 2 41 - - HYPH cord-022774-wasdp2gh 2 42 02 02 CD cord-022774-wasdp2gh 2 43 - - HYPH cord-022774-wasdp2gh 2 44 07 07 CD cord-022774-wasdp2gh 2 45 journal journal NN cord-022774-wasdp2gh 2 46 : : : cord-022774-wasdp2gh 2 47 Biol Biol NNP cord-022774-wasdp2gh 2 48 Cell Cell NNP cord-022774-wasdp2gh 2 49 DOI DOI NNP cord-022774-wasdp2gh 2 50 : : : cord-022774-wasdp2gh 2 51 10.1111 10.1111 CD cord-022774-wasdp2gh 2 52 / / SYM cord-022774-wasdp2gh 2 53 j.1768 j.1768 NNP cord-022774-wasdp2gh 2 54 - - HYPH cord-022774-wasdp2gh 2 55 322x.1989.tb03009.x 322x.1989.tb03009.x NNP cord-022774-wasdp2gh 2 56 sha sha NN cord-022774-wasdp2gh 2 57 : : : cord-022774-wasdp2gh 2 58 b57b131ba8ea531242be099c1b4c1c7fa5247bfe b57b131ba8ea531242be099c1b4c1c7fa5247bfe NN cord-022774-wasdp2gh 2 59 doc_id doc_id CD cord-022774-wasdp2gh 2 60 : : : cord-022774-wasdp2gh 2 61 22774 22774 CD cord-022774-wasdp2gh 3 1 cord_uid cord_uid NN cord-022774-wasdp2gh 3 2 : : : cord-022774-wasdp2gh 4 1 wasdp2gh wasdp2gh NNP cord-022774-wasdp2gh 4 2 Summary Summary NNP cord-022774-wasdp2gh 4 3 — — : cord-022774-wasdp2gh 4 4 In in IN cord-022774-wasdp2gh 4 5 many many JJ cord-022774-wasdp2gh 4 6 systems system NNS cord-022774-wasdp2gh 4 7 transfer transfer VBP cord-022774-wasdp2gh 4 8 between between IN cord-022774-wasdp2gh 4 9 the the DT cord-022774-wasdp2gh 4 10 endoplasmic endoplasmic NN cord-022774-wasdp2gh 4 11 reticulum reticulum NN cord-022774-wasdp2gh 4 12 and and CC cord-022774-wasdp2gh 4 13 the the DT cord-022774-wasdp2gh 4 14 Golgi Golgi NNP cord-022774-wasdp2gh 4 15 apparatus apparatus NN cord-022774-wasdp2gh 4 16 is be VBZ cord-022774-wasdp2gh 4 17 blocked block VBN cord-022774-wasdp2gh 4 18 at at IN cord-022774-wasdp2gh 4 19 temperatures temperature NNS cord-022774-wasdp2gh 4 20 below below IN cord-022774-wasdp2gh 4 21 16 16 CD cord-022774-wasdp2gh 4 22 ° ° NN cord-022774-wasdp2gh 4 23 C c NN cord-022774-wasdp2gh 4 24 . . . cord-022774-wasdp2gh 5 1 In in IN cord-022774-wasdp2gh 5 2 virus‐infected virus‐infected JJ cord-022774-wasdp2gh 5 3 cells cell NNS cord-022774-wasdp2gh 5 4 in in IN cord-022774-wasdp2gh 5 5 culture culture NN cord-022774-wasdp2gh 5 6 , , , cord-022774-wasdp2gh 5 7 a a DT cord-022774-wasdp2gh 5 8 special special JJ cord-022774-wasdp2gh 5 9 membrane membrane NN cord-022774-wasdp2gh 5 10 compartment compartment NN cord-022774-wasdp2gh 5 11 is be VBZ cord-022774-wasdp2gh 5 12 seen see VBN cord-022774-wasdp2gh 5 13 to to TO cord-022774-wasdp2gh 5 14 accumulate accumulate VB cord-022774-wasdp2gh 5 15 . . . cord-022774-wasdp2gh 6 1 Our -PRON- PRP$ cord-022774-wasdp2gh 6 2 studies study NNS cord-022774-wasdp2gh 6 3 with with IN cord-022774-wasdp2gh 6 4 rat rat NN cord-022774-wasdp2gh 6 5 liver liver NNP cord-022774-wasdp2gh 6 6 show show VBP cord-022774-wasdp2gh 6 7 a a DT cord-022774-wasdp2gh 6 8 similar similar JJ cord-022774-wasdp2gh 6 9 response response NN cord-022774-wasdp2gh 6 10 to to IN cord-022774-wasdp2gh 6 11 temperature temperature NN cord-022774-wasdp2gh 6 12 both both DT cord-022774-wasdp2gh 6 13 in in IN cord-022774-wasdp2gh 6 14 situ situ NN cord-022774-wasdp2gh 6 15 with with IN cord-022774-wasdp2gh 6 16 slices slice NNS cord-022774-wasdp2gh 6 17 and and CC cord-022774-wasdp2gh 6 18 in in IN cord-022774-wasdp2gh 6 19 vitro vitro FW cord-022774-wasdp2gh 6 20 with with IN cord-022774-wasdp2gh 6 21 isolated isolate VBN cord-022774-wasdp2gh 6 22 transitional transitional JJ cord-022774-wasdp2gh 6 23 endoplasmic endoplasmic NN cord-022774-wasdp2gh 6 24 relticulum relticulum NN cord-022774-wasdp2gh 6 25 fractions fraction NNS cord-022774-wasdp2gh 6 26 . . . cord-022774-wasdp2gh 7 1 With with IN cord-022774-wasdp2gh 7 2 isolated isolate VBN cord-022774-wasdp2gh 7 3 transitional transitional JJ cord-022774-wasdp2gh 7 4 endoplasmic endoplasmic NN cord-022774-wasdp2gh 7 5 reticulum reticulum NN cord-022774-wasdp2gh 7 6 fractions fraction NNS cord-022774-wasdp2gh 7 7 , , , cord-022774-wasdp2gh 7 8 when when WRB cord-022774-wasdp2gh 7 9 incubated incubate VBN cord-022774-wasdp2gh 7 10 in in IN cord-022774-wasdp2gh 7 11 the the DT cord-022774-wasdp2gh 7 12 presence presence NN cord-022774-wasdp2gh 7 13 of of IN cord-022774-wasdp2gh 7 14 nucleoside nucleoside NN cord-022774-wasdp2gh 7 15 triphosphate triphosphate NN cord-022774-wasdp2gh 7 16 and and CC cord-022774-wasdp2gh 7 17 a a DT cord-022774-wasdp2gh 7 18 cytosol cytosol NN cord-022774-wasdp2gh 7 19 fraction fraction NN cord-022774-wasdp2gh 7 20 , , , cord-022774-wasdp2gh 7 21 temperature temperature NN cord-022774-wasdp2gh 7 22 dependent dependent JJ cord-022774-wasdp2gh 7 23 formation formation NN cord-022774-wasdp2gh 7 24 of of IN cord-022774-wasdp2gh 7 25 vesicles vesicle NNS cord-022774-wasdp2gh 7 26 occurred occur VBD cord-022774-wasdp2gh 7 27 with with IN cord-022774-wasdp2gh 7 28 a a DT cord-022774-wasdp2gh 7 29 Q(10 Q(10 NNP cord-022774-wasdp2gh 7 30 ) ) -RRB- cord-022774-wasdp2gh 7 31 of of IN cord-022774-wasdp2gh 7 32 −2 −2 NNP cord-022774-wasdp2gh 7 33 but but CC cord-022774-wasdp2gh 7 34 was be VBD cord-022774-wasdp2gh 7 35 apparent apparent JJ cord-022774-wasdp2gh 7 36 only only RB cord-022774-wasdp2gh 7 37 at at IN cord-022774-wasdp2gh 7 38 temperatures temperature NNS cord-022774-wasdp2gh 7 39 greater great JJR cord-022774-wasdp2gh 7 40 than than IN cord-022774-wasdp2gh 7 41 12 12 CD cord-022774-wasdp2gh 7 42 ° ° NN cord-022774-wasdp2gh 7 43 C c NN cord-022774-wasdp2gh 7 44 . . . cord-022774-wasdp2gh 8 1 A a DT cord-022774-wasdp2gh 8 2 similar similar JJ cord-022774-wasdp2gh 8 3 response response NN cord-022774-wasdp2gh 8 4 was be VBD cord-022774-wasdp2gh 8 5 seen see VBN cord-022774-wasdp2gh 8 6 in in IN cord-022774-wasdp2gh 8 7 situ situ NNS cord-022774-wasdp2gh 8 8 at at IN cord-022774-wasdp2gh 8 9 12 12 CD cord-022774-wasdp2gh 8 10 ° ° NN cord-022774-wasdp2gh 8 11 C c NN cord-022774-wasdp2gh 8 12 and and CC cord-022774-wasdp2gh 8 13 16 16 CD cord-022774-wasdp2gh 8 14 ° ° NN cord-022774-wasdp2gh 8 15 C c NN cord-022774-wasdp2gh 8 16 where where WRB cord-022774-wasdp2gh 8 17 fusion fusion NN cord-022774-wasdp2gh 8 18 of of IN cord-022774-wasdp2gh 8 19 transition transition NN cord-022774-wasdp2gh 8 20 vesicles vesicle NNS cord-022774-wasdp2gh 8 21 with with IN cord-022774-wasdp2gh 8 22 cis cis NNP cord-022774-wasdp2gh 8 23 Golgi Golgi NNP cord-022774-wasdp2gh 8 24 apparatus apparatus NN cord-022774-wasdp2gh 8 25 , , , cord-022774-wasdp2gh 8 26 but but CC cord-022774-wasdp2gh 8 27 not not RB cord-022774-wasdp2gh 8 28 their -PRON- PRP$ cord-022774-wasdp2gh 8 29 formation formation NN cord-022774-wasdp2gh 8 30 , , , cord-022774-wasdp2gh 8 31 was be VBD cord-022774-wasdp2gh 8 32 blocked block VBN cord-022774-wasdp2gh 8 33 and and CC cord-022774-wasdp2gh 8 34 transition transition NN cord-022774-wasdp2gh 8 35 vesicles vesicle NNS cord-022774-wasdp2gh 8 36 accumulated accumulate VBN cord-022774-wasdp2gh 8 37 in in IN cord-022774-wasdp2gh 8 38 large large JJ cord-022774-wasdp2gh 8 39 numbers number NNS cord-022774-wasdp2gh 8 40 . . . cord-022774-wasdp2gh 9 1 At at IN cord-022774-wasdp2gh 9 2 18 18 CD cord-022774-wasdp2gh 9 3 ° ° NN cord-022774-wasdp2gh 9 4 C c NN cord-022774-wasdp2gh 9 5 and and CC cord-022774-wasdp2gh 9 6 below below RB cord-022774-wasdp2gh 9 7 and and CC cord-022774-wasdp2gh 9 8 especially especially RB cord-022774-wasdp2gh 9 9 at at IN cord-022774-wasdp2gh 9 10 8 8 CD cord-022774-wasdp2gh 9 11 ° ° NN cord-022774-wasdp2gh 9 12 C c NN cord-022774-wasdp2gh 9 13 and and CC cord-022774-wasdp2gh 9 14 12 12 CD cord-022774-wasdp2gh 9 15 ° ° NN cord-022774-wasdp2gh 9 16 C c NN cord-022774-wasdp2gh 9 17 , , , cord-022774-wasdp2gh 9 18 the the DT cord-022774-wasdp2gh 9 19 cells cell NNS cord-022774-wasdp2gh 9 20 responded respond VBD cord-022774-wasdp2gh 9 21 by by IN cord-022774-wasdp2gh 9 22 accumulating accumulate VBG cord-022774-wasdp2gh 9 23 smooth smooth JJ cord-022774-wasdp2gh 9 24 tubular tubular JJ cord-022774-wasdp2gh 9 25 transitional transitional JJ cord-022774-wasdp2gh 9 26 membranes membrane NNS cord-022774-wasdp2gh 9 27 near near IN cord-022774-wasdp2gh 9 28 the the DT cord-022774-wasdp2gh 9 29 cis cis NNP cord-022774-wasdp2gh 9 30 Golgi Golgi NNP cord-022774-wasdp2gh 9 31 apparatus apparatus NN cord-022774-wasdp2gh 9 32 face face NN cord-022774-wasdp2gh 9 33 . . . cord-022774-wasdp2gh 10 1 With with IN cord-022774-wasdp2gh 10 2 cells cell NNS cord-022774-wasdp2gh 10 3 and and CC cord-022774-wasdp2gh 10 4 tissue tissue NN cord-022774-wasdp2gh 10 5 slices slice NNS cord-022774-wasdp2gh 10 6 at at IN cord-022774-wasdp2gh 10 7 20 20 CD cord-022774-wasdp2gh 10 8 ° ° NN cord-022774-wasdp2gh 10 9 C c NN cord-022774-wasdp2gh 10 10 neither neither CC cord-022774-wasdp2gh 10 11 transition transition NN cord-022774-wasdp2gh 10 12 vesicles vesicle NNS cord-022774-wasdp2gh 10 13 nor nor CC cord-022774-wasdp2gh 10 14 the the DT cord-022774-wasdp2gh 10 15 smooth smooth JJ cord-022774-wasdp2gh 10 16 tubular tubular JJ cord-022774-wasdp2gh 10 17 elements element NNS cord-022774-wasdp2gh 10 18 accumulated accumulate VBN cord-022774-wasdp2gh 10 19 . . . cord-022774-wasdp2gh 11 1 Those those DT cord-022774-wasdp2gh 11 2 transition transition NN cord-022774-wasdp2gh 11 3 vesicles vesicle NNS cord-022774-wasdp2gh 11 4 which which WDT cord-022774-wasdp2gh 11 5 formed form VBD cord-022774-wasdp2gh 11 6 at at IN cord-022774-wasdp2gh 11 7 37 37 CD cord-022774-wasdp2gh 11 8 ° ° NN cord-022774-wasdp2gh 11 9 C C NNP cord-022774-wasdp2gh 11 10 were be VBD cord-022774-wasdp2gh 11 11 of of IN cord-022774-wasdp2gh 11 12 a a DT cord-022774-wasdp2gh 11 13 greater great JJR cord-022774-wasdp2gh 11 14 diameter diameter NN cord-022774-wasdp2gh 11 15 than than IN cord-022774-wasdp2gh 11 16 those those DT cord-022774-wasdp2gh 11 17 formed form VBN cord-022774-wasdp2gh 11 18 at at IN cord-022774-wasdp2gh 11 19 4 4 CD cord-022774-wasdp2gh 11 20 ° ° NN cord-022774-wasdp2gh 11 21 C c NN cord-022774-wasdp2gh 11 22 both both CC cord-022774-wasdp2gh 11 23 in in IN cord-022774-wasdp2gh 11 24 situ situ NN cord-022774-wasdp2gh 11 25 and and CC cord-022774-wasdp2gh 11 26 in in IN cord-022774-wasdp2gh 11 27 vitro vitro FW cord-022774-wasdp2gh 11 28 . . . cord-022774-wasdp2gh 12 1 The the DT cord-022774-wasdp2gh 12 2 findings finding NNS cord-022774-wasdp2gh 12 3 show show VBP cord-022774-wasdp2gh 12 4 parallel parallel JJ cord-022774-wasdp2gh 12 5 responses response NNS cord-022774-wasdp2gh 12 6 between between IN cord-022774-wasdp2gh 12 7 the the DT cord-022774-wasdp2gh 12 8 temperature temperature NN cord-022774-wasdp2gh 12 9 dependency dependency NN cord-022774-wasdp2gh 12 10 of of IN cord-022774-wasdp2gh 12 11 transition transition NN cord-022774-wasdp2gh 12 12 vesicle vesicle NN cord-022774-wasdp2gh 12 13 formation formation NN cord-022774-wasdp2gh 12 14 in in IN cord-022774-wasdp2gh 12 15 vitro vitro FW cord-022774-wasdp2gh 12 16 and and CC cord-022774-wasdp2gh 12 17 in in IN cord-022774-wasdp2gh 12 18 situ situ NN cord-022774-wasdp2gh 12 19 and and CC cord-022774-wasdp2gh 12 20 suggest suggest VBP cord-022774-wasdp2gh 12 21 that that IN cord-022774-wasdp2gh 12 22 a a DT cord-022774-wasdp2gh 12 23 subpopulation subpopulation NN cord-022774-wasdp2gh 12 24 of of IN cord-022774-wasdp2gh 12 25 the the DT cord-022774-wasdp2gh 12 26 transitional transitional JJ cord-022774-wasdp2gh 12 27 endoplasmic endoplasmic NN cord-022774-wasdp2gh 12 28 reticulum reticulum NN cord-022774-wasdp2gh 12 29 may may MD cord-022774-wasdp2gh 12 30 be be VB cord-022774-wasdp2gh 12 31 morphologically morphologically RB cord-022774-wasdp2gh 12 32 and and CC cord-022774-wasdp2gh 12 33 functionally functionally RB cord-022774-wasdp2gh 12 34 homologous homologous JJ cord-022774-wasdp2gh 12 35 to to IN cord-022774-wasdp2gh 12 36 the the DT cord-022774-wasdp2gh 12 37 16 16 CD cord-022774-wasdp2gh 12 38 ° ° NN cord-022774-wasdp2gh 12 39 C C NNP cord-022774-wasdp2gh 12 40 compartment compartment NN cord-022774-wasdp2gh 12 41 observed observe VBN cord-022774-wasdp2gh 12 42 in in IN cord-022774-wasdp2gh 12 43 virally‐infected virally‐infected JJ cord-022774-wasdp2gh 12 44 cell cell NN cord-022774-wasdp2gh 12 45 lines line NNS cord-022774-wasdp2gh 12 46 grown grow VBN cord-022774-wasdp2gh 12 47 at at IN cord-022774-wasdp2gh 12 48 low low JJ cord-022774-wasdp2gh 12 49 temperatures temperature NNS cord-022774-wasdp2gh 12 50 . . . cord-022774-wasdp2gh 13 1 Reduced reduce VBN cord-022774-wasdp2gh 13 2 temperature temperature NN cord-022774-wasdp2gh 13 3 has have VBZ cord-022774-wasdp2gh 13 4 been be VBN cord-022774-wasdp2gh 13 5 shown show VBN cord-022774-wasdp2gh 13 6 to to TO cord-022774-wasdp2gh 13 7 affect affect VB cord-022774-wasdp2gh 13 8 posttranslational posttranslational JJ cord-022774-wasdp2gh 13 9 processing processing NN cord-022774-wasdp2gh 13 10 and and CC cord-022774-wasdp2gh 13 11 secretion secretion NN cord-022774-wasdp2gh 13 12 in in IN cord-022774-wasdp2gh 13 13 a a DT cord-022774-wasdp2gh 13 14 number number NN cord-022774-wasdp2gh 13 15 of of IN cord-022774-wasdp2gh 13 16 cell cell NN cord-022774-wasdp2gh 13 17 systems system NNS cord-022774-wasdp2gh 13 18 . . . cord-022774-wasdp2gh 14 1 Tartakoff Tartakoff NNP cord-022774-wasdp2gh 14 2 [ [ -LRB- cord-022774-wasdp2gh 14 3 19 19 CD cord-022774-wasdp2gh 14 4 ] ] -RRB- cord-022774-wasdp2gh 14 5 demonstrated demonstrate VBD cord-022774-wasdp2gh 14 6 that that IN cord-022774-wasdp2gh 14 7 at at IN cord-022774-wasdp2gh 14 8 10 10 CD cord-022774-wasdp2gh 14 9 ° ° NN cord-022774-wasdp2gh 14 10 C c NN cord-022774-wasdp2gh 14 11 secretory secretory NN cord-022774-wasdp2gh 14 12 proteins protein NNS cord-022774-wasdp2gh 14 13 of of IN cord-022774-wasdp2gh 14 14 the the DT cord-022774-wasdp2gh 14 15 exocrine exocrine JJ cord-022774-wasdp2gh 14 16 pancreas pancreas NN cord-022774-wasdp2gh 14 17 accumulated accumulate VBN cord-022774-wasdp2gh 14 18 in in IN cord-022774-wasdp2gh 14 19 pre pre JJ cord-022774-wasdp2gh 14 20 - - JJ cord-022774-wasdp2gh 14 21 Golgi golgi NN cord-022774-wasdp2gh 14 22 apparatus apparatus NN cord-022774-wasdp2gh 14 23 transition transition NN cord-022774-wasdp2gh 14 24 vesicles vesicle NNS cord-022774-wasdp2gh 14 25 , , , cord-022774-wasdp2gh 14 26 while while IN cord-022774-wasdp2gh 14 27 at at IN cord-022774-wasdp2gh 14 28 temperatures temperature NNS cord-022774-wasdp2gh 14 29 of of IN cord-022774-wasdp2gh 14 30 22 22 CD cord-022774-wasdp2gh 14 31 ° ° NN cord-022774-wasdp2gh 14 32 C c NN cord-022774-wasdp2gh 14 33 or or CC cord-022774-wasdp2gh 14 34 greater great JJR cord-022774-wasdp2gh 14 35 , , , cord-022774-wasdp2gh 14 36 progress progress VB cord-022774-wasdp2gh 14 37 through through IN cord-022774-wasdp2gh 14 38 the the DT cord-022774-wasdp2gh 14 39 Golgi Golgi NNP cord-022774-wasdp2gh 14 40 apparatus apparatus NN cord-022774-wasdp2gh 14 41 and and CC cord-022774-wasdp2gh 14 42 into into IN cord-022774-wasdp2gh 14 43 condensing condense VBG cord-022774-wasdp2gh 14 44 vacuoles vacuole NNS cord-022774-wasdp2gh 14 45 occurred occur VBD cord-022774-wasdp2gh 14 46 . . . cord-022774-wasdp2gh 15 1 Secretion secretion NN cord-022774-wasdp2gh 15 2 of of IN cord-022774-wasdp2gh 15 3 IgM IgM NNP cord-022774-wasdp2gh 15 4 and and CC cord-022774-wasdp2gh 15 5 the the DT cord-022774-wasdp2gh 15 6 appearance appearance NN cord-022774-wasdp2gh 15 7 of of IN cord-022774-wasdp2gh 15 8 HLA-1 HLA-1 NNP cord-022774-wasdp2gh 15 9 on on IN cord-022774-wasdp2gh 15 10 the the DT cord-022774-wasdp2gh 15 11 cell cell NN cord-022774-wasdp2gh 15 12 surface surface NN cord-022774-wasdp2gh 15 13 decreased decrease VBD cord-022774-wasdp2gh 15 14 gradually gradually RB cord-022774-wasdp2gh 15 15 in in IN cord-022774-wasdp2gh 15 16 lymphoid lymphoid JJ cord-022774-wasdp2gh 15 17 cells cell NNS cord-022774-wasdp2gh 15 18 as as IN cord-022774-wasdp2gh 15 19 the the DT cord-022774-wasdp2gh 15 20 temperature temperature NN cord-022774-wasdp2gh 15 21 was be VBD cord-022774-wasdp2gh 15 22 reduced reduce VBN cord-022774-wasdp2gh 15 23 from from IN cord-022774-wasdp2gh 15 24 37 37 CD cord-022774-wasdp2gh 15 25 ° ° NN cord-022774-wasdp2gh 15 26 C c NN cord-022774-wasdp2gh 15 27 to to IN cord-022774-wasdp2gh 15 28 20 20 CD cord-022774-wasdp2gh 15 29 ° ° NN cord-022774-wasdp2gh 15 30 C c NN cord-022774-wasdp2gh 15 31 but but CC cord-022774-wasdp2gh 15 32 no no DT cord-022774-wasdp2gh 15 33 intracellular intracellular JJ cord-022774-wasdp2gh 15 34 accumulation accumulation NN cord-022774-wasdp2gh 15 35 was be VBD cord-022774-wasdp2gh 15 36 observed observe VBN cord-022774-wasdp2gh 15 37 [ [ -LRB- cord-022774-wasdp2gh 15 38 3 3 CD cord-022774-wasdp2gh 15 39 ] ] -RRB- cord-022774-wasdp2gh 15 40 . . . cord-022774-wasdp2gh 16 1 Histamine histamine NN cord-022774-wasdp2gh 16 2 secretion secretion NN cord-022774-wasdp2gh 16 3 from from IN cord-022774-wasdp2gh 16 4 mast mast NN cord-022774-wasdp2gh 16 5 cells cell NNS cord-022774-wasdp2gh 16 6 also also RB cord-022774-wasdp2gh 16 7 decreased decrease VBD cord-022774-wasdp2gh 16 8 markedly markedly RB cord-022774-wasdp2gh 16 9 at at IN cord-022774-wasdp2gh 16 10 temperatures temperature NNS cord-022774-wasdp2gh 16 11 below below IN cord-022774-wasdp2gh 16 12 18 18 CD cord-022774-wasdp2gh 16 13 ° ° NN cord-022774-wasdp2gh 16 14 C c NN cord-022774-wasdp2gh 16 15 [ [ -LRB- cord-022774-wasdp2gh 16 16 10 10 CD cord-022774-wasdp2gh 16 17 ] ] -RRB- cord-022774-wasdp2gh 16 18 . . . cord-022774-wasdp2gh 17 1 With with IN cord-022774-wasdp2gh 17 2 hepatic hepatic JJ cord-022774-wasdp2gh 17 3 cells cell NNS cord-022774-wasdp2gh 17 4 , , , cord-022774-wasdp2gh 17 5 secretory secretory NN cord-022774-wasdp2gh 17 6 p p NN cord-022774-wasdp2gh 17 7 ~ ~ CD cord-022774-wasdp2gh 17 8 oteins otein NNS cord-022774-wasdp2gh 17 9 were be VBD cord-022774-wasdp2gh 17 10 blocked block VBN cord-022774-wasdp2gh 17 11 in in IN cord-022774-wasdp2gh 17 12 a a DT cord-022774-wasdp2gh 17 13 pre pre JJ cord-022774-wasdp2gh 17 14 - - JJ cord-022774-wasdp2gh 17 15 Golgi golgi NN cord-022774-wasdp2gh 17 16 apparatus apparatus NN cord-022774-wasdp2gh 17 17 compartment compartment NN cord-022774-wasdp2gh 17 18 at at IN cord-022774-wasdp2gh 17 19 18 18 CD cord-022774-wasdp2gh 17 20 ° ° NN cord-022774-wasdp2gh 17 21 C c NN cord-022774-wasdp2gh 17 22 , , , cord-022774-wasdp2gh 17 23 but but CC cord-022774-wasdp2gh 17 24 at at IN cord-022774-wasdp2gh 17 25 20 20 CD cord-022774-wasdp2gh 17 26 ° ° NN cord-022774-wasdp2gh 17 27 C c NN cord-022774-wasdp2gh 17 28 these these DT cord-022774-wasdp2gh 17 29 proteins protein NNS cord-022774-wasdp2gh 17 30 were be VBD cord-022774-wasdp2gh 17 31 exocytosed exocytose VBN cord-022774-wasdp2gh 17 32 normally normally RB cord-022774-wasdp2gh 17 33 [ [ -LRB- cord-022774-wasdp2gh 17 34 5 5 CD cord-022774-wasdp2gh 17 35 ] ] -RRB- cord-022774-wasdp2gh 17 36 . . . cord-022774-wasdp2gh 18 1 This this DT cord-022774-wasdp2gh 18 2 was be VBD cord-022774-wasdp2gh 18 3 similar similar JJ cord-022774-wasdp2gh 18 4 to to IN cord-022774-wasdp2gh 18 5 rat rat NN cord-022774-wasdp2gh 18 6 pancreatic pancreatic JJ cord-022774-wasdp2gh 18 7 cells cell NNS cord-022774-wasdp2gh 18 8 where where WRB cord-022774-wasdp2gh 18 9 , , , cord-022774-wasdp2gh 18 10 at at IN cord-022774-wasdp2gh 18 11 16 16 CD cord-022774-wasdp2gh 18 12 ° ° NN cord-022774-wasdp2gh 18 13 C c NN cord-022774-wasdp2gh 18 14 , , , cord-022774-wasdp2gh 18 15 most most JJS cord-022774-wasdp2gh 18 16 of of IN cord-022774-wasdp2gh 18 17 the the DT cord-022774-wasdp2gh 18 18 • • NNP cord-022774-wasdp2gh 18 19 Correspondence Correspondence NNP cord-022774-wasdp2gh 18 20 and and CC cord-022774-wasdp2gh 18 21 reprints reprint NNS cord-022774-wasdp2gh 18 22 . . . cord-022774-wasdp2gh 19 1 This this DT cord-022774-wasdp2gh 19 2 article article NN cord-022774-wasdp2gh 19 3 is be VBZ cord-022774-wasdp2gh 19 4 dedicated dedicate VBN cord-022774-wasdp2gh 19 5 to to IN cord-022774-wasdp2gh 19 6 the the DT cord-022774-wasdp2gh 19 7 memory memory NN cord-022774-wasdp2gh 19 8 of of IN cord-022774-wasdp2gh 19 9 Professor Professor NNP cord-022774-wasdp2gh 19 10 Pierre Pierre NNP cord-022774-wasdp2gh 19 11 Favard Favard NNP cord-022774-wasdp2gh 19 12 . . . cord-022774-wasdp2gh 20 1 labeled label VBN cord-022774-wasdp2gh 20 2 secretory secretory JJ cord-022774-wasdp2gh 20 3 proteins protein NNS cord-022774-wasdp2gh 20 4 remained remain VBD cord-022774-wasdp2gh 20 5 in in IN cord-022774-wasdp2gh 20 6 the the DT cord-022774-wasdp2gh 20 7 endoplasmic endoplasmic NN cord-022774-wasdp2gh 20 8 reticulum reticulum NN cord-022774-wasdp2gh 20 9 while while IN cord-022774-wasdp2gh 20 10 at at IN cord-022774-wasdp2gh 20 11 20 20 CD cord-022774-wasdp2gh 20 12 ° ° NN cord-022774-wasdp2gh 20 13 C C NNP cord-022774-wasdp2gh 20 14 the the DT cord-022774-wasdp2gh 20 15 medial medial JJ cord-022774-wasdp2gh 20 16 Golgi Golgi NNP cord-022774-wasdp2gh 20 17 region region NN cord-022774-wasdp2gh 20 18 was be VBD cord-022774-wasdp2gh 20 19 reached reach VBN cord-022774-wasdp2gh 20 20 [ [ -LRB- cord-022774-wasdp2gh 20 21 18 18 CD cord-022774-wasdp2gh 20 22 ] ] -RRB- cord-022774-wasdp2gh 20 23 . . . cord-022774-wasdp2gh 21 1 These these DT cord-022774-wasdp2gh 21 2 and and CC cord-022774-wasdp2gh 21 3 other other JJ cord-022774-wasdp2gh 21 4 data datum NNS cord-022774-wasdp2gh 21 5 , , , cord-022774-wasdp2gh 21 6 especially especially RB cord-022774-wasdp2gh 21 7 with with IN cord-022774-wasdp2gh 21 8 the the DT cord-022774-wasdp2gh 21 9 intracellular intracellular JJ cord-022774-wasdp2gh 21 10 transport transport NN cord-022774-wasdp2gh 21 11 and and CC cord-022774-wasdp2gh 21 12 surface surface NN cord-022774-wasdp2gh 21 13 expression expression NN cord-022774-wasdp2gh 21 14 of of IN cord-022774-wasdp2gh 21 15 protein protein NN cord-022774-wasdp2gh 21 16 in in IN cord-022774-wasdp2gh 21 17 virusinfected virusinfected JJ cord-022774-wasdp2gh 21 18 cells cell NNS cord-022774-wasdp2gh 21 19 H H NNP cord-022774-wasdp2gh 21 20 , , , cord-022774-wasdp2gh 21 21 6 6 CD cord-022774-wasdp2gh 21 22 , , , cord-022774-wasdp2gh 21 23 8 8 CD cord-022774-wasdp2gh 21 24 , , , cord-022774-wasdp2gh 21 25 12 12 CD cord-022774-wasdp2gh 21 26 , , , cord-022774-wasdp2gh 21 27 17 17 CD cord-022774-wasdp2gh 21 28 , , , cord-022774-wasdp2gh 21 29 20 20 CD cord-022774-wasdp2gh 21 30 , , , cord-022774-wasdp2gh 21 31 21 21 CD cord-022774-wasdp2gh 21 32 ] ] -RRB- cord-022774-wasdp2gh 21 33 has have VBZ cord-022774-wasdp2gh 21 34 led lead VBN cord-022774-wasdp2gh 21 35 to to IN cord-022774-wasdp2gh 21 36 the the DT cord-022774-wasdp2gh 21 37 concept concept NN cord-022774-wasdp2gh 21 38 of of IN cord-022774-wasdp2gh 21 39 a a DT cord-022774-wasdp2gh 21 40 16 16 CD cord-022774-wasdp2gh 21 41 ° ° NN cord-022774-wasdp2gh 21 42 C c NN cord-022774-wasdp2gh 21 43 compartment compartment NN cord-022774-wasdp2gh 21 44 . . . cord-022774-wasdp2gh 22 1 This this DT cord-022774-wasdp2gh 22 2 pre pre JJ cord-022774-wasdp2gh 22 3 - - JJ cord-022774-wasdp2gh 22 4 Golgi golgi NN cord-022774-wasdp2gh 22 5 apparatus apparatus NN cord-022774-wasdp2gh 22 6 compartment compartment NN cord-022774-wasdp2gh 22 7 would would MD cord-022774-wasdp2gh 22 8 differ differ VB cord-022774-wasdp2gh 22 9 from from IN cord-022774-wasdp2gh 22 10 the the DT cord-022774-wasdp2gh 22 11 bulk bulk NN cord-022774-wasdp2gh 22 12 of of IN cord-022774-wasdp2gh 22 13 the the DT cord-022774-wasdp2gh 22 14 endoplasmic endoplasmic NN cord-022774-wasdp2gh 22 15 reticulum reticulum NN cord-022774-wasdp2gh 22 16 and and CC cord-022774-wasdp2gh 22 17 would would MD cord-022774-wasdp2gh 22 18 presumably presumably RB cord-022774-wasdp2gh 22 19 be be VB cord-022774-wasdp2gh 22 20 located locate VBN cord-022774-wasdp2gh 22 21 in in IN cord-022774-wasdp2gh 22 22 the the DT cord-022774-wasdp2gh 22 23 secretory secretory JJ cord-022774-wasdp2gh 22 24 pathway pathway NN cord-022774-wasdp2gh 22 25 between between IN cord-022774-wasdp2gh 22 26 the the DT cord-022774-wasdp2gh 22 27 transitional transitional JJ cord-022774-wasdp2gh 22 28 endoplasmic endoplasmic NN cord-022774-wasdp2gh 22 29 reticulum reticulum NN cord-022774-wasdp2gh 22 30 and and CC cord-022774-wasdp2gh 22 31 the the DT cord-022774-wasdp2gh 22 32 Golgi Golgi NNP cord-022774-wasdp2gh 22 33 apparatus apparatus NN cord-022774-wasdp2gh 22 34 . . . cord-022774-wasdp2gh 23 1 Despite despite IN cord-022774-wasdp2gh 23 2 the the DT cord-022774-wasdp2gh 23 3 considerable considerable JJ cord-022774-wasdp2gh 23 4 evidence evidence NN cord-022774-wasdp2gh 23 5 for for IN cord-022774-wasdp2gh 23 6 the the DT cord-022774-wasdp2gh 23 7 existence existence NN cord-022774-wasdp2gh 23 8 of of IN cord-022774-wasdp2gh 23 9 such such PDT cord-022774-wasdp2gh 23 10 a a DT cord-022774-wasdp2gh 23 11 compartment compartment NN cord-022774-wasdp2gh 23 12 based base VBN cord-022774-wasdp2gh 23 13 on on IN cord-022774-wasdp2gh 23 14 the the DT cord-022774-wasdp2gh 23 15 interruption interruption NN cord-022774-wasdp2gh 23 16 of of IN cord-022774-wasdp2gh 23 17 processing processing NN cord-022774-wasdp2gh 23 18 and and CC cord-022774-wasdp2gh 23 19 transport transport NN cord-022774-wasdp2gh 23 20 of of IN cord-022774-wasdp2gh 23 21 secretory secretory NN cord-022774-wasdp2gh 23 22 and and CC cord-022774-wasdp2gh 23 23 membrane membrane NN cord-022774-wasdp2gh 23 24 proteins protein NNS cord-022774-wasdp2gh 23 25 , , , cord-022774-wasdp2gh 23 26 the the DT cord-022774-wasdp2gh 23 27 data datum NNS cord-022774-wasdp2gh 23 28 on on IN cord-022774-wasdp2gh 23 29 the the DT cord-022774-wasdp2gh 23 30 morphological morphological JJ cord-022774-wasdp2gh 23 31 identification identification NN cord-022774-wasdp2gh 23 32 of of IN cord-022774-wasdp2gh 23 33 such such PDT cord-022774-wasdp2gh 23 34 an an DT cord-022774-wasdp2gh 23 35 intermediate intermediate JJ cord-022774-wasdp2gh 23 36 compartment compartment NN cord-022774-wasdp2gh 23 37 are be VBP cord-022774-wasdp2gh 23 38 more more RBR cord-022774-wasdp2gh 23 39 limited limited JJ cord-022774-wasdp2gh 23 40 and and CC cord-022774-wasdp2gh 23 41 somewhat somewhat RB cord-022774-wasdp2gh 23 42 conflicting conflicting JJ cord-022774-wasdp2gh 23 43 . . . cord-022774-wasdp2gh 24 1 This this DT cord-022774-wasdp2gh 24 2 study study NN cord-022774-wasdp2gh 24 3 was be VBD cord-022774-wasdp2gh 24 4 undertaken undertake VBN cord-022774-wasdp2gh 24 5 to to TO cord-022774-wasdp2gh 24 6 utilize utilize VB cord-022774-wasdp2gh 24 7 quantitative quantitative JJ cord-022774-wasdp2gh 24 8 morphometric morphometric JJ cord-022774-wasdp2gh 24 9 methods method NNS cord-022774-wasdp2gh 24 10 to to TO cord-022774-wasdp2gh 24 11 identify identify VB cord-022774-wasdp2gh 24 12 that that DT cord-022774-wasdp2gh 24 13 endomembrane endomembrane NNP cord-022774-wasdp2gh 24 14 compartment compartment NN cord-022774-wasdp2gh 24 15 of of IN cord-022774-wasdp2gh 24 16 . . . cord-022774-wasdp2gh 25 1 rat rat NN cord-022774-wasdp2gh 25 2 liver liver NN cord-022774-wasdp2gh 25 3 which which WDT cord-022774-wasdp2gh 25 4 may may MD cord-022774-wasdp2gh 25 5 accumulate accumulate VB cord-022774-wasdp2gh 25 6 at at IN cord-022774-wasdp2gh 25 7 temperatures temperature NNS cord-022774-wasdp2gh 25 8 below below IN cord-022774-wasdp2gh 25 9 18 18 CD cord-022774-wasdp2gh 25 10 ° ° NN cord-022774-wasdp2gh 25 11 C c NN cord-022774-wasdp2gh 25 12 and and CC cord-022774-wasdp2gh 25 13 in in IN cord-022774-wasdp2gh 25 14 cells cell NNS cord-022774-wasdp2gh 25 15 in in IN cord-022774-wasdp2gh 25 16 culture culture NN cord-022774-wasdp2gh 25 17 for for IN cord-022774-wasdp2gh 25 18 comparison comparison NN cord-022774-wasdp2gh 25 19 . . . cord-022774-wasdp2gh 26 1 Studies study NNS cord-022774-wasdp2gh 26 2 were be VBD cord-022774-wasdp2gh 26 3 then then RB cord-022774-wasdp2gh 26 4 conducted conduct VBN cord-022774-wasdp2gh 26 5 with with IN cord-022774-wasdp2gh 26 6 isolated isolate VBN cord-022774-wasdp2gh 26 7 transitional transitional JJ cord-022774-wasdp2gh 26 8 endoplasmic endoplasmic NN cord-022774-wasdp2gh 26 9 reticulum reticulum NN cord-022774-wasdp2gh 26 10 fractions fraction NNS cord-022774-wasdp2gh 26 11 from from IN cord-022774-wasdp2gh 26 12 rat rat NN cord-022774-wasdp2gh 26 13 liver liver NN cord-022774-wasdp2gh 26 14 to to TO cord-022774-wasdp2gh 26 15 determine determine VB cord-022774-wasdp2gh 26 16 if if IN cord-022774-wasdp2gh 26 17 a a DT cord-022774-wasdp2gh 26 18 similar similar JJ cord-022774-wasdp2gh 26 19 mechanism mechanism NN cord-022774-wasdp2gh 26 20 of of IN cord-022774-wasdp2gh 26 21 control control NN cord-022774-wasdp2gh 26 22 by by IN cord-022774-wasdp2gh 26 23 temperature temperature NN cord-022774-wasdp2gh 26 24 of of IN cord-022774-wasdp2gh 26 25 transition transition NN cord-022774-wasdp2gh 26 26 vesicle vesicle NN cord-022774-wasdp2gh 26 27 formation formation NN cord-022774-wasdp2gh 26 28 could could MD cord-022774-wasdp2gh 26 29 be be VB cord-022774-wasdp2gh 26 30 duplicated duplicate VBN cord-022774-wasdp2gh 26 31 in in IN cord-022774-wasdp2gh 26 32 the the DT cord-022774-wasdp2gh 26 33 cell cell NN cord-022774-wasdp2gh 26 34 - - HYPH cord-022774-wasdp2gh 26 35 free free JJ cord-022774-wasdp2gh 26 36 system system NN cord-022774-wasdp2gh 26 37 . . . cord-022774-wasdp2gh 27 1 Male male JJ cord-022774-wasdp2gh 27 2 Holtzman Holtzman NNP cord-022774-wasdp2gh 27 3 rats rat NNS cord-022774-wasdp2gh 27 4 were be VBD cord-022774-wasdp2gh 27 5 sacrificed sacrifice VBN cord-022774-wasdp2gh 27 6 and and CC cord-022774-wasdp2gh 27 7 livers liver NNS cord-022774-wasdp2gh 27 8 removed remove VBN cord-022774-wasdp2gh 27 9 . . . cord-022774-wasdp2gh 28 1 Unless unless IN cord-022774-wasdp2gh 28 2 stated state VBN cord-022774-wasdp2gh 28 3 otherwise otherwise RB cord-022774-wasdp2gh 28 4 , , , cord-022774-wasdp2gh 28 5 slices slice NNS cord-022774-wasdp2gh 28 6 of of IN cord-022774-wasdp2gh 28 7 liver liver NN cord-022774-wasdp2gh 28 8 averaging average VBG cord-022774-wasdp2gh 28 9 about about IN cord-022774-wasdp2gh 28 10 1.5 1.5 CD cord-022774-wasdp2gh 28 11 x x SYM cord-022774-wasdp2gh 28 12 0.3 0.3 CD cord-022774-wasdp2gh 28 13 x x SYM cord-022774-wasdp2gh 28 14 0.3 0.3 CD cord-022774-wasdp2gh 28 15 mm mm NNS cord-022774-wasdp2gh 28 16 were be VBD cord-022774-wasdp2gh 28 17 cut cut VBN cord-022774-wasdp2gh 28 18 by by IN cord-022774-wasdp2gh 28 19 hand hand NN cord-022774-wasdp2gh 28 20 with with IN cord-022774-wasdp2gh 28 21 a a DT cord-022774-wasdp2gh 28 22 razor razor NN cord-022774-wasdp2gh 28 23 blade blade NN cord-022774-wasdp2gh 28 24 as as IN cord-022774-wasdp2gh 28 25 described describe VBN cord-022774-wasdp2gh 28 26 [ [ -LRB- cord-022774-wasdp2gh 28 27 14 14 CD cord-022774-wasdp2gh 28 28 ] ] -RRB- cord-022774-wasdp2gh 28 29 . . . cord-022774-wasdp2gh 29 1 Slices slice NNS cord-022774-wasdp2gh 29 2 were be VBD cord-022774-wasdp2gh 29 3 incubated incubate VBN cord-022774-wasdp2gh 29 4 in in IN cord-022774-wasdp2gh 29 5 phosphate phosphate NN cord-022774-wasdp2gh 29 6 - - HYPH cord-022774-wasdp2gh 29 7 buffered buffer VBN cord-022774-wasdp2gh 29 8 saline saline NN cord-022774-wasdp2gh 29 9 at at IN cord-022774-wasdp2gh 29 10 the the DT cord-022774-wasdp2gh 29 11 temperatures temperature NNS cord-022774-wasdp2gh 29 12 indicated indicate VBD cord-022774-wasdp2gh 29 13 for for IN cord-022774-wasdp2gh 29 14 30 30 CD cord-022774-wasdp2gh 29 15 or or CC cord-022774-wasdp2gh 29 16 60 60 CD cord-022774-wasdp2gh 29 17 rain rain NN cord-022774-wasdp2gh 29 18 and and CC cord-022774-wasdp2gh 29 19 fixed fix VBN cord-022774-wasdp2gh 29 20 directly directly RB cord-022774-wasdp2gh 29 21 with with IN cord-022774-wasdp2gh 29 22 1 1 CD cord-022774-wasdp2gh 29 23 070 070 CD cord-022774-wasdp2gh 29 24 osmium osmium NN cord-022774-wasdp2gh 29 25 tetroxide tetroxide NN cord-022774-wasdp2gh 29 26 . . . cord-022774-wasdp2gh 30 1 Cell cell NN cord-022774-wasdp2gh 30 2 culture culture NN cord-022774-wasdp2gh 31 1 BHK BHK NNP cord-022774-wasdp2gh 32 1 ( ( -LRB- cord-022774-wasdp2gh 32 2 baby baby NN cord-022774-wasdp2gh 32 3 hamster hamster NN cord-022774-wasdp2gh 32 4 kidney kidney NN cord-022774-wasdp2gh 32 5 ) ) -RRB- cord-022774-wasdp2gh 32 6 cells cell NNS cord-022774-wasdp2gh 32 7 were be VBD cord-022774-wasdp2gh 32 8 grown grow VBN cord-022774-wasdp2gh 32 9 in in IN cord-022774-wasdp2gh 32 10 MEM MEM NNP cord-022774-wasdp2gh 33 1 ( ( -LRB- cord-022774-wasdp2gh 33 2 Eagles Eagles NNP cord-022774-wasdp2gh 33 3 Minimum Minimum NNP cord-022774-wasdp2gh 33 4 Essential Essential NNP cord-022774-wasdp2gh 33 5 Medium medium NN cord-022774-wasdp2gh 33 6 ) ) -RRB- cord-022774-wasdp2gh 34 1 supplc;,~ented supplc;,~ente VBN cord-022774-wasdp2gh 34 2 with with IN cord-022774-wasdp2gh 34 3 7070 7070 CD cord-022774-wasdp2gh 34 4 fetal fetal JJ cord-022774-wasdp2gh 34 5 calf calf NN cord-022774-wasdp2gh 34 6 serum serum NN cord-022774-wasdp2gh 34 7 , , , cord-022774-wasdp2gh 34 8 10 10 CD cord-022774-wasdp2gh 34 9 ° ° NN cord-022774-wasdp2gh 34 10 70 70 CD cord-022774-wasdp2gh 34 11 triptose triptose NN cord-022774-wasdp2gh 34 12 phosphate phosphate NN cord-022774-wasdp2gh 34 13 , , , cord-022774-wasdp2gh 34 14 100 100 CD cord-022774-wasdp2gh 34 15 U u NN cord-022774-wasdp2gh 34 16 / / SYM cord-022774-wasdp2gh 34 17 ml ml NN cord-022774-wasdp2gh 34 18 penicillin penicillin NN cord-022774-wasdp2gh 34 19 and and CC cord-022774-wasdp2gh 34 20 100 100 CD cord-022774-wasdp2gh 34 21 tz~/mi tz~/mi XX cord-022774-wasdp2gh 34 22 streptomycin streptomycin NNS cord-022774-wasdp2gh 34 23 [ [ -LRB- cord-022774-wasdp2gh 34 24 221 221 CD cord-022774-wasdp2gh 34 25 . . . cord-022774-wasdp2gh 35 1 All all DT cord-022774-wasdp2gh 35 2 temperature temperature NN cord-022774-wasdp2gh 35 3 experiments experiment NNS cord-022774-wasdp2gh 35 4 were be VBD cord-022774-wasdp2gh 35 5 carried carry VBN cord-022774-wasdp2gh 35 6 out out RP cord-022774-wasdp2gh 35 7 in in IN cord-022774-wasdp2gh 35 8 constant constant JJ cord-022774-wasdp2gh 35 9 temperature temperature NN cord-022774-wasdp2gh 35 10 incubators incubator NNS cord-022774-wasdp2gh 35 11 ( ( -LRB- cord-022774-wasdp2gh 35 12 ± ± CD cord-022774-wasdp2gh 35 13 0.50C 0.50c CD cord-022774-wasdp2gh 35 14 ) ) -RRB- cord-022774-wasdp2gh 35 15 . . . cord-022774-wasdp2gh 36 1 The the DT cord-022774-wasdp2gh 36 2 incubators incubator NNS cord-022774-wasdp2gh 36 3 were be VBD cord-022774-wasdp2gh 36 4 housed house VBN cord-022774-wasdp2gh 36 5 in in IN cord-022774-wasdp2gh 36 6 a a DT cord-022774-wasdp2gh 36 7 refrigerated refrigerated JJ cord-022774-wasdp2gh 36 8 room room NN cord-022774-wasdp2gh 36 9 ( ( -LRB- cord-022774-wasdp2gh 36 10 4"C 4"c CD cord-022774-wasdp2gh 36 11 ) ) -RRB- cord-022774-wasdp2gh 36 12 to to TO cord-022774-wasdp2gh 36 13 ensure ensure VB cord-022774-wasdp2gh 36 14 that that IN cord-022774-wasdp2gh 36 15 the the DT cord-022774-wasdp2gh 36 16 room room NN cord-022774-wasdp2gh 36 17 temperature temperature NN cord-022774-wasdp2gh 36 18 never never RB cord-022774-wasdp2gh 36 19 exceeded exceed VBD cord-022774-wasdp2gh 36 20 that that DT cord-022774-wasdp2gh 36 21 of of IN cord-022774-wasdp2gh 36 22 the the DT cord-022774-wasdp2gh 36 23 chambers chamber NNS cord-022774-wasdp2gh 36 24 . . . cord-022774-wasdp2gh 37 1 Equilibration equilibration NN cord-022774-wasdp2gh 37 2 of of IN cord-022774-wasdp2gh 37 3 culture culture NN cord-022774-wasdp2gh 37 4 media medium NNS cord-022774-wasdp2gh 37 5 , , , cord-022774-wasdp2gh 37 6 membrane membrane NN cord-022774-wasdp2gh 37 7 preparatinn preparatinn NN cord-022774-wasdp2gh 37 8 and and CC cord-022774-wasdp2gh 37 9 liver liver NN cord-022774-wasdp2gh 37 10 slices slice NNS cord-022774-wasdp2gh 37 11 to to IN cord-022774-wasdp2gh 37 12 the the DT cord-022774-wasdp2gh 37 13 temperature temperature NN cord-022774-wasdp2gh 37 14 of of IN cord-022774-wasdp2gh 37 15 the the DT cord-022774-wasdp2gh 37 16 chambers chamber NNS cord-022774-wasdp2gh 37 17 occurred occur VBD cord-022774-wasdp2gh 37 18 rapidly rapidly RB cord-022774-wasdp2gh 37 19 in in IN cord-022774-wasdp2gh 37 20 a a DT cord-022774-wasdp2gh 37 21 matter matter NN cord-022774-wasdp2gh 37 22 of of IN cord-022774-wasdp2gh 37 23 a a DT cord-022774-wasdp2gh 37 24 few few JJ cord-022774-wasdp2gh 37 25 minutes minute NNS cord-022774-wasdp2gh 37 26 . . . cord-022774-wasdp2gh 38 1 Fixations fixation NNS cord-022774-wasdp2gh 38 2 were be VBD cord-022774-wasdp2gh 38 3 at at IN cord-022774-wasdp2gh 38 4 the the DT cord-022774-wasdp2gh 38 5 same same JJ cord-022774-wasdp2gh 38 6 temperature temperature NN cord-022774-wasdp2gh 38 7 as as IN cord-022774-wasdp2gh 38 8 the the DT cord-022774-wasdp2gh 38 9 incubations incubation NNS cord-022774-wasdp2gh 38 10 . . . cord-022774-wasdp2gh 39 1 Cells cell NNS cord-022774-wasdp2gh 39 2 or or CC cord-022774-wasdp2gh 39 3 isolated isolate VBN cord-022774-wasdp2gh 39 4 fractions fraction NNS cord-022774-wasdp2gh 39 5 were be VBD cord-022774-wasdp2gh 39 6 fixed fix VBN cord-022774-wasdp2gh 39 7 after after IN cord-022774-wasdp2gh 39 8 temperature temperature NN cord-022774-wasdp2gh 39 9 incubation incubation NN cord-022774-wasdp2gh 39 10 in in IN cord-022774-wasdp2gh 39 11 2.0 2.0 CD cord-022774-wasdp2gh 39 12 ° ° NNS cord-022774-wasdp2gh 39 13 /0 /0 . cord-022774-wasdp2gh 40 1 glutaraldehyde glutaraldehyde VBN cord-022774-wasdp2gh 40 2 in in IN cord-022774-wasdp2gh 40 3 0.1 0.1 CD cord-022774-wasdp2gh 40 4 M m NN cord-022774-wasdp2gh 40 5 sodium sodium NN cord-022774-wasdp2gh 40 6 phosphate phosphate NN cord-022774-wasdp2gh 40 7 , , , cord-022774-wasdp2gh 40 8 pH pH NNP cord-022774-wasdp2gh 40 9 7.2 7.2 CD cord-022774-wasdp2gh 40 10 followed follow VBN cord-022774-wasdp2gh 40 11 by by IN cord-022774-wasdp2gh 40 12 post post NN cord-022774-wasdp2gh 40 13 fixation fixation NN cord-022774-wasdp2gh 40 14 in in IN cord-022774-wasdp2gh 40 15 osmium osmium NN cord-022774-wasdp2gh 40 16 tetroxide tetroxide NN cord-022774-wasdp2gh 40 17 in in IN cord-022774-wasdp2gh 40 18 the the DT cord-022774-wasdp2gh 40 19 same same JJ cord-022774-wasdp2gh 40 20 buffer buffer NN cord-022774-wasdp2gh 40 21 . . . cord-022774-wasdp2gh 41 1 Dehydration dehydration NN cord-022774-wasdp2gh 41 2 was be VBD cord-022774-wasdp2gh 41 3 through through IN cord-022774-wasdp2gh 41 4 an an DT cord-022774-wasdp2gh 41 5 acetone acetone NN cord-022774-wasdp2gh 41 6 series series NN cord-022774-wasdp2gh 41 7 with with IN cord-022774-wasdp2gh 41 8 embedment embedment NN cord-022774-wasdp2gh 41 9 in in IN cord-022774-wasdp2gh 41 10 Epon Epon NNP cord-022774-wasdp2gh 41 11 [ [ -LRB- cord-022774-wasdp2gh 41 12 11 11 CD cord-022774-wasdp2gh 41 13 ] ] -RRB- cord-022774-wasdp2gh 41 14 . . . cord-022774-wasdp2gh 42 1 Thin thin JJ cord-022774-wasdp2gh 42 2 sections section NNS cord-022774-wasdp2gh 42 3 were be VBD cord-022774-wasdp2gh 42 4 observed observe VBN cord-022774-wasdp2gh 42 5 and and CC cord-022774-wasdp2gh 42 6 photographed photograph VBN cord-022774-wasdp2gh 42 7 using use VBG cord-022774-wasdp2gh 42 8 a a DT cord-022774-wasdp2gh 42 9 Philips Philips NNP cord-022774-wasdp2gh 42 10 EM EM NNP cord-022774-wasdp2gh 42 11 200 200 CD cord-022774-wasdp2gh 42 12 electron electron NN cord-022774-wasdp2gh 42 13 microscope microscope NN cord-022774-wasdp2gh 42 14 . . . cord-022774-wasdp2gh 43 1 Endoplasmic Endoplasmic NNP cord-022774-wasdp2gh 43 2 reticulum reticulum NN cord-022774-wasdp2gh 43 3 ! ! . cord-022774-wasdp2gh 44 1 ractions raction NNS cord-022774-wasdp2gh 44 2 were be VBD cord-022774-wasdp2gh 44 3 isolated isolate VBN cord-022774-wasdp2gh 44 4 as as IN cord-022774-wasdp2gh 44 5 described describe VBN cord-022774-wasdp2gh 44 6 [ [ -LRB- cord-022774-wasdp2gh 44 7 13 13 CD cord-022774-wasdp2gh 44 8 ] ] -RRB- cord-022774-wasdp2gh 44 9 . . . cord-022774-wasdp2gh 45 1 Livers liver NNS cord-022774-wasdp2gh 45 2 were be VBD cord-022774-wasdp2gh 45 3 homogenized homogenize VBN cord-022774-wasdp2gh 45 4 in in IN cord-022774-wasdp2gh 45 5 2 2 CD cord-022774-wasdp2gh 45 6 vol vol NN cord-022774-wasdp2gh 45 7 of of IN cord-022774-wasdp2gh 45 8 a a DT cord-022774-wasdp2gh 45 9 medium medium NN cord-022774-wasdp2gh 45 10 containing contain VBG cord-022774-wasdp2gh 45 11 37.5 37.5 CD cord-022774-wasdp2gh 45 12 mM mm CD cord-022774-wasdp2gh 45 13 Tris Tris NNP cord-022774-wasdp2gh 45 14 maleate maleate NN cord-022774-wasdp2gh 45 15 , , , cord-022774-wasdp2gh 45 16 pH pH NNP cord-022774-wasdp2gh 45 17 6.5 6.5 CD cord-022774-wasdp2gh 46 1 , , , cord-022774-wasdp2gh 46 2 0.5 0.5 CD cord-022774-wasdp2gh 46 3 M M NNP cord-022774-wasdp2gh 46 4 sucrose sucrose NN cord-022774-wasdp2gh 46 5 , , , cord-022774-wasdp2gh 46 6 5 5 CD cord-022774-wasdp2gh 46 7 mM mm CD cord-022774-wasdp2gh 46 8 MgCI MgCI NNP cord-022774-wasdp2gh 46 9 , , , cord-022774-wasdp2gh 46 10 and and CC cord-022774-wasdp2gh 46 11 1 1 CD cord-022774-wasdp2gh 46 12 % % NN cord-022774-wasdp2gh 46 13 dextran dextran NN cord-022774-wasdp2gh 46 14 for for IN cord-022774-wasdp2gh 46 15 45 45 CD cord-022774-wasdp2gh 46 16 sec sec NNS cord-022774-wasdp2gh 46 17 with with IN cord-022774-wasdp2gh 46 18 a a DT cord-022774-wasdp2gh 46 19 Polytron Polytron NNP cord-022774-wasdp2gh 46 20 20 20 CD cord-022774-wasdp2gh 46 21 ST ST NNP cord-022774-wasdp2gh 46 22 operated operate VBD cord-022774-wasdp2gh 46 23 at at IN cord-022774-wasdp2gh 46 24 6,000 6,000 CD cord-022774-wasdp2gh 46 25 rpm rpm NN cord-022774-wasdp2gh 46 26 . . . cord-022774-wasdp2gh 47 1 The the DT cord-022774-wasdp2gh 47 2 homogenates homogenate NNS cord-022774-wasdp2gh 47 3 were be VBD cord-022774-wasdp2gh 47 4 centrifuged centrifuge VBN cord-022774-wasdp2gh 47 5 for for IN cord-022774-wasdp2gh 47 6 15 15 CD cord-022774-wasdp2gh 47 7 rain rain NN cord-022774-wasdp2gh 47 8 at at IN cord-022774-wasdp2gh 47 9 6,000 6,000 CD cord-022774-wasdp2gh 47 10 g g NN cord-022774-wasdp2gh 47 11 to to TO cord-022774-wasdp2gh 47 12 remove remove VB cord-022774-wasdp2gh 47 13 nuclei nucleus NNS cord-022774-wasdp2gh 47 14 , , , cord-022774-wasdp2gh 47 15 plasma plasma NN cord-022774-wasdp2gh 47 16 membrane membrane NN cord-022774-wasdp2gh 47 17 fragments fragment NNS cord-022774-wasdp2gh 47 18 and and CC cord-022774-wasdp2gh 47 19 Golgi Golgi NNP cord-022774-wasdp2gh 47 20 apparatus apparatus NN cord-022774-wasdp2gh 47 21 , , , cord-022774-wasdp2gh 47 22 and and CC cord-022774-wasdp2gh 47 23 the the DT cord-022774-wasdp2gh 47 24 supernatant supernatant JJ cord-022774-wasdp2gh 47 25 diluted dilute VBN cord-022774-wasdp2gh 47 26 1 1 CD cord-022774-wasdp2gh 47 27 : : SYM cord-022774-wasdp2gh 47 28 5 5 CD cord-022774-wasdp2gh 47 29 with with IN cord-022774-wasdp2gh 47 30 homogenization homogenization NN cord-022774-wasdp2gh 47 31 medium medium NN cord-022774-wasdp2gh 47 32 was be VBD cord-022774-wasdp2gh 47 33 centrifuged centrifuge VBN cord-022774-wasdp2gh 47 34 at at IN cord-022774-wasdp2gh 47 35 10,000 10,000 CD cord-022774-wasdp2gh 47 36 g g NN cord-022774-wasdp2gh 47 37 to to TO cord-022774-wasdp2gh 47 38 remove remove VB cord-022774-wasdp2gh 47 39 mitochondria mitochondrion NNS cord-022774-wasdp2gh 47 40 . . . cord-022774-wasdp2gh 48 1 The the DT cord-022774-wasdp2gh 48 2 supernatant supernatant NN cord-022774-wasdp2gh 48 3 containing contain VBG cord-022774-wasdp2gh 48 4 the the DT cord-022774-wasdp2gh 48 5 endop!asmic endop!asmic NN cord-022774-wasdp2gh 48 6 reticulum reticulum NN cord-022774-wasdp2gh 48 7 was be VBD cord-022774-wasdp2gh 48 8 layered layer VBN cord-022774-wasdp2gh 48 9 onto onto IN cord-022774-wasdp2gh 48 10 a a DT cord-022774-wasdp2gh 48 11 discontinuous discontinuous JJ cord-022774-wasdp2gh 48 12 sucrose sucrose NN cord-022774-wasdp2gh 48 13 gradient gradient NN cord-022774-wasdp2gh 48 14 consisting consist VBG cord-022774-wasdp2gh 48 15 of of IN cord-022774-wasdp2gh 48 16 2.0 2.0 CD cord-022774-wasdp2gh 48 17 , , , cord-022774-wasdp2gh 48 18 ! ! . cord-022774-wasdp2gh 48 19 .5 .5 NFP cord-022774-wasdp2gh 48 20 and and CC cord-022774-wasdp2gh 48 21 1.3 1.3 CD cord-022774-wasdp2gh 48 22 M M NNP cord-022774-wasdp2gh 48 23 sucrose sucrose NN cord-022774-wasdp2gh 48 24 layers layer NNS cord-022774-wasdp2gh 48 25 . . . cord-022774-wasdp2gh 49 1 After after IN cord-022774-wasdp2gh 49 2 centrifugation centrifugation NN cord-022774-wasdp2gh 49 3 at at IN cord-022774-wasdp2gh 49 4 85,000 85,000 CD cord-022774-wasdp2gh 49 5 g g NN cord-022774-wasdp2gh 49 6 for for IN cord-022774-wasdp2gh 49 7 90 90 CD cord-022774-wasdp2gh 49 8 min min NN cord-022774-wasdp2gh 49 9 , , , cord-022774-wasdp2gh 49 10 the the DT cord-022774-wasdp2gh 49 11 membranes membrane NNS cord-022774-wasdp2gh 49 12 from from IN cord-022774-wasdp2gh 49 13 the the DT cord-022774-wasdp2gh 49 14 1.3 1.3 CD cord-022774-wasdp2gh 49 15 M M NNP cord-022774-wasdp2gh 49 16 sucrose sucrose NN cord-022774-wasdp2gh 49 17 / / SYM cord-022774-wasdp2gh 49 18 sample sample NN cord-022774-wasdp2gh 49 19 interface interface NN cord-022774-wasdp2gh 49 20 were be VBD cord-022774-wasdp2gh 49 21 withdrawn withdraw VBN cord-022774-wasdp2gh 49 22 using use VBG cord-022774-wasdp2gh 49 23 a a DT cord-022774-wasdp2gh 49 24 Pasteur Pasteur NNP cord-022774-wasdp2gh 49 25 pipette pipette NN cord-022774-wasdp2gh 49 26 and and CC cord-022774-wasdp2gh 49 27 pelleted pellete VBN cord-022774-wasdp2gh 49 28 by by IN cord-022774-wasdp2gh 49 29 centrifugation centrifugation NN cord-022774-wasdp2gh 49 30 for for IN cord-022774-wasdp2gh 49 31 20 20 CD cord-022774-wasdp2gh 49 32 rain rain NN cord-022774-wasdp2gh 49 33 at at IN cord-022774-wasdp2gh 49 34 70,000 70,000 CD cord-022774-wasdp2gh 49 35 g g NN cord-022774-wasdp2gh 49 36 as as IN cord-022774-wasdp2gh 49 37 the the DT cord-022774-wasdp2gh 49 38 starting start VBG cord-022774-wasdp2gh 49 39 fraction fraction NN cord-022774-wasdp2gh 49 40 for for IN cord-022774-wasdp2gh 49 41 temperature temperature NN cord-022774-wasdp2gh 49 42 incubation incubation NN cord-022774-wasdp2gh 49 43 in in IN cord-022774-wasdp2gh 49 44 a'reconstituted a'reconstituted JJ cord-022774-wasdp2gh 49 45 system system NN cord-022774-wasdp2gh 49 46 capable capable JJ cord-022774-wasdp2gh 49 47 of of IN cord-022774-wasdp2gh 49 48 in in FW cord-022774-wasdp2gh 49 49 vitro vitro FW cord-022774-wasdp2gh 49 50 formation formation NN cord-022774-wasdp2gh 49 51 of of IN cord-022774-wasdp2gh 49 52 transition transition NN cord-022774-wasdp2gh 49 53 vesicles vesicle NNS cord-022774-wasdp2gh 49 54 ( ( -LRB- cord-022774-wasdp2gh 49 55 see see VB cord-022774-wasdp2gh 49 56 below below RB cord-022774-wasdp2gh 49 57 ) ) -RRB- cord-022774-wasdp2gh 49 58 . . . cord-022774-wasdp2gh 50 1 Part part NN cord-022774-wasdp2gh 50 2 - - HYPH cord-022774-wasdp2gh 50 3 rough rough JJ cord-022774-wasdp2gh 50 4 , , , cord-022774-wasdp2gh 50 5 part part NN cord-022774-wasdp2gh 50 6 - - HYPH cord-022774-wasdp2gh 50 7 smooth smooth JJ cord-022774-wasdp2gh 50 8 transition transition NN cord-022774-wasdp2gh 50 9 elements element NNS cord-022774-wasdp2gh 50 10 of of IN cord-022774-wasdp2gh 50 11 endoplasmic endoplasmic NN cord-022774-wasdp2gh 50 12 reticulum reticulum NN cord-022774-wasdp2gh 50 13 were be VBD cord-022774-wasdp2gh 50 14 isolated isolate VBN cord-022774-wasdp2gh 50 15 as as IN cord-022774-wasdp2gh 50 16 described describe VBN cord-022774-wasdp2gh 50 17 above above RB cord-022774-wasdp2gh 50 18 and and CC cord-022774-wasdp2gh 50 19 incubated incubate VBN cord-022774-wasdp2gh 50 20 in in IN cord-022774-wasdp2gh 50 21 the the DT cord-022774-wasdp2gh 50 22 presence presence NN cord-022774-wasdp2gh 50 23 of of IN cord-022774-wasdp2gh 50 24 nucleoside nucleoside NN cord-022774-wasdp2gh 50 25 triphosphate triphosphate NN cord-022774-wasdp2gh 50 26 and and CC cord-022774-wasdp2gh 50 27 cytosol cytosol NN cord-022774-wasdp2gh 50 28 at at IN cord-022774-wasdp2gh 50 29 either either DT cord-022774-wasdp2gh 50 30 4 4 CD cord-022774-wasdp2gh 50 31 ° ° NN cord-022774-wasdp2gh 50 32 C c NN cord-022774-wasdp2gh 50 33 or or CC cord-022774-wasdp2gh 50 34 37 37 CD cord-022774-wasdp2gh 50 35 ° ° NN cord-022774-wasdp2gh 50 36 C c NN cord-022774-wasdp2gh 50 37 . . . cord-022774-wasdp2gh 51 1 In in IN cord-022774-wasdp2gh 51 2 addition addition NN cord-022774-wasdp2gh 51 3 , , , cord-022774-wasdp2gh 51 4 the the DT cord-022774-wasdp2gh 51 5 complete complete JJ cord-022774-wasdp2gh 51 6 incubation incubation NN cord-022774-wasdp2gh 51 7 systems system NNS cord-022774-wasdp2gh 51 8 contained contain VBD cord-022774-wasdp2gh 51 9 an an DT cord-022774-wasdp2gh 51 10 ATP ATP NNP cord-022774-wasdp2gh 51 11 regenerating regenerate VBG cord-022774-wasdp2gh 51 12 system system NN cord-022774-wasdp2gh 51 13 of of IN cord-022774-wasdp2gh 51 14 30 30 CD cord-022774-wasdp2gh 51 15 mM mm CD cord-022774-wasdp2gh 51 16 HEPES HEPES NNP cord-022774-wasdp2gh 51 17 - - HYPH cord-022774-wasdp2gh 51 18 KOH KOH NNP cord-022774-wasdp2gh 51 19 , , , cord-022774-wasdp2gh 51 20 30 30 CD cord-022774-wasdp2gh 51 21 mM mm CD cord-022774-wasdp2gh 51 22 KCI KCI NNP cord-022774-wasdp2gh 51 23 , , , cord-022774-wasdp2gh 51 24 2.5 2.5 CD cord-022774-wasdp2gh 51 25 mM mm CD cord-022774-wasdp2gh 51 26 Mg Mg NNP cord-022774-wasdp2gh 51 27 acetate acetate NN cord-022774-wasdp2gh 51 28 , , , cord-022774-wasdp2gh 51 29 80 80 CD cord-022774-wasdp2gh 51 30 pM pm NN cord-022774-wasdp2gh 52 1 ATP ATP NNP cord-022774-wasdp2gh 52 2 , , , cord-022774-wasdp2gh 52 3 300/~M 300/~m CD cord-022774-wasdp2gh 52 4 UTP UTP NNP cord-022774-wasdp2gh 52 5 , , , cord-022774-wasdp2gh 52 6 2 2 CD cord-022774-wasdp2gh 52 7 mM mm NN cord-022774-wasdp2gh 52 8 creatine creatine NN cord-022774-wasdp2gh 52 9 phosphate phosphate NN cord-022774-wasdp2gh 52 10 and and CC cord-022774-wasdp2gh 52 11 10 10 CD cord-022774-wasdp2gh 52 12 IU IU NNP cord-022774-wasdp2gh 52 13 / / , cord-022774-wasdp2gh 52 14 ml ml NN cord-022774-wasdp2gh 52 15 creatine creatine NN cord-022774-wasdp2gh 52 16 phosphokinase phosphokinase NN cord-022774-wasdp2gh 52 17 ( ( -LRB- cord-022774-wasdp2gh 52 18 final final JJ cord-022774-wasdp2gh 52 19 pH pH NNP cord-022774-wasdp2gh 52 20 7.0 7.0 CD cord-022774-wasdp2gh 52 21 ) ) -RRB- cord-022774-wasdp2gh 52 22 [ [ -LRB- cord-022774-wasdp2gh 52 23 15 15 CD cord-022774-wasdp2gh 52 24 , , , cord-022774-wasdp2gh 52 25 161 161 CD cord-022774-wasdp2gh 52 26 . . . cord-022774-wasdp2gh 52 27 _SP cord-022774-wasdp2gh 53 1 Part part NN cord-022774-wasdp2gh 53 2 - - HYPH cord-022774-wasdp2gh 53 3 rough rough JJ cord-022774-wasdp2gh 53 4 , , , cord-022774-wasdp2gh 53 5 part part NN cord-022774-wasdp2gh 53 6 - - HYPH cord-022774-wasdp2gh 53 7 smooth smooth JJ cord-022774-wasdp2gh 53 8 fractions fraction NNS cord-022774-wasdp2gh 53 9 of of IN cord-022774-wasdp2gh 53 10 the the DT cord-022774-wasdp2gh 53 11 transition transition NN cord-022774-wasdp2gh 53 12 endoplasmic endoplasmic NN cord-022774-wasdp2gh 53 13 reticulum reticulum NN cord-022774-wasdp2gh 53 14 fraction fraction NN cord-022774-wasdp2gh 53 15 from from IN cord-022774-wasdp2gh 53 16 rat rat NN cord-022774-wasdp2gh 53 17 liver liver NN cord-022774-wasdp2gh 53 18 when when WRB cord-022774-wasdp2gh 53 19 resuspended resuspend VBN cord-022774-wasdp2gh 53 20 in in IN cord-022774-wasdp2gh 53 21 ATP ATP NNP cord-022774-wasdp2gh 53 22 , , , cord-022774-wasdp2gh 53 23 ATP ATP NNP cord-022774-wasdp2gh 53 24 regenerating regenerate VBG cord-022774-wasdp2gh 53 25 system system NN cord-022774-wasdp2gh 53 26 and and CC cord-022774-wasdp2gh 53 27 c3~osol c3~osol NNP cord-022774-wasdp2gh 53 28 , , , cord-022774-wasdp2gh 53 29 were be VBD cord-022774-wasdp2gh 53 30 observed observe VBN cord-022774-wasdp2gh 53 31 to to TO cord-022774-wasdp2gh 53 32 contain contain VB cord-022774-wasdp2gh 53 33 = = SYM cord-022774-wasdp2gh 53 34 60 60 CD cord-022774-wasdp2gh 53 35 nm nm NNP cord-022774-wasdp2gh 53 36 bebs bebs NN cord-022774-wasdp2gh 53 37 and and CC cord-022774-wasdp2gh 53 38 vesicles vesicle NNS cord-022774-wasdp2gh 53 39 reminiscent reminiscent JJ cord-022774-wasdp2gh 53 40 of of IN cord-022774-wasdp2gh 53 41 transition transition NN cord-022774-wasdp2gh 53 42 vesicles vesicle NNS cord-022774-wasdp2gh 53 43 produced produce VBN cord-022774-wasdp2gh 53 44 in in IN cord-022774-wasdp2gh 53 45 situ situ FW cord-022774-wasdp2gh 53 46 ( ( -LRB- cord-022774-wasdp2gh 53 47 Fig fig NN cord-022774-wasdp2gh 53 48 . . . cord-022774-wasdp2gh 53 49 1 1 CD cord-022774-wasdp2gh 53 50 ; ; : cord-022774-wasdp2gh 53 51 see see VB cord-022774-wasdp2gh 53 52 Nowack Nowack NNP cord-022774-wasdp2gh 53 53 et et FW cord-022774-wasdp2gh 53 54 al al NNP cord-022774-wasdp2gh 53 55 . . . cord-022774-wasdp2gh 54 1 [ [ -LRB- cord-022774-wasdp2gh 54 2 IS be VBZ cord-022774-wasdp2gh 54 3 ] ] -RRB- cord-022774-wasdp2gh 54 4 for for IN cord-022774-wasdp2gh 54 5 a a DT cord-022774-wasdp2gh 54 6 comparison comparison NN cord-022774-wasdp2gh 54 7 ) ) -RRB- cord-022774-wasdp2gh 54 8 . . . cord-022774-wasdp2gh 55 1 These these DT cord-022774-wasdp2gh 55 2 vesicles vesicle NNS cord-022774-wasdp2gh 55 3 appeared appear VBD cord-022774-wasdp2gh 55 4 to to TO cord-022774-wasdp2gh 55 5 vary vary VB cord-022774-wasdp2gh 55 6 in in IN cord-022774-wasdp2gh 55 7 number number NN cord-022774-wasdp2gh 55 8 as as IN cord-022774-wasdp2gh 55 9 a a DT cord-022774-wasdp2gh 55 10 result result NN cord-022774-wasdp2gh 55 11 of of IN cord-022774-wasdp2gh 55 12 incubation incubation NN cord-022774-wasdp2gh 55 13 at at IN cord-022774-wasdp2gh 55 14 different different JJ cord-022774-wasdp2gh 55 15 temperatures temperature NNS cord-022774-wasdp2gh 55 16 . . . cord-022774-wasdp2gh 56 1 To to TO cord-022774-wasdp2gh 56 2 quantitate quantitate VB cord-022774-wasdp2gh 56 3 the the DT cord-022774-wasdp2gh 56 4 numbers number NNS cord-022774-wasdp2gh 56 5 of of IN cord-022774-wasdp2gh 56 6 vesicles vesicle NNS cord-022774-wasdp2gh 56 7 present present JJ cord-022774-wasdp2gh 56 8 , , , cord-022774-wasdp2gh 56 9 the the DT cord-022774-wasdp2gh 56 10 preparations preparation NNS cord-022774-wasdp2gh 56 11 were be VBD cord-022774-wasdp2gh 56 12 pelleted pellete VBN cord-022774-wasdp2gh 56 13 onto onto IN cord-022774-wasdp2gh 56 14 nitrocellulose nitrocellulose JJ cord-022774-wasdp2gh 56 15 filters filter NNS cord-022774-wasdp2gh 56 16 , , , cord-022774-wasdp2gh 56 17 prepared prepare VBN cord-022774-wasdp2gh 56 18 for for IN cord-022774-wasdp2gh 56 19 electron electron NN cord-022774-wasdp2gh 56 20 microscopy microscopy NN cord-022774-wasdp2gh 56 21 , , , cord-022774-wasdp2gh 56 22 and and CC cord-022774-wasdp2gh 56 23 the the DT cord-022774-wasdp2gh 56 24 pellets pellet NNS cord-022774-wasdp2gh 56 25 were be VBD cord-022774-wasdp2gh 56 26 photographed photograph VBN cord-022774-wasdp2gh 56 27 through through IN cord-022774-wasdp2gh 56 28 their -PRON- PRP$ cord-022774-wasdp2gh 56 29 entire entire JJ cord-022774-wasdp2gh 56 30 cross cross JJ cord-022774-wasdp2gh 56 31 sectional sectional JJ cord-022774-wasdp2gh 56 32 area area NN cord-022774-wasdp2gh 56 33 . . . cord-022774-wasdp2gh 57 1 All all DT cord-022774-wasdp2gh 57 2 vesicles vesicle NNS cord-022774-wasdp2gh 57 3 were be VBD cord-022774-wasdp2gh 57 4 counted count VBN cord-022774-wasdp2gh 57 5 in in IN cord-022774-wasdp2gh 57 6 3 3 CD cord-022774-wasdp2gh 57 7 representative representative NN cord-022774-wasdp2gh 57 8 40/~m 40/~m CD cord-022774-wasdp2gh 57 9 2 2 CD cord-022774-wasdp2gh 57 10 areas area NNS cord-022774-wasdp2gh 57 11 . . . cord-022774-wasdp2gh 58 1 Only only RB cord-022774-wasdp2gh 58 2 circular circular JJ cord-022774-wasdp2gh 58 3 profiles profile NNS cord-022774-wasdp2gh 58 4 with with IN cord-022774-wasdp2gh 58 5 solid solid JJ cord-022774-wasdp2gh 58 6 interiors interior NNS cord-022774-wasdp2gh 58 7 representing represent VBG cord-022774-wasdp2gh 58 8 filled fill VBN cord-022774-wasdp2gh 58 9 vesicles vesicle NNS cord-022774-wasdp2gh 58 10 rather rather RB cord-022774-wasdp2gh 58 11 than than IN cord-022774-wasdp2gh 58 12 cross cross VB cord-022774-wasdp2gh 58 13 sections section NNS cord-022774-wasdp2gh 58 14 of of IN cord-022774-wasdp2gh 58 15 hollow hollow JJ cord-022774-wasdp2gh 58 16 tubules tubule NNS cord-022774-wasdp2gh 58 17 , , , cord-022774-wasdp2gh 58 18 and and CC cord-022774-wasdp2gh 58 19 in in IN cord-022774-wasdp2gh 58 20 the the DT cord-022774-wasdp2gh 58 21 approximate approximate JJ cord-022774-wasdp2gh 58 22 size size NN cord-022774-wasdp2gh 58 23 range range NN cord-022774-wasdp2gh 58 24 to to TO cord-022774-wasdp2gh 58 25 be be VB cord-022774-wasdp2gh 58 26 transition transition NN cord-022774-wasdp2gh 58 27 vesicles vesicle NNS cord-022774-wasdp2gh 58 28 were be VBD cord-022774-wasdp2gh 58 29 scored score VBN cord-022774-wasdp2gh 58 30 as as IN cord-022774-wasdp2gh 58 31 transition transition NN cord-022774-wasdp2gh 58 32 vesicles vesicle NNS cord-022774-wasdp2gh 58 33 . . . cord-022774-wasdp2gh 59 1 Many many JJ cord-022774-wasdp2gh 59 2 were be VBD cord-022774-wasdp2gh 59 3 still still RB cord-022774-wasdp2gh 59 4 connected connect VBN cord-022774-wasdp2gh 59 5 to to IN cord-022774-wasdp2gh 59 6 the the DT cord-022774-wasdp2gh 59 7 transitional transitional JJ cord-022774-wasdp2gh 59 8 endoplasmic endoplasmic NN cord-022774-wasdp2gh 59 9 reticulum reticulum NN cord-022774-wasdp2gh 59 10 membranes membrane NNS cord-022774-wasdp2gh 59 11 by by IN cord-022774-wasdp2gh 59 12 a a DT cord-022774-wasdp2gh 59 13 narrow narrow JJ cord-022774-wasdp2gh 59 14 stalk stalk NN cord-022774-wasdp2gh 59 15 ( ( -LRB- cord-022774-wasdp2gh 59 16 Fig Fig NNP cord-022774-wasdp2gh 59 17 . . NNP cord-022774-wasdp2gh 59 18 1 1 CD cord-022774-wasdp2gh 59 19 , , , cord-022774-wasdp2gh 59 20 inset inset NN cord-022774-wasdp2gh 59 21 ) ) -RRB- cord-022774-wasdp2gh 59 22 . . . cord-022774-wasdp2gh 60 1 The the DT cord-022774-wasdp2gh 60 2 numbers number NNS cord-022774-wasdp2gh 60 3 of of IN cord-022774-wasdp2gh 60 4 putative putative JJ cord-022774-wasdp2gh 60 5 transition transition NN cord-022774-wasdp2gh 60 6 vesicles vesicle NNS cord-022774-wasdp2gh 60 7 present present JJ cord-022774-wasdp2gh 60 8 in in IN cord-022774-wasdp2gh 60 9 the the DT cord-022774-wasdp2gh 60 10 resuspended resuspended JJ cord-022774-wasdp2gh 60 11 membranes membrane NNS cord-022774-wasdp2gh 60 12 was be VBD cord-022774-wasdp2gh 60 13 approximately approximately RB cord-022774-wasdp2gh 60 14 1.5 1.5 CD cord-022774-wasdp2gh 60 15 membrane membrane NN cord-022774-wasdp2gh 60 16 profiles profile NNS cord-022774-wasdp2gh 60 17 per per IN cord-022774-wasdp2gh 60 18 100 100 CD cord-022774-wasdp2gh 60 19 membrane membrane NN cord-022774-wasdp2gh 60 20 profiles profile NNS cord-022774-wasdp2gh 60 21 at at IN cord-022774-wasdp2gh 60 22 4 4 CD cord-022774-wasdp2gh 60 23 ° ° NN cord-022774-wasdp2gh 60 24 C c NN cord-022774-wasdp2gh 60 25 ( ( -LRB- cord-022774-wasdp2gh 60 26 Fig Fig NNP cord-022774-wasdp2gh 60 27 . . NNP cord-022774-wasdp2gh 60 28 2 2 CD cord-022774-wasdp2gh 60 29 ) ) -RRB- cord-022774-wasdp2gh 60 30 . . . cord-022774-wasdp2gh 61 1 With with IN cord-022774-wasdp2gh 61 2 increasing increase VBG cord-022774-wasdp2gh 61 3 temperature temperature NN cord-022774-wasdp2gh 61 4 , , , cord-022774-wasdp2gh 61 5 the the DT cord-022774-wasdp2gh 61 6 number number NN cord-022774-wasdp2gh 61 7 of of IN cord-022774-wasdp2gh 61 8 additional additional JJ cord-022774-wasdp2gh 61 9 vesicles vesicle NNS cord-022774-wasdp2gh 61 10 followed follow VBD cord-022774-wasdp2gh 61 11 a a DT cord-022774-wasdp2gh 61 12 hyperbolic hyperbolic JJ cord-022774-wasdp2gh 61 13 function function NN cord-022774-wasdp2gh 61 14 of of IN cord-022774-wasdp2gh 61 15 increase increase NN cord-022774-wasdp2gh 61 16 to to IN cord-022774-wasdp2gh 61 17 approximatively approximatively RB cord-022774-wasdp2gh 61 18 double double VB cord-022774-wasdp2gh 61 19 at at IN cord-022774-wasdp2gh 61 20 37 37 CD cord-022774-wasdp2gh 61 21 ° ° NN cord-022774-wasdp2gh 61 22 C c NN cord-022774-wasdp2gh 61 23 with with IN cord-022774-wasdp2gh 61 24 Qi0 qi0 NN cord-022774-wasdp2gh 61 25 of of IN cord-022774-wasdp2gh 61 26 increase increase NN cord-022774-wasdp2gh 61 27 of of IN cord-022774-wasdp2gh 61 28 about about RB cord-022774-wasdp2gh 61 29 2 2 CD cord-022774-wasdp2gh 61 30 over over IN cord-022774-wasdp2gh 61 31 the the DT cord-022774-wasdp2gh 61 32 temperature temperature NN cord-022774-wasdp2gh 61 33 range range NN cord-022774-wasdp2gh 61 34 12 12 CD cord-022774-wasdp2gh 61 35 ° ° NN cord-022774-wasdp2gh 61 36 C c NN cord-022774-wasdp2gh 61 37 to to IN cord-022774-wasdp2gh 61 38 37 37 CD cord-022774-wasdp2gh 61 39 ° ° NN cord-022774-wasdp2gh 61 40 C c NN cord-022774-wasdp2gh 61 41 . . . cord-022774-wasdp2gh 62 1 By by IN cord-022774-wasdp2gh 62 2 extrapolation extrapolation NN cord-022774-wasdp2gh 62 3 , , , cord-022774-wasdp2gh 62 4 the the DT cord-022774-wasdp2gh 62 5 temperature temperature NN cord-022774-wasdp2gh 62 6 dependent dependent JJ cord-022774-wasdp2gh 62 7 formation formation NN cord-022774-wasdp2gh 62 8 of of IN cord-022774-wasdp2gh 62 9 60 60 CD cord-022774-wasdp2gh 62 10 nm nm NN cord-022774-wasdp2gh 62 11 vesicles vesicle NNS cord-022774-wasdp2gh 62 12 below below IN cord-022774-wasdp2gh 62 13 16 16 CD cord-022774-wasdp2gh 62 14 ° ° NN cord-022774-wasdp2gh 62 15 C C NNP cord-022774-wasdp2gh 62 16 was be VBD cord-022774-wasdp2gh 62 17 insignificant insignificant JJ cord-022774-wasdp2gh 62 18 . . . cord-022774-wasdp2gh 63 1 The the DT cord-022774-wasdp2gh 63 2 effect effect NN cord-022774-wasdp2gh 63 3 of of IN cord-022774-wasdp2gh 63 4 temperature temperature NN cord-022774-wasdp2gh 63 5 on on IN cord-022774-wasdp2gh 63 6 transition transition NN cord-022774-wasdp2gh 63 7 vesicle vesicle NN cord-022774-wasdp2gh 63 8 production production NN cord-022774-wasdp2gh 63 9 in in IN cord-022774-wasdp2gh 63 10 situ situ NNS cord-022774-wasdp2gh 63 11 was be VBD cord-022774-wasdp2gh 63 12 examined examine VBN cord-022774-wasdp2gh 63 13 with with IN cord-022774-wasdp2gh 63 14 liver liver NN cord-022774-wasdp2gh 63 15 slices slice NNS cord-022774-wasdp2gh 63 16 as as RB cord-022774-wasdp2gh 63 17 well well RB cord-022774-wasdp2gh 63 18 . . . cord-022774-wasdp2gh 64 1 The the DT cord-022774-wasdp2gh 64 2 morphology morphology NN cord-022774-wasdp2gh 64 3 of of IN cord-022774-wasdp2gh 64 4 the the DT cord-022774-wasdp2gh 64 5 Golgi Golgi NNP cord-022774-wasdp2gh 64 6 apparatus apparatus NN cord-022774-wasdp2gh 64 7 of of IN cord-022774-wasdp2gh 64 8 the the DT cord-022774-wasdp2gh 64 9 slices slice NNS cord-022774-wasdp2gh 64 10 at at IN cord-022774-wasdp2gh 64 11 8 8 CD cord-022774-wasdp2gh 64 12 ° ° NN cord-022774-wasdp2gh 64 13 C c NN cord-022774-wasdp2gh 64 14 ( ( -LRB- cord-022774-wasdp2gh 64 15 Figs fig NNS cord-022774-wasdp2gh 64 16 . . . cord-022774-wasdp2gh 65 1 3A 3A NNP cord-022774-wasdp2gh 65 2 and and CC cord-022774-wasdp2gh 65 3 4A 4a LS cord-022774-wasdp2gh 65 4 ) ) -RRB- cord-022774-wasdp2gh 65 5 was be VBD cord-022774-wasdp2gh 65 6 representative representative NN cord-022774-wasdp2gh 65 7 of of IN cord-022774-wasdp2gh 65 8 that that DT cord-022774-wasdp2gh 66 1 observed observe VBD cord-022774-wasdp2gh 66 2 both both CC cord-022774-wasdp2gh 66 3 at at IN cord-022774-wasdp2gh 66 4 4 4 CD cord-022774-wasdp2gh 66 5 ° ° NN cord-022774-wasdp2gh 66 6 C c NN cord-022774-wasdp2gh 66 7 and and CC cord-022774-wasdp2gh 66 8 at at IN cord-022774-wasdp2gh 66 9 12 12 CD cord-022774-wasdp2gh 66 10 ° ° NN cord-022774-wasdp2gh 66 11 C c NN cord-022774-wasdp2gh 66 12 . . . cord-022774-wasdp2gh 67 1 Golgi golgi NN cord-022774-wasdp2gh 67 2 apparatus apparatus NN cord-022774-wasdp2gh 67 3 with with IN cord-022774-wasdp2gh 67 4 characteristic characteristic JJ cord-022774-wasdp2gh 67 5 stacked stack VBN cord-022774-wasdp2gh 67 6 cisternae cisternae NNP cord-022774-wasdp2gh 67 7 were be VBD cord-022774-wasdp2gh 67 8 present present JJ cord-022774-wasdp2gh 67 9 at at IN cord-022774-wasdp2gh 67 10 all all DT cord-022774-wasdp2gh 67 11 temperatures temperature NNS cord-022774-wasdp2gh 67 12 so so IN cord-022774-wasdp2gh 67 13 that that IN cord-022774-wasdp2gh 67 14 Golgi Golgi NNP cord-022774-wasdp2gh 67 15 apparatus apparatus NN cord-022774-wasdp2gh 67 16 zones zone NNS cord-022774-wasdp2gh 67 17 could could MD cord-022774-wasdp2gh 67 18 be be VB cord-022774-wasdp2gh 67 19 readily readily RB cord-022774-wasdp2gh 67 20 located locate VBN cord-022774-wasdp2gh 67 21 . . . cord-022774-wasdp2gh 68 1 Over over IN cord-022774-wasdp2gh 68 2 the the DT cord-022774-wasdp2gh 68 3 temperature temperature NN cord-022774-wasdp2gh 68 4 range range NN cord-022774-wasdp2gh 68 5 4 4 CD cord-022774-wasdp2gh 68 6 ° ° NN cord-022774-wasdp2gh 68 7 C c NN cord-022774-wasdp2gh 68 8 to to IN cord-022774-wasdp2gh 68 9 12 12 CD cord-022774-wasdp2gh 68 10 ° ° NN cord-022774-wasdp2gh 68 11 C c NN cord-022774-wasdp2gh 68 12 , , , cord-022774-wasdp2gh 68 13 transition transition NN cord-022774-wasdp2gh 68 14 vesicles vesicle NNS cord-022774-wasdp2gh 68 15 were be VBD cord-022774-wasdp2gh 68 16 sparse sparse JJ cord-022774-wasdp2gh 68 17 . . . cord-022774-wasdp2gh 69 1 However however RB cord-022774-wasdp2gh 69 2 , , , cord-022774-wasdp2gh 69 3 transition transition NN cord-022774-wasdp2gh 69 4 elements element NNS cord-022774-wasdp2gh 69 5 were be VBD cord-022774-wasdp2gh 69 6 well well RB cord-022774-wasdp2gh 69 7 developed developed JJ cord-022774-wasdp2gh 69 8 and and CC cord-022774-wasdp2gh 69 9 numerous numerous JJ cord-022774-wasdp2gh 69 10 tubular tubular JJ cord-022774-wasdp2gh 69 11 smooth smooth JJ cord-022774-wasdp2gh 69 12 elements element NNS cord-022774-wasdp2gh 69 13 of of IN cord-022774-wasdp2gh 69 14 the the DT cord-022774-wasdp2gh 69 15 endoplasmic endoplasmic NN cord-022774-wasdp2gh 69 16 reticulum reticulum NN cord-022774-wasdp2gh 69 17 were be VBD cord-022774-wasdp2gh 69 18 evident evident JJ cord-022774-wasdp2gh 69 19 within within IN cord-022774-wasdp2gh 69 20 the the DT cord-022774-wasdp2gh 69 21 Golgi Golgi NNP cord-022774-wasdp2gh 69 22 zone zone NN cord-022774-wasdp2gh 69 23 adjacent adjacent JJ cord-022774-wasdp2gh 69 24 to to IN cord-022774-wasdp2gh 69 25 the the DT cord-022774-wasdp2gh 69 26 c c NN cord-022774-wasdp2gh 69 27 / / SYM cord-022774-wasdp2gh 69 28 s s NNP cord-022774-wasdp2gh 69 29 Golgi Golgi NNP cord-022774-wasdp2gh 69 30 apparatus apparatus NN cord-022774-wasdp2gh 69 31 face face NN cord-022774-wasdp2gh 69 32 . . . cord-022774-wasdp2gh 70 1 These these DT cord-022774-wasdp2gh 70 2 tubular tubular JJ cord-022774-wasdp2gh 70 3 smooth smooth RB cord-022774-wasdp2gh 70 4 - - HYPH cord-022774-wasdp2gh 70 5 surfaced surface VBN cord-022774-wasdp2gh 70 6 membranes membrane NNS cord-022774-wasdp2gh 70 7 were be VBD cord-022774-wasdp2gh 70 8 more more RBR cord-022774-wasdp2gh 70 9 evident evident JJ cord-022774-wasdp2gh 70 10 after after IN cord-022774-wasdp2gh 70 11 60 60 CD cord-022774-wasdp2gh 70 12 rain rain NN cord-022774-wasdp2gh 70 13 of of IN cord-022774-wasdp2gh 70 14 incubation incubation NN cord-022774-wasdp2gh 70 15 ( ( -LRB- cord-022774-wasdp2gh 70 16 Fig Fig NNP cord-022774-wasdp2gh 70 17 . . . cord-022774-wasdp2gh 70 18 4A 4a CD cord-022774-wasdp2gh 70 19 ) ) -RRB- cord-022774-wasdp2gh 70 20 than than IN cord-022774-wasdp2gh 70 21 after after IN cord-022774-wasdp2gh 70 22 30 30 CD cord-022774-wasdp2gh 70 23 rain rain NN cord-022774-wasdp2gh 70 24 of of IN cord-022774-wasdp2gh 70 25 incubation incubation NN cord-022774-wasdp2gh 70 26 ( ( -LRB- cord-022774-wasdp2gh 70 27 Fig Fig NNP cord-022774-wasdp2gh 70 28 . . . cord-022774-wasdp2gh 70 29 3A 3A NNP cord-022774-wasdp2gh 70 30 ) ) -RRB- cord-022774-wasdp2gh 70 31 . . . cord-022774-wasdp2gh 71 1 With with IN cord-022774-wasdp2gh 71 2 slices slice NNS cord-022774-wasdp2gh 71 3 incubated incubate VBN cord-022774-wasdp2gh 71 4 at at IN cord-022774-wasdp2gh 71 5 16 16 CD cord-022774-wasdp2gh 71 6 ° ° NN cord-022774-wasdp2gh 71 7 C c NN cord-022774-wasdp2gh 71 8 ( ( -LRB- cord-022774-wasdp2gh 71 9 Fig Fig NNP cord-022774-wasdp2gh 71 10 . . . cord-022774-wasdp2gh 71 11 4B 4b LS cord-022774-wasdp2gh 71 12 ) ) -RRB- cord-022774-wasdp2gh 71 13 or or CC cord-022774-wasdp2gh 71 14 18 18 CD cord-022774-wasdp2gh 71 15 ° ° NN cord-022774-wasdp2gh 71 16 C c NN cord-022774-wasdp2gh 71 17 ( ( -LRB- cord-022774-wasdp2gh 71 18 Fig Fig NNP cord-022774-wasdp2gh 71 19 . . . cord-022774-wasdp2gh 71 20 _SP cord-022774-wasdp2gh 71 21 3B 3B NNP cord-022774-wasdp2gh 71 22 and and CC cord-022774-wasdp2gh 71 23 4C 4c CD cord-022774-wasdp2gh 71 24 ) ) -RRB- cord-022774-wasdp2gh 71 25 , , , cord-022774-wasdp2gh 71 26 the the DT cord-022774-wasdp2gh 71 27 most most RBS cord-022774-wasdp2gh 71 28 obvious obvious JJ cord-022774-wasdp2gh 71 29 morphological morphological JJ cord-022774-wasdp2gh 71 30 aReration areration NN cord-022774-wasdp2gh 71 31 from from IN cord-022774-wasdp2gh 71 32 the the DT cord-022774-wasdp2gh 71 33 appearance appearance NN cord-022774-wasdp2gh 71 34 at at IN cord-022774-wasdp2gh 71 35 12cC 12cc CD cord-022774-wasdp2gh 71 36 was be VBD cord-022774-wasdp2gh 71 37 an an DT cord-022774-wasdp2gh 71 38 i i NN cord-022774-wasdp2gh 71 39 ~ ~ CD cord-022774-wasdp2gh 71 40 crease crease NN cord-022774-wasdp2gh 71 41 in in IN cord-022774-wasdp2gh 71 42 the the DT cord-022774-wasdp2gh 71 43 number number NN cord-022774-wasdp2gh 71 44 of of IN cord-022774-wasdp2gh 71 45 transition transition NN cord-022774-wasdp2gh 71 46 vesicles vesicle NNS cord-022774-wasdp2gh 71 47 . . . cord-022774-wasdp2gh 72 1 Especially especially RB cord-022774-wasdp2gh 72 2 with with IN cord-022774-wasdp2gh 72 3 60 60 CD cord-022774-wasdp2gh 72 4 rain rain NN cord-022774-wasdp2gh 72 5 of of IN cord-022774-wasdp2gh 72 6 incubation incubation NN cord-022774-wasdp2gh 72 7 , , , cord-022774-wasdp2gh 72 8 the the DT cord-022774-wasdp2gh 72 9 vesicles vesicle NNS cord-022774-wasdp2gh 72 10 accumulated accumulate VBN cord-022774-wasdp2gh 72 11 in in IN cord-022774-wasdp2gh 72 12 clusters cluster NNS cord-022774-wasdp2gh 72 13 , , , cord-022774-wasdp2gh 72 14 usually usually RB cord-022774-wasdp2gh 72 15 near near IN cord-022774-wasdp2gh 72 16 the the DT cord-022774-wasdp2gh 72 17 cis cis NNP cord-022774-wasdp2gh 72 18 Golgi Golgi NNP cord-022774-wasdp2gh 72 19 apparatus apparatus NN cord-022774-wasdp2gh 72 20 face face NN cord-022774-wasdp2gh 72 21 between between IN cord-022774-wasdp2gh 72 22 the the DT cord-022774-wasdp2gh 72 23 c c NN cord-022774-wasdp2gh 72 24 / / SYM cord-022774-wasdp2gh 72 25 s s NNP cord-022774-wasdp2gh 72 26 Golgi Golgi NNP cord-022774-wasdp2gh 72 27 apparatus apparatus NN cord-022774-wasdp2gh 72 28 cisternae cisternae NN cord-022774-wasdp2gh 72 29 and and CC cord-022774-wasdp2gh 72 30 the the DT cord-022774-wasdp2gh 72 31 transitional transitional JJ cord-022774-wasdp2gh 72 32 endoplasmie endoplasmie NNP cord-022774-wasdp2gh 72 33 reticulum reticulum NN cord-022774-wasdp2gh 72 34 . . . cord-022774-wasdp2gh 73 1 The the DT cord-022774-wasdp2gh 73 2 tubular tubular JJ cord-022774-wasdp2gh 73 3 transitional transitional JJ cord-022774-wasdp2gh 73 4 membranes membrane NNS cord-022774-wasdp2gh 73 5 were be VBD cord-022774-wasdp2gh 73 6 still still RB cord-022774-wasdp2gh 73 7 present present JJ cord-022774-wasdp2gh 73 8 at at IN cord-022774-wasdp2gh 73 9 both both CC cord-022774-wasdp2gh 73 10 16~C 16~c CD cord-022774-wasdp2gh 73 11 and and CC cord-022774-wasdp2gh 73 12 18 18 CD cord-022774-wasdp2gh 73 13 ° ° NN cord-022774-wasdp2gh 73 14 C c NN cord-022774-wasdp2gh 73 15 but but CC cord-022774-wasdp2gh 73 16 were be VBD cord-022774-wasdp2gh 73 17 less less RBR cord-022774-wasdp2gh 73 18 evident evident JJ cord-022774-wasdp2gh 73 19 than than IN cord-022774-wasdp2gh 73 20 at at IN cord-022774-wasdp2gh 73 21 8 8 CD cord-022774-wasdp2gh 73 22 ° ° NN cord-022774-wasdp2gh 73 23 C c NN cord-022774-wasdp2gh 73 24 or or CC cord-022774-wasdp2gh 73 25 12 12 CD cord-022774-wasdp2gh 73 26 ° ° NN cord-022774-wasdp2gh 73 27 C c NN cord-022774-wasdp2gh 73 28 . . . cord-022774-wasdp2gh 74 1 At at IN cord-022774-wasdp2gh 74 2 20 20 CD cord-022774-wasdp2gh 74 3 ° ° NN cord-022774-wasdp2gh 74 4 C c NN cord-022774-wasdp2gh 74 5 , , , cord-022774-wasdp2gh 74 6 the the DT cord-022774-wasdp2gh 74 7 appearance appearance NN cord-022774-wasdp2gh 74 8 of of IN cord-022774-wasdp2gh 74 9 the the DT cord-022774-wasdp2gh 74 10 Golgi Golgi NNP cord-022774-wasdp2gh 74 11 apparatus apparatus NN cord-022774-wasdp2gh 74 12 zone zone NN cord-022774-wasdp2gh 74 13 was be VBD cord-022774-wasdp2gh 74 14 changed change VBN cord-022774-wasdp2gh 74 15 markedly markedly RB cord-022774-wasdp2gh 74 16 from from IN cord-022774-wasdp2gh 74 17 that that DT cord-022774-wasdp2gh 74 18 at at IN cord-022774-wasdp2gh 74 19 16 16 CD cord-022774-wasdp2gh 74 20 ° ° NN cord-022774-wasdp2gh 74 21 C c NN cord-022774-wasdp2gh 74 22 or or CC cord-022774-wasdp2gh 74 23 18 18 CD cord-022774-wasdp2gh 74 24 ° ° NN cord-022774-wasdp2gh 74 25 C c NN cord-022774-wasdp2gh 74 26 ( ( -LRB- cord-022774-wasdp2gh 74 27 Fig Fig NNP cord-022774-wasdp2gh 74 28 . . . cord-022774-wasdp2gh 74 29 _SP cord-022774-wasdp2gh 74 30 3C 3C NNP cord-022774-wasdp2gh 74 31 and and CC cord-022774-wasdp2gh 74 32 4D 4D NNP cord-022774-wasdp2gh 74 33 ) ) -RRB- cord-022774-wasdp2gh 74 34 . . . cord-022774-wasdp2gh 75 1 The the DT cord-022774-wasdp2gh 75 2 number number NN cord-022774-wasdp2gh 75 3 of of IN cord-022774-wasdp2gh 75 4 transition transition NN cord-022774-wasdp2gh 75 5 vesicles vesicle NNS cord-022774-wasdp2gh 75 6 was be VBD cord-022774-wasdp2gh 75 7 reduced reduce VBN cord-022774-wasdp2gh 75 8 compared compare VBN cord-022774-wasdp2gh 75 9 to to IN cord-022774-wasdp2gh 75 10 18 18 CD cord-022774-wasdp2gh 75 11 ° ° NN cord-022774-wasdp2gh 75 12 C c NN cord-022774-wasdp2gh 75 13 and and CC cord-022774-wasdp2gh 75 14 those those DT cord-022774-wasdp2gh 75 15 vesicles vesicle NNS cord-022774-wasdp2gh 75 16 present present JJ cord-022774-wasdp2gh 75 17 were be VBD cord-022774-wasdp2gh 75 18 no no RB cord-022774-wasdp2gh 75 19 longer long RBR cord-022774-wasdp2gh 75 20 present present JJ cord-022774-wasdp2gh 75 21 in in IN cord-022774-wasdp2gh 75 22 clusters cluster NNS cord-022774-wasdp2gh 75 23 . . . cord-022774-wasdp2gh 76 1 Additionally additionally RB cord-022774-wasdp2gh 76 2 , , , cord-022774-wasdp2gh 76 3 the the DT cord-022774-wasdp2gh 76 4 tubular tubular JJ cord-022774-wasdp2gh 76 5 smooth smooth JJ cord-022774-wasdp2gh 76 6 membrane membrane NN cord-022774-wasdp2gh 76 7 elements element NNS cord-022774-wasdp2gh 76 8 present present JJ cord-022774-wasdp2gh 76 9 throughout throughout IN cord-022774-wasdp2gh 76 10 the the DT cord-022774-wasdp2gh 76 11 Golgi Golgi NNP cord-022774-wasdp2gh 76 12 apparatus apparatus NN cord-022774-wasdp2gh 76 13 zone zone NN cord-022774-wasdp2gh 76 14 at at IN cord-022774-wasdp2gh 76 15 temperatures temperature NNS cord-022774-wasdp2gh 76 16 of of IN cord-022774-wasdp2gh 76 17 18 18 CD cord-022774-wasdp2gh 76 18 ° ° NN cord-022774-wasdp2gh 76 19 C c NN cord-022774-wasdp2gh 76 20 and and CC cord-022774-wasdp2gh 76 21 below below RB cord-022774-wasdp2gh 76 22 were be VBD cord-022774-wasdp2gh 76 23 much much RB cord-022774-wasdp2gh 76 24 less less JJR cord-022774-wasdp2gh 76 25 in in IN cord-022774-wasdp2gh 76 26 evidence evidence NN cord-022774-wasdp2gh 76 27 . . . cord-022774-wasdp2gh 77 1 With with IN cord-022774-wasdp2gh 77 2 further further JJ cord-022774-wasdp2gh 77 3 increases increase NNS cord-022774-wasdp2gh 77 4 in in IN cord-022774-wasdp2gh 77 5 temperature temperature NN cord-022774-wasdp2gh 77 6 , , , cord-022774-wasdp2gh 77 7 the the DT cord-022774-wasdp2gh 77 8 numbers number NNS cord-022774-wasdp2gh 77 9 of of IN cord-022774-wasdp2gh 77 10 transition transition NN cord-022774-wasdp2gh 77 11 vesicles vesicle NNS cord-022774-wasdp2gh 77 12 appeared appear VBD cord-022774-wasdp2gh 77 13 to to TO cord-022774-wasdp2gh 77 14 be be VB cord-022774-wasdp2gh 77 15 increased increase VBN cord-022774-wasdp2gh 77 16 slightly slightly RB cord-022774-wasdp2gh 77 17 but but CC cord-022774-wasdp2gh 77 18 the the DT cord-022774-wasdp2gh 77 19 tubular tubular JJ cord-022774-wasdp2gh 77 20 elements element NNS cord-022774-wasdp2gh 77 21 of of IN cord-022774-wasdp2gh 77 22 the the DT cord-022774-wasdp2gh 77 23 endoplasmic endoplasmic NN cord-022774-wasdp2gh 77 24 reticulum reticulum NN cord-022774-wasdp2gh 77 25 did do VBD cord-022774-wasdp2gh 77 26 not not RB cord-022774-wasdp2gh 77 27 reappear reappear VB cord-022774-wasdp2gh 77 28 within within IN cord-022774-wasdp2gh 77 29 the the DT cord-022774-wasdp2gh 77 30 Golgi Golgi NNP cord-022774-wasdp2gh 77 31 apparatus apparatus NN cord-022774-wasdp2gh 77 32 zone zone NN cord-022774-wasdp2gh 77 33 . . . cord-022774-wasdp2gh 78 1 Overall overall RB cord-022774-wasdp2gh 78 2 , , , cord-022774-wasdp2gh 78 3 the the DT cord-022774-wasdp2gh 78 4 numbers number NNS cord-022774-wasdp2gh 78 5 of of IN cord-022774-wasdp2gh 78 6 transition transition NN cord-022774-wasdp2gh 78 7 vesicles vesicle NNS cord-022774-wasdp2gh 78 8 present present JJ cord-022774-wasdp2gh 78 9 at at IN cord-022774-wasdp2gh 78 10 the the DT cord-022774-wasdp2gh 78 11 cis cis NNP cord-022774-wasdp2gh 78 12 Golgi Golgi NNP cord-022774-wasdp2gh 78 13 apparatus apparatus NN cord-022774-wasdp2gh 78 14 face face NN cord-022774-wasdp2gh 78 15 showed show VBD cord-022774-wasdp2gh 78 16 an an DT cord-022774-wasdp2gh 78 17 increase increase NN cord-022774-wasdp2gh 78 18 with with IN cord-022774-wasdp2gh 78 19 increasing increase VBG cord-022774-wasdp2gh 78 20 temperature temperature NN cord-022774-wasdp2gh 78 21 to to IN cord-022774-wasdp2gh 78 22 an an DT cord-022774-wasdp2gh 78 23 optimum optimum NN cord-022774-wasdp2gh 78 24 at at IN cord-022774-wasdp2gh 78 25 16 16 CD cord-022774-wasdp2gh 78 26 ° ° NN cord-022774-wasdp2gh 78 27 C c NN cord-022774-wasdp2gh 78 28 ( ( -LRB- cord-022774-wasdp2gh 78 29 30 30 CD cord-022774-wasdp2gh 78 30 rain rain NN cord-022774-wasdp2gh 78 31 ) ) -RRB- cord-022774-wasdp2gh 78 32 or or CC cord-022774-wasdp2gh 78 33 18 18 CD cord-022774-wasdp2gh 78 34 ° ° NN cord-022774-wasdp2gh 78 35 C c NN cord-022774-wasdp2gh 78 36 ( ( -LRB- cord-022774-wasdp2gh 78 37 60 60 CD cord-022774-wasdp2gh 78 38 rain rain NN cord-022774-wasdp2gh 78 39 ) ) -RRB- cord-022774-wasdp2gh 78 40 with with IN cord-022774-wasdp2gh 78 41 an an DT cord-022774-wasdp2gh 78 42 abrupt abrupt JJ cord-022774-wasdp2gh 78 43 decline decline NN cord-022774-wasdp2gh 78 44 in in IN cord-022774-wasdp2gh 78 45 transition transition NN cord-022774-wasdp2gh 78 46 vesicle vesicle NN cord-022774-wasdp2gh 78 47 numbers number NNS cord-022774-wasdp2gh 78 48 between between IN cord-022774-wasdp2gh 78 49 18 18 CD cord-022774-wasdp2gh 78 50 ° ° NN cord-022774-wasdp2gh 78 51 C c NN cord-022774-wasdp2gh 78 52 and and CC cord-022774-wasdp2gh 78 53 20 20 CD cord-022774-wasdp2gh 78 54 ° ° NN cord-022774-wasdp2gh 78 55 C c NN cord-022774-wasdp2gh 78 56 ( ( -LRB- cord-022774-wasdp2gh 78 57 Fig Fig NNP cord-022774-wasdp2gh 78 58 . . . cord-022774-wasdp2gh 78 59 5 5 CD cord-022774-wasdp2gh 78 60 ) ) -RRB- cord-022774-wasdp2gh 78 61 . . . cord-022774-wasdp2gh 79 1 As as IN cord-022774-wasdp2gh 79 2 with with IN cord-022774-wasdp2gh 79 3 the the DT cord-022774-wasdp2gh 79 4 isolated isolate VBN cord-022774-wasdp2gh 79 5 fractions fraction NNS cord-022774-wasdp2gh 79 6 ( ( -LRB- cord-022774-wasdp2gh 79 7 Fig fig NN cord-022774-wasdp2gh 79 8 . . NNP cord-022774-wasdp2gh 79 9 2 2 CD cord-022774-wasdp2gh 79 10 ) ) -RRB- cord-022774-wasdp2gh 79 11 , , , cord-022774-wasdp2gh 79 12 the the DT cord-022774-wasdp2gh 79 13 numbers number NNS cord-022774-wasdp2gh 79 14 of of IN cord-022774-wasdp2gh 79 15 transition transition NN cord-022774-wasdp2gh 79 16 vesicles vesicle NNS cord-022774-wasdp2gh 79 17 did do VBD cord-022774-wasdp2gh 79 18 not not RB cord-022774-wasdp2gh 79 19 appear appear VB cord-022774-wasdp2gh 79 20 to to TO cord-022774-wasdp2gh 79 21 increase increase VB cord-022774-wasdp2gh 79 22 until until IN cord-022774-wasdp2gh 79 23 temperatures temperature NNS cord-022774-wasdp2gh 79 24 exceeded exceed VBD cord-022774-wasdp2gh 79 25 12 12 CD cord-022774-wasdp2gh 79 26 ° ° NN cord-022774-wasdp2gh 79 27 C c NN cord-022774-wasdp2gh 79 28 . . . cord-022774-wasdp2gh 80 1 Neither neither CC cord-022774-wasdp2gh 80 2 did do VBD cord-022774-wasdp2gh 80 3 they -PRON- PRP cord-022774-wasdp2gh 80 4 accumulate accumulate VB cord-022774-wasdp2gh 80 5 in in IN cord-022774-wasdp2gh 80 6 large large JJ cord-022774-wasdp2gh 80 7 numbers number NNS cord-022774-wasdp2gh 80 8 at at IN cord-022774-wasdp2gh 80 9 temperatures temperature NNS cord-022774-wasdp2gh 80 10 above above IN cord-022774-wasdp2gh 80 11 18 18 CD cord-022774-wasdp2gh 80 12 ° ° NN cord-022774-wasdp2gh 80 13 C c NN cord-022774-wasdp2gh 80 14 presumably presumably RB cord-022774-wasdp2gh 80 15 through through IN cord-022774-wasdp2gh 80 16 release release NN cord-022774-wasdp2gh 80 17 of of IN cord-022774-wasdp2gh 80 18 the the DT cord-022774-wasdp2gh 80 19 temperature temperature NN cord-022774-wasdp2gh 80 20 block block NN cord-022774-wasdp2gh 80 21 at at IN cord-022774-wasdp2gh 80 22 temperatures temperature NNS cord-022774-wasdp2gh 80 23 below below IN cord-022774-wasdp2gh 80 24 18 18 CD cord-022774-wasdp2gh 80 25 ° ° NN cord-022774-wasdp2gh 80 26 C c NN cord-022774-wasdp2gh 80 27 that that WDT cord-022774-wasdp2gh 80 28 allowed allow VBD cord-022774-wasdp2gh 80 29 for for IN cord-022774-wasdp2gh 80 30 their -PRON- PRP$ cord-022774-wasdp2gh 80 31 formation formation NN cord-022774-wasdp2gh 80 32 but but CC cord-022774-wasdp2gh 80 33 retarded retard VBD cord-022774-wasdp2gh 80 34 their -PRON- PRP$ cord-022774-wasdp2gh 80 35 fusion fusion NN cord-022774-wasdp2gh 80 36 with with IN cord-022774-wasdp2gh 80 37 the the DT cord-022774-wasdp2gh 80 38 Golgi Golgi NNP cord-022774-wasdp2gh 80 39 apparatus apparatus NN cord-022774-wasdp2gh 80 40 . . . cord-022774-wasdp2gh 81 1 The the DT cord-022774-wasdp2gh 81 2 distribution distribution NN cord-022774-wasdp2gh 81 3 of of IN cord-022774-wasdp2gh 81 4 diameters diameter NNS cord-022774-wasdp2gh 81 5 of of IN cord-022774-wasdp2gh 81 6 transition transition NN cord-022774-wasdp2gh 81 7 vesicles vesicle NNS cord-022774-wasdp2gh 81 8 present present JJ cord-022774-wasdp2gh 81 9 in in IN cord-022774-wasdp2gh 81 10 situ situ NN cord-022774-wasdp2gh 81 11 at at IN cord-022774-wasdp2gh 81 12 4 4 CD cord-022774-wasdp2gh 81 13 ° ° NN cord-022774-wasdp2gh 81 14 C C NNP cord-022774-wasdp2gh 81 15 differed differ VBD cord-022774-wasdp2gh 81 16 from from IN cord-022774-wasdp2gh 81 17 those those DT cord-022774-wasdp2gh 81 18 present present JJ cord-022774-wasdp2gh 81 19 in in IN cord-022774-wasdp2gh 81 20 vitro vitro FW cord-022774-wasdp2gh 81 21 with with IN cord-022774-wasdp2gh 81 22 isolated isolate VBN cord-022774-wasdp2gh 81 23 transition transition NN cord-022774-wasdp2gh 81 24 elements element NNS cord-022774-wasdp2gh 81 25 at at IN cord-022774-wasdp2gh 81 26 4 4 CD cord-022774-wasdp2gh 81 27 ° ° NN cord-022774-wasdp2gh 81 28 C c NN cord-022774-wasdp2gh 81 29 ( ( -LRB- cord-022774-wasdp2gh 81 30 Fig Fig NNP cord-022774-wasdp2gh 81 31 . . . cord-022774-wasdp2gh 81 32 6 6 CD cord-022774-wasdp2gh 81 33 ) ) -RRB- cord-022774-wasdp2gh 81 34 . . . cord-022774-wasdp2gh 82 1 Those those DT cord-022774-wasdp2gh 82 2 formed form VBN cord-022774-wasdp2gh 82 3 in in IN cord-022774-wasdp2gh 82 4 vitro vitro FW cord-022774-wasdp2gh 82 5 exhibited exhibit VBD cord-022774-wasdp2gh 82 6 a a DT cord-022774-wasdp2gh 82 7 larger large JJR cord-022774-wasdp2gh 82 8 diameter diameter NN cord-022774-wasdp2gh 82 9 of of IN cord-022774-wasdp2gh 82 10 72/~m 72/~m NN cord-022774-wasdp2gh 82 11 compared compare VBN cord-022774-wasdp2gh 82 12 to to IN cord-022774-wasdp2gh 82 13 55 55 CD cord-022774-wasdp2gh 83 1 ~m ~m JJ cord-022774-wasdp2gh 84 1 in in IN cord-022774-wasdp2gh 84 2 ~itu ~itu NNP cord-022774-wasdp2gh 84 3 . . . cord-022774-wasdp2gh 85 1 Comparing compare VBG cord-022774-wasdp2gh 85 2 vesicles vesicle NNS cord-022774-wasdp2gh 85 3 formed form VBN cord-022774-wasdp2gh 85 4 in in IN cord-022774-wasdp2gh 85 5 situ situ NN cord-022774-wasdp2gh 85 6 at at IN cord-022774-wasdp2gh 85 7 37 37 CD cord-022774-wasdp2gh 85 8 ° ° NN cord-022774-wasdp2gh 85 9 C c NN cord-022774-wasdp2gh 85 10 with with IN cord-022774-wasdp2gh 85 11 those those DT cord-022774-wasdp2gh 85 12 formed form VBN cord-022774-wasdp2gh 85 13 at at IN cord-022774-wasdp2gh 85 14 40C 40c CD cord-022774-wasdp2gh 85 15 ( ( -LRB- cord-022774-wasdp2gh 85 16 Fig Fig NNP cord-022774-wasdp2gh 85 17 . . . cord-022774-wasdp2gh 85 18 6 6 CD cord-022774-wasdp2gh 85 19 ) ) -RRB- cord-022774-wasdp2gh 85 20 , , , cord-022774-wasdp2gh 85 21 the the DT cord-022774-wasdp2gh 85 22 diameters diameter NNS cord-022774-wasdp2gh 85 23 ~so ~so NNP cord-022774-wasdp2gh 85 24 were be VBD cord-022774-wasdp2gh 85 25 shifted shift VBN cord-022774-wasdp2gh 85 26 to to IN cord-022774-wasdp2gh 85 27 larger large JJR cord-022774-wasdp2gh 85 28 values value NNS cord-022774-wasdp2gh 85 29 in in IN cord-022774-wasdp2gh 85 30 the the DT cord-022774-wasdp2gh 85 31 distribution distribution NN cord-022774-wasdp2gh 85 32 profile profile NN cord-022774-wasdp2gh 85 33 , , , cord-022774-wasdp2gh 85 34 ~.z ~.z NNP cord-022774-wasdp2gh 85 35 ~ ~ NFP cord-022774-wasdp2gh 85 36 d d XX cord-022774-wasdp2gh 85 37 the the DT cord-022774-wasdp2gh 85 38 average average JJ cord-022774-wasdp2gh 85 39 diameter diameter NN cord-022774-wasdp2gh 85 40 was be VBD cord-022774-wasdp2gh 85 41 increased increase VBN cord-022774-wasdp2gh 85 42 to to IN cord-022774-wasdp2gh 85 43 65/~m 65/~m NNP cord-022774-wasdp2gh 85 44 . . . cord-022774-wasdp2gh 86 1 A a DT cord-022774-wasdp2gh 86 2 similar similar JJ cord-022774-wasdp2gh 86 3 increase increase NN cord-022774-wasdp2gh 86 4 in in IN cord-022774-wasdp2gh 86 5 vesicle vesicle NN cord-022774-wasdp2gh 86 6 diameter diameter NN cord-022774-wasdp2gh 86 7 was be VBD cord-022774-wasdp2gh 86 8 seen see VBN cord-022774-wasdp2gh 86 9 with with IN cord-022774-wasdp2gh 86 10 the the DT cord-022774-wasdp2gh 86 11 transition transition NN cord-022774-wasdp2gh 86 12 vesicle vesicle NN cord-022774-wasdp2gh 86 13 formed form VBN cord-022774-wasdp2gh 86 14 in in IN cord-022774-wasdp2gh 86 15 vitro vitro FW cord-022774-wasdp2gh 86 16 from from IN cord-022774-wasdp2gh 86 17 73/~m 73/~m NNP cord-022774-wasdp2gh 86 18 at at IN cord-022774-wasdp2gh 86 19 4 4 CD cord-022774-wasdp2gh 86 20 ° ° NN cord-022774-wasdp2gh 86 21 C c NN cord-022774-wasdp2gh 86 22 to to IN cord-022774-wasdp2gh 86 23 88/~m 88/~m NN cord-022774-wasdp2gh 86 24 at at IN cord-022774-wasdp2gh 86 25 37 37 CD cord-022774-wasdp2gh 86 26 ° ° NN cord-022774-wasdp2gh 86 27 C c NN cord-022774-wasdp2gh 86 28 . . . cord-022774-wasdp2gh 87 1 Since since IN cord-022774-wasdp2gh 87 2 most most JJS cord-022774-wasdp2gh 87 3 previous previous JJ cord-022774-wasdp2gh 87 4 investigations investigation NNS cord-022774-wasdp2gh 87 5 of of IN cord-022774-wasdp2gh 87 6 temperature temperature NN cord-022774-wasdp2gh 87 7 effects effect NNS cord-022774-wasdp2gh 87 8 on on IN cord-022774-wasdp2gh 87 9 transition~ transition~ NNP cord-022774-wasdp2gh 87 10 endoplasmic endoplasmic JJ cord-022774-wasdp2gh 87 11 reticulum reticulum NN cord-022774-wasdp2gh 87 12 have have VBP cord-022774-wasdp2gh 87 13 been be VBN cord-022774-wasdp2gh 87 14 with with IN cord-022774-wasdp2gh 87 15 cultured cultured JJ cord-022774-wasdp2gh 87 16 cells cell NNS cord-022774-wasdp2gh 87 17 , , , cord-022774-wasdp2gh 87 18 studies study NNS cord-022774-wasdp2gh 87 19 were be VBD cord-022774-wasdp2gh 87 20 carried carry VBN cord-022774-wasdp2gh 87 21 out out RP cord-022774-wasdp2gh 87 22 with with IN cord-022774-wasdp2gh 87 23 BHK BHK NNP cord-022774-wasdp2gh 87 24 cells cell NNS cord-022774-wasdp2gh 87 25 under under IN cord-022774-wasdp2gh 87 26 identical identical JJ cord-022774-wasdp2gh 87 27 conditions condition NNS cord-022774-wasdp2gh 87 28 of of IN cord-022774-wasdp2gh 87 29 temperature temperature NN cord-022774-wasdp2gh 87 30 incubations incubation NNS cord-022774-wasdp2gh 87 31 used use VBN cord-022774-wasdp2gh 87 32 for for IN cord-022774-wasdp2gh 87 33 liver liver NN cord-022774-wasdp2gh 87 34 slices slice NNS cord-022774-wasdp2gh 87 35 . . . cord-022774-wasdp2gh 88 1 At at IN cord-022774-wasdp2gh 88 2 16 16 CD cord-022774-wasdp2gh 88 3 ° ° NN cord-022774-wasdp2gh 88 4 C c NN cord-022774-wasdp2gh 88 5 ( ( -LRB- cord-022774-wasdp2gh 88 6 Fig Fig NNP cord-022774-wasdp2gh 88 7 . . . cord-022774-wasdp2gh 88 8 7A 7a NN cord-022774-wasdp2gh 88 9 ) ) -RRB- cord-022774-wasdp2gh 88 10 , , , cord-022774-wasdp2gh 88 11 the the DT cord-022774-wasdp2gh 88 12 general general JJ cord-022774-wasdp2gh 88 13 appearance appearance NN cord-022774-wasdp2gh 89 1 The the DT cord-022774-wasdp2gh 89 2 use use NN cord-022774-wasdp2gh 89 3 of of IN cord-022774-wasdp2gh 89 4 inhibitors inhibitor NNS cord-022774-wasdp2gh 89 5 to to TO cord-022774-wasdp2gh 89 6 interrupt interrupt VB cord-022774-wasdp2gh 89 7 the the DT cord-022774-wasdp2gh 89 8 flow flow NN cord-022774-wasdp2gh 89 9 of of IN cord-022774-wasdp2gh 89 10 membrane~ membrane~ NNS cord-022774-wasdp2gh 89 11 between between IN cord-022774-wasdp2gh 89 12 the the DT cord-022774-wasdp2gh 89 13 endoplasmie endoplasmie NNP cord-022774-wasdp2gh 89 14 reticulum reticulum NN cord-022774-wasdp2gh 89 15 and and CC cord-022774-wasdp2gh 89 16 the the DT cord-022774-wasdp2gh 89 17 Golgi Golgi NNP cord-022774-wasdp2gh 89 18 apparatus apparatus NN cord-022774-wasdp2gh 89 19 might may MD cord-022774-wasdp2gh 89 20 be be VB cord-022774-wasdp2gh 89 21 expected expect VBN cord-022774-wasdp2gh 89 22 to to TO cord-022774-wasdp2gh 89 23 have have VB cord-022774-wasdp2gh 89 24 effects effect NNS cord-022774-wasdp2gh 89 25 similar similar JJ cord-022774-wasdp2gh 89 26 to to IN cord-022774-wasdp2gh 89 27 low low JJ cord-022774-wasdp2gh 89 28 temperatures temperature NNS cord-022774-wasdp2gh 89 29 . . . cord-022774-wasdp2gh 90 1 Therefore therefore RB cord-022774-wasdp2gh 90 2 , , , cord-022774-wasdp2gh 90 3 BHK BHK NNP cord-022774-wasdp2gh 90 4 cells cell NNS cord-022774-wasdp2gh 90 5 were be VBD cord-022774-wasdp2gh 90 6 treated treat VBN cord-022774-wasdp2gh 90 7 with with IN cord-022774-wasdp2gh 90 8 substances substance NNS cord-022774-wasdp2gh 90 9 to to TO cord-022774-wasdp2gh 90 10 block block VB cord-022774-wasdp2gh 90 11 membrane membrane NN cord-022774-wasdp2gh 90 12 flow flow NN cord-022774-wasdp2gh 90 13 . . . cord-022774-wasdp2gh 91 1 Potassium potassium NN cord-022774-wasdp2gh 91 2 cyanide cyanide NN cord-022774-wasdp2gh 91 3 blocks block VBZ cord-022774-wasdp2gh 91 4 secretion secretion NN cord-022774-wasdp2gh 91 5 , , , cord-022774-wasdp2gh 91 6 presumably presumably RB cord-022774-wasdp2gh 91 7 by by IN cord-022774-wasdp2gh 91 8 reducing reduce VBG cord-022774-wasdp2gh 91 9 the the DT cord-022774-wasdp2gh 91 10 cellular cellular JJ cord-022774-wasdp2gh 91 11 energy energy NN cord-022774-wasdp2gh 91 12 charge charge NN cord-022774-wasdp2gh 91 13 , , , cord-022774-wasdp2gh 91 14 but but CC cord-022774-wasdp2gh 91 15 did do VBD cord-022774-wasdp2gh 91 16 not not RB cord-022774-wasdp2gh 91 17 result result VB cord-022774-wasdp2gh 91 18 in in IN cord-022774-wasdp2gh 91 19 an an DT cord-022774-wasdp2gh 91 20 accumulation accumulation NN cord-022774-wasdp2gh 91 21 of of IN cord-022774-wasdp2gh 91 22 transition transition NN cord-022774-wasdp2gh 91 23 vesicles vesicle NNS cord-022774-wasdp2gh 91 24 ( ( -LRB- cord-022774-wasdp2gh 91 25 Fig Fig NNP cord-022774-wasdp2gh 91 26 . . . cord-022774-wasdp2gh 91 27 9B 9b LS cord-022774-wasdp2gh 91 28 ) ) -RRB- cord-022774-wasdp2gh 91 29 compared compare VBN cord-022774-wasdp2gh 91 30 with with IN cord-022774-wasdp2gh 91 31 control control NN cord-022774-wasdp2gh 91 32 preparations preparation NNS cord-022774-wasdp2gh 91 33 ( ( -LRB- cord-022774-wasdp2gh 91 34 Fig Fig NNP cord-022774-wasdp2gh 91 35 . . . cord-022774-wasdp2gh 91 36 9A 9a NN cord-022774-wasdp2gh 91 37 ) ) -RRB- cord-022774-wasdp2gh 91 38 with with IN cord-022774-wasdp2gh 91 39 15 15 CD cord-022774-wasdp2gh 91 40 , , , cord-022774-wasdp2gh 91 41 30 30 CD cord-022774-wasdp2gh 91 42 or or CC cord-022774-wasdp2gh 91 43 60 60 CD cord-022774-wasdp2gh 91 44 rain rain NN cord-022774-wasdp2gh 91 45 of of IN cord-022774-wasdp2gh 91 46 incubation incubation NN cord-022774-wasdp2gh 91 47 . . . cord-022774-wasdp2gh 92 1 In in IN cord-022774-wasdp2gh 92 2 contrast contrast NN cord-022774-wasdp2gh 92 3 , , , cord-022774-wasdp2gh 92 4 1 1 CD cord-022774-wasdp2gh 92 5 mM mm CD cord-022774-wasdp2gh 92 6 CoC CoC NNP cord-022774-wasdp2gh 92 7 ! ! . cord-022774-wasdp2gh 92 8 2 2 CD cord-022774-wasdp2gh 92 9 conditions condition NNS cord-022774-wasdp2gh 92 10 known know VBN cord-022774-wasdp2gh 92 11 to to TO cord-022774-wasdp2gh 92 12 block block VB cord-022774-wasdp2gh 92 13 vesicular vesicular JJ cord-022774-wasdp2gh 92 14 flow flow NN cord-022774-wasdp2gh 92 15 in in IN cord-022774-wasdp2gh 92 16 other other JJ cord-022774-wasdp2gh 92 17 systems system NNS cord-022774-wasdp2gh 92 18 and and CC cord-022774-wasdp2gh 92 19 axonal axonal JJ cord-022774-wasdp2gh 92 20 transport transport NN cord-022774-wasdp2gh 92 21 ( ( -LRB- cord-022774-wasdp2gh 92 22 see see VB cord-022774-wasdp2gh 92 23 Discussion Discussion NNP cord-022774-wasdp2gh 92 24 ) ) -RRB- cord-022774-wasdp2gh 92 25 , , , cord-022774-wasdp2gh 92 26 did do VBD cord-022774-wasdp2gh 92 27 produce produce VB cord-022774-wasdp2gh 92 28 morphological morphological JJ cord-022774-wasdp2gh 92 29 changes change NNS cord-022774-wasdp2gh 92 30 very very RB cord-022774-wasdp2gh 92 31 similar similar JJ cord-022774-wasdp2gh 92 32 to to IN cord-022774-wasdp2gh 92 33 those those DT cord-022774-wasdp2gh 92 34 observed observe VBN cord-022774-wasdp2gh 92 35 with with IN cord-022774-wasdp2gh 92 36 the the DT cord-022774-wasdp2gh 92 37 low low JJ cord-022774-wasdp2gh 92 38 temperature temperature NN cord-022774-wasdp2gh 92 39 block block NN cord-022774-wasdp2gh 92 40 ( ( -LRB- cord-022774-wasdp2gh 92 41 Fig Fig NNP cord-022774-wasdp2gh 92 42 . . . cord-022774-wasdp2gh 92 43 9C 9c CD cord-022774-wasdp2gh 92 44 ) ) -RRB- cord-022774-wasdp2gh 92 45 . . . cord-022774-wasdp2gh 93 1 Numbers number NNS cord-022774-wasdp2gh 93 2 of of IN cord-022774-wasdp2gh 93 3 transition transition NN cord-022774-wasdp2gh 93 4 vesicles vesicle NNS cord-022774-wasdp2gh 93 5 were be VBD cord-022774-wasdp2gh 93 6 approximately approximately RB cord-022774-wasdp2gh 93 7 doubled double VBN cord-022774-wasdp2gh 93 8 relative relative JJ cord-022774-wasdp2gh 93 9 to to IN cord-022774-wasdp2gh 93 10 the the DT cord-022774-wasdp2gh 93 11 control control NN cord-022774-wasdp2gh 93 12 preparations preparation NNS cord-022774-wasdp2gh 93 13 and and CC cord-022774-wasdp2gh 93 14 the the DT cord-022774-wasdp2gh 93 15 peripheral peripheral JJ cord-022774-wasdp2gh 93 16 cytoplasm cytoplasm NN cord-022774-wasdp2gh 93 17 contained contain VBD cord-022774-wasdp2gh 93 18 numerous numerous JJ cord-022774-wasdp2gh 93 19 tubular tubular JJ cord-022774-wasdp2gh 93 20 transition transition NN cord-022774-wasdp2gh 93 21 elements element NNS cord-022774-wasdp2gh 93 22 of of IN cord-022774-wasdp2gh 93 23 the the DT cord-022774-wasdp2gh 93 24 endoplasmic endoplasmic NN cord-022774-wasdp2gh 93 25 reticulum reticulum NN cord-022774-wasdp2gh 93 26 similar similar JJ cord-022774-wasdp2gh 93 27 in in IN cord-022774-wasdp2gh 93 28 appearance appearance NN cord-022774-wasdp2gh 93 29 to to IN cord-022774-wasdp2gh 93 30 those those DT cord-022774-wasdp2gh 93 31 observed observe VBN cord-022774-wasdp2gh 93 32 with with IN cord-022774-wasdp2gh 93 33 incubation incubation NN cord-022774-wasdp2gh 93 34 at at IN cord-022774-wasdp2gh 93 35 16 16 CD cord-022774-wasdp2gh 93 36 ° ° NN cord-022774-wasdp2gh 93 37 C c NN cord-022774-wasdp2gh 93 38 ( ( -LRB- cord-022774-wasdp2gh 93 39 Fig Fig NNP cord-022774-wasdp2gh 93 40 . . . cord-022774-wasdp2gh 93 41 _SP cord-022774-wasdp2gh 93 42 7A 7a NN cord-022774-wasdp2gh 93 43 ) ) -RRB- cord-022774-wasdp2gh 93 44 . . . cord-022774-wasdp2gh 94 1 These these DT cord-022774-wasdp2gh 94 2 morphological morphological JJ cord-022774-wasdp2gh 94 3 changes change NNS cord-022774-wasdp2gh 94 4 were be VBD cord-022774-wasdp2gh 94 5 observed observe VBN cord-022774-wasdp2gh 94 6 with with IN cord-022774-wasdp2gh 94 7 incubation incubation NN cord-022774-wasdp2gh 94 8 times time NNS cord-022774-wasdp2gh 94 9 with with IN cord-022774-wasdp2gh 94 10 1 1 CD cord-022774-wasdp2gh 94 11 mM mm CD cord-022774-wasdp2gh 94 12 CoCI CoCI NNP cord-022774-wasdp2gh 94 13 2 2 CD cord-022774-wasdp2gh 94 14 as as RB cord-022774-wasdp2gh 94 15 short short JJ cord-022774-wasdp2gh 94 16 as as IN cord-022774-wasdp2gh 94 17 15 15 CD cord-022774-wasdp2gh 94 18 rain rain NN cord-022774-wasdp2gh 94 19 and and CC cord-022774-wasdp2gh 94 20 the the DT cord-022774-wasdp2gh 94 21 alterations alteration NNS cord-022774-wasdp2gh 94 22 after after IN cord-022774-wasdp2gh 94 23 60 60 CD cord-022774-wasdp2gh 94 24 rain rain NN cord-022774-wasdp2gh 94 25 ( ( -LRB- cord-022774-wasdp2gh 94 26 not not RB cord-022774-wasdp2gh 94 27 illustrated illustrate VBN cord-022774-wasdp2gh 94 28 ) ) -RRB- cord-022774-wasdp2gh 94 29 of of IN cord-022774-wasdp2gh 94 30 incubation incubation NN cord-022774-wasdp2gh 94 31 were be VBD cord-022774-wasdp2gh 94 32 similar similar JJ cord-022774-wasdp2gh 94 33 to to IN cord-022774-wasdp2gh 94 34 those those DT cord-022774-wasdp2gh 94 35 after after IN cord-022774-wasdp2gh 94 36 30 30 CD cord-022774-wasdp2gh 94 37 rain rain NN cord-022774-wasdp2gh 94 38 of of IN cord-022774-wasdp2gh 94 39 inct inct NN cord-022774-wasdp2gh 94 40 : : : cord-022774-wasdp2gh 94 41 bation bation NN cord-022774-wasdp2gh 94 42 ( ( -LRB- cord-022774-wasdp2gh 94 43 Fig Fig NNP cord-022774-wasdp2gh 94 44 . . NNP cord-022774-wasdp2gh 94 45 6 6 CD cord-022774-wasdp2gh 94 46 , , , cord-022774-wasdp2gh 94 47 _SP cord-022774-wasdp2gh 94 48 Fig Fig NNP cord-022774-wasdp2gh 94 49 . . . cord-022774-wasdp2gh 94 50 9C 9c CD cord-022774-wasdp2gh 94 51 ) ) -RRB- cord-022774-wasdp2gh 94 52 . . . cord-022774-wasdp2gh 95 1 Using use VBG cord-022774-wasdp2gh 95 2 part part NN cord-022774-wasdp2gh 95 3 - - HYPH cord-022774-wasdp2gh 95 4 rough rough JJ cord-022774-wasdp2gh 95 5 , , , cord-022774-wasdp2gh 95 6 part part NN cord-022774-wasdp2gh 95 7 - - HYPH cord-022774-wasdp2gh 95 8 smooth smooth JJ cord-022774-wasdp2gh 95 9 transitional transitional JJ cord-022774-wasdp2gh 95 10 element element NN cord-022774-wasdp2gh 95 11 fractions fraction NNS cord-022774-wasdp2gh 95 12 isolated isolate VBN cord-022774-wasdp2gh 95 13 from from IN cord-022774-wasdp2gh 95 14 rat rat NN cord-022774-wasdp2gh 95 15 liver liver NN cord-022774-wasdp2gh 95 16 , , , cord-022774-wasdp2gh 95 17 Nowack Nowack NNP cord-022774-wasdp2gh 95 18 et et FW cord-022774-wasdp2gh 95 19 al al NNP cord-022774-wasdp2gh 95 20 . . . cord-022774-wasdp2gh 96 1 [ [ -LRB- cord-022774-wasdp2gh 96 2 15 15 CD cord-022774-wasdp2gh 96 3 ] ] -RRB- cord-022774-wasdp2gh 96 4 demonstrated demonstrate VBD cord-022774-wasdp2gh 96 5 the the DT cord-022774-wasdp2gh 96 6 temperature temperature NN cord-022774-wasdp2gh 96 7 dependent dependent JJ cord-022774-wasdp2gh 96 8 transfer transfer NN cord-022774-wasdp2gh 96 9 of of IN cord-022774-wasdp2gh 96 10 mereof mereof NNP cord-022774-wasdp2gh 96 11 the the DT cord-022774-wasdp2gh 96 12 Golgi Golgi NNP cord-022774-wasdp2gh 96 13 apparatus apparatus NN cord-022774-wasdp2gh 96 14 zone zone NN cord-022774-wasdp2gh 96 15 of of IN cord-022774-wasdp2gh 96 16 the the DT cord-022774-wasdp2gh 96 17 BHK BHK NNP cord-022774-wasdp2gh 96 18 cells cell NNS cord-022774-wasdp2gh 96 19 was be VBD cord-022774-wasdp2gh 96 20 similar similar JJ cord-022774-wasdp2gh 96 21 to to IN cord-022774-wasdp2gh 96 22 that that DT cord-022774-wasdp2gh 96 23 for for IN cord-022774-wasdp2gh 96 24 liver liver NN cord-022774-wasdp2gh 96 25 . . . cord-022774-wasdp2gh 97 1 Transition transition NN cord-022774-wasdp2gh 97 2 vesicles vesicle NNS cord-022774-wasdp2gh 97 3 present present JJ cord-022774-wasdp2gh 97 4 in in IN cord-022774-wasdp2gh 98 1 the the DT cord-022774-wasdp2gh 98 2 cytoplasm cytoplasm NN cord-022774-wasdp2gh 98 3 adjacent adjacent JJ cord-022774-wasdp2gh 98 4 to to IN cord-022774-wasdp2gh 98 5 the the DT cord-022774-wasdp2gh 98 6 cis cis NNP cord-022774-wasdp2gh 98 7 Golgi Golgi NNP cord-022774-wasdp2gh 98 8 apparatus apparatus NN cord-022774-wasdp2gh 98 9 face face NN cord-022774-wasdp2gh 98 10 were be VBD cord-022774-wasdp2gh 98 11 numerous numerous JJ cord-022774-wasdp2gh 98 12 and and CC cord-022774-wasdp2gh 98 13 accompanied accompany VBN cord-022774-wasdp2gh 98 14 by by IN cord-022774-wasdp2gh 98 15 abundant abundant JJ cord-022774-wasdp2gh 98 16 tubular tubular JJ cord-022774-wasdp2gh 98 17 transitional transitional JJ cord-022774-wasdp2gh 98 18 elements element NNS cord-022774-wasdp2gh 98 19 in in IN cord-022774-wasdp2gh 98 20 the the DT cord-022774-wasdp2gh 98 21 peripheral peripheral JJ cord-022774-wasdp2gh 98 22 cytoplasm cytoplasm NN cord-022774-wasdp2gh 98 23 . . . cord-022774-wasdp2gh 99 1 With with IN cord-022774-wasdp2gh 99 2 cells cell NNS cord-022774-wasdp2gh 99 3 incubated incubate VBN cord-022774-wasdp2gh 99 4 at at IN cord-022774-wasdp2gh 99 5 20 20 CD cord-022774-wasdp2gh 99 6 ° ° NN cord-022774-wasdp2gh 99 7 C c NN cord-022774-wasdp2gh 99 8 , , , cord-022774-wasdp2gh 99 9 both both CC cord-022774-wasdp2gh 99 10 the the DT cord-022774-wasdp2gh 99 11 tubular tubular JJ cord-022774-wasdp2gh 99 12 transitional transitional JJ cord-022774-wasdp2gh 99 13 elements element NNS cord-022774-wasdp2gh 99 14 of of IN cord-022774-wasdp2gh 99 15 the the DT cord-022774-wasdp2gh 99 16 endoplasmic endoplasmic NN cord-022774-wasdp2gh 99 17 reticulum reticulum NN cord-022774-wasdp2gh 99 18 and and CC cord-022774-wasdp2gh 99 19 the the DT cord-022774-wasdp2gh 99 20 numbers number NNS cord-022774-wasdp2gh 99 21 of of IN cord-022774-wasdp2gh 99 22 transition transition NN cord-022774-wasdp2gh 99 23 vesicles vesicle NNS cord-022774-wasdp2gh 99 24 were be VBD cord-022774-wasdp2gh 99 25 reduced reduce VBN cord-022774-wasdp2gh 99 26 markedly markedly RB cord-022774-wasdp2gh 99 27 ( ( -LRB- cord-022774-wasdp2gh 99 28 Fig Fig NNP cord-022774-wasdp2gh 99 29 . . . cord-022774-wasdp2gh 99 30 7B 7B NNP cord-022774-wasdp2gh 99 31 ) ) -RRB- cord-022774-wasdp2gh 99 32 . . . cord-022774-wasdp2gh 100 1 At at IN cord-022774-wasdp2gh 100 2 higher high JJR cord-022774-wasdp2gh 100 3 temperatures temperature NNS cord-022774-wasdp2gh 100 4 , , , cord-022774-wasdp2gh 100 5 e.g. e.g. RB cord-022774-wasdp2gh 100 6 37 37 CD cord-022774-wasdp2gh 100 7 ° ° NN cord-022774-wasdp2gh 100 8 C c NN cord-022774-wasdp2gh 100 9 , , , cord-022774-wasdp2gh 100 10 the the DT cord-022774-wasdp2gh 100 11 appearance appearance NN cord-022774-wasdp2gh 100 12 was be VBD cord-022774-wasdp2gh 100 13 similar similar JJ cord-022774-wasdp2gh 100 14 to to IN cord-022774-wasdp2gh 100 15 that that DT cord-022774-wasdp2gh 100 16 at at IN cord-022774-wasdp2gh 100 17 20 20 CD cord-022774-wasdp2gh 100 18 ° ° NN cord-022774-wasdp2gh 100 19 C c NN cord-022774-wasdp2gh 100 20 except except IN cord-022774-wasdp2gh 100 21 that that IN cord-022774-wasdp2gh 100 22 the the DT cord-022774-wasdp2gh 100 23 numbers number NNS cord-022774-wasdp2gh 100 24 of of IN cord-022774-wasdp2gh 100 25 transition transition NN cord-022774-wasdp2gh 100 26 vesicles vesicle NNS cord-022774-wasdp2gh 100 27 may may MD cord-022774-wasdp2gh 100 28 have have VB cord-022774-wasdp2gh 100 29 increased increase VBN cord-022774-wasdp2gh 100 30 slightly slightly RB cord-022774-wasdp2gh 100 31 . . . cord-022774-wasdp2gh 101 1 Cells cell NNS cord-022774-wasdp2gh 101 2 incubated incubate VBN cord-022774-wasdp2gh 101 3 at at IN cord-022774-wasdp2gh 101 4 temperatures temperature NNS cord-022774-wasdp2gh 101 5 below below IN cord-022774-wasdp2gh 101 6 16 16 CD cord-022774-wasdp2gh 101 7 ° ° NN cord-022774-wasdp2gh 101 8 C C NNP cord-022774-wasdp2gh 101 9 were be VBD cord-022774-wasdp2gh 101 10 similar similar JJ cord-022774-wasdp2gh 101 11 in in IN cord-022774-wasdp2gh 101 12 appearance appearance NN cord-022774-wasdp2gh 101 13 to to IN cord-022774-wasdp2gh 101 14 those those DT cord-022774-wasdp2gh 101 15 incubated incubate VBN cord-022774-wasdp2gh 101 16 at at IN cord-022774-wasdp2gh 101 17 16 16 CD cord-022774-wasdp2gh 101 18 ° ° NN cord-022774-wasdp2gh 101 19 C c NN cord-022774-wasdp2gh 101 20 except except IN cord-022774-wasdp2gh 101 21 for for IN cord-022774-wasdp2gh 101 22 small small JJ cord-022774-wasdp2gh 101 23 variations variation NNS cord-022774-wasdp2gh 101 24 in in IN cord-022774-wasdp2gh 101 25 numbers number NNS cord-022774-wasdp2gh 101 26 of of IN cord-022774-wasdp2gh 101 27 transition transition NN cord-022774-wasdp2gh 101 28 vesicles vesicle NNS cord-022774-wasdp2gh 101 29 ( ( -LRB- cord-022774-wasdp2gh 101 30 Fig Fig NNP cord-022774-wasdp2gh 101 31 . . . cord-022774-wasdp2gh 101 32 8) 8) NNP cord-022774-wasdp2gh 101 33 . . . cord-022774-wasdp2gh 102 1 Results result NNS cord-022774-wasdp2gh 102 2 with with IN cord-022774-wasdp2gh 102 3 30 30 CD cord-022774-wasdp2gh 102 4 rain rain NN cord-022774-wasdp2gh 102 5 of of IN cord-022774-wasdp2gh 102 6 incubation incubation NN cord-022774-wasdp2gh 102 7 ( ( -LRB- cord-022774-wasdp2gh 102 8 Fig Fig NNP cord-022774-wasdp2gh 102 9 . . NNP cord-022774-wasdp2gh 102 10 7 7 CD cord-022774-wasdp2gh 102 11 ) ) -RRB- cord-022774-wasdp2gh 102 12 and and CC cord-022774-wasdp2gh 102 13 60 60 CD cord-022774-wasdp2gh 102 14 min min NN cord-022774-wasdp2gh 102 15 of of IN cord-022774-wasdp2gh 102 16 incubation incubation NN cord-022774-wasdp2gh 102 17 ( ( -LRB- cord-022774-wasdp2gh 102 18 not not RB cord-022774-wasdp2gh 102 19 illustrated illustrate VBN cord-022774-wasdp2gh 102 20 ) ) -RRB- cord-022774-wasdp2gh 102 21 were be VBD cord-022774-wasdp2gh 102 22 comparable comparable JJ cord-022774-wasdp2gh 102 23 . . . cord-022774-wasdp2gh 103 1 Quantitation quantitation NN cord-022774-wasdp2gh 103 2 of of IN cord-022774-wasdp2gh 103 3 vesicle vesicle NN cord-022774-wasdp2gh 103 4 numbers number NNS cord-022774-wasdp2gh 103 5 of of IN cord-022774-wasdp2gh 103 6 transition transition NN cord-022774-wasdp2gh 103 7 vesicles vesicle NNS cord-022774-wasdp2gh 103 8 associated associate VBN cord-022774-wasdp2gh 103 9 with with IN cord-022774-wasdp2gh 103 10 Golgi Golgi NNP cord-022774-wasdp2gh 103 11 apparatus apparatus NN cord-022774-wasdp2gh 103 12 stacks stack NNS cord-022774-wasdp2gh 103 13 of of IN cord-022774-wasdp2gh 103 14 BHK BHK NNP cord-022774-wasdp2gh 103 15 cells cell NNS cord-022774-wasdp2gh 103 16 gave give VBD cord-022774-wasdp2gh 103 17 results result NNS cord-022774-wasdp2gh 103 18 ( ( -LRB- cord-022774-wasdp2gh 103 19 Fig Fig NNP cord-022774-wasdp2gh 103 20 . . . cord-022774-wasdp2gh 103 21 8 8 CD cord-022774-wasdp2gh 103 22 ) ) -RRB- cord-022774-wasdp2gh 103 23 similar similar JJ cord-022774-wasdp2gh 103 24 to to IN cord-022774-wasdp2gh 103 25 those those DT cord-022774-wasdp2gh 103 26 observed observe VBN cord-022774-wasdp2gh 103 27 for for IN cord-022774-wasdp2gh 103 28 liver liver NN cord-022774-wasdp2gh 103 29 slices slice NNS cord-022774-wasdp2gh 104 1 ( ( -LRB- cord-022774-wasdp2gh 104 2 Fig Fig NNP cord-022774-wasdp2gh 104 3 . . NNP cord-022774-wasdp2gh 104 4 5 5 CD cord-022774-wasdp2gh 104 5 ) ) -RRB- cord-022774-wasdp2gh 104 6 . . . cord-022774-wasdp2gh 105 1 The the DT cord-022774-wasdp2gh 105 2 r r NN cord-022774-wasdp2gh 105 3 , , , cord-022774-wasdp2gh 105 4 lost lose VBN cord-022774-wasdp2gh 105 5 characteristic characteristic JJ cord-022774-wasdp2gh 105 6 feature feature NN cord-022774-wasdp2gh 105 7 of of IN cord-022774-wasdp2gh 105 8 these these DT cord-022774-wasdp2gh 105 9 data datum NNS cord-022774-wasdp2gh 105 10 was be VBD cord-022774-wasdp2gh 105 11 the the DT cord-022774-wasdp2gh 105 12 precipitous precipitous JJ cord-022774-wasdp2gh 105 13 drop drop NN cord-022774-wasdp2gh 105 14 in in IN cord-022774-wasdp2gh 105 15 number number NN cord-022774-wasdp2gh 105 16 of of IN cord-022774-wasdp2gh 105 17 transition transition NN cord-022774-wasdp2gh 105 18 vesicles vesicle NNS cord-022774-wasdp2gh 105 19 between between IN cord-022774-wasdp2gh 105 20 18 18 CD cord-022774-wasdp2gh 105 21 ° ° NN cord-022774-wasdp2gh 105 22 C c NN cord-022774-wasdp2gh 105 23 and and CC cord-022774-wasdp2gh 105 24 20 20 CD cord-022774-wasdp2gh 105 25 ° ° NN cord-022774-wasdp2gh 105 26 C c NN cord-022774-wasdp2gh 105 27 . . . cord-022774-wasdp2gh 106 1 Thereafter thereafter RB cord-022774-wasdp2gh 106 2 , , , cord-022774-wasdp2gh 106 3 with with IN cord-022774-wasdp2gh 106 4 increasing increase VBG cord-022774-wasdp2gh 106 5 temperature temperature NN cord-022774-wasdp2gh 106 6 , , , cord-022774-wasdp2gh 106 7 the the DT cord-022774-wasdp2gh 106 8 number number NN cord-022774-wasdp2gh 106 9 of of IN cord-022774-wasdp2gh 106 10 vesicles vesicle NNS cord-022774-wasdp2gh 106 11 present present JJ cord-022774-wasdp2gh 106 12 increased increase VBD cord-022774-wasdp2gh 106 13 slightly slightly RB cord-022774-wasdp2gh 106 14 . . . cord-022774-wasdp2gh 107 1 As as IN cord-022774-wasdp2gh 107 2 with with IN cord-022774-wasdp2gh 107 3 liver liver NN cord-022774-wasdp2gh 107 4 cells cell NNS cord-022774-wasdp2gh 107 5 , , , cord-022774-wasdp2gh 107 6 the the DT cord-022774-wasdp2gh 107 7 average average JJ cord-022774-wasdp2gh 107 8 diameter diameter NN cord-022774-wasdp2gh 107 9 of of IN cord-022774-wasdp2gh 107 10 the the DT cord-022774-wasdp2gh 107 11 transition transition NN cord-022774-wasdp2gh 107 12 vesicles vesicle NNS cord-022774-wasdp2gh 107 13 in in IN cord-022774-wasdp2gh 107 14 BHK BHK NNP cord-022774-wasdp2gh 107 15 cells cell NNS cord-022774-wasdp2gh 107 16 at at IN cord-022774-wasdp2gh 107 17 4 4 CD cord-022774-wasdp2gh 107 18 ° ° NN cord-022774-wasdp2gh 107 19 C C NNP cord-022774-wasdp2gh 107 20 was be VBD cord-022774-wasdp2gh 107 21 55 55 CD cord-022774-wasdp2gh 107 22 .+ .+ CD cord-022774-wasdp2gh 107 23 4 4 CD cord-022774-wasdp2gh 107 24 / / SYM cord-022774-wasdp2gh 107 25 zm zm NN cord-022774-wasdp2gh 107 26 but but CC cord-022774-wasdp2gh 107 27 increased increase VBD cord-022774-wasdp2gh 107 28 to to IN cord-022774-wasdp2gh 107 29 66 66 CD cord-022774-wasdp2gh 107 30 + + SYM cord-022774-wasdp2gh 107 31 5 5 CD cord-022774-wasdp2gh 108 1 ~m ~m JJ cord-022774-wasdp2gh 109 1 at at IN cord-022774-wasdp2gh 109 2 370C. 370c. CD cord-022774-wasdp2gh 109 3 . . . cord-022774-wasdp2gh 110 1 The the DT cord-022774-wasdp2gh 110 2 appearance appearance NN cord-022774-wasdp2gh 110 3 at at IN cord-022774-wasdp2gh 110 4 37 37 CD cord-022774-wasdp2gh 110 5 ° ° NN cord-022774-wasdp2gh 110 6 C C NNP cord-022774-wasdp2gh 110 7 is be VBZ cord-022774-wasdp2gh 110 8 similar similar JJ cord-022774-wasdp2gh 110 9 to to IN cord-022774-wasdp2gh 110 10 that that DT cord-022774-wasdp2gh 110 11 at at IN cord-022774-wasdp2gh 110 12 20 20 CD cord-022774-wasdp2gh 110 13 ° ° NN cord-022774-wasdp2gh 110 14 C c NN cord-022774-wasdp2gh 110 15 except except IN cord-022774-wasdp2gh 110 16 that that DT cord-022774-wasdp2gh 110 17 transition transition NN cord-022774-wasdp2gh 110 18 vesicles vesicle NNS cord-022774-wasdp2gh 110 19 appear appear VBP cord-022774-wasdp2gh 110 20 to to TO cord-022774-wasdp2gh 110 21 be be VB cord-022774-wasdp2gh 110 22 more more RBR cord-022774-wasdp2gh 110 23 numerous numerous JJ cord-022774-wasdp2gh 110 24 . . . cord-022774-wasdp2gh 111 1 Bar=0.5/~m Bar=0.5/~m NNP cord-022774-wasdp2gh 111 2 . . . cord-022774-wasdp2gh 112 1 brane brane NN cord-022774-wasdp2gh 112 2 from from IN cord-022774-wasdp2gh 112 3 transitional transitional JJ cord-022774-wasdp2gh 112 4 endoplasmic endoplasmic NN cord-022774-wasdp2gh 112 5 reticulum reticulum NN cord-022774-wasdp2gh 112 6 donor donor NN cord-022774-wasdp2gh 112 7 that that WDT cord-022774-wasdp2gh 112 8 was be VBD cord-022774-wasdp2gh 112 9 prepared prepare VBN cord-022774-wasdp2gh 112 10 from from IN cord-022774-wasdp2gh 112 11 liver liver NN cord-022774-wasdp2gh 112 12 slices slice NNS cord-022774-wasdp2gh 112 13 labeled label VBN cord-022774-wasdp2gh 112 14 with with IN cord-022774-wasdp2gh 112 15 [ [ -LRB- cord-022774-wasdp2gh 112 16 14C]leucine 14c]leucine CD cord-022774-wasdp2gh 112 17 to to IN cord-022774-wasdp2gh 112 18 unlabeled unlabeled JJ cord-022774-wasdp2gh 112 19 Golgi Golgi NNP cord-022774-wasdp2gh 112 20 apparatus apparatus NN cord-022774-wasdp2gh 112 21 acceptor acceptor NN cord-022774-wasdp2gh 112 22 . . . cord-022774-wasdp2gh 113 1 Transfer transfer NN cord-022774-wasdp2gh 113 2 was be VBD cord-022774-wasdp2gh 113 3 8-fold 8-fold CD cord-022774-wasdp2gh 113 4 greater great JJR cord-022774-wasdp2gh 113 5 at at IN cord-022774-wasdp2gh 113 6 37 37 CD cord-022774-wasdp2gh 113 7 ° ° NN cord-022774-wasdp2gh 113 8 C c NN cord-022774-wasdp2gh 113 9 than than IN cord-022774-wasdp2gh 113 10 at at IN cord-022774-wasdp2gh 113 11 4 4 CD cord-022774-wasdp2gh 113 12 ° ° NN cord-022774-wasdp2gh 113 13 C c NN cord-022774-wasdp2gh 113 14 commensurate commensurate NN cord-022774-wasdp2gh 113 15 with with IN cord-022774-wasdp2gh 113 16 a a DT cord-022774-wasdp2gh 113 17 Ql0 ql0 NN cord-022774-wasdp2gh 113 18 of of IN cord-022774-wasdp2gh 113 19 2 2 CD cord-022774-wasdp2gh 113 20 for for IN cord-022774-wasdp2gh 113 21 the the DT cord-022774-wasdp2gh 113 22 overall overall JJ cord-022774-wasdp2gh 113 23 transfer transfer NN cord-022774-wasdp2gh 113 24 process process NN cord-022774-wasdp2gh 113 25 . . . cord-022774-wasdp2gh 114 1 Little little JJ cord-022774-wasdp2gh 114 2 or or CC cord-022774-wasdp2gh 114 3 no no DT cord-022774-wasdp2gh 114 4 transfer transfer NN cord-022774-wasdp2gh 114 5 was be VBD cord-022774-wasdp2gh 114 6 observed observe VBN cord-022774-wasdp2gh 114 7 at at IN cord-022774-wasdp2gh 114 8 4 4 CD cord-022774-wasdp2gh 114 9 ° ° NN cord-022774-wasdp2gh 114 10 C c NN cord-022774-wasdp2gh 114 11 compared compare VBN cord-022774-wasdp2gh 114 12 to to IN cord-022774-wasdp2gh 114 13 37 37 CD cord-022774-wasdp2gh 114 14 ° ° NN cord-022774-wasdp2gh 114 15 C c NN cord-022774-wasdp2gh 114 16 even even RB cord-022774-wasdp2gh 114 17 in in IN cord-022774-wasdp2gh 114 18 the the DT cord-022774-wasdp2gh 114 19 presence presence NN cord-022774-wasdp2gh 114 20 of of IN cord-022774-wasdp2gh 114 21 the the DT cord-022774-wasdp2gh 114 22 complete complete JJ cord-022774-wasdp2gh 114 23 incubation incubation NN cord-022774-wasdp2gh 114 24 system system NN cord-022774-wasdp2gh 114 25 containing contain VBG cord-022774-wasdp2gh 114 26 ATP ATP NNP cord-022774-wasdp2gh 114 27 , , , cord-022774-wasdp2gh 114 28 ATP ATP NNP cord-022774-wasdp2gh 114 29 regenerating regenerate VBG cord-022774-wasdp2gh 114 30 system system NN cord-022774-wasdp2gh 114 31 and and CC cord-022774-wasdp2gh 114 32 cytosol cytosol NN cord-022774-wasdp2gh 114 33 . . . cord-022774-wasdp2gh 115 1 In in IN cord-022774-wasdp2gh 115 2 the the DT cord-022774-wasdp2gh 115 3 present present JJ cord-022774-wasdp2gh 115 4 study study NN cord-022774-wasdp2gh 115 5 , , , cord-022774-wasdp2gh 115 6 the the DT cord-022774-wasdp2gh 115 7 transition transition NN cord-022774-wasdp2gh 115 8 element element NN cord-022774-wasdp2gh 115 9 fractions fraction NNS cord-022774-wasdp2gh 115 10 from from IN cord-022774-wasdp2gh 115 11 rat rat NN cord-022774-wasdp2gh 115 12 liver liver NN cord-022774-wasdp2gh 115 13 exhibit exhibit NN cord-022774-wasdp2gh 115 14 temperature temperature NN cord-022774-wasdp2gh 115 15 dependent dependent JJ cord-022774-wasdp2gh 115 16 formation formation NN cord-022774-wasdp2gh 115 17 of of IN cord-022774-wasdp2gh 115 18 vesicles vesicle NNS cord-022774-wasdp2gh 115 19 similar similar JJ cord-022774-wasdp2gh 115 20 to to IN cord-022774-wasdp2gh 115 21 that that DT cord-022774-wasdp2gh 115 22 observed observe VBN cord-022774-wasdp2gh 115 23 in in IN cord-022774-wasdp2gh 115 24 situ situ NN cord-022774-wasdp2gh 115 25 . . . cord-022774-wasdp2gh 116 1 The the DT cord-022774-wasdp2gh 116 2 formation formation NN cord-022774-wasdp2gh 116 3 of of IN cord-022774-wasdp2gh 116 4 = = SYM cord-022774-wasdp2gh 116 5 50 50 CD cord-022774-wasdp2gh 116 6 - - HYPH cord-022774-wasdp2gh 116 7 70/~m 70/~m NN cord-022774-wasdp2gh 116 8 transition transition NN cord-022774-wasdp2gh 116 9 vesicle vesicle NN cord-022774-wasdp2gh 116 10 - - HYPH cord-022774-wasdp2gh 116 11 like like JJ cord-022774-wasdp2gh 116 12 structures structure NNS cord-022774-wasdp2gh 116 13 over over RB cord-022774-wasdp2gh 116 14 and and CC cord-022774-wasdp2gh 116 15 above above IN cord-022774-wasdp2gh 116 16 those those DT cord-022774-wasdp2gh 116 17 initially initially RB cord-022774-wasdp2gh 116 18 present present JJ cord-022774-wasdp2gh 116 19 began begin VBD cord-022774-wasdp2gh 116 20 at at IN cord-022774-wasdp2gh 116 21 temperatures temperature NNS cord-022774-wasdp2gh 116 22 of of IN cord-022774-wasdp2gh 116 23 18 18 CD cord-022774-wasdp2gh 116 24 ° ° NN cord-022774-wasdp2gh 116 25 C c NN cord-022774-wasdp2gh 116 26 and and CC cord-022774-wasdp2gh 116 27 above above RB cord-022774-wasdp2gh 116 28 . . . cord-022774-wasdp2gh 117 1 The the DT cord-022774-wasdp2gh 117 2 origin origin NN cord-022774-wasdp2gh 117 3 of of IN cord-022774-wasdp2gh 117 4 at at RB cord-022774-wasdp2gh 117 5 least least JJS cord-022774-wasdp2gh 117 6 a a DT cord-022774-wasdp2gh 117 7 major major JJ cord-022774-wasdp2gh 117 8 fraction fraction NN cord-022774-wasdp2gh 117 9 of of IN cord-022774-wasdp2gh 117 10 these these DT cord-022774-wasdp2gh 117 11 vesicles vesicle NNS cord-022774-wasdp2gh 117 12 was be VBD cord-022774-wasdp2gh 117 13 from from IN cord-022774-wasdp2gh 117 14 the the DT cord-022774-wasdp2gh 117 15 transition transition NN cord-022774-wasdp2gh 117 16 endoplasmic endoplasmic NN cord-022774-wasdp2gh 117 17 reticulum reticulum NN cord-022774-wasdp2gh 117 18 vesicles vesicle NNS cord-022774-wasdp2gh 117 19 present present JJ cord-022774-wasdp2gh 117 20 in in IN cord-022774-wasdp2gh 117 21 the the DT cord-022774-wasdp2gh 117 22 fraction fraction NN cord-022774-wasdp2gh 117 23 . . . cord-022774-wasdp2gh 118 1 Many many JJ cord-022774-wasdp2gh 118 2 of of IN cord-022774-wasdp2gh 118 3 the the DT cord-022774-wasdp2gh 118 4 vesicles vesicle NNS cord-022774-wasdp2gh 118 5 re- re- RB cord-022774-wasdp2gh 118 6 mained maine VBD cord-022774-wasdp2gh 118 7 attached attach VBN cord-022774-wasdp2gh 118 8 to to IN cord-022774-wasdp2gh 118 9 larger large JJR cord-022774-wasdp2gh 118 10 endoplasmic endoplasmic NN cord-022774-wasdp2gh 118 11 reticulum reticulum NN cord-022774-wasdp2gh 118 12 vesicles vesicle NNS cord-022774-wasdp2gh 118 13 by by IN cord-022774-wasdp2gh 118 14 a a DT cord-022774-wasdp2gh 118 15 narrow narrow JJ cord-022774-wasdp2gh 118 16 stalk stalk NN cord-022774-wasdp2gh 118 17 ( ( -LRB- cord-022774-wasdp2gh 118 18 Fig fig NN cord-022774-wasdp2gh 118 19 . . . cord-022774-wasdp2gh 118 20 1 1 CD cord-022774-wasdp2gh 118 21 ) ) -RRB- cord-022774-wasdp2gh 118 22 . . . cord-022774-wasdp2gh 119 1 The the DT cord-022774-wasdp2gh 119 2 attachment attachment NN cord-022774-wasdp2gh 119 3 of of IN cord-022774-wasdp2gh 119 4 the the DT cord-022774-wasdp2gh 119 5 vesicles vesicle NNS cord-022774-wasdp2gh 119 6 during during IN cord-022774-wasdp2gh 119 7 tissue tissue NN cord-022774-wasdp2gh 119 8 homogenization homogenization NN cord-022774-wasdp2gh 119 9 is be VBZ cord-022774-wasdp2gh 119 10 ruled rule VBN cord-022774-wasdp2gh 119 11 out out RP cord-022774-wasdp2gh 119 12 by by IN cord-022774-wasdp2gh 119 13 the the DT cord-022774-wasdp2gh 119 14 absolute absolute JJ cord-022774-wasdp2gh 119 15 dependency dependency NN cord-022774-wasdp2gh 119 16 of of IN cord-022774-wasdp2gh 119 17 their -PRON- PRP$ cord-022774-wasdp2gh 119 18 presence presence NN cord-022774-wasdp2gh 119 19 upon upon IN cord-022774-wasdp2gh 119 20 ATP ATP NNP cord-022774-wasdp2gh 119 21 [ [ -LRB- cord-022774-wasdp2gh 119 22 I i NN cord-022774-wasdp2gh 119 23 3 3 CD cord-022774-wasdp2gh 119 24 ] ] -RRB- cord-022774-wasdp2gh 119 25 . . . cord-022774-wasdp2gh 120 1 Specificity specificity NN cord-022774-wasdp2gh 120 2 is be VBZ cord-022774-wasdp2gh 120 3 also also RB cord-022774-wasdp2gh 120 4 shown show VBN cord-022774-wasdp2gh 120 5 by by IN cord-022774-wasdp2gh 120 6 the the DT cord-022774-wasdp2gh 120 7 absence absence NN cord-022774-wasdp2gh 120 8 of of IN cord-022774-wasdp2gh 120 9 such such JJ cord-022774-wasdp2gh 120 10 vesicles vesicle NNS cord-022774-wasdp2gh 120 11 from from IN cord-022774-wasdp2gh 120 12 incubated incubate VBN cord-022774-wasdp2gh 120 13 preparations preparation NNS cord-022774-wasdp2gh 120 14 consisting consist VBG cord-022774-wasdp2gh 120 15 only only RB cord-022774-wasdp2gh 120 16 of of IN cord-022774-wasdp2gh 120 17 conventional conventional JJ cord-022774-wasdp2gh 120 18 rough rough JJ cord-022774-wasdp2gh 120 19 endoplasmic endoplasmic NN cord-022774-wasdp2gh 120 20 reticulum reticulum NN cord-022774-wasdp2gh 120 21 collected collect VBN cord-022774-wasdp2gh 120 22 from from IN cord-022774-wasdp2gh 120 23 gradient gradient NN cord-022774-wasdp2gh 120 24 fractions fraction NNS cord-022774-wasdp2gh 120 25 of of IN cord-022774-wasdp2gh 120 26 density density NN cord-022774-wasdp2gh 120 27 greater great JJR cord-022774-wasdp2gh 120 28 than than IN cord-022774-wasdp2gh 120 29 those those DT cord-022774-wasdp2gh 120 30 yielding yield VBG cord-022774-wasdp2gh 120 31 the the DT cord-022774-wasdp2gh 120 32 responsive responsive JJ cord-022774-wasdp2gh 120 33 transitional transitional JJ cord-022774-wasdp2gh 120 34 membrane membrane NN cord-022774-wasdp2gh 120 35 elements element NNS cord-022774-wasdp2gh 120 36 [ [ -LRB- cord-022774-wasdp2gh 120 37 15 15 CD cord-022774-wasdp2gh 120 38 ] ] -RRB- cord-022774-wasdp2gh 120 39 . . . cord-022774-wasdp2gh 121 1 In in IN cord-022774-wasdp2gh 121 2 situ situ NN cord-022774-wasdp2gh 121 3 , , , cord-022774-wasdp2gh 121 4 transitional transitional JJ cord-022774-wasdp2gh 121 5 endoplasmic endoplasmic NN cord-022774-wasdp2gh 121 6 reticulum reticulum NN cord-022774-wasdp2gh 121 7 of of IN cord-022774-wasdp2gh 121 8 liver liver NN cord-022774-wasdp2gh 121 9 slices slice NNS cord-022774-wasdp2gh 121 10 incubated incubate VBN cord-022774-wasdp2gh 121 11 at at IN cord-022774-wasdp2gh 121 12 different different JJ cord-022774-wasdp2gh 121 13 temperature temperature NN cord-022774-wasdp2gh 121 14 conditions condition NNS cord-022774-wasdp2gh 121 15 behaved behave VBD cord-022774-wasdp2gh 121 16 similarly similarly RB cord-022774-wasdp2gh 121 17 . . . cord-022774-wasdp2gh 122 1 Over over IN cord-022774-wasdp2gh 122 2 the the DT cord-022774-wasdp2gh 122 3 range range NN cord-022774-wasdp2gh 122 4 4 4 CD cord-022774-wasdp2gh 122 5 ° ° NN cord-022774-wasdp2gh 122 6 C c NN cord-022774-wasdp2gh 122 7 to to IN cord-022774-wasdp2gh 122 8 12 12 CD cord-022774-wasdp2gh 122 9 ° ° NN cord-022774-wasdp2gh 122 10 C c NN cord-022774-wasdp2gh 122 11 , , , cord-022774-wasdp2gh 122 12 there there EX cord-022774-wasdp2gh 122 13 was be VBD cord-022774-wasdp2gh 122 14 little little JJ cord-022774-wasdp2gh 122 15 evidence evidence NN cord-022774-wasdp2gh 122 16 of of IN cord-022774-wasdp2gh 122 17 transition transition NN cord-022774-wasdp2gh 122 18 vesicle vesicle NN cord-022774-wasdp2gh 122 19 formation formation NN cord-022774-wasdp2gh 122 20 . . . cord-022774-wasdp2gh 123 1 However however RB cord-022774-wasdp2gh 123 2 , , , cord-022774-wasdp2gh 123 3 between between IN cord-022774-wasdp2gh 123 4 16 16 CD cord-022774-wasdp2gh 123 5 ° ° NN cord-022774-wasdp2gh 123 6 C c NN cord-022774-wasdp2gh 123 7 and and CC cord-022774-wasdp2gh 123 8 18 18 CD cord-022774-wasdp2gh 123 9 ° ° NN cord-022774-wasdp2gh 123 10 C c NN cord-022774-wasdp2gh 123 11 not not RB cord-022774-wasdp2gh 123 12 only only RB cord-022774-wasdp2gh 123 13 were be VBD cord-022774-wasdp2gh 123 14 transition transition NN cord-022774-wasdp2gh 123 15 vesicles vesicle NNS cord-022774-wasdp2gh 123 16 formed form VBN cord-022774-wasdp2gh 123 17 but but CC cord-022774-wasdp2gh 123 18 they -PRON- PRP cord-022774-wasdp2gh 123 19 accumulated accumulate VBD cord-022774-wasdp2gh 123 20 as as IN cord-022774-wasdp2gh 123 21 shown show VBN cord-022774-wasdp2gh 123 22 by by IN cord-022774-wasdp2gh 123 23 others other NNS cord-022774-wasdp2gh 123 24 , , , cord-022774-wasdp2gh 123 25 presumably presumably RB cord-022774-wasdp2gh 123 26 due due IN cord-022774-wasdp2gh 123 27 to to IN cord-022774-wasdp2gh 123 28 an an DT cord-022774-wasdp2gh 123 29 inability inability NN cord-022774-wasdp2gh 123 30 of of IN cord-022774-wasdp2gh 123 31 the the DT cord-022774-wasdp2gh 123 32 vesicles vesicle NNS cord-022774-wasdp2gh 123 33 to to TO cord-022774-wasdp2gh 123 34 fuse fuse VB cord-022774-wasdp2gh 123 35 with with IN cord-022774-wasdp2gh 123 36 the the DT cord-022774-wasdp2gh 123 37 cis cis NNP cord-022774-wasdp2gh 123 38 Golgi Golgi NNP cord-022774-wasdp2gh 123 39 apparatus apparatus NN cord-022774-wasdp2gh 123 40 at at IN cord-022774-wasdp2gh 123 41 temperatures temperature NNS cord-022774-wasdp2gh 123 42 of of IN cord-022774-wasdp2gh 123 43 18 18 CD cord-022774-wasdp2gh 123 44 ° ° NN cord-022774-wasdp2gh 123 45 C c NN cord-022774-wasdp2gh 123 46 or or CC cord-022774-wasdp2gh 123 47 below below IN cord-022774-wasdp2gh 123 48 [ [ -LRB- cord-022774-wasdp2gh 123 49 3 3 CD cord-022774-wasdp2gh 123 50 , , , cord-022774-wasdp2gh 123 51 5 5 CD cord-022774-wasdp2gh 123 52 , , , cord-022774-wasdp2gh 123 53 10 10 CD cord-022774-wasdp2gh 123 54 , , , cord-022774-wasdp2gh 123 55 18 18 CD cord-022774-wasdp2gh 123 56 , , , cord-022774-wasdp2gh 123 57 19 19 CD cord-022774-wasdp2gh 123 58 ] ] -RRB- cord-022774-wasdp2gh 123 59 . . . cord-022774-wasdp2gh 124 1 Quite quite RB cord-022774-wasdp2gh 124 2 abruptly abruptly RB cord-022774-wasdp2gh 124 3 , , , cord-022774-wasdp2gh 124 4 betwcen betwcen NNP cord-022774-wasdp2gh 124 5 18 18 CD cord-022774-wasdp2gh 124 6 ° ° NN cord-022774-wasdp2gh 124 7 C c NN cord-022774-wasdp2gh 124 8 and and CC cord-022774-wasdp2gh 124 9 20 20 CD cord-022774-wasdp2gh 124 10 ° ° NN cord-022774-wasdp2gh 124 11 C c NN cord-022774-wasdp2gh 124 12 , , , cord-022774-wasdp2gh 124 13 the the DT cord-022774-wasdp2gh 124 14 number number NN cord-022774-wasdp2gh 124 15 of of IN cord-022774-wasdp2gh 124 16 transition transition NN cord-022774-wasdp2gh 124 17 vesicles vesicle NNS cord-022774-wasdp2gh 124 18 declined decline VBD cord-022774-wasdp2gh 124 19 as as IN cord-022774-wasdp2gh 124 20 the the DT cord-022774-wasdp2gh 124 21 temperature temperature NN cord-022774-wasdp2gh 124 22 block block NN cord-022774-wasdp2gh 124 23 that that WDT cord-022774-wasdp2gh 124 24 prevented prevent VBD cord-022774-wasdp2gh 124 25 vesicle vesicle NN cord-022774-wasdp2gh 124 26 fusion fusion NN cord-022774-wasdp2gh 124 27 with with IN cord-022774-wasdp2gh 124 28 the the DT cord-022774-wasdp2gh 124 29 Golgi Golgi NNP cord-022774-wasdp2gh 124 30 apparatus apparatus NN cord-022774-wasdp2gh 124 31 was be VBD cord-022774-wasdp2gh 124 32 relieved relieve VBN cord-022774-wasdp2gh 124 33 [ [ -LRB- cord-022774-wasdp2gh 124 34 3 3 CD cord-022774-wasdp2gh 124 35 , , , cord-022774-wasdp2gh 124 36 5 5 CD cord-022774-wasdp2gh 124 37 , , , cord-022774-wasdp2gh 124 38 18 18 CD cord-022774-wasdp2gh 124 39 , , , cord-022774-wasdp2gh 124 40 19 19 CD cord-022774-wasdp2gh 124 41 ] ] -RRB- cord-022774-wasdp2gh 124 42 . . . cord-022774-wasdp2gh 125 1 Interestingly interestingly RB cord-022774-wasdp2gh 125 2 , , , cord-022774-wasdp2gh 125 3 not not RB cord-022774-wasdp2gh 125 4 only only RB cord-022774-wasdp2gh 125 5 was be VBD cord-022774-wasdp2gh 125 6 there there EX cord-022774-wasdp2gh 125 7 an an DT cord-022774-wasdp2gh 125 8 effect effect NN cord-022774-wasdp2gh 125 9 of of IN cord-022774-wasdp2gh 125 10 temperature temperature NN cord-022774-wasdp2gh 125 11 on on IN cord-022774-wasdp2gh 125 12 transition transition NN cord-022774-wasdp2gh 125 13 vesicle vesicle NN cord-022774-wasdp2gh 125 14 numbers number NNS cord-022774-wasdp2gh 125 15 but but CC cord-022774-wasdp2gh 125 16 on on IN cord-022774-wasdp2gh 125 17 the the DT cord-022774-wasdp2gh 125 18 distribution distribution NN cord-022774-wasdp2gh 125 19 of of IN cord-022774-wasdp2gh 125 20 diameters diameter NNS cord-022774-wasdp2gh 125 21 as as RB cord-022774-wasdp2gh 125 22 well well RB cord-022774-wasdp2gh 125 23 . . . cord-022774-wasdp2gh 126 1 At at IN cord-022774-wasdp2gh 126 2 37 37 CD cord-022774-wasdp2gh 126 3 ° ° NN cord-022774-wasdp2gh 126 4 C c NN cord-022774-wasdp2gh 126 5 in in IN cord-022774-wasdp2gh 126 6 situ situ NN cord-022774-wasdp2gh 126 7 , , , cord-022774-wasdp2gh 126 8 the the DT cord-022774-wasdp2gh 126 9 distribution distribution NN cord-022774-wasdp2gh 126 10 of of IN cord-022774-wasdp2gh 126 11 transition transition NN cord-022774-wasdp2gh 126 12 vesicle vesicle NN cord-022774-wasdp2gh 126 13 diameters diameter NNS cord-022774-wasdp2gh 126 14 was be VBD cord-022774-wasdp2gh 126 15 shifted shift VBN cord-022774-wasdp2gh 126 16 in in IN cord-022774-wasdp2gh 126 17 the the DT cord-022774-wasdp2gh 126 18 direction direction NN cord-022774-wasdp2gh 126 19 of of IN cord-022774-wasdp2gh 126 20 larger large JJR cord-022774-wasdp2gh 126 21 diameters diameter NNS cord-022774-wasdp2gh 126 22 such such JJ cord-022774-wasdp2gh 126 23 that that IN cord-022774-wasdp2gh 126 24 their -PRON- PRP$ cord-022774-wasdp2gh 126 25 overall overall JJ cord-022774-wasdp2gh 126 26 average average JJ cord-022774-wasdp2gh 126 27 diameter diameter NN cord-022774-wasdp2gh 126 28 was be VBD cord-022774-wasdp2gh 126 29 increased increase VBN cord-022774-wasdp2gh 126 30 from from IN cord-022774-wasdp2gh 126 31 55 55 CD cord-022774-wasdp2gh 126 32 to to IN cord-022774-wasdp2gh 126 33 65/~m 65/~m NNP cord-022774-wasdp2gh 126 34 . . . cord-022774-wasdp2gh 127 1 For for IN cord-022774-wasdp2gh 127 2 these these DT cord-022774-wasdp2gh 127 3 measurements measurement NNS cord-022774-wasdp2gh 127 4 , , , cord-022774-wasdp2gh 127 5 the the DT cord-022774-wasdp2gh 127 6 transition transition NN cord-022774-wasdp2gh 127 7 vesicles vesicle NNS cord-022774-wasdp2gh 127 8 were be VBD cord-022774-wasdp2gh 127 9 identified identify VBN cord-022774-wasdp2gh 127 10 not not RB cord-022774-wasdp2gh 127 11 only only RB cord-022774-wasdp2gh 127 12 from from IN cord-022774-wasdp2gh 127 13 their -PRON- PRP$ cord-022774-wasdp2gh 127 14 characteristic characteristic JJ cord-022774-wasdp2gh 127 15 appearance appearance NN cord-022774-wasdp2gh 127 16 but but CC cord-022774-wasdp2gh 127 17 from from IN cord-022774-wasdp2gh 127 18 their -PRON- PRP$ cord-022774-wasdp2gh 127 19 spatial spatial JJ cord-022774-wasdp2gh 127 20 location location NN cord-022774-wasdp2gh 127 21 within within IN cord-022774-wasdp2gh 127 22 the the DT cord-022774-wasdp2gh 127 23 cytoplasm cytoplasm NN cord-022774-wasdp2gh 127 24 . . . cord-022774-wasdp2gh 128 1 In in IN cord-022774-wasdp2gh 128 2 situ situ JJ cord-022774-wasdp2gh 128 3 measurements measurement NNS cord-022774-wasdp2gh 128 4 from from IN cord-022774-wasdp2gh 128 5 structures structure NNS cord-022774-wasdp2gh 128 6 other other JJ cord-022774-wasdp2gh 128 7 than than IN cord-022774-wasdp2gh 128 8 transition transition NN cord-022774-wasdp2gh 128 9 vesicles vesicle NNS cord-022774-wasdp2gh 128 10 would would MD cord-022774-wasdp2gh 128 11 have have VB cord-022774-wasdp2gh 128 12 been be VBN cord-022774-wasdp2gh 128 13 largely largely RB cord-022774-wasdp2gh 128 14 eliminated eliminate VBN cord-022774-wasdp2gh 128 15 . . . cord-022774-wasdp2gh 129 1 When when WRB cord-022774-wasdp2gh 129 2 a a DT cord-022774-wasdp2gh 129 3 FIGURE figure NN cord-022774-wasdp2gh 129 4 9 9 CD cord-022774-wasdp2gh 129 5 . . . cord-022774-wasdp2gh 130 1 --Effect --Effect : cord-022774-wasdp2gh 130 2 of of IN cord-022774-wasdp2gh 130 3 CoCI CoCI NNP cord-022774-wasdp2gh 130 4 2 2 CD cord-022774-wasdp2gh 130 5 and and CC cord-022774-wasdp2gh 130 6 KCN KCN NNP cord-022774-wasdp2gh 130 7 on on IN cord-022774-wasdp2gh 130 8 transitional transitional JJ cord-022774-wasdp2gh 130 9 endoplasmic endoplasmic NN cord-022774-wasdp2gh 130 10 reticulum reticulum NN cord-022774-wasdp2gh 130 11 of of IN cord-022774-wasdp2gh 130 12 BHK BHK NNP cord-022774-wasdp2gh 130 13 cells cell NNS cord-022774-wasdp2gh 130 14 . . . cord-022774-wasdp2gh 131 1 A a LS cord-022774-wasdp2gh 131 2 , , , cord-022774-wasdp2gh 131 3 Control Control NNP cord-022774-wasdp2gh 131 4 cells cell NNS cord-022774-wasdp2gh 131 5 incubated incubate VBN cord-022774-wasdp2gh 131 6 in in IN cord-022774-wasdp2gh 131 7 the the DT cord-022774-wasdp2gh 131 8 absence absence NN cord-022774-wasdp2gh 131 9 of of IN cord-022774-wasdp2gh 131 10 inhibitor inhibitor NN cord-022774-wasdp2gh 131 11 . . . cord-022774-wasdp2gh 132 1 B b NN cord-022774-wasdp2gh 132 2 , , , cord-022774-wasdp2gh 132 3 Incubation Incubation NNP cord-022774-wasdp2gh 132 4 for for IN cord-022774-wasdp2gh 132 5 30 30 CD cord-022774-wasdp2gh 132 6 rain rain NN cord-022774-wasdp2gh 132 7 in in IN cord-022774-wasdp2gh 132 8 the the DT cord-022774-wasdp2gh 132 9 presence presence NN cord-022774-wasdp2gh 132 10 of of IN cord-022774-wasdp2gh 132 11 KCN KCN NNP cord-022774-wasdp2gh 132 12 . . . cord-022774-wasdp2gh 133 1 The the DT cord-022774-wasdp2gh 133 2 appearance appearance NN cord-022774-wasdp2gh 133 3 is be VBZ cord-022774-wasdp2gh 133 4 similar similar JJ cord-022774-wasdp2gh 133 5 to to IN cord-022774-wasdp2gh 133 6 that that DT cord-022774-wasdp2gh 133 7 of of IN cord-022774-wasdp2gh 133 8 control control NN cord-022774-wasdp2gh 133 9 cell cell NN cord-022774-wasdp2gh 133 10 , , , cord-022774-wasdp2gh 133 11 . . . cord-022774-wasdp2gh 134 1 C C NNP cord-022774-wasdp2gh 134 2 , , , cord-022774-wasdp2gh 134 3 Incubation Incubation NNP cord-022774-wasdp2gh 134 4 with with IN cord-022774-wasdp2gh 134 5 I I NNP cord-022774-wasdp2gh 134 6 mM mm CD cord-022774-wasdp2gh 134 7 CoCI CoCI NNP cord-022774-wasdp2gh 134 8 , , , cord-022774-wasdp2gh 134 9 for for IN cord-022774-wasdp2gh 134 10 30 30 CD cord-022774-wasdp2gh 134 11 rain rain NN cord-022774-wasdp2gh 134 12 . . . cord-022774-wasdp2gh 135 1 Numerous numerous JJ cord-022774-wasdp2gh 135 2 tubular tubular JJ cord-022774-wasdp2gh 135 3 transition transition NN cord-022774-wasdp2gh 135 4 elements element NNS cord-022774-wasdp2gh 135 5 and and CC cord-022774-wasdp2gh 135 6 transition transition NN cord-022774-wasdp2gh 135 7 vesicles vesicle NNS cord-022774-wasdp2gh 135 8 are be VBP cord-022774-wasdp2gh 135 9 present present JJ cord-022774-wasdp2gh 135 10 in in IN cord-022774-wasdp2gh 135 11 the the DT cord-022774-wasdp2gh 135 12 Golgi Golgi NNP cord-022774-wasdp2gh 135 13 apparatus apparatus NN cord-022774-wasdp2gh 135 14 zone zone NN cord-022774-wasdp2gh 135 15 similar similar JJ cord-022774-wasdp2gh 135 16 to to IN cord-022774-wasdp2gh 135 17 those those DT cord-022774-wasdp2gh 135 18 seen see VBN cord-022774-wasdp2gh 135 19 at at IN cord-022774-wasdp2gh 135 20 16 16 CD cord-022774-wasdp2gh 135 21 ° ° NN cord-022774-wasdp2gh 135 22 C c NN cord-022774-wasdp2gh 135 23 . . . cord-022774-wasdp2gh 136 1 Bar=0.5 Bar=0.5 NNP cord-022774-wasdp2gh 137 1 ~m ~m JJ cord-022774-wasdp2gh 137 2 . . . cord-022774-wasdp2gh 138 1 similar similar JJ cord-022774-wasdp2gh 138 2 analysis analysis NN cord-022774-wasdp2gh 138 3 was be VBD cord-022774-wasdp2gh 138 4 carded card VBN cord-022774-wasdp2gh 138 5 out out RP cord-022774-wasdp2gh 138 6 with with IN cord-022774-wasdp2gh 138 7 those those DT cord-022774-wasdp2gh 138 8 vesicles vesicle NNS cord-022774-wasdp2gh 138 9 scored score VBN cord-022774-wasdp2gh 138 10 as as IN cord-022774-wasdp2gh 138 11 transition transition NN cord-022774-wasdp2gh 138 12 vesicles vesicle NNS cord-022774-wasdp2gh 138 13 in in IN cord-022774-wasdp2gh 138 14 the the DT cord-022774-wasdp2gh 138 15 in in FW cord-022774-wasdp2gh 138 16 vitro vitro FW cord-022774-wasdp2gh 138 17 incubated incubate VBN cord-022774-wasdp2gh 138 18 fractions fraction NNS cord-022774-wasdp2gh 138 19 , , , cord-022774-wasdp2gh 138 20 the the DT cord-022774-wasdp2gh 138 21 average average JJ cord-022774-wasdp2gh 138 22 diameters diameter NNS cord-022774-wasdp2gh 138 23 were be VBD cord-022774-wasdp2gh 138 24 greater great JJR cord-022774-wasdp2gh 138 25 both both DT cord-022774-wasdp2gh 138 26 at at IN cord-022774-wasdp2gh 138 27 4 4 CD cord-022774-wasdp2gh 138 28 ° ° NN cord-022774-wasdp2gh 138 29 C c NN cord-022774-wasdp2gh 138 30 and and CC cord-022774-wasdp2gh 138 31 at at IN cord-022774-wasdp2gh 138 32 37 37 CD cord-022774-wasdp2gh 138 33 ° ° NN cord-022774-wasdp2gh 138 34 C c NN cord-022774-wasdp2gh 138 35 . . . cord-022774-wasdp2gh 139 1 However however RB cord-022774-wasdp2gh 139 2 , , , cord-022774-wasdp2gh 139 3 even even RB cord-022774-wasdp2gh 139 4 in in IN cord-022774-wasdp2gh 139 5 vitro vitro FW cord-022774-wasdp2gh 139 6 , , , cord-022774-wasdp2gh 139 7 the the DT cord-022774-wasdp2gh 139 8 temperature temperature NN cord-022774-wasdp2gh 139 9 effect effect NN cord-022774-wasdp2gh 139 10 on on IN cord-022774-wasdp2gh 139 11 vesicle vesicle NN cord-022774-wasdp2gh 139 12 diameter diameter NN cord-022774-wasdp2gh 139 13 , , , cord-022774-wasdp2gh 139 14 although although IN cord-022774-wasdp2gh 139 15 small small JJ cord-022774-wasdp2gh 139 16 , , , cord-022774-wasdp2gh 139 17 was be VBD cord-022774-wasdp2gh 139 18 still still RB cord-022774-wasdp2gh 139 19 evident evident JJ cord-022774-wasdp2gh 139 20 . . . cord-022774-wasdp2gh 140 1 The the DT cord-022774-wasdp2gh 140 2 average average JJ cord-022774-wasdp2gh 140 3 vesicle vesicle NN cord-022774-wasdp2gh 140 4 diameter diameter NN cord-022774-wasdp2gh 140 5 at at IN cord-022774-wasdp2gh 140 6 4 4 CD cord-022774-wasdp2gh 140 7 ° ° NN cord-022774-wasdp2gh 141 1 C C NNP cord-022774-wasdp2gh 141 2 was be VBD cord-022774-wasdp2gh 141 3 72 72 CD cord-022774-wasdp2gh 141 4 / / SYM cord-022774-wasdp2gh 141 5 am be VBP cord-022774-wasdp2gh 141 6 while while IN cord-022774-wasdp2gh 141 7 the the DT cord-022774-wasdp2gh 141 8 average average JJ cord-022774-wasdp2gh 141 9 diameter diameter NN cord-022774-wasdp2gh 141 10 at at IN cord-022774-wasdp2gh 141 11 37 37 CD cord-022774-wasdp2gh 141 12 ° ° NN cord-022774-wasdp2gh 141 13 C C NNP cord-022774-wasdp2gh 141 14 was be VBD cord-022774-wasdp2gh 141 15 88 88 CD cord-022774-wasdp2gh 141 16 Fro Fro NNP cord-022774-wasdp2gh 141 17 . . . cord-022774-wasdp2gh 142 1 Thus thus RB cord-022774-wasdp2gh 142 2 the the DT cord-022774-wasdp2gh 142 3 response response NN cord-022774-wasdp2gh 142 4 of of IN cord-022774-wasdp2gh 142 5 transitional transitional JJ cord-022774-wasdp2gh 142 6 elements element NNS cord-022774-wasdp2gh 142 7 of of IN cord-022774-wasdp2gh 142 8 the the DT cord-022774-wasdp2gh 142 9 endoplasmic endoplasmic NN cord-022774-wasdp2gh 142 10 reticulum reticulum NN cord-022774-wasdp2gh 142 11 from from IN cord-022774-wasdp2gh 142 12 liver liver NN cord-022774-wasdp2gh 142 13 to to IN cord-022774-wasdp2gh 142 14 temperature temperature NN cord-022774-wasdp2gh 142 15 in in IN cord-022774-wasdp2gh 142 16 vitro vitro FW cord-022774-wasdp2gh 142 17 paralleled parallel VBD cord-022774-wasdp2gh 142 18 that that WDT cord-022774-wasdp2gh 142 19 observed observe VBD cord-022774-wasdp2gh 142 20 with with IN cord-022774-wasdp2gh 142 21 liver liver NN cord-022774-wasdp2gh 142 22 slices slice NNS cord-022774-wasdp2gh 142 23 in in IN cord-022774-wasdp2gh 142 24 situ situ NN cord-022774-wasdp2gh 142 25 . . . cord-022774-wasdp2gh 143 1 Temperature temperature NN cord-022774-wasdp2gh 143 2 - - HYPH cord-022774-wasdp2gh 143 3 dependent dependent JJ cord-022774-wasdp2gh 143 4 formation formation NN cord-022774-wasdp2gh 143 5 of of IN cord-022774-wasdp2gh 143 6 transition transition NN cord-022774-wasdp2gh 143 7 vesicles vesicle NNS cord-022774-wasdp2gh 143 8 occurs occur VBZ cord-022774-wasdp2gh 143 9 primarily primarily RB cord-022774-wasdp2gh 143 10 at at IN cord-022774-wasdp2gh 143 11 temperatures temperature NNS cord-022774-wasdp2gh 143 12 above above IN cord-022774-wasdp2gh 143 13 12 12 CD cord-022774-wasdp2gh 143 14 ° ° NN cord-022774-wasdp2gh 143 15 C c NN cord-022774-wasdp2gh 143 16 , , , cord-022774-wasdp2gh 143 17 becom~.ng becom~.ng NN cord-022774-wasdp2gh 143 18 significant significant JJ cord-022774-wasdp2gh 143 19 at at IN cord-022774-wasdp2gh 143 20 approximately approximately RB cord-022774-wasdp2gh 143 21 20 20 CD cord-022774-wasdp2gh 143 22 ° ° NN cord-022774-wasdp2gh 143 23 C c NN cord-022774-wasdp2gh 143 24 . . . cord-022774-wasdp2gh 144 1 The the DT cord-022774-wasdp2gh 144 2 vesicle vesicle NN cord-022774-wasdp2gh 144 3 diameter diameter NN cord-022774-wasdp2gh 144 4 was be VBD cord-022774-wasdp2gh 144 5 greater great JJR cord-022774-wasdp2gh 144 6 at at IN cord-022774-wasdp2gh 144 7 37 37 CD cord-022774-wasdp2gh 144 8 ° ° NN cord-022774-wasdp2gh 144 9 C c NN cord-022774-wasdp2gh 144 10 than than IN cord-022774-wasdp2gh 144 11 at at IN cord-022774-wasdp2gh 144 12 4 4 CD cord-022774-wasdp2gh 144 13 ° ° NN cord-022774-wasdp2gh 144 14 C c NN cord-022774-wasdp2gh 144 15 both both CC cord-022774-wasdp2gh 144 16 in in IN cord-022774-wasdp2gh 144 17 situ situ NN cord-022774-wasdp2gh 144 18 and and CC cord-022774-wasdp2gh 144 19 in in IN cord-022774-wasdp2gh 144 20 vitro vitro FW cord-022774-wasdp2gh 144 21 with with IN cord-022774-wasdp2gh 144 22 the the DT cord-022774-wasdp2gh 144 23 isolated isolated JJ cord-022774-wasdp2gh 144 24 fractions fraction NNS cord-022774-wasdp2gh 144 25 . . . cord-022774-wasdp2gh 145 1 A a DT cord-022774-wasdp2gh 145 2 16 16 CD cord-022774-wasdp2gh 145 3 ° ° NN cord-022774-wasdp2gh 145 4 C c NN cord-022774-wasdp2gh 145 5 compartment compartment NN cord-022774-wasdp2gh 145 6 intermediate intermediate NN cord-022774-wasdp2gh 145 7 between between IN cord-022774-wasdp2gh 145 8 the the DT cord-022774-wasdp2gh 145 9 endoplasmic endoplasmic NN cord-022774-wasdp2gh 145 10 reticulum reticulum NN cord-022774-wasdp2gh 145 11 and and CC cord-022774-wasdp2gh 145 12 the the DT cord-022774-wasdp2gh 145 13 Golgi Golgi NNP cord-022774-wasdp2gh 145 14 apparatus apparatus NN cord-022774-wasdp2gh 145 15 has have VBZ cord-022774-wasdp2gh 145 16 been be VBN cord-022774-wasdp2gh 145 17 reported report VBN cord-022774-wasdp2gh 145 18 for for IN cord-022774-wasdp2gh 145 19 cultured cultured JJ cord-022774-wasdp2gh 145 20 cells cell NNS cord-022774-wasdp2gh 145 21 infected infect VBN cord-022774-wasdp2gh 145 22 with with IN cord-022774-wasdp2gh 145 23 temperature temperature NN cord-022774-wasdp2gh 145 24 sensitive sensitive JJ cord-022774-wasdp2gh 145 25 virus virus NN cord-022774-wasdp2gh 145 26 strains strain NNS cord-022774-wasdp2gh 145 27 [ [ -LRB- cord-022774-wasdp2gh 145 28 8 8 CD cord-022774-wasdp2gh 145 29 , , , cord-022774-wasdp2gh 145 30 17 17 CD cord-022774-wasdp2gh 145 31 , , , cord-022774-wasdp2gh 145 32 20 20 CD cord-022774-wasdp2gh 145 33 , , , cord-022774-wasdp2gh 145 34 21 21 CD cord-022774-wasdp2gh 145 35 ] ] -RRB- cord-022774-wasdp2gh 145 36 . . . cord-022774-wasdp2gh 146 1 In in IN cord-022774-wasdp2gh 146 2 order order NN cord-022774-wasdp2gh 146 3 to to TO cord-022774-wasdp2gh 146 4 attempt attempt VB cord-022774-wasdp2gh 146 5 to to TO cord-022774-wasdp2gh 146 6 define define VB cord-022774-wasdp2gh 146 7 a a DT cord-022774-wasdp2gh 146 8 16 16 CD cord-022774-wasdp2gh 146 9 ° ° NN cord-022774-wasdp2gh 146 10 C c NN cord-022774-wasdp2gh 146 11 compartment compartment NN cord-022774-wasdp2gh 146 12 for for IN cord-022774-wasdp2gh 146 13 liver liver NN cord-022774-wasdp2gh 146 14 , , , cord-022774-wasdp2gh 146 15 parallel parallel JJ cord-022774-wasdp2gh 146 16 experiments experiment NNS cord-022774-wasdp2gh 146 17 were be VBD cord-022774-wasdp2gh 146 18 carried carry VBN cord-022774-wasdp2gh 146 19 out out RP cord-022774-wasdp2gh 146 20 with with IN cord-022774-wasdp2gh 146 21 BHK BHK NNP cord-022774-wasdp2gh 146 22 cells cell NNS cord-022774-wasdp2gh 146 23 incubated incubate VBN cord-022774-wasdp2gh 146 24 at at IN cord-022774-wasdp2gh 146 25 different different JJ cord-022774-wasdp2gh 146 26 temperatures temperature NNS cord-022774-wasdp2gh 146 27 in in IN cord-022774-wasdp2gh 146 28 normal normal JJ cord-022774-wasdp2gh 146 29 culture culture NN cord-022774-wasdp2gh 146 30 medium medium NN cord-022774-wasdp2gh 146 31 . . . cord-022774-wasdp2gh 147 1 As as IN cord-022774-wasdp2gh 147 2 with with IN cord-022774-wasdp2gh 147 3 liver liver NN cord-022774-wasdp2gh 147 4 cells cell NNS cord-022774-wasdp2gh 147 5 , , , cord-022774-wasdp2gh 147 6 both both CC cord-022774-wasdp2gh 147 7 at at IN cord-022774-wasdp2gh 147 8 16 16 CD cord-022774-wasdp2gh 147 9 ° ° NN cord-022774-wasdp2gh 147 10 C c NN cord-022774-wasdp2gh 147 11 and and CC cord-022774-wasdp2gh 147 12 at at IN cord-022774-wasdp2gh 147 13 18 18 CD cord-022774-wasdp2gh 147 14 ° ° NN cord-022774-wasdp2gh 147 15 C c NN cord-022774-wasdp2gh 147 16 , , , cord-022774-wasdp2gh 147 17 transition transition NN cord-022774-wasdp2gh 147 18 vesicles vesicle NNS cord-022774-wasdp2gh 147 19 accumulated accumulate VBN cord-022774-wasdp2gh 147 20 . . . cord-022774-wasdp2gh 148 1 However however RB cord-022774-wasdp2gh 148 2 , , , cord-022774-wasdp2gh 148 3 at at IN cord-022774-wasdp2gh 148 4 temperatures temperature NNS cord-022774-wasdp2gh 148 5 of of IN cord-022774-wasdp2gh 148 6 18 18 CD cord-022774-wasdp2gh 148 7 ° ° NN cord-022774-wasdp2gh 148 8 C c NN cord-022774-wasdp2gh 148 9 and and CC cord-022774-wasdp2gh 148 10 below below RB cord-022774-wasdp2gh 148 11 , , , cord-022774-wasdp2gh 148 12 there there EX cord-022774-wasdp2gh 148 13 was be VBD cord-022774-wasdp2gh 148 14 also also RB cord-022774-wasdp2gh 148 15 an an DT cord-022774-wasdp2gh 148 16 accumulation accumulation NN cord-022774-wasdp2gh 148 17 of of IN cord-022774-wasdp2gh 148 18 smooth smooth JJ cord-022774-wasdp2gh 148 19 tubular tubular JJ cord-022774-wasdp2gh 148 20 elements element NNS cord-022774-wasdp2gh 148 21 of of IN cord-022774-wasdp2gh 148 22 the the DT cord-022774-wasdp2gh 148 23 endoplasmic endoplasmic NN cord-022774-wasdp2gh 148 24 reticulum reticulum NN cord-022774-wasdp2gh 148 25 in in IN cord-022774-wasdp2gh 148 26 the the DT cord-022774-wasdp2gh 148 27 peripheral peripheral JJ cord-022774-wasdp2gh 148 28 cytoplasm cytoplasm NN cord-022774-wasdp2gh 148 29 surrounding surround VBG cord-022774-wasdp2gh 148 30 the the DT cord-022774-wasdp2gh 148 31 Golgi Golgi NNP cord-022774-wasdp2gh 148 32 apparatus apparatus NN cord-022774-wasdp2gh 148 33 most most RBS cord-022774-wasdp2gh 148 34 often often RB cord-022774-wasdp2gh 148 35 in in IN cord-022774-wasdp2gh 148 36 near near JJ cord-022774-wasdp2gh 148 37 proximity proximity NN cord-022774-wasdp2gh 148 38 to to IN cord-022774-wasdp2gh 148 39 the the DT cord-022774-wasdp2gh 148 40 cis cis NNP cord-022774-wasdp2gh 148 41 face face NN cord-022774-wasdp2gh 148 42 . . . cord-022774-wasdp2gh 149 1 We -PRON- PRP cord-022774-wasdp2gh 149 2 suggest suggest VBP cord-022774-wasdp2gh 149 3 this this DT cord-022774-wasdp2gh 149 4 structure structure NN cord-022774-wasdp2gh 149 5 to to TO cord-022774-wasdp2gh 149 6 be be VB cord-022774-wasdp2gh 149 7 morphologically morphologically RB cord-022774-wasdp2gh 149 8 and and CC cord-022774-wasdp2gh 149 9 structurally structurally RB cord-022774-wasdp2gh 149 10 homologous homologous JJ cord-022774-wasdp2gh 149 11 to to IN cord-022774-wasdp2gh 149 12 the the DT cord-022774-wasdp2gh 149 13 16 16 CD cord-022774-wasdp2gh 149 14 ° ° NN cord-022774-wasdp2gh 149 15 C C NNP cord-022774-wasdp2gh 149 16 compartment compartment NN cord-022774-wasdp2gh 149 17 of of IN cord-022774-wasdp2gh 149 18 virus virus NN cord-022774-wasdp2gh 149 19 - - HYPH cord-022774-wasdp2gh 149 20 infected infect VBN cord-022774-wasdp2gh 149 21 cells cell NNS cord-022774-wasdp2gh 149 22 [ [ -LRB- cord-022774-wasdp2gh 149 23 21 21 CD cord-022774-wasdp2gh 149 24 ] ] -RRB- cord-022774-wasdp2gh 149 25 . . . cord-022774-wasdp2gh 150 1 The the DT cord-022774-wasdp2gh 150 2 smooth smooth JJ cord-022774-wasdp2gh 150 3 tubular tubular JJ cord-022774-wasdp2gh 150 4 endoplasmic endoplasmic NN cord-022774-wasdp2gh 150 5 reticulum reticulum NN cord-022774-wasdp2gh 150 6 elements element NNS cord-022774-wasdp2gh 150 7 seen see VBN cord-022774-wasdp2gh 150 8 with with IN cord-022774-wasdp2gh 150 9 liver liver NN cord-022774-wasdp2gh 150 10 slices slice NNS cord-022774-wasdp2gh 150 11 at at IN cord-022774-wasdp2gh 150 12 temperatures temperature NNS cord-022774-wasdp2gh 150 13 of of IN cord-022774-wasdp2gh 150 14 18 18 CD cord-022774-wasdp2gh 150 15 ° ° NN cord-022774-wasdp2gh 150 16 C c NN cord-022774-wasdp2gh 150 17 and and CC cord-022774-wasdp2gh 150 18 below below RB cord-022774-wasdp2gh 150 19 and and CC cord-022774-wasdp2gh 150 20 especially especially RB cord-022774-wasdp2gh 150 21 at at IN cord-022774-wasdp2gh 150 22 8 8 CD cord-022774-wasdp2gh 150 23 ° ° NN cord-022774-wasdp2gh 150 24 C c NN cord-022774-wasdp2gh 150 25 and and CC cord-022774-wasdp2gh 150 26 12 12 CD cord-022774-wasdp2gh 150 27 ° ° NN cord-022774-wasdp2gh 150 28 C c NN cord-022774-wasdp2gh 150 29 also also RB cord-022774-wasdp2gh 150 30 appear appear VBP cord-022774-wasdp2gh 150 31 to to TO cord-022774-wasdp2gh 150 32 be be VB cord-022774-wasdp2gh 150 33 structurally structurally RB cord-022774-wasdp2gh 150 34 homologous homologous JJ cord-022774-wasdp2gh 150 35 to to IN cord-022774-wasdp2gh 150 36 the the DT cord-022774-wasdp2gh 150 37 16 16 CD cord-022774-wasdp2gh 150 38 ° ° NN cord-022774-wasdp2gh 150 39 C c NN cord-022774-wasdp2gh 150 40 compartment compartment NN cord-022774-wasdp2gh 150 41 seen see VBN cord-022774-wasdp2gh 150 42 in in IN cord-022774-wasdp2gh 150 43 cultured cultured JJ cord-022774-wasdp2gh 150 44 cells cell NNS cord-022774-wasdp2gh 150 45 . . . cord-022774-wasdp2gh 151 1 While while IN cord-022774-wasdp2gh 151 2 the the DT cord-022774-wasdp2gh 151 3 formation formation NN cord-022774-wasdp2gh 151 4 of of IN cord-022774-wasdp2gh 151 5 a a DT cord-022774-wasdp2gh 151 6 16 16 CD cord-022774-wasdp2gh 151 7 ° ° NN cord-022774-wasdp2gh 151 8 C c NN cord-022774-wasdp2gh 151 9 compartment compartment NN cord-022774-wasdp2gh 151 10 by by IN cord-022774-wasdp2gh 151 11 the the DT cord-022774-wasdp2gh 151 12 isolated isolate VBN cord-022774-wasdp2gh 151 13 membrane membrane NN cord-022774-wasdp2gh 151 14 fractions fraction NNS cord-022774-wasdp2gh 151 15 is be VBZ cord-022774-wasdp2gh 151 16 not not RB cord-022774-wasdp2gh 151 17 immediately immediately RB cord-022774-wasdp2gh 151 18 obvious obvious JJ cord-022774-wasdp2gh 151 19 , , , cord-022774-wasdp2gh 151 20 preliminary preliminary JJ cord-022774-wasdp2gh 151 21 indications indication NNS cord-022774-wasdp2gh 151 22 are be VBP cord-022774-wasdp2gh 151 23 that that IN cord-022774-wasdp2gh 151 24 the the DT cord-022774-wasdp2gh 151 25 stalked stalk VBN cord-022774-wasdp2gh 151 26 vesicles vesicle NNS cord-022774-wasdp2gh 151 27 or or CC cord-022774-wasdp2gh 151 28 that that IN cord-022774-wasdp2gh 151 29 some some DT cord-022774-wasdp2gh 151 30 of of IN cord-022774-wasdp2gh 151 31 the the DT cord-022774-wasdp2gh 151 32 membrane membrane NN cord-022774-wasdp2gh 151 33 blebs blebs NN cord-022774-wasdp2gh 151 34 still still RB cord-022774-wasdp2gh 151 35 attached attach VBN cord-022774-wasdp2gh 151 36 to to IN cord-022774-wasdp2gh 151 37 endoplasmic endoplasmic VB cord-022774-wasdp2gh 151 38 reticulum reticulum NN cord-022774-wasdp2gh 151 39 fragments fragment NNS cord-022774-wasdp2gh 151 40 seen see VBN cord-022774-wasdp2gh 151 41 in in IN cord-022774-wasdp2gh 151 42 isolated isolated JJ cord-022774-wasdp2gh 151 43 preparations preparation NNS cord-022774-wasdp2gh 151 44 exposed expose VBN cord-022774-wasdp2gh 151 45 to to IN cord-022774-wasdp2gh 151 46 ATP ATP NNP cord-022774-wasdp2gh 151 47 and and CC cord-022774-wasdp2gh 151 48 cytosol cytosol NN cord-022774-wasdp2gh 151 49 but but CC cord-022774-wasdp2gh 151 50 not not RB cord-022774-wasdp2gh 151 51 incubated incubate VBN cord-022774-wasdp2gh 151 52 at at IN cord-022774-wasdp2gh 151 53 temperatures temperature NNS cord-022774-wasdp2gh 151 54 of of IN cord-022774-wasdp2gh 151 55 16 16 CD cord-022774-wasdp2gh 151 56 ° ° NN cord-022774-wasdp2gh 151 57 C c NN cord-022774-wasdp2gh 151 58 and and CC cord-022774-wasdp2gh 151 59 above above RB cord-022774-wasdp2gh 151 60 may may MD cord-022774-wasdp2gh 151 61 be be VB cord-022774-wasdp2gh 151 62 the the DT cord-022774-wasdp2gh 151 63 equivalent equivalent JJ cord-022774-wasdp2gh 151 64 structures structure NNS cord-022774-wasdp2gh 151 65 . . . cord-022774-wasdp2gh 152 1 Both both DT cord-022774-wasdp2gh 152 2 cyanide cyanide NN cord-022774-wasdp2gh 152 3 and and CC cord-022774-wasdp2gh 152 4 cobalt cobalt NN cord-022774-wasdp2gh 152 5 have have VBP cord-022774-wasdp2gh 152 6 been be VBN cord-022774-wasdp2gh 152 7 reported report VBN cord-022774-wasdp2gh 152 8 to to TO cord-022774-wasdp2gh 152 9 inhibit inhibit VB cord-022774-wasdp2gh 152 10 the the DT cord-022774-wasdp2gh 152 11 transfer transfer NN cord-022774-wasdp2gh 152 12 step step NN cord-022774-wasdp2gh 152 13 between between IN cord-022774-wasdp2gh 152 14 endoplasmic endoplasmic NN cord-022774-wasdp2gh 152 15 reticulum reticulum NN cord-022774-wasdp2gh 152 16 and and CC cord-022774-wasdp2gh 152 17 Golgi Golgi NNP cord-022774-wasdp2gh 152 18 apparatus apparatus NN cord-022774-wasdp2gh 152 19 [ [ -LRB- cord-022774-wasdp2gh 152 20 9 9 CD cord-022774-wasdp2gh 152 21 ] ] -RRB- cord-022774-wasdp2gh 152 22 . . . cord-022774-wasdp2gh 153 1 Cyanide cyanide NN cord-022774-wasdp2gh 153 2 presumably presumably RB cord-022774-wasdp2gh 153 3 blocks block VBZ cord-022774-wasdp2gh 153 4 through through IN cord-022774-wasdp2gh 153 5 its -PRON- PRP$ cord-022774-wasdp2gh 153 6 ability ability NN cord-022774-wasdp2gh 153 7 to to TO cord-022774-wasdp2gh 153 8 deplete deplete VB cord-022774-wasdp2gh 153 9 the the DT cord-022774-wasdp2gh 153 10 energy energy NN cord-022774-wasdp2gh 153 11 charge charge NN cord-022774-wasdp2gh 153 12 of of IN cord-022774-wasdp2gh 153 13 the the DT cord-022774-wasdp2gh 153 14 cell cell NN cord-022774-wasdp2gh 153 15 whereas whereas IN cord-022774-wasdp2gh 153 16 how how WRB cord-022774-wasdp2gh 153 17 cobalt cobalt NN cord-022774-wasdp2gh 153 18 inhibits inhibit VBZ cord-022774-wasdp2gh 153 19 is be VBZ cord-022774-wasdp2gh 153 20 not not RB cord-022774-wasdp2gh 153 21 known know VBN cord-022774-wasdp2gh 153 22 . . . cord-022774-wasdp2gh 154 1 With with IN cord-022774-wasdp2gh 154 2 BHK BHK NNP cord-022774-wasdp2gh 154 3 ceils ceil NNS cord-022774-wasdp2gh 154 4 , , , cord-022774-wasdp2gh 154 5 cyanide cyanide NN cord-022774-wasdp2gh 154 6 did do VBD cord-022774-wasdp2gh 154 7 not not RB cord-022774-wasdp2gh 154 8 result result VB cord-022774-wasdp2gh 154 9 in in IN cord-022774-wasdp2gh 154 10 vesicle vesicle NN cord-022774-wasdp2gh 154 11 accumulations accumulation NNS cord-022774-wasdp2gh 154 12 . . . cord-022774-wasdp2gh 155 1 At at IN cord-022774-wasdp2gh 155 2 least least JJS cord-022774-wasdp2gh 155 3 in in IN cord-022774-wasdp2gh 155 4 the the DT cord-022774-wasdp2gh 155 5 cell cell NN cord-022774-wasdp2gh 155 6 - - HYPH cord-022774-wasdp2gh 155 7 free free JJ cord-022774-wasdp2gh 155 8 [ [ -LRB- cord-022774-wasdp2gh 155 9 16 16 CD cord-022774-wasdp2gh 155 10 ] ] -RRB- cord-022774-wasdp2gh 155 11 and and CC cord-022774-wasdp2gh 155 12 perforated perforate VBD cord-022774-wasdp2gh 155 13 [ [ -LRB- cord-022774-wasdp2gh 155 14 1 1 CD cord-022774-wasdp2gh 155 15 , , , cord-022774-wasdp2gh 155 16 2 2 CD cord-022774-wasdp2gh 155 17 ] ] -RRB- cord-022774-wasdp2gh 155 18 cell cell NN cord-022774-wasdp2gh 155 19 systems system NNS cord-022774-wasdp2gh 155 20 , , , cord-022774-wasdp2gh 155 21 vesicle vesicle NN cord-022774-wasdp2gh 155 22 formation formation NN cord-022774-wasdp2gh 155 23 and and CC cord-022774-wasdp2gh 155 24 transfer transfer NN cord-022774-wasdp2gh 155 25 shows show VBZ cord-022774-wasdp2gh 155 26 an an DT cord-022774-wasdp2gh 155 27 absolute absolute JJ cord-022774-wasdp2gh 155 28 energy energy NN cord-022774-wasdp2gh 155 29 dependence dependence NN cord-022774-wasdp2gh 155 30 . . . cord-022774-wasdp2gh 156 1 Thus thus RB cord-022774-wasdp2gh 156 2 , , , cord-022774-wasdp2gh 156 3 an an DT cord-022774-wasdp2gh 156 4 accumulation accumulation NN cord-022774-wasdp2gh 156 5 of of IN cord-022774-wasdp2gh 156 6 vesicles vesicle NNS cord-022774-wasdp2gh 156 7 under under IN cord-022774-wasdp2gh 156 8 conditions condition NNS cord-022774-wasdp2gh 156 9 of of IN cord-022774-wasdp2gh 156 10 energy energy NN cord-022774-wasdp2gh 156 11 depletion depletion NN cord-022774-wasdp2gh 156 12 would would MD cord-022774-wasdp2gh 156 13 not not RB cord-022774-wasdp2gh 156 14 be be VB cord-022774-wasdp2gh 156 15 expected expect VBN cord-022774-wasdp2gh 156 16 . . . cord-022774-wasdp2gh 157 1 In in IN cord-022774-wasdp2gh 157 2 contrast contrast NN cord-022774-wasdp2gh 157 3 to to IN cord-022774-wasdp2gh 157 4 cyanide cyanide NN cord-022774-wasdp2gh 157 5 , , , cord-022774-wasdp2gh 157 6 the the DT cord-022774-wasdp2gh 157 7 cobalt cobalt NN cord-022774-wasdp2gh 157 8 treatment treatment NN cord-022774-wasdp2gh 157 9 resulted result VBD cord-022774-wasdp2gh 157 10 in in IN cord-022774-wasdp2gh 157 11 increased increase VBN cord-022774-wasdp2gh 157 12 numbers number NNS cord-022774-wasdp2gh 157 13 of of IN cord-022774-wasdp2gh 157 14 transition transition NN cord-022774-wasdp2gh 157 15 vesicles vesicle NNS cord-022774-wasdp2gh 157 16 with with IN cord-022774-wasdp2gh 157 17 the the DT cord-022774-wasdp2gh 157 18 BHK BHK NNP cord-022774-wasdp2gh 157 19 cells cell NNS cord-022774-wasdp2gh 157 20 rcsembling rcsemble VBG cord-022774-wasdp2gh 157 21 the the DT cord-022774-wasdp2gh 157 22 response response NN cord-022774-wasdp2gh 157 23 to to IN cord-022774-wasdp2gh 157 24 16 16 CD cord-022774-wasdp2gh 157 25 ° ° NN cord-022774-wasdp2gh 157 26 C c NN cord-022774-wasdp2gh 157 27 . . . cord-022774-wasdp2gh 158 1 Similar similar JJ cord-022774-wasdp2gh 158 2 results result NNS cord-022774-wasdp2gh 158 3 could could MD cord-022774-wasdp2gh 158 4 not not RB cord-022774-wasdp2gh 158 5 be be VB cord-022774-wasdp2gh 158 6 shown show VBN cord-022774-wasdp2gh 158 7 with with IN cord-022774-wasdp2gh 158 8 liver liver NN cord-022774-wasdp2gh 158 9 due due IN cord-022774-wasdp2gh 158 10 to to IN cord-022774-wasdp2gh 158 11 the the DT cord-022774-wasdp2gh 158 12 extreme extreme JJ cord-022774-wasdp2gh 158 13 cytotoxicity cytotoxicity NN cord-022774-wasdp2gh 158 14 of of IN cord-022774-wasdp2gh 158 15 liver liver NN cord-022774-wasdp2gh 158 16 slices slice NNS cord-022774-wasdp2gh 158 17 to to IN cord-022774-wasdp2gh 158 18 the the DT cord-022774-wasdp2gh 158 19 same same JJ cord-022774-wasdp2gh 158 20 cobalt cobalt NN cord-022774-wasdp2gh 158 21 concentrations concentration NNS cord-022774-wasdp2gh 158 22 and and CC cord-022774-wasdp2gh 158 23 treatment treatment NN cord-022774-wasdp2gh 158 24 conditions condition NNS cord-022774-wasdp2gh 158 25 that that WDT cord-022774-wasdp2gh 158 26 resulted result VBD cord-022774-wasdp2gh 158 27 in in IN cord-022774-wasdp2gh 158 28 transition transition NN cord-022774-wasdp2gh 158 29 vesicle vesicle NN cord-022774-wasdp2gh 158 30 accumulations accumulation NNS cord-022774-wasdp2gh 158 31 with with IN cord-022774-wasdp2gh 158 32 tile tile NN cord-022774-wasdp2gh 158 33 cultured cultured JJ cord-022774-wasdp2gh 158 34 cells cell NNS cord-022774-wasdp2gh 158 35 . . . cord-022774-wasdp2gh 159 1 Cobalt cobalt NN cord-022774-wasdp2gh 159 2 ions ion NNS cord-022774-wasdp2gh 159 3 were be VBD cord-022774-wasdp2gh 159 4 selected select VBN cord-022774-wasdp2gh 159 5 initially initially RB cord-022774-wasdp2gh 159 6 for for IN cord-022774-wasdp2gh 159 7 comparison comparison NN cord-022774-wasdp2gh 159 8 to to IN cord-022774-wasdp2gh 159 9 low low JJ cord-022774-wasdp2gh 159 10 temperature temperature NN cord-022774-wasdp2gh 159 11 because because IN cord-022774-wasdp2gh 159 12 of of IN cord-022774-wasdp2gh 159 13 their -PRON- PRP$ cord-022774-wasdp2gh 159 14 reported report VBN cord-022774-wasdp2gh 159 15 ability ability NN cord-022774-wasdp2gh 159 16 at at IN cord-022774-wasdp2gh 159 17 1 1 CD cord-022774-wasdp2gh 159 18 mM mm CD cord-022774-wasdp2gh 159 19 to to TO cord-022774-wasdp2gh 159 20 inhibit inhibit VB cord-022774-wasdp2gh 159 21 fast fast JJ cord-022774-wasdp2gh 159 22 axonal axonal JJ cord-022774-wasdp2gh 159 23 transport transport NN cord-022774-wasdp2gh 159 24 [ [ -LRB- cord-022774-wasdp2gh 159 25 7 7 CD cord-022774-wasdp2gh 159 26 ] ] -RRB- cord-022774-wasdp2gh 159 27 . . . cord-022774-wasdp2gh 160 1 Previous previous JJ cord-022774-wasdp2gh 160 2 findings finding NNS cord-022774-wasdp2gh 160 3 suggested suggest VBD cord-022774-wasdp2gh 160 4 that that IN cord-022774-wasdp2gh 160 5 cobalt cobalt NN cord-022774-wasdp2gh 160 6 was be VBD cord-022774-wasdp2gh 160 7 acting act VBG cord-022774-wasdp2gh 160 8 at at IN cord-022774-wasdp2gh 160 9 a a DT cord-022774-wasdp2gh 160 10 rate rate NN cord-022774-wasdp2gh 160 11 - - HYPH cord-022774-wasdp2gh 160 12 limiting limit VBG cord-022774-wasdp2gh 160 13 step step NN cord-022774-wasdp2gh 160 14 in in IN cord-022774-wasdp2gh 160 15 the the DT cord-022774-wasdp2gh 160 16 initiation initiation NN cord-022774-wasdp2gh 160 17 of of IN cord-022774-wasdp2gh 160 18 axonal axonal JJ cord-022774-wasdp2gh 160 19 transport transport NN cord-022774-wasdp2gh 160 20 perhaps perhaps RB cord-022774-wasdp2gh 160 21 at at IN cord-022774-wasdp2gh 160 22 a a DT cord-022774-wasdp2gh 160 23 site site NN cord-022774-wasdp2gh 160 24 where where WRB cord-022774-wasdp2gh 160 25 calcium calcium NN cord-022774-wasdp2gh 160 26 or or CC cord-022774-wasdp2gh 160 27 zinc zinc NN cord-022774-wasdp2gh 160 28 might may MD cord-022774-wasdp2gh 160 29 be be VB cord-022774-wasdp2gh 160 30 involved involve VBN cord-022774-wasdp2gh 160 31 in in IN cord-022774-wasdp2gh 160 32 the the DT cord-022774-wasdp2gh 160 33 initial initial JJ cord-022774-wasdp2gh 160 34 coupling coupling NN cord-022774-wasdp2gh 160 35 of of IN cord-022774-wasdp2gh 160 36 protein protein NN cord-022774-wasdp2gh 160 37 to to IN cord-022774-wasdp2gh 160 38 the the DT cord-022774-wasdp2gh 160 39 transport transport NN cord-022774-wasdp2gh 160 40 . . . cord-022774-wasdp2gh 161 1 Reconstitution reconstitution NN cord-022774-wasdp2gh 161 2 of of IN cord-022774-wasdp2gh 161 3 transport transport NN cord-022774-wasdp2gh 161 4 from from IN cord-022774-wasdp2gh 161 5 the the DT cord-022774-wasdp2gh 161 6 endoplasmic endoplasmic NN cord-022774-wasdp2gh 161 7 reticulum reticulum NN cord-022774-wasdp2gh 161 8 to to IN cord-022774-wasdp2gh 161 9 the the DT cord-022774-wasdp2gh 161 10 Golgi Golgi NNP cord-022774-wasdp2gh 161 11 complex complex NN cord-022774-wasdp2gh 161 12 using use VBG cord-022774-wasdp2gh 161 13 semi semi JJ cord-022774-wasdp2gh 161 14 - - JJ cord-022774-wasdp2gh 161 15 intact intact JJ cord-022774-wasdp2gh 161 16 ceils ceil NNS cord-022774-wasdp2gh 161 17 Semiintact Semiintact NNP cord-022774-wasdp2gh 161 18 cells cell NNS cord-022774-wasdp2gh 161 19 permeable permeable JJ cord-022774-wasdp2gh 161 20 to to IN cord-022774-wasdp2gh 161 21 macromolecules macromolecule NNS cord-022774-wasdp2gh 161 22 : : : cord-022774-wasdp2gh 161 23 use use VB cord-022774-wasdp2gh 161 24 in in IN cord-022774-wasdp2gh 161 25 reconstitution reconstitution NN cord-022774-wasdp2gh 161 26 of of IN cord-022774-wasdp2gh 161 27 protein protein NN cord-022774-wasdp2gh 161 28 transport transport NN cord-022774-wasdp2gh 161 29 from from IN cord-022774-wasdp2gh 161 30 the the DT cord-022774-wasdp2gh 161 31 endoplasmic endoplasmic NN cord-022774-wasdp2gh 161 32 reticulum reticulum NN cord-022774-wasdp2gh 161 33 to to IN cord-022774-wasdp2gh 161 34 the the DT cord-022774-wasdp2gh 161 35 Golgi Golgi NNP cord-022774-wasdp2gh 161 36 complex complex JJ cord-022774-wasdp2gh 161 37 Effect effect NN cord-022774-wasdp2gh 161 38 of of IN cord-022774-wasdp2gh 161 39 reduced reduce VBN cord-022774-wasdp2gh 161 40 temperature temperature NN cord-022774-wasdp2gh 161 41 on on IN cord-022774-wasdp2gh 161 42 glycoprotein glycoprotein NN cord-022774-wasdp2gh 161 43 ( ( -LRB- cord-022774-wasdp2gh 161 44 Ig Ig NNP cord-022774-wasdp2gh 161 45 , , , cord-022774-wasdp2gh 161 46 HLA HLA NNP cord-022774-wasdp2gh 161 47 ) ) -RRB- cord-022774-wasdp2gh 161 48 processing processing NN cord-022774-wasdp2gh 161 49 and and CC cord-022774-wasdp2gh 161 50 transport transport NN cord-022774-wasdp2gh 161 51 in in IN cord-022774-wasdp2gh 161 52 lymphoid lymphoid JJ cord-022774-wasdp2gh 161 53 cells cell NNS cord-022774-wasdp2gh 161 54 Folding folding NN cord-022774-wasdp2gh 161 55 , , , cord-022774-wasdp2gh 161 56 trimerization trimerization NN cord-022774-wasdp2gh 161 57 and and CC cord-022774-wasdp2gh 161 58 transport transport NN cord-022774-wasdp2gh 161 59 are be VBP cord-022774-wasdp2gh 161 60 sequential sequential JJ cord-022774-wasdp2gh 161 61 events event NNS cord-022774-wasdp2gh 161 62 in in IN cord-022774-wasdp2gh 161 63 the the DT cord-022774-wasdp2gh 161 64 biogenesis biogenesis NN cord-022774-wasdp2gh 161 65 of of IN cord-022774-wasdp2gh 161 66 influenza influenza NN cord-022774-wasdp2gh 161 67 virus virus NN cord-022774-wasdp2gh 161 68 hemagglutinin hemagglutinin NNP cord-022774-wasdp2gh 162 1 The the DT cord-022774-wasdp2gh 162 2 effects effect NNS cord-022774-wasdp2gh 162 3 of of IN cord-022774-wasdp2gh 162 4 low low JJ cord-022774-wasdp2gh 162 5 teml teml NN cord-022774-wasdp2gh 162 6 ~ ~ NFP cord-022774-wasdp2gh 162 7 ratures rature VBZ cord-022774-wasdp2gh 162 8 on on IN cord-022774-wasdp2gh 162 9 intracellular intracellular JJ cord-022774-wasdp2gh 162 10 transport transport NN cord-022774-wasdp2gh 162 11 of of IN cord-022774-wasdp2gh 162 12 newly newly RB cord-022774-wasdp2gh 162 13 synthesized synthesize VBN cord-022774-wasdp2gh 162 14 albumin albumin NN cord-022774-wasdp2gh 162 15 and and CC cord-022774-wasdp2gh 162 16 haptoglobin haptoglobin NN cord-022774-wasdp2gh 162 17 in in IN cord-022774-wasdp2gh 162 18 rat rat NN cord-022774-wasdp2gh 162 19 hepatocytes hepatocyte NNS cord-022774-wasdp2gh 162 20 Exit exit NN cord-022774-wasdp2gh 162 21 of of IN cord-022774-wasdp2gh 162 22 newly newly RB cord-022774-wasdp2gh 162 23 synthesized synthesize VBN cord-022774-wasdp2gh 162 24 membrane membrane NN cord-022774-wasdp2gh 162 25 proteins protein NNS cord-022774-wasdp2gh 162 26 from from IN cord-022774-wasdp2gh 162 27 the the DT cord-022774-wasdp2gh 162 28 transcisterna transcisterna NNP cord-022774-wasdp2gh 162 29 of of IN cord-022774-wasdp2gh 162 30 the the DT cord-022774-wasdp2gh 162 31 Golgi Golgi NNP cord-022774-wasdp2gh 162 32 complex complex NN cord-022774-wasdp2gh 162 33 to to IN cord-022774-wasdp2gh 162 34 the the DT cord-022774-wasdp2gh 162 35 plasma plasma NN cord-022774-wasdp2gh 162 36 membrane membrane NN cord-022774-wasdp2gh 162 37 Inhibition inhibition NN cord-022774-wasdp2gh 162 38 of of IN cord-022774-wasdp2gh 162 39 fast fast JJ cord-022774-wasdp2gh 162 40 axonal axonal JJ cord-022774-wasdp2gh 162 41 transport transport NN cord-022774-wasdp2gh 162 42 of of IN cord-022774-wasdp2gh 162 43 [ [ -LRB- cord-022774-wasdp2gh 162 44 ~H]protein ~H]protein NNP cord-022774-wasdp2gh 162 45 by by IN cord-022774-wasdp2gh 162 46 cobalt cobalt NN cord-022774-wasdp2gh 162 47 ions ion NNS cord-022774-wasdp2gh 163 1 Tunicamycin Tunicamycin NNP cord-022774-wasdp2gh 163 2 resistant resistant JJ cord-022774-wasdp2gh 163 3 glycosylation glycosylation NN cord-022774-wasdp2gh 163 4 of of IN cord-022774-wasdp2gh 163 5 a a DT cord-022774-wasdp2gh 163 6 coronavirus coronavirus NN cord-022774-wasdp2gh 163 7 glycoprotein glycoprotein NN cord-022774-wasdp2gh 163 8 : : : cord-022774-wasdp2gh 163 9 determination determination NN cord-022774-wasdp2gh 163 10 of of IN cord-022774-wasdp2gh 163 11 a a DT cord-022774-wasdp2gh 163 12 novel novel JJ cord-022774-wasdp2gh 163 13 type type NN cord-022774-wasdp2gh 163 14 of of IN cord-022774-wasdp2gh 163 15 viral viral JJ cord-022774-wasdp2gh 163 16 glycoprotein glycoprotein NN cord-022774-wasdp2gh 163 17 Sorting sorting NN cord-022774-wasdp2gh 163 18 and and CC cord-022774-wasdp2gh 163 19 coordinate coordinate VB cord-022774-wasdp2gh 163 20 induction induction NN cord-022774-wasdp2gh 163 21 of of IN cord-022774-wasdp2gh 163 22 three three CD cord-022774-wasdp2gh 163 23 endoplasmic endoplasmic NN cord-022774-wasdp2gh 163 24 reticulum reticulum NN cord-022774-wasdp2gh 163 25 proteins protein NNS cord-022774-wasdp2gh 163 26 containing contain VBG cord-022774-wasdp2gh 163 27 the the DT cord-022774-wasdp2gh 163 28 KDEL KDEL NNP cord-022774-wasdp2gh 163 29 retention retention NN cord-022774-wasdp2gh 163 30 signal signal NN cord-022774-wasdp2gh 163 31 Temperature temperature NN cord-022774-wasdp2gh 163 32 dependence dependence NN cord-022774-wasdp2gh 163 33 of of IN cord-022774-wasdp2gh 163 34 mast mast JJ cord-022774-wasdp2gh 163 35 cell cell NN cord-022774-wasdp2gh 163 36 histamine histamine NN cord-022774-wasdp2gh 163 37 secretion secretion NN cord-022774-wasdp2gh 163 38 Improvements improvement NNS cord-022774-wasdp2gh 163 39 in in IN cord-022774-wasdp2gh 163 40 epoxy epoxy NN cord-022774-wasdp2gh 163 41 resin resin NN cord-022774-wasdp2gh 163 42 embedding embedding NN cord-022774-wasdp2gh 163 43 methods method NNS cord-022774-wasdp2gh 164 1 Reduced reduce VBN cord-022774-wasdp2gh 164 2 temperature temperature NN cord-022774-wasdp2gh 164 3 prevents prevent VBZ cord-022774-wasdp2gh 164 4 transfer transfer NN cord-022774-wasdp2gh 164 5 of of IN cord-022774-wasdp2gh 164 6 a a DT cord-022774-wasdp2gh 164 7 membrane membrane NN cord-022774-wasdp2gh 164 8 glycoprotein glycoprotein NN cord-022774-wasdp2gh 164 9 to to IN cord-022774-wasdp2gh 164 10 the the DT cord-022774-wasdp2gh 164 11 cell cell NN cord-022774-wasdp2gh 164 12 surface surface NN cord-022774-wasdp2gh 164 13 but but CC cord-022774-wasdp2gh 164 14 does do VBZ cord-022774-wasdp2gh 164 15 not not RB cord-022774-wasdp2gh 164 16 prevent prevent VB cord-022774-wasdp2gh 164 17 terminal terminal JJ cord-022774-wasdp2gh 164 18 glycosylation glycosylation NN cord-022774-wasdp2gh 164 19 Transition transition NN cord-022774-wasdp2gh 164 20 vesicle vesicle NN cord-022774-wasdp2gh 164 21 formation formation NN cord-022774-wasdp2gh 164 22 in in IN cord-022774-wasdp2gh 164 23 vitro vitro FW cord-022774-wasdp2gh 164 24 Golgi Golgi NNP cord-022774-wasdp2gh 164 25 apparatus apparatus NN cord-022774-wasdp2gh 164 26 cisternae cisternae NN cord-022774-wasdp2gh 164 27 of of IN cord-022774-wasdp2gh 164 28 monensin monensin NNP cord-022774-wasdp2gh 164 29 - - HYPH cord-022774-wasdp2gh 164 30 treated treat VBN cord-022774-wasdp2gh 164 31 cells cell NNS cord-022774-wasdp2gh 164 32 accumulate accumulate VBP cord-022774-wasdp2gh 164 33 in in IN cord-022774-wasdp2gh 164 34 the the DT cord-022774-wasdp2gh 164 35 cytoplasm cytoplasm NN cord-022774-wasdp2gh 164 36 of of IN cord-022774-wasdp2gh 164 37 liver liver NN cord-022774-wasdp2gh 164 38 slices slice NNS cord-022774-wasdp2gh 165 1 Intracellular Intracellular NNP cord-022774-wasdp2gh 165 2 membrane membrane NN cord-022774-wasdp2gh 165 3 flow flow NN cord-022774-wasdp2gh 165 4 : : : cord-022774-wasdp2gh 166 1 reconstitution reconstitution NN cord-022774-wasdp2gh 166 2 of of IN cord-022774-wasdp2gh 166 3 transition transition NN cord-022774-wasdp2gh 166 4 vesicle vesicle NN cord-022774-wasdp2gh 166 5 formation formation NN cord-022774-wasdp2gh 166 6 and and CC cord-022774-wasdp2gh 166 7 function function VB cord-022774-wasdp2gh 166 8 in in IN cord-022774-wasdp2gh 166 9 a a DT cord-022774-wasdp2gh 166 10 cellfree cellfree NN cord-022774-wasdp2gh 166 11 system system NN cord-022774-wasdp2gh 166 12 Isolation isolation NN cord-022774-wasdp2gh 166 13 of of IN cord-022774-wasdp2gh 166 14 a a DT cord-022774-wasdp2gh 166 15 vesicular vesicular JJ cord-022774-wasdp2gh 166 16 intermediate intermediate NN cord-022774-wasdp2gh 166 17 in in IN cord-022774-wasdp2gh 166 18 the the DT cord-022774-wasdp2gh 166 19 cell cell NN cord-022774-wasdp2gh 166 20 - - HYPH cord-022774-wasdp2gh 166 21 free free JJ cord-022774-wasdp2gh 166 22 transfer transfer NN cord-022774-wasdp2gh 166 23 of of IN cord-022774-wasdp2gh 166 24 membrane membrane NN cord-022774-wasdp2gh 166 25 from from IN cord-022774-wasdp2gh 166 26 transitional transitional JJ cord-022774-wasdp2gh 166 27 elements element NNS cord-022774-wasdp2gh 166 28 of of IN cord-022774-wasdp2gh 166 29 the the DT cord-022774-wasdp2gh 166 30 endoplasmic endoplasmic NN cord-022774-wasdp2gh 166 31 reticulum reticulum NN cord-022774-wasdp2gh 166 32 to to IN cord-022774-wasdp2gh 166 33 Golgi Golgi NNP cord-022774-wasdp2gh 166 34 apparatus apparatus NN cord-022774-wasdp2gh 166 35 cisternae cisternae NN cord-022774-wasdp2gh 166 36 of of IN cord-022774-wasdp2gh 166 37 rat rat NN cord-022774-wasdp2gh 166 38 liver liver NN cord-022774-wasdp2gh 166 39 Pre Pre NNP cord-022774-wasdp2gh 166 40 - - : cord-022774-wasdp2gh 166 41 and and CC cord-022774-wasdp2gh 166 42 post post JJ cord-022774-wasdp2gh 166 43 - - JJ cord-022774-wasdp2gh 166 44 Golgi golgi JJ cord-022774-wasdp2gh 166 45 vacuoles vacuole NNS cord-022774-wasdp2gh 166 46 operate operate VBP cord-022774-wasdp2gh 166 47 in in IN cord-022774-wasdp2gh 166 48 the the DT cord-022774-wasdp2gh 166 49 transport transport NN cord-022774-wasdp2gh 166 50 of of IN cord-022774-wasdp2gh 166 51 Semliki Semliki NNP cord-022774-wasdp2gh 166 52 forest forest NN cord-022774-wasdp2gh 166 53 virus virus NN cord-022774-wasdp2gh 166 54 membrmke membrmke JJ cord-022774-wasdp2gh 166 55 glycoproteins glycoprotein NNS cord-022774-wasdp2gh 166 56 to to IN cord-022774-wasdp2gh 166 57 the the DT cord-022774-wasdp2gh 166 58 cell cell NN cord-022774-wasdp2gh 166 59 surface surface NN cord-022774-wasdp2gh 166 60 Temperature temperature NN cord-022774-wasdp2gh 166 61 sensitive sensitive JJ cord-022774-wasdp2gh 166 62 steps step NNS cord-022774-wasdp2gh 166 63 in in IN cord-022774-wasdp2gh 166 64 the the DT cord-022774-wasdp2gh 166 65 transport transport NN cord-022774-wasdp2gh 166 66 of of IN cord-022774-wasdp2gh 166 67 secretory secretory JJ cord-022774-wasdp2gh 166 68 proteins protein NNS cord-022774-wasdp2gh 166 69 through through IN cord-022774-wasdp2gh 166 70 the the DT cord-022774-wasdp2gh 166 71 Golgi Golgi NNP cord-022774-wasdp2gh 166 72 complex complex NN cord-022774-wasdp2gh 166 73 in in IN cord-022774-wasdp2gh 166 74 exocrine exocrine JJ cord-022774-wasdp2gh 166 75 pancreatic pancreatic NN cord-022774-wasdp2gh 166 76 cells cell NNS cord-022774-wasdp2gh 166 77 Temperature temperature NN cord-022774-wasdp2gh 166 78 and and CC cord-022774-wasdp2gh 166 79 energy energy NN cord-022774-wasdp2gh 166 80 dependence dependence NN cord-022774-wasdp2gh 166 81 of of IN cord-022774-wasdp2gh 166 82 secretory secretory JJ cord-022774-wasdp2gh 166 83 protein protein NN cord-022774-wasdp2gh 166 84 transport transport NN cord-022774-wasdp2gh 166 85 in in IN cord-022774-wasdp2gh 166 86 the the DT cord-022774-wasdp2gh 166 87 exoc exoc NNP cord-022774-wasdp2gh 166 88 ~ ~ NFP cord-022774-wasdp2gh 166 89 ne ne NNP cord-022774-wasdp2gh 166 90 pancreas pancrea VBZ cord-022774-wasdp2gh 166 91 Replication Replication NNP cord-022774-wasdp2gh 166 92 of of IN cord-022774-wasdp2gh 166 93 coronavirus coronavirus NN cord-022774-wasdp2gh 166 94 MHV mhv NN cord-022774-wasdp2gh 166 95 - - HYPH cord-022774-wasdp2gh 166 96 A59 A59 NNP cord-022774-wasdp2gh 166 97 in in IN cord-022774-wasdp2gh 166 98 sac(- sac(- NNP cord-022774-wasdp2gh 166 99 ) ) -RRB- cord-022774-wasdp2gh 166 100 cells cell NNS cord-022774-wasdp2gh 166 101 : : : cord-022774-wasdp2gh 166 102 determination determination NN cord-022774-wasdp2gh 166 103 of of IN cord-022774-wasdp2gh 166 104 the the DT cord-022774-wasdp2gh 166 105 21 21 CD cord-022774-wasdp2gh 166 106 22 22 CD cord-022774-wasdp2gh 166 107 first first JJ cord-022774-wasdp2gh 166 108 site site NN cord-022774-wasdp2gh 166 109 of of IN cord-022774-wasdp2gh 166 110 budding budding NN cord-022774-wasdp2gh 166 111 of of IN cord-022774-wasdp2gh 166 112 progeny progeny JJ cord-022774-wasdp2gh 166 113 virions virion NNS cord-022774-wasdp2gh 166 114 Site site NN cord-022774-wasdp2gh 166 115 of of IN cord-022774-wasdp2gh 166 116 addition addition NN cord-022774-wasdp2gh 166 117 of of IN cord-022774-wasdp2gh 166 118 N n NN cord-022774-wasdp2gh 166 119 - - HYPH cord-022774-wasdp2gh 166 120 acetylgalactosamine acetylgalactosamine NNP cord-022774-wasdp2gh 166 121 to to IN cord-022774-wasdp2gh 166 122 the the DT cord-022774-wasdp2gh 166 123 E1 e1 NN cord-022774-wasdp2gh 166 124 glycoprotein glycoprotein NN cord-022774-wasdp2gh 166 125 of of IN cord-022774-wasdp2gh 166 126 mouse mouse NN cord-022774-wasdp2gh 166 127 hepatitis hepatitis NN cord-022774-wasdp2gh 166 128 virus virus NN cord-022774-wasdp2gh 166 129 - - : cord-022774-wasdp2gh 166 130 A59 A59 NNP cord-022774-wasdp2gh 166 131 0966 0966 CD cord-022774-wasdp2gh 166 132 ) ) -RRB- cord-022774-wasdp2gh 167 1 Interferon interferon NN cord-022774-wasdp2gh 167 2 production production NN cord-022774-wasdp2gh 167 3 by by IN cord-022774-wasdp2gh 167 4 inactivated inactivate VBN cord-022774-wasdp2gh 167 5 Newcastle Newcastle NNP cord-022774-wasdp2gh 167 6 Disease Disease NNP cord-022774-wasdp2gh 167 7 virus virus NN cord-022774-wasdp2gh 167 8 in in IN cord-022774-wasdp2gh 167 9 cell cell NN cord-022774-wasdp2gh 167 10 cultures culture NNS cord-022774-wasdp2gh 167 11 and and CC cord-022774-wasdp2gh 167 12 in in IN cord-022774-wasdp2gh 167 13 mice mouse NNS cord-022774-wasdp2gh 168 1 This this DT cord-022774-wasdp2gh 168 2 work work NN cord-022774-wasdp2gh 168 3 was be VBD cord-022774-wasdp2gh 168 4 supported support VBN cord-022774-wasdp2gh 168 5 in in IN cord-022774-wasdp2gh 168 6 part part NN cord-022774-wasdp2gh 168 7 by by IN cord-022774-wasdp2gh 168 8 a a DT cord-022774-wasdp2gh 168 9 grant grant NN cord-022774-wasdp2gh 168 10 from from IN cord-022774-wasdp2gh 168 11 the the DT cord-022774-wasdp2gh 168 12 National National NNP cord-022774-wasdp2gh 168 13 Institutes Institutes NNPS cord-022774-wasdp2gh 168 14 of of IN cord-022774-wasdp2gh 168 15 Health Health NNP cord-022774-wasdp2gh 168 16 HD HD NNP cord-022774-wasdp2gh 168 17 11508 11508 CD cord-022774-wasdp2gh 168 18 . . . cord-022774-wasdp2gh 169 1 We -PRON- PRP cord-022774-wasdp2gh 169 2 thank thank VBP cord-022774-wasdp2gh 169 3 Charles Charles NNP cord-022774-wasdp2gh 169 4 Bracker Bracker NNP cord-022774-wasdp2gh 169 5 for for IN cord-022774-wasdp2gh 169 6 use use NN cord-022774-wasdp2gh 169 7 of of IN cord-022774-wasdp2gh 169 8 electron electron NN cord-022774-wasdp2gh 169 9 microscope microscope NN cord-022774-wasdp2gh 169 10 facilities facility NNS cord-022774-wasdp2gh 169 11 , , , cord-022774-wasdp2gh 169 12 Dorothy Dorothy NNP cord-022774-wasdp2gh 169 13 Werderitsh Werderitsh NNP cord-022774-wasdp2gh 169 14 for for IN cord-022774-wasdp2gh 169 15 excellent excellent JJ cord-022774-wasdp2gh 169 16 technical technical JJ cord-022774-wasdp2gh 169 17 assistance assistance NN cord-022774-wasdp2gh 169 18 and and CC cord-022774-wasdp2gh 169 19 Olympia Olympia NNP cord-022774-wasdp2gh 169 20 Mejia Mejia NNP cord-022774-wasdp2gh 169 21 for for IN cord-022774-wasdp2gh 169 22 help help NN cord-022774-wasdp2gh 169 23 with with IN cord-022774-wasdp2gh 169 24 preliminary preliminary JJ cord-022774-wasdp2gh 169 25 experiments experiment NNS cord-022774-wasdp2gh 169 26 . . . cord-022774-wasdp2gh 170 1 The the DT cord-022774-wasdp2gh 170 2 participation participation NN cord-022774-wasdp2gh 170 3 of of IN cord-022774-wasdp2gh 170 4 Angela Angela NNP cord-022774-wasdp2gh 170 5 Downham Downham NNP cord-022774-wasdp2gh 170 6 and and CC cord-022774-wasdp2gh 170 7 Victoria Victoria NNP cord-022774-wasdp2gh 170 8 Vucich Vucich NNP cord-022774-wasdp2gh 170 9 in in IN cord-022774-wasdp2gh 170 10 a a DT cord-022774-wasdp2gh 170 11 portion portion NN cord-022774-wasdp2gh 170 12 of of IN cord-022774-wasdp2gh 170 13 the the DT cord-022774-wasdp2gh 170 14 study study NN cord-022774-wasdp2gh 170 15 is be VBZ cord-022774-wasdp2gh 170 16 gratefully gratefully RB cord-022774-wasdp2gh 170 17 acknowledged acknowledge VBN cord-022774-wasdp2gh 170 18 . . .